From 1988 to 1995, 148 patients with varicosis of lower extremity were treated by local i-jection with 40% urea. The results were good. The mechanism of urea injection for treating the varicosis of lower extreatment was discussed.The urea therapy is one of better methods for the varicosis of lower extremity.

Objective To apply daily goal health education in the inpatients with stroke,to observe the influence on cognitive attitude to the disease and treatment compliance of patients.Methods A total of 133 cases of inpatients with ischemic stroke were included,which were divided into experimental group (n=67) and control group (n=66),experimental group conducted with daily goal health education mode,while the control group with conventional health education mode.Cognitive attitude to the disease and treatment compliance were all compared in patients when discharged.Results After health education in two ways,scores of disease treatment attitude and social participation attitude of the experimental group [(12.15±2.61) points and (5.70±1.60) points] was higher than the control group [(11.33±1.91) points and (5.02±1.49) points],the difference was statistically significant (t=2.059,2.561,P＜0.05),treatment compliance was higher than control group (x2=6.130-7.063,P＜0.05).Conclusions Daily goal health education can significandy improve the cognitive attitude and the treatment compliance of patients with stoke,which is a good health education mode to the inpatients with stroke.

The main purpose of this work is to prepare self-emulsifying drug delivery system (SEDDS) of a poorly water soluble drug, puerarin. Solubility of puerarin was determined in various oils and surfactants. Oleic acid and Tween 80 provided higher solubility. Addition of propylene glycol as cosurfactant improved solubility of puerarin and the spontaneity of self-emulsification. A series of mixtures comprising oleic acid, propylene glycol and Tween 80 were prepared and their self-emulsifying properties were studied. Pseudo-ternary phase diagrams were constructed to identify the efficient self-emulsification region and particle sizes of the resultant emulsions were determined using a laser diffraction sizer. The harmacokinetic behaviors of three different SEDDS formulations (F2, F3, F4) were investigated in Beagle dogs. The bioavailability was compared using the pharmacokinetic parameters, peak plasma concentration (Cmax), time to reach peak plasma concentration (Tmax) and total area under the plasma concentration-time curve (AUC0-t). AUC0-t was significantly higher in formulation F2 group (5.201±0.511) ng·mL-1·h and formulation F3 group (5.174±0.498) ng·mL-1·h than that in formulation F4 group (3.013±0.623) ng·mL-1·h. Also, Cmax was significantly higher in formulation F2 group (1.524±0.125) ng·mL-1 and formulation F3 group (1.513±0.157) ng·mL-1 than that in formulation F4 group (0.939±0.089) ng·mL-1. Further analysis of the data showed a statistically significant difference between F2 and F4 (P<0.01) as well as F3 and F4 (P<0.01) with regard to the values of AUC0-∞ and Cmax for three SEDDS formulations, but not between those of F2 and F3 (P>0.05). From these studies, the SEDDS formulation containing oleic acid (17.5%), Tween 80 (34.5%) and propylene glycol (34.5%) (w/w) was selected as an optimized SEDDS formulation of puerarin. The data suggest the potential use of SEDDS to improve oral absorption of puerarin.

OBJECTIVE: To screen the best macroporous adsorptive resin for separation and purification of flower Elaeagnus angustifolia flavonoids and to establish the purification process parameters. METHODS: Using macroporous adsorption resin adsorption rate and desorption rate as the indexes, the best resin was screened out for enriching flower Elaeagnus angustifolia flavonoids. The optimal process conditions were investigated through single factor test. RESULTS: DA201 resin showed good adsorption and separation property for Elaeagnus angustifolia flavonoids. The detailed process conditions were as follows: the ratio of diameter to height of resin column was 1:8, sample loading rate was 0.5 mL·min-1, sample concentration was 20 mg·mL-1, pH was adjusted to 4, with elution rate of 0.5 mL·min-1, the largest sample load was 4.5 BV, eluent concentration was 80% and the dosage was 12 BV. CONCLUSION: DA201 type resin, showed good purification effect for flavonoids Elaeagnus angustifolia in the defined process conditions with a yield of 85.23%. The purity of total flavonoids achieved 14.95%, which was 3 times of that by coarse extraction (4.901%). Copyright 2012 by the Chinese Pharmaceutical Association.

OBJECTIVE:To evaluate the trend of drug use in22hospitals in Hangzhou region.METHODS:The medi?cation data obtained from22hospitals in Hangzhou region during the period from2002to2004were statistically analyzed.RESULTS:The total amount of money for drug purchase has been steadily increasing,but the growth rate has begun to de?crease;The amount of money for purchase of anti-infective agents always ranked the first but the percentage declined year by year;The purchase of domestic drugs increased by a wide margin and accounted almost50%of the total market consumption.CONCLUSION:The money used in purchase of anti-malignancy drug,drug for cardiovascular system,and vitamins tends to increase.

Objective To study the clinical characteristics and effects of surgical treatment of children with thalamic tumors.Methods The clinical data of 22 cases were retrospectively studied and followed-up 6 months to 9 years.Results There were 13 boys and 9 girls,their ages ranging from 3 to 13 years.The average duration of symptoms before diagnosis about 2 months.Headache and papilledem were the most symptoms and signs,respectively.Most children′s thalamic tumors were low grade tumors with clear verge.In this group,good results were obtained that total remove 9 cases,subtotal remove 8 cases,partial remove 3 cases,biopsy 2 cases and no surgical death.Conclusions Clinical character of children thalamic tumors is distinct and good surgical results in the nearly future.The long results are determined by type of pathology.

