OBJECTIVE:To study the combined antibacterial activity of Rhizoma Coptidis,Bee Propolispro and Fructuts Evodiae against Helicobacter pylori(Hp).METHODS:The protocol was designed by checkboard method.The MICs of Rhizoma Coptidis,Bee Propolispro and Fructuts Evodiae alone or in two-and-two combination against Hp were determined by agar plate method,and the FIC indexes was calculated for the evaluation of the combined effects.RESULTS:When Rhizoma Coptidis combined with Bee Propolispro,the FIC index was 0.16(0.5)when combined with Fructuts Evodiae;The FIC index was 0.05(

OBJECTIVE:To select the best medium prescription of HP.METHOD:To select the best solid medium of HP by orthogonal test design and to study the condition of preserving HP.RESULTS & CONCLUSIONS:There are significant deviations of growing circumstance of HP on the different cultivation medium.The best solid cultivation medium for HP is colombian agar or brain heart infusion agar medium with 2.5%mixed antibiotic added with 5.0%bovine serum and kept the pH of medium at 7.5.HP is preserved under —70 ℃～—80 ℃,which could be successfully antibiotic.

OBJECTIVE: To construct hospital pharmaceutical website and facilitate the evolvement of hospital pharmaceutical care.METHODS: Website management system was developed independently and the system was used to construct hospital pharmaceutical website in our hospital.RESULTS & CONCLUSIONS: Website management system which was designed with B/S three-tier structure which sustains multi-level management authority control and a variety of database and runs stably.The professional website based on website management system is convininent and maintenance,rapid in operation,cheap in cost,low in consumption of human resouces,comprehensive in function,highly professional,and it has greated enhanced the working efficiency in hospital pharmacy.

Objective To investigate and analyze the effect of serum micro inflammatory and nutritional indexes of psychological intervention combined with enalapril maleate tablets on the treatment of the patients with maintenance hemodialysis. Methods 100 patients with chronic renal failure in Jiande the first people's hospital from February 2015 to August 2016 were selected as the subjects, and randomly divided into the control group and the experimental group. The control group and the experimental group were given enalapril maleate tablets, at the same time, the experimental group were received psychological intervention. The changes of serum micro inflammation indexes and nutritional indexes in the the two groups were compared and analyzed. Results The HsCRP, TNF alpha levels in the experimental group decreased significantly than those in the control group, the differences had statistical significant (P<0.05). The levels of prealbumin and plasma albumin in the experimental group were significantly higher than those in the control group (P<0.05). After treatment, the SAS score in the experimental group was (42.3±5.2) points, and the SDS score was (40.1±5.9) points. The SAS score in the control group was (60.9±9.2) points, and the SDS score was (59.1±7.4) points. The scores of depression and anxiety in the experimental group were significantly lower than those in the control group (P<0.05). Conclusion Psychological intervention combined with enalapril maleate tablets used in maintenance hemodialysis patients, which can significantly improve patients' serum inflammation index, restore the nutritional status of patients, eliminate the negative emotion of patients, with further clinical promotion and application significance.

Standardized patients were applied to the inquiry skills training for medical students.As a result,students received systemic training of inquiry contents and techniques earlier,and the capability of communication between doctors and patients was also promoted.Furthermore,SP teaching contributed to students' accurate thinking style focusing on patients and the establishment of their good professional attitude,so it was effective on the course of clinical inquiry skill training.

With the development of conformal radiotherapy,radiotherapy has been used not only in localized prostate cancer as curative therapy.In the treatment of locally advanced prostate cancer,adjuvant therapy after radical prostatectomy and combination therapy with androgen can be used,and can relieve the patients with advanced prostate cancer.The indication,efficiency and side effects of the different techniques are reviewed as follows.

AIM: To investigate the relationship between Alzheimer disease(AD) and alpha 2-macroglobulin(α2M) initially so as to provide evidence for the pathogenesis of late-onset AD.METHODS: The significance of α2M in the late-onset AD was revealed through pathology of α2M in AD and the polymorphism of α2M in AD patients.RESULTS: The association between AD and α2M region on the twelfth chromatosome was analyzed with the genic linkage, and pathological symptom of α2M in AD patients was supported by many evidences. Two polymorphism sites of α2M were located on the 12P12-13, which could increase more risks to AD patients.CONCLUSION: It is significant to investigate the frequency, mutation,polymorphism and expression of gene α2M for the pathogenesis of late-onset AD and its genic diagnosis.

Magnetic resonance imaging (MRI)has apparent dominance in nasopharyngeal carcinoma (NPC) diagnosis,staging,the plan of radiotherapy target and assessment of efficacy because of its high-contrast of parenchyma and muli-parameters imaging.Especially diffusion-weighted imaging (DWI) developed in several years,it can provide qualitative and quantitative information at the cellular level about tumor cellularity and the integrity of cell membranes,which is valuable for tumor detection,cervical lymph nodes metastases,systemic transfer,monitoring and predicting the treatment response of nasopharyngeal carcinoma.

BACKGROUND:Bio-Gide col agen membranes show a good biocompatibility with stem cells. But the research on the osteogenetic differentiation of bone marrow mesenchymal stem cells cultured on the Bio-Gide col agen membranes is rarely reported. OBJECTIVE:To observe the effect of Bio-Gide col agen membranes on the proliferation and the osteogenetic differentiation of bone marrow mesenchymal stem cells. METHODS:Bone marrow mesenchymal stem cells from rabbits were isolated and cultured by using the whole bone marrow adherence method in vitro. Passage 3 bone marrow mesenchymal stem cells were selected and seeded on the Bio-Gide col agen membrane pretreated petri dish (experimental group) and simple petri dish (control group). The proliferation of bone marrow mesenchymal stem cells was detected by cellCounting Kit-8 at 1, 4, 7, 14 days. The supernatant of the cells cultured in osteogenic differentiation medium were col ected to detect the activity of alkaline phosphatase at 1, 4, 7, 14 days. RESULTS AND CONCLUSION:The number of bone marrow mesenchymal stem cells in the two groups was increased with the increasing time, and the control group had more cells than the experimental group at 7 days (P<0.05). There was no significant difference between the two groups at other time points. The alkaline phosphatase activity was increased with the increasing culture time, and the experimental group had a higher activity than the control group at 14 days (P<0.05). There was no significant difference between the two groups at other time points. Experimental findings indicate that Bio-Gide col agen membranes can promote the proliferation and the osteogenetic differentiation of bone marrow mesenchymal stem cells.

Objective To investigate the clinical effects of prostaglandin E1 (PGE1) in the treatment ot chronic renal failure in the 2nd ～ 4th period.Methods 128 patients with CKDs at stage 2 to 4 were selected and randomly divided into the observation group and control group,64 cases in each group.Patients in control group were given routine drug treatment,and patients in observation group were given PGE1 on the basis of the control group.They all treated for 3 months.Renal function indicators before and after treatment,curative effect and adverse reaction were compared.Results There were no statistical differences in renal function indicators before treatment in two groups (t =0.27 ～ 0.95,P ＞ 0.05).After treatment,the 24 hours urinary protein (t =3.41,P ＜ 0.05),creatinine clearance rate (t =4.04,P ＜ 0.05),serum creatinine (t =4.15,P ＜ 0.05),serum urea nitrogen (t =3.92,P ＜ 0.05) between the two groups had statistical differences.Total effective rate of the observation group was 84.37％,which was significantly higher than 57.81％ of the control group (x2 =5.63,P ＜ 0.05).There was no adverse reaction observed in observation group.Conclusion PGE1 might improve patients' proteinuria and renal function in the current situation of lacking effective treatment.