Chylous Ascites ; congenital ; therapy ; Humans ; Infant ; Male ; Parenteral Nutrition, Total ; Somatostatin ; therapeutic use

Objective To study the correlation between diabetes and the new discovered angiopietin-related growth factor (AGF)that participates in energy metabolism.MethodsA total of 24 male C.57BL/6 mice were divided into three groups:controlled group; fed with high-fat diet( HFD); fed with high-fat diet for 10 weeks and then treated with rosiglitazone ( HFD + RSG) ; The relative level of AGF mRNA expression in livers was measured by determining a ratio of PCR products of AGF to that of β-actin gene.Oral glucose tolerance test,and insulin resistance index (HOMA-IR) were used to measure blood glucose level and insulin sensitivity.Results Compared with the controls,the expression of AGF mRNA in liver decreased significantly in HFD group( P ＜0.05),and increased significantly in HFD + RSG group( P＜0.01 ).A negative correlation was found between AGF mRNA expression in mice livers and HOMA score ( r =-0.516,P ＜ 0.05 ).Conclusions AGF as a new hepatocyte derived circulating factor counteracts obesity and is related to insulin resistance.

Reflux is a common post-gastrectomy complication due to the destruction of physiological antireflux mechanism,which affects patients' post-surgery life quality greatly.It's one of the basic requirements of digestive tract reconstruction to reduce the incidence of post-surgery reflux,and nowadays there are many kinds of anti-reflux surgical methods,which are also hot topics both at home and abroad.The non-surgical anti-reflux treatments are mainly focused on gastrointestinal motility promotion,mucosa protection,acid suppression,and so on.There are still some controversies concerned with which surgical or non-surgical way to choose.It's of great significance to have a deep insight into the associated mechanisms and make a wise decision on the surgical management with proper non-surgical assistance.

Objective To investigate the neurotoxicity of intrathecal (IT) doxepin in rats.Methods Twenty-four adult male Wistar rats weighing 250-300 g in which intrathecal micro-catheter was successfully implanted without complications were randomly divided into 4 groups ( n =6 each):control group ( group N ) and 3 doxepin groups receiving IT doxepin 40,20 and 10 mmol/L 0.2 μl/g respectively (groups D40,D20,D10 ).In N group normal saline was injected IT instead of doxepin.The animals were killed at 6 h after IT administration.The lumbar segment of the spinal cord was removed for microscopic examination and determination of myelin basic protein (MBP) content in the spinal cord using a double-antibody sandwich (ELISA).Results The severity of neuronal damage and decrease in MBP content in the spinal cord induced by IT doxepin were dose-dependent.Conclusion The neurotoxicity induced by IT doxepin is dose-dependent.

Magnetic iron oxide nanoparticles (MIONs) have been widely used in biomedical fields, including targeted drug deliv-ery, magnetic resonance imaging, separation and enrichment of cancer cells, and tumor-targeted therapy. However, MIONs are increas-ingly necessary to ensure reduced toxicity, more stable colloidal dispersion, better biocompatibility, and higher magnetic responsive-ness. Thus, apart from the continuous improvement of material synthesis, specific surface modification of MIONs is essential to choose appropriate materials. This paper reviews the available methods and materials and their function in MION surface modification as well as their applications for the separation of circulating tumor cells.

The plasmids of recombinant suicide vector containing the truncated stn gene and the deleted stn gene were constructed. The Salmonella stn-deficient mutant was prepared by homologous recombination between the mutant stn gene in the recombinant suicide vector and the wild-type gene in the chromosome of S. Typhimurium 2000. The detection of biological activity of stn gene production in those mutants indicated that Salmonella stn-deficient mutants evoked significantly less fluid secretion in mouse intestinal loops compared to that seen with wild-type Salmonella. Upon oral challenge of mice, the fifty percent lethal dose of the Salmonella stn-deficient mutants was greater than that for the wild-type bacteria. Those studies showed that the stn gene is very important factor in the pathogenesis of salmonellosis.

Objective　To explore the changes and significance of the circulating immunocomplexes(CIC) in patients with chronic iridocyclitis.Methods　We assessed circulating immunocomplexes in peripheral blood of 45 patients with recurrent uveitis and 42 healthy controls by PEG deposition with the biochemical analyzer to obtain levels of CIC different with recurrent uveitis.Results　Levels of CIC were significantly higher in active patients(27.358±6.729)than those in controls(13.459±6.164,P＜0.001) and inactive patients (16.248±6.233,P＜0.001),but patients (inactive) had significantly higher levels than those in controls (P＜0.05).Conclusion　Levels of CIC were found in the groups of active patients with recurrent uveitis (27.358±6.729), but levels of CIC in inactive patients fell. The result of this study suggest that recurrence of uveitis is related to immunocomplexes,and changes in the levels of CIC could be also a measure standard of therapeutic effects.

OBJECTIVE:To study the effects of different extraction methods on the content of cholalic acid from Artificial Bezoar. METHODS: Artificial Bezoar from 3 different habitats was used to extract cholalic acid by hydrolization vs. hydrolization-free method, and the content of cholalic acid was determined by HPLC-ELSD(high performance liquid chromatography with evaporative light scattering detection). RESULTS: The content of cholalic acid in the hydrolyzed sample was higher than that in the non-hydrolyzed sample of the same batch. CONCLUSION: Through hydrolization, the binding cholalic acid in the artificial bezoar was transformed into free cholalic acid so that the content of cholalic acid was increased. Therefore, it is more reasonable to control the quality of Artificial Bezoar by taking the contents of total cholalic acid and free cholalic acid as indexes.

Restenosis is a major complication after percutaneous transluminal coronary intervention. Evaluation of restenosis early and accurately can help to giude treatment, improve outcome and save costs for the patients. All kinds of imaging methods for restenosis have their merits and demerits. It is essential to choose the appropriate imaging method in clinical practice.

Objective To observe the effects of esophageal stricture treated with esophageal stenting.Methods 23 esophagus stenosis patients were expanded to 1 lmm,and the stent was implanted-.Results 23 patients were set into successfully,7 cases could enter the common food,11 caces could enter the soft food,5 cases could enter the semiliquid food.18 cases had the ache,6 cases had the stomach esophagus regurgitation symptom.During 1?12 month follow-up,10 cases survived,13 cases died,the cause of death was mainly later period cancer failure and metastasis.Conclusion Esophagus stenosis treatment operation was simple,safe,and the wound small,the short-term curative effect would be good.