1.Effect of EMS1-siRNA on the proliferation and migrationof human gastric cancer cell line MGC803
Suping HOU ; Xiyan TONG ; Hailong XIE
Chinese Journal of Clinical and Experimental Pathology 2016;32(12):1317-1322
Purpose To explore the effect of EMS1-siRNA on the growth,invasion and migration of human gastric cancer cell line MGC803.Methods It used the Colony formation assay to determine the abilities of proliferation,and flow cytometry analysis to asses cell cycle distribution and apoptosis,transwell invasion and migration experiment to determine the ability of cell invasion and migration after knockdown the expression of EMS1 in MGC803.Results These results suggested that EMS1 gene down-regulated have no affect on cell cycle and cell apoptosis,but the ability of colony formation depressed and migration lowerd obviously (P < 0.05).Conclusion The results shows EMS1 gene is related to proliferation and migration of tumor.
2.The current status and influencing factors of moral distress in clinical nurses
Renshan CUI ; Mei FAN ; Huijuan TONG ; Xiyan ZHANG ; Wei LANG ; Ying LIU
Chinese Journal of Practical Nursing 2014;30(21):64-66
Objective To investigate the current status of moral distress in clinical nurses,and analyze its influential factors.Methods A total of 240 clinical nurses were recruited by stratified sampling method.They were investigated with general situation questionnaire and moral distress scale.Results The average score of moral distress was (2.66±0.41).Multiple linear regression analysis showed that department of work and qualification were influencing factors on moral distress of clinical nurses.Conclusions This study found that nurses' moral distress was above the middle level.Some targeted measures should be taken by nursing managers,in order to help decrease nurses' moral distress.