Osteosarcoma is the most common primary malignant tumor that develops in bone. Since chemotherapy was introduced into the multi-modality treatment regimen for high-grade osteosarcoma, its prognosis has been impressively improved; about two-thirds of all patients could achieve long-term survival. This review summarized the history of chemotherapy for osteosarcoma and current chemotherapeutic treatment strategies for classical osteosarcoma. The review also emphasized the necessity that the patients should be treated in specialized centers and prospective, multi-institutional trials are needed.

Objective To investigate the effect of compound preparation of Astragalus mongho-licus on subsets of lymphocytes in patients with myasthenia gravis (MG), and to explore its regulatory mechanism. Methods 60 MG patients were randomly divided into test group (n=30, given compoundpreparation of Astragalus mongholicus for 12 wks) and control group (n=30, given prednisone tablets for 12 wks). Flow cytometry was applied to examining the distribution change of the subsets of lym-phocytes in patients before and after treatment. Results After treatment, the percentages of CD4+ T cells and the ratio of CD4+/CD8+ in both groups decreased compared with those before treatment.The ratio of CD4+/CD8+ in test group was significantly lower than that in control group (P<0.05).The percentage of CD8+ T cells in test group after treatment increased significantly (P<0.05) com-pared with that before treatment, but had no significant difference compared with that in control group(P>0. 05). Conclusion The result from the current study suggests that one of the mechanisms of the compound preparation of Astragalus monghoticus regulating immune response may be achieved through its modulating effect on the distribution of subsets of lymphoeytes and humoral immune func-tion.

Automatic Data Processing ; Humans ; Medicine, Chinese Traditional ; Pulse

Most of the soil microorganisms are unculturable,which limits the exploitation and application of microbial resources.Metagenomics has great potential in the research of unculturable microorganisms in soil.Progress on the research of metagenomic DNA purification,metagenomic library construction and screening of soil microorganisms are reviewed briefly.

Peripheral T-cell lymphoma (PTCL) is a kind of highly aggressive lymphoma which contains a collection of subgroups. The patients with PTCL have poor prognosis under current chemotherapy regimens, so the treatment of PTCL remains a big challenge. Brentuximab vedotin combined with CHP regimen, pralatrexate in combination with romidepsin and other regimens have set up more options for patients with PTCL. Novel agents, such as PI3K inhibitors, which entered into clinical trials, bring good news to PTCL patients.

Normative development of human assisted reproduction technology not only depends on ethics con-sciousness of the employee, effective system guarantee and perfect supervision system but also depends on ethics consciousness of infertile couples. Popularizing the knowledge of reproductive ethics for infertile couples and foste-ring their ethics consciousness is a vital problem of normative development of human assisted reproduction technolo-gy and an effective way to solve the ethical issues of human assisted reproduction technology.

In order to evaluate the use of transendoscopic miniature ultrasonic probe (TEMP) in the diagnosis of gastric submucosal tumor, 18 patients were investigated. The results obtained by TEMP were compared with those of gastroscopy, and endoscopical ultrasonography (EVS). The result showed that among those 18 patients, gastric submucosal leiomyomas, were common and accounted for 88.9％. Most of them were intramural. The diagnostic accuracy of TEMP for the size of tumor and gastric submucosal tumor was 88.9％ and 100％ respectively. Which were much higher than those of gastroscopy (33.3％ and 61.1％ respectively). The sonogrophic characteristics of gastric submucosal tumors in TEMP imaging were related to the nature of the tumors, hut the common manifestations were that all the tumors were hypoechoic under the hyperechoic mucosal layer with clear bell shaped origin, and usually appeared as round or nearly round intragastric eminence. It is concluded that the selective use of TEMP has great value in the diagnosis of gastric submucosal turmor, especially for those lesions which were small and difficult to be detected by EUS.

Objective To explore the influence of massage on gastrointestinal motility in premature infants.Methods Fifty-eight premature infants from NICU of the affiliated hospital of Jining medical college were randomly divided into massage group(28 cases) and control group(30 cases).They were all received usual care.The infants in massage group were subjected to caresses touches twice(5-20 min/time)every day.The time of meconium and charcoal being excreted were recorded.Blood samples were collected on day 2,7 after birth.Motolin and gastrin were determined by radioimmunassays.Results The time of meconium and charcoal being excreted in massage group was(78.78?41.16) h and(21.48?9.85) h,respectively.Those in control group were(101.84?32.84) h and(34.66?13.20) h,respectively,there were significant differences between massage group and control group(P0.05).The blood concentrations of motilin and gastrin on day 7 in massage group were(402.35?40.14) ?g/L and (133.60?25.99) ?g/L, respectively,those were(315.84?37.62) ?g/L and (80.22?33.50) ?g/L in control group,respectively,there were significant differences in the blood concentrations of motilin and gastrin between 2 groups(Pa

Objective To investigate the expression of spleen tyrosine kinase (Syk) in endometrial cancer ( EC ) and its relationship with the clinic and pathological factors , as well as the diagnostic value for EC . Methods Through real-time PCR and immunohistochemistry, the expression level of Sky mRNA and protein in normal endometrial tissues, atypical hyperplasia of endometrial tissues and endometrial cancer tissues were examined. Results The expression of Syk in EC were lower than that in endometrial atypical hyperplasia and normal endometrium (P < 0.05). The expression of Syk was related to histological grade, invasive depth, lymphatic metastasis and clinical stages (P<0.05). Conclusion Syk contributes to tumorigenesis and metastasis of EC. The detection of Syk can provide evidence to evaluate the malignancy grade of EC.

Objective To conduct the comparative study of the measurement of breast volume by MRI imaging and Archimedes method in immediate implant breast reconstruction.Methods A total of 44 patients who were diagnosed as breast cancer and undergone immediate implant breast reconstruction were selected from March 2011 to March 2013.22 cases were guided to select suitable breast implants by measuring the breast volume and correlative radial line based on MRI imaging.Control group containing 22 cases were guided to select breast implants by traditional Archimedes method and clinical experience.3 breast surgeons and plastic surgeons who did not participate in the operation were selected to judge the postoperative breast shape.Data of two groups were analyzed by using chisquare test.Results All the patients recovered smoothly after operation without infection,and the wound healed well.The breast shape was evaluated.21 cases (95.5 ％) were good in test group and 1 case (4.5％) was poor;16 cases (72.7％) were good in control group and 6 cases (27.3) were poor.The comparison between the test group and control group had statistical significance (P＜0.05).Conclusions The method to measure the breast volume and correlative radial line based on MRI imaging has important values for selecting breast implants in immediate implant breast reconstruction.It could be extensively used in clinical practice.