Chylous Ascites ; congenital ; therapy ; Humans ; Infant ; Male ; Parenteral Nutrition, Total ; Somatostatin ; therapeutic use

Reflux is a common post-gastrectomy complication due to the destruction of physiological antireflux mechanism,which affects patients' post-surgery life quality greatly.It's one of the basic requirements of digestive tract reconstruction to reduce the incidence of post-surgery reflux,and nowadays there are many kinds of anti-reflux surgical methods,which are also hot topics both at home and abroad.The non-surgical anti-reflux treatments are mainly focused on gastrointestinal motility promotion,mucosa protection,acid suppression,and so on.There are still some controversies concerned with which surgical or non-surgical way to choose.It's of great significance to have a deep insight into the associated mechanisms and make a wise decision on the surgical management with proper non-surgical assistance.

Objective To investigate the neurotoxicity of intrathecal (IT) doxepin in rats.Methods Twenty-four adult male Wistar rats weighing 250-300 g in which intrathecal micro-catheter was successfully implanted without complications were randomly divided into 4 groups ( n =6 each):control group ( group N ) and 3 doxepin groups receiving IT doxepin 40,20 and 10 mmol/L 0.2 μl/g respectively (groups D40,D20,D10 ).In N group normal saline was injected IT instead of doxepin.The animals were killed at 6 h after IT administration.The lumbar segment of the spinal cord was removed for microscopic examination and determination of myelin basic protein (MBP) content in the spinal cord using a double-antibody sandwich (ELISA).Results The severity of neuronal damage and decrease in MBP content in the spinal cord induced by IT doxepin were dose-dependent.Conclusion The neurotoxicity induced by IT doxepin is dose-dependent.

Objective To study the correlation between diabetes and the new discovered angiopietin-related growth factor (AGF)that participates in energy metabolism.MethodsA total of 24 male C.57BL/6 mice were divided into three groups:controlled group; fed with high-fat diet( HFD); fed with high-fat diet for 10 weeks and then treated with rosiglitazone ( HFD + RSG) ; The relative level of AGF mRNA expression in livers was measured by determining a ratio of PCR products of AGF to that of β-actin gene.Oral glucose tolerance test,and insulin resistance index (HOMA-IR) were used to measure blood glucose level and insulin sensitivity.Results Compared with the controls,the expression of AGF mRNA in liver decreased significantly in HFD group( P ＜0.05),and increased significantly in HFD + RSG group( P＜0.01 ).A negative correlation was found between AGF mRNA expression in mice livers and HOMA score ( r =-0.516,P ＜ 0.05 ).Conclusions AGF as a new hepatocyte derived circulating factor counteracts obesity and is related to insulin resistance.

The plasmids of recombinant suicide vector containing the truncated stn gene and the deleted stn gene were constructed. The Salmonella stn-deficient mutant was prepared by homologous recombination between the mutant stn gene in the recombinant suicide vector and the wild-type gene in the chromosome of S. Typhimurium 2000. The detection of biological activity of stn gene production in those mutants indicated that Salmonella stn-deficient mutants evoked significantly less fluid secretion in mouse intestinal loops compared to that seen with wild-type Salmonella. Upon oral challenge of mice, the fifty percent lethal dose of the Salmonella stn-deficient mutants was greater than that for the wild-type bacteria. Those studies showed that the stn gene is very important factor in the pathogenesis of salmonellosis.

Objective　To explore the changes and significance of the circulating immunocomplexes(CIC) in patients with chronic iridocyclitis.Methods　We assessed circulating immunocomplexes in peripheral blood of 45 patients with recurrent uveitis and 42 healthy controls by PEG deposition with the biochemical analyzer to obtain levels of CIC different with recurrent uveitis.Results　Levels of CIC were significantly higher in active patients(27.358±6.729)than those in controls(13.459±6.164,P＜0.001) and inactive patients (16.248±6.233,P＜0.001),but patients (inactive) had significantly higher levels than those in controls (P＜0.05).Conclusion　Levels of CIC were found in the groups of active patients with recurrent uveitis (27.358±6.729), but levels of CIC in inactive patients fell. The result of this study suggest that recurrence of uveitis is related to immunocomplexes,and changes in the levels of CIC could be also a measure standard of therapeutic effects.

Objective To investigate the early diagnosis of sudden cardiac death so as to increase the resuscitating success rate.Methods 24 cases with sudden cardiac death were analyzed.Results In 24 cases,there were 10 cases resuscitated successfully,8 cases with heartbeats recovered died in 1～3 days,5 of which had average blood glucose of 21.6 mmol/L and blood sodium of 156 mmol/L,and 6 cases resuscitated unsuccessfully.Conclusion 70.83% was caused by AMI in 24 cases.The early diagnosis of AMI is mainly based on clinical manifestation.Althogh early ECG did not show it,AMI should not be denied and misdiagnosed.Resuscitation should be performed on the spot as soon as cardiac arrest occurs.Naloxone and NaHCO3 should be used rationally.Meanwhile,be sure to prevent and treat reperfusion-injury.The prognosis would be extremely poor if the patient showed hyperglycemia and hypernatremia during resuscitation.