Objective To observe the effects of treating knee osteoarthritis(KOA)combined with synovitis with Gubiqing,and discuss its mechanism.Methods A total of 60 cases with KOA were randomly recruited into a control group and a treatment group,30 cases in each.TCM symptoms,signs and health assessment questionnaire(HAQ)were observed before and after the treatment.Results The tohal therapeutic effect was 90%and 70%in the treatment group and the control group respectively.There was significant difference between the two group(χ~2=48,P=0.003).Body signs and HAQ were also greatly improved in the treatment group(t=0.004、P=0.008).Conclusion Gubiqing can not only restrain chondrocyte apoptosis in knee osteoarthritis combined with synovitis,but also relive the damage of articular cartilage.

Objective To explore the pattern of cognitive impairments mainly caused by the white matter lesions(WML) in the frontal lobe in patients with subcortical ischemic vascular disease (SIVD).Methods Fifty SIVD patients were divided into severe WML group (visual score >3, n=27) and mild WML group(visual score ≤3, n=23) according to their severities of the WML in frontal lobe .Seven patients without SIVD were collected as controls .All patients underwent a set of neuropsychological battery ,and the results were analyzed .Results There was no statistical significance among three groups on basic data .Compared with mild WML group and control group , non frontal white matter scores and numbers of lacunes in frontal lobe of severe WML group were significantly higher ( all P=0.000).Compared with mild WML group,the Montreal cognitive assessment scale in severe WML group were significantly lower ( P=0.047 ) , and scores related to the executive function were significantly lower ( P=0.006 ) , even after adjusting the numbers of lacunes in frontal lobe ,there was statistically significant difference (P=0.038). Multiple regression confirmed that the Z scores of executive functions were mainly affected by white matter lesions located in the frontal lobe ( P=0.000 ) .Conclusion WML located in the frontal lobe mainly affect the executive function in patients with SIVD .

Objective To isolate and identificate a specific new gene highly related to human bladder cancer and to investigate the carcinogenesis mechanism and genetic susceptibility. Methods Normal bladder epithelia and cancer tissue collected from 8 cases of primary bladder transitional cell carcinoma were studied using the high performance?sensitive technique of mRNA differential display.The differential fragments were found by comparing with genetic expression. 6 of them were reamplificated?cloned?sequenced,and performed the autoploidy index in the public database of genbank. Results (1)37 differential fragments have been isolated.36 of which are expressed or highly expressed in cancer tissues,but not so or lowly expressed in normal tissues;while it has been expressed in 1 normal tissue but not in the cancer tissue.(2)Results of indexing:1 is homologous to TBX3 (T box gene),autoploidy 99%.1 is homologous to cl.Ca4 gene (member 4 of Calcium activated chloride channel gene family), autoploidy 99%.1 is homologous to Cu,Zn SOD gene (Cu,Zn superoxide dismutase),99%.The remaining fragments are not homologous to unknown sequences in the public database of genbank,assigned as YHHQ1,XHL,LHX. Conclusions (1)It is verified at genetic level the relation between the conment's increasing of Cu,Zn SOD in bladder cancer derived tissue and the occurrence,advance of bladder cancer.(2)The relation between TBX3,cl.Ca4 gene and human carcinoma has been observed.(3)YHHQ1 may be the new candidate of anti oncogene related to bladder cancer.XHL and LHX may be the new candidates of oncogene related to bladder cancer.

Objective To explore the effect of intrahepatic injection of liposome-mediated VEGF plasmid on ischemia-reper-fusion liver injury and its mechanism. Methods Rabbits were randomly divided into normal group, ischemia-reperfusion group and recombinant VEGF therapy group( liposome-mediated transfer of VEGF plasmid into liver via portal vein 20 min before ischemia of liver). The model of liver ischemia-reperfusion injury was established. Liver function and the activity of SOD.XO in blood were determined at the 0,2nd,6th,12th,and 24th h after operation. RT-PCR technique was applied to detect the expression level of Fas mRNA in liver tissues of every group,and flow cytometry was used to measure cell apoptosis rate at the 6th h after operation. At the 24th h after operation,all rabbits were killed and liver tissues of ischemia were taken to make pathological sections for observing the morphology and microstructure under the light microscopy and electron microscopy. ResuJts The level of ALT in recombinant VEGF therapy group was markedly reduced as compared with ischemia-reperfusion group at the 6th,12th,and 24th h after operation( P<0. 05). The activity of SOD in recombinant VEGF therapy group was significantly higher than in ischemia-reperfusion group at the 6th, 12th,and 24th h after operation. The activity of XO in recombinant VEGF therapy group was significantly lower than that in ischemia-reperfusion group at the 6th,12th,and 24th h after operation(P< 0. 05 or P<0. 01). In addition,there was significant difference in the expression of Fas mRNA and cell apoptosis rate between recombinant VEGF therapy group and ischemia-reperfusion group(P<0. 01). The injury of hepatocytes in recombinant VEGF therapy group was significantly alleviated as compared with that in ischemia-reperfusion group under the light microscopy and e-lectron microscopy. Conclusion Liposome-mediated transfer of VEGF plasmid into liver before ischemia of liver can obviously protect hepatocytes by increasing anti-oxidative ability, decreasing the expression of Fas mRNA, and finally inhibiting hepato-cyte apoptosis.