1.Analysis of the relationship between IL-10 SNPs and pulmonary tuberculosis susceptibility
Shasha WANG ; Liyan YU ; Junling WANG ; Xixia SUN
Journal of Chinese Physician 2015;(z1):41-43,44
Objective To investigate the relationship between IL-10 SNPs and pulmonary tubercu-losis susceptibility.Methods Collected 104 blood samples of pulmonary tuberculosis patients as case group and 104 blood samples of healthy.To analyze genotype SNPs of IL-10-819 -1082 by genomic DNA extraction,PCR amplification and TOF mass spectrometry.Finally the differences of gene and the risk of pulmonary tuberculosis occurrence were analyzed.Results IL-10-819 C/C, C/T and T/T site genotype frequencies were not different between two groups( P >0.05).IL-10-1082 G/G、G/A、A/A site genotype frequencies were not different between two groups( P >0.05).In logistic multivariate regression analysis found that:be compared with IL-10-819 C/C, the risk carrying IL-10-819 C/T, T/T group respectively of pulmonary tuberculosis were OR =0.001(95%CI =0.511~1.921), P =0.977, OR =2.116(95%CI=0.263~1.219), P =0.146, without statistical significance.Be compared with IL-10-1082 A/A, the risk carrying IL-10-1082 G/A、G/G group respectively of pulmonary tuberculosis were OR =0.113(95%CI =0.540~2.393), P =0.737, OR =1.872(95%CI =0.275~1.258), P =0.171, without statisti-cal significance.Conclusions IL-10-819 and -1082 genotype and allele distribution are not different, the mutation of genotype is not correlation with pulmonary tuberculosis.
2.Unexpected Detection of Spontaneous Isolated Superior Mesenteric Artery Dissection by Renal Artery CT Imaging in 12 Hypertension Patients
Shan LIANG ; Zhaoqian WANG ; Chongfu JIA ; Zhiqiang YANG ; Xixia SUN ; Hao WANG ; Xueyan JIA ; Aijun CHEN
Chinese Circulation Journal 2016;31(7):676-678
Objective: To study the features of spontaneous isolated superior mesenteric artery dissection (SISMAD) by unexpected detection of renal artery dual-source CT (DSCT) imaging in hypertension patients. Methods: A total of 4107 patients with suspected secondary hypertension received renal artery DSCT examination in our hospital from 2010-03 to 2015-04 were studied and SISMAD was unexpectedly found in 12 patients. There were 3 patients with mild abdominal pain and the rest without obvious abdominal symptoms. The position and length, true and false lumens, detached tunica intimal flap and branch involvement of dissection, intestinal wall edema and ileus were recorded. Results: SISMAD in all 12 (0.3%) patients were found unexpectedly. Axial CT with post-processing technique clearly displayed the ruptured tunica intimal orifice, true and false lumens, detached intimal flap; the branches were all originated from true lumen. According to Sakamoto classification, all 12 patients were belong to Type I as the true and false lumens were with an entry and re-entry respectively, no filling defect in false lumen. The distance from orifice of dissection to root of abdominal aorta was (26.7 ± 11.3) mm and the length of dissection was (35.1 ± 11.7) mm.There were 10 patients with aneurysmal expansion with the diameter of (11.9 ± 2.5) mm. Conclusion: Unexpected detection of SISMAD by renal artery CT imaging was about 0.3%, radiologist should pay special attention to find superior mesenteric artery dissection in hypertension patients.
3.Natural evolution of pericardial cyst in a 32-year-old male patient.
Pan AN ; Zhaoqian WANG ; Zhiqiang YANG ; Xixia SUN ; Lin LI ; Aijun CHEN ; Chongfu JIA
Chinese Journal of Cardiology 2015;43(7):638-638
Adult
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Humans
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Male
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Mediastinal Cyst
4.Construction and expression of anti-clenbuterol single chain Fv recombinant vector.
Hong WANG ; Yan LIANG ; Jingyi YANG ; Xixia LIU ; Hongbin ZHANG ; Hongtao LEI ; Yudong SHEN ; Yuanming SUN
Chinese Journal of Biotechnology 2008;24(8):1470-1474
To construct the recombinant vector pBV220-scFv and express anti-clenbuterol (CBL) scFv antibody in Escherichia coli, we amplified the scFv gene using plasmid pCANTABSE-CBL as a template, recombined it with pPICZalphaA, then amplified the scFv-His-tag gene from plasmid pPICZalphaA-scFv and linked it with expression plasmid pBV220. We identified the recombinant plasmid by restrictive enzyme digestion, PCR amplification and sequence analysis. Finally, we transformed the recombinant vector into E. coli DH5alpha that was temperature-induced and expressed recombinant protein. We identified the recombinant protein by SDS-PAGE, Western blotting and indirect competitive ELISA. The results show that recombinant plasmid pBV220-scFv contained the inserted fragment with highest homology about 99.8%. The expression of scFv induced by temperature show 37 kD Mw and anti-His-tag mAb recognized-activity by SDS-PAGE and Western blotting respectively, and could competitively combine with CBL, the IC50 is 4.55 ng/mL. The recombinant plasmid pBV220-scFv is constructed and expresses the scFv gene of CBL in E. coli successfully. This study suggests the corresponding immunoassay methods could be set up by the recombinant scFv.
Antibodies
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immunology
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Clenbuterol
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immunology
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Cloning, Molecular
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Genetic Vectors
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genetics
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Immunoglobulin Fragments
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biosynthesis
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genetics
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immunology
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Immunoglobulin Variable Region
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biosynthesis
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genetics
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metabolism
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Recombinant Proteins
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biosynthesis
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genetics
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immunology
5.The value of plaque-to-aorta CT value ratio in differentiating coronary lipid and fibrous plaques
Yaqi GAO ; Xuechang ZHANG ; Yao PAN ; Wei WU ; Zhaoqian WANG ; Xixia SUN ; Shuang PAN ; Hao WANG ; Zhiqiang YANG ; Chongfu JIA
Journal of Practical Radiology 2024;40(2):217-221
Objective To explore the value of plaque-to-aorta CT value ratio(standardized CT value)in differentiating coronary lipid and fibrous plaques,and to preliminarily analyze the stability of the cutoff.Methods Patients who underwent coronary computed tomography angiography(CCTA)and intravascular ultrasound(IVUS)within 1 week were included.The plaque CT value was obtained by measuring the all,four and two short-axis planes,respectively.The CT value of the ascending aorta was measured and standardized(plaque-to-aorta CT value ratio).The receiver operating characteristic(ROC)curves of the standardized and the traditional CT values were drawn.Results A total of 60 patients with 74 plaques were included,35 lipid and 39 fibrous plaques were diagnosed by IVUS.The aorta CT value was significantly correlated with the plaque(r=0.420,P<0.01);the cutoffs for the CT value of all,four and two plaque slices were 55 HU,48 HU and 52 HU,respectively,and all there of the cutoffs of standardized CT value were 0.149;the sensitivity,specificity,positive predictive value(PPV)and negative predictive value(NPV)of four-slice traditional and standardized CT values to differentiate lipid and fibrous plaques were 69%,87%,83%,76%and 91%,82%,82%,91%,respectively.Conclusion Compared with traditional CT value,the standardized CT value can greatly improve the sensitivity and NPV in differentiating coronary lipid and fibrous plaques,while maintaining modest to high specificity and PPV.Furthermore,the cutoff is stable.