Objective:To investigate the effect of somatostatin (SST) analog octreotide (OCT) in antagonizing the maladjustment of sexual hormones homeostasis induced by tamoxifen(TAM) in rat.Methods:Eight weeks after DMBA(a single dose 100mg/kg body weight by subcutaneous injection) application,the female Wistar rats were randomized to control and treatment groups.Rats in treatment groups underwent TAM(1mg/kg 5 times weekly for 14 weeks by subcutaneous injection),OCT(0.1mg/kg bid for 14 weeks by subcutaneous injection),or the combination of both.The levels of sexual hormones (estradiol,progesterone,prolactin,follicle stimulating hormone,luteinizing hormone) were assessed by enzyme immunoassay(EIA).Results:The levels of estradiol and progesterone in serum were remarkably higher in TAM group(21.69pg/ml,6.14ng/ml) than in control group(16.92pg/ml,4.13ng/ml) respectively(P

Objective To investigate somatostatin receptor subtype 2 (SSTR2) mRNA expression and its correlation with proliferating cell nuclear antigen (PCNA) in human colorectal cancer. Methods A total of 60 colorectal cancer samples were analyzed. The SSTR2 mRNA expression was examined by in situ hybridization (ISH) using multiphase oligoprobes. The PCNA were detected by immunohistochemical staining. A computerized image analysis system was utilized to estimate the relative contents of SSTR2 mRNA. Results The positive rate of expression of SSTR2 mRNA and relative contents in early and well-differentiated colorectal cancer were higher than those in advanced and poorly differentiated lesion. A negative correlation between SSTR2 mRNA expression and PCNA was found in colorectal cancer. Conclusion In the samples of colorectal cancer, the expression of SSTR2 mRNA is correlated with the degree of differentiation and clinical stage, and it plays an important role in reflecting the degree of malignancy and prognosis.

OBJECTIVESTo observe the expression of somatostatin receptor subtype 2 (SSTR2) mRNA, and investigate the relationship between the expression of SSTR2 mRNA and the expressions of estrogen and progesterone receptors (ERs and PRs) in benign and malignant breast tissues.

METHODSSamples from a total of 23 breast carcinomas, 16 mammary hyperplasias, and 9 mammary fibroadenomas were analyzed. SSTR2 mRNA expression was examined by in situ hybridization using multiphase oligoprobes. ER and PR expressions were detected by immunohistochemical staining. A computerized image analysis system was utilized to estimate the relative content of SSTR2 mRNA.

RESULTSThe rate of expression (87.0%) and relative content (0.47) of SSTR2 mRNA in breast cancer were higher than those in benign breast tissue (64%, 0.26) (P < 0.05). SSTR2 mRNA expression was closely correlated with ER and PR expressions in breast cancer (P < 0.05). SSTR2 mRNA was also positively correlated with ER expression in benign breast tissues.

CONCLUSIONSSSTR2 mRNA expression is higher or in benign breast tissues than in malignant ones. There is a significant positive correlation between SSTR2 mRNA and ER and PR expressions. Combined antiestrogen and somatostatin analogue in treatment of ER-positive breast cancers should be further investigated.

Breast Diseases ; metabolism ; Breast Neoplasms ; chemistry ; Humans ; Immunohistochemistry ; In Situ Hybridization ; RNA, Messenger ; analysis ; Receptors, Estrogen ; analysis ; Receptors, Progesterone ; analysis ; Receptors, Somatostatin ; genetics

Objective To research the effect of the identity recognition system based on the Itouch in the patients care .Methods The study group used the identification system of Itouch in all aspects of nursing intervention .Patients without this system were selected as the control group .The rate of operation error , time spent on writing and patient satisfaction rate were compared between groups .Results The error rates of intravenous infusion , oral medicine distribution , specimen collection intravenous injection and skin test were 0.58%,1.42%,0.45%,0.55%,0.62%in the study group, and 0.72%,2.23%,0.79%,0.64%,2.93%in the control group, there were significant differences between two groups (χ2 =5.21,38.37,6.39,7.26,4.85, respectively;P<0.05).The number of patients who were very satisfied , satisfied and dissatisfied was 685,506 and 55 in the study group and 566 , 566 and 146 in the control group , there were significant differences between two groups (χ2 =55.48,P<0.01).The time nurses spent on writing , signature, changing orders and checking were (24 ±12)min,(16 ±3)min,(14 ±5)min in the study group, and (206 ±42)min,(72 ±6)min,(69 ± 17)min in the control group.There were significant differences between two groups (t=22.82,45.72,17.00, respectively;P <0.01).Conclusions The identity recognition system based on the Itouch was helpful to improve the accuracy rate , work efficiency and the satisfaction rate of patients and to reduce the nursing adverse events.

Objective To explore the protective effect in a model of nicotinamide riboside(NR)against carbonyl cyanide m-chlorophenylhydrazone(CCCP)-induced oxidative stress in R28 cells.Methods 4 μmol/L CCCP was used to induce oxidative stress in R28 cells,and 400 nmol/L NR was used to intervene.The cell viability was quantified by CCK-8 assay.The apoptosis was detected by Annexin-V/PI double staining and flow cytometry.Western blotting was used to examine the levels of Cytochrome C,Caspase-3,and Caspase-9 to evaluate the apoptosis.Tetramethylrhodamine ethyl ester was used to detect the mitochondrial membrane potential(MMP),MitoSOX was used to detect the mitochondrial reactive oxygen species(mtROS)levels,and adenosine triphosphate(ATP)assay kit was used to assess ATP generation ability to evaluate mitochondrial function.Results After CCCP treatment of R28 cells,the cell viability decreased,the apoptotic protein levels and apoptosis rates increased,the MMP decreased,and the mtROS generation increased(P＜0.05).After NR pretreatment,the cell viability increased,the apoptotic protein levels and apoptosis rates decreased,the MMP increased,and the mtROS generation decreased(P＜0.05).Conclusion:NR enhances the cell viability,reduces the expression of apoptotic proteins,and ultimately reduces the apoptosis of retinal ganglion cell by inhibiting oxidative stress response and protecting mitochondrial function.