Objective To investigate the bacterial distribution and drug sensitivity characteristics of chronic suppurative sinusitis.Methods Nasal secretions of 89 cases of chronic suppurative sinusitis patients before operation were collected in this study,and nasal secretions were culture in medium.Disk diffusion method (Kirby-Bauer,K-B)was used for drug sensitivity test.Sensitivity of bacteria strain to cefazolin,ceftazidime,cefepime,imipenem,mero-penem,vancomycin,cefoperazone -sulbactam,ciprofloxacin and other antibiotics were analyzed.Results After the submission of purulent secretion specimens of nasal meatus in 89 cases chronic suppurative sinusitis patients with sticky,we found that 80 of the patients were detected bacteria.The positive rate was 89.89% (80/89).80 patients were detected in 82 strains of bacteria,a total of 15 species,of which 1 cases of nasal meatus sticky purulent secretion specimens there were 2 strains of bacteria.In 15 kinds of staphylococcus aureus were detected bacteria,staphylococcus aureus,escherichia coli,pseudomonas,enterobacter aerogenes,5 kinds of bacteria occupy the top 5,respectively, accounted for 21.95%,18.29%,14.63%,13.41%,10.98%.Drug sensitivity test of staphylococcus aureus showed that all of the staphylococcus aureus was sensitive to vancomycin,the sensitivity was 100%;the sensitivity of cefazo-lin,ceftazidime,cefepime,imipenem,meropenem,cefoperazone sulbactam,ciprofloxacin and other antibiotics were 0.00%.All the staphylococcus epidermidis were sensitive to imipenem,meropenem,vancomycin,cefoperazone sulbac-tam,the sensitivity was 100.00%;followed by cefazolin,the sensitivity was 86.67%,and to ceftazidime,cefepime, ciprofloxacin sensitive,were all below 30.00%.Escherichia coli,pseudomonas aeruginosa and enterobacter aerogenes and other gram negative bacteria were sensitive to imipenem,meropenem,the sensitivity was 100.00%.Conclusion The bacterial infection in patients with chronic suppurative sinusitis pathogens,including staphylococcus epidermidis, staphylococcus aureus,escherichia coli,pseudomonas,enterobacter aerogenes,bacterial culture positive to vancomy-cin,imipenem,meropenem,cefoperazone Shubatan,cefazolin and antimicrobial susceptibility,and so on antimicrobial treatment but should closely monitor the antimicrobial spectrum of change,in order to adjust the medication.

Background:Inflammatory bowel disease(IBD)is a chronic,non-specific,relapsing inflammatory bowel disorder. Prediction of prognosis and relapse is of great importance for disease control. Aims:To analyze the characteristics and related factors for relapse in IBD patients. Methods:Four hundred and seventy-nine hospitalized patients diagnosed as ulcerative colitis(UC,n = 301)or Crohn’s disease( CD,n = 178)from Jan. 2004 to Jun. 2014 were enrolled and divided into relapse group and non-relapse group. The clinical characteristics were collected and univariate and multivariate analysis were performed to identify the influencing factors for relapse. Results:The relapse rates for UC and CD were 52. 49% and 77. 53% ,respectively. In stepwise Logistic regression analysis,younger onset age( < 40 years old,OR =0. 825,95% CI:0. 777-0. 876,P = 0. 000),longer disease duration(OR = 1. 186,95% CI:1. 089-1. 291,P = 0. 000) and extensive colitis(OR = 16. 993,95% CI:2. 670-108. 170,P = 0. 003)were independent risk factors for relapse of UC,while younger onset age( < 40 years old,OR = 0. 944,95% CI:0. 915-0. 974,P = 0. 000)and poor medication adherence(OR = 31. 074,95% CI:10. 456-92. 343,P = 0. 000)were independent risk factors for relapse of CD. Conclusions:Patients at high risk for relapse of IBD can be identified by referring to the related risk factors. Early identification and intervention,persistence for maintenance therapy and improving medication adherence by health education may reduce the clinical relapse of IBD.

Magnetic iron oxide nanoparticles (MIONs) have been widely used in biomedical fields, including targeted drug deliv-ery, magnetic resonance imaging, separation and enrichment of cancer cells, and tumor-targeted therapy. However, MIONs are increas-ingly necessary to ensure reduced toxicity, more stable colloidal dispersion, better biocompatibility, and higher magnetic responsive-ness. Thus, apart from the continuous improvement of material synthesis, specific surface modification of MIONs is essential to choose appropriate materials. This paper reviews the available methods and materials and their function in MION surface modification as well as their applications for the separation of circulating tumor cells.