Objective To study the preparation and quality control of Shehuang powder.Methods Medicinal materials were toasted,crushed into powder,and mixed in uniformity to make Shehuang powder.TLC was used to identify pronephrium triphyllum and rhubarb in the powder.Results TLC could identify effective reagents.The preparation method was simple and effective.Shehuang Powder prepared with this method was stable.Conclusion The compatibility of Shehuang powder was reasonable.The preparation method and quality Control was simple.

Objective To describe the elderly care needs of older residents living in residential care facilities in Nanchang.Methods Three residential care facilities were purposefully selected and 19 residents were interviewed.The datum were tape-recorded,transcribed in full and then analyzed to identify the main patterns of responses.Results The elderly care needs of older residents in residential care facilities were generated four themes including the body,environment,mind,preparation for death.Conclusions China should establish endowment institutions endowment service needs assessment index system,improve the system of pension agency grading nursing.Enhance communication between pension institutions and medical institutions,to meet the demand for skilled nursing skills in the old man.Carrot and stick,reasonable allocation of resources,physical and social environment of hoisting mechanism,to meet the old man's psychological demand and the demand for environment.Enhancement of the humanistic care of dying old man,to meet the needs of stay in the old man to the hospice.

Objective To study the chemical constituents ofPronephrium triphyllum.Methods The chemical constituents in the plant were isolated and purified with silica gel and Sephadex LH-20.Their structures were identified by analyses of spectral data and physicochemical properties.Results Six compounds were isolated and identified as shelincaoide A(1),n-butyl-13-D-fructopyranoside(2),triphyllin A(3),6,7-di-hydroxycoumarin(4),daucosterol(5),and 13-sitosterol(6),respectively.Conclusion Compound 1 is found to be a new compound.Compounds 2 and 4 are firstly isolated from the plants in Pronephrium Presl.and all compotmds except 3 are obtained from the species for the first time.

OBJECTIVE:To establish the quality criteria for Huangzhi oral liquid.METHODS:TLC was employed to for the qualitative identificaiton of Radix et Rhizoma Rhei,Leech and Fructus Arctii;the content of total nitrogen was determined by KjeLdahL determination and content of Chrysophanol in Radix et Rhizoma Rhei in Huangzhi oral liquids was determined by HPLC.RESULTS:The TLC chromatograms of Radix et Rhizoma Rhei,Leech and Fructus Arctii presented the spots of the same colors with those at the corresponding places in the chromatograms of the controls.There was a good linear relationship within the concentration range of 1.696～ 8.480? g? mL-1 for Chrysophanol(r=0.999 8).The average recovery was 99.60%(RSD=1.37%,n=9).CONCLUSION:The methods are accurate and reliable with a strong specificity and can be used for the quality control of Huangzhi oral liquid.

OBJECTIVE:To evaluate the inhibitory actions of 3 traditional Chinese drugs on human nasopharyngeal carcinoma cells CNE-2 in vitro.METHODS:The IC50(50% inhibiting concentration)of 3 traditional Chinese drugs on human nasopharyngeal carcinoma cells CNE-2 in vitro was measured by MTT assay.RESULTS:The inhibitory actions of 3 traditional Chinese drugs on human nasopharyngeal carcinoma cells CNE-2 in vitro were enhanced with the increase of the concentration in a concentration-dependent manner,with formulation Ⅲ showing the most potent inhibitory action on CNE-2 cells in vitro.CONCLUSION:The heat-clearing and detoxicating traditional Chinese drugs could markedly inhibit the proliferation of CNE-2 cells.

OBJECTIVE:To study the hemostatic,antipruritic,and anti-crissal ulcer effects of Zhishuxi lotion.METHODS:The experimental animals were randomized into four groups(normal control group,Mayinglong-branded musk hemorrhoid ointment,Zhishuxi lotion high-dose group and low-dose group).The time needed to stop bleeding in all groups were tested by cutting mouse tail;the itch-threshold in each group of guinea pigs was determined by inducing itch with histamine phosphate;and the ulceration was observed by causing crissal ulceration in mice with acetic acid.RESULTS:Zhishuxi lotion could shorten the bleeding time,increase the itch threshold in guinea pig,and had some curative effect on acetic acid-induced crissal ulcer in mouse,as compared with the control group.CONCLUSION:Zhishuxi lotion has re-markable effects of hemostasis,itch-relief and anti-crissal ulceration.

OBJECTIVE: To study the preparation and quality control methods of Hewei xiaopi mixture.METHODS: Water extracting-alcohol precipitating was applied for the extraction of majority of drugs and partial drugs were extracted by ethanol extract technology.Then the extractive was mixed to prepare mixture.TLC was used to identify Panax notoginseng,Paevnia lactiflora,Taraxacum mongolicum and Corydalis yanhusuo included in prescription.RESULTS:The preparation was stable in property.P.notoginseng,P.lactiflora,T.mongolicum and C.yanhusuo can be identified by TLC.CONCLUSION: The prescription is characterized with rational compatibility,simple preparation and quality control method,which is suitable for quality control of preparation.

Objective:To investigate the anti-tumor immune responses against renal cell carcinoma induced by dendritic cells(DCs)transfected with survivin gene mediated by recombinant adenovirus.Methods:The DCs derived from human peripheral blood were infected with recombined adenovirus vector carrying the survivin gene.The expression of survivin protein in the infected DCs was examined by Western blotting assay;the surface expression of CD83,MHCⅡ,CD80 and CD86 by flow cytometry(FCM),Interleukin-12(IL-12)in the supernatants of DCs and IFN-? released by the cytotoxic T lymphocytes(CTLs)by ELISA,the ability of DCs in proliferating allo-lymphocytes by mixed lymphocyte reaction(MLR),and specific killing activity of CTLs by MTT assay.Results:The DCs presented mature DCs phenotype after transfection with Ad-svv.The expression of survivin protein in transfected DCs was confirmed by Western blotting analysis.The IL-12 level in the supernatant of DCs transfected with Ad-svv was significantly higher than that transfected with empty vector(Ad-CMV,P

Objective To explore the technique of breast reconstruction after mastectomy. Methods 30 cases of patients with breast cancer who were not suitable for breast conservative surgery were sum-marized. Among them, immediate breast reconstruction with prosthetic implants after mastectomy was used in 16 patients, transverse rectus abdominis musculocutaneous flap (TRAM flap) in 10 patients, and latissimus dorsi musculocutaneous flap in 4 patients. There were 27 patients who underwent immediate breast reconstruction, while 3 patients underwent delayed breast construction. Results The appearance was good in 16 cases of breast reconstruction with prosthetic implants and no complications were found. Two out of the ten patients who had the transevarse rectus abdominis musculocutaneous flap (TRAM flap) breast reconstruction had partly necrosis of skin flap, and one lateral ventral syndrome. The appear-ance of the 7 cases after the TRAM flap breast reconstruction evaluated well, 2 cases moderate, and one case less satisfactory. The appearance of 4 patients who had breast reconstruction with latissimus dorsi musculocutaneous flap evaluated well. Conclusion Breast reconstruction is an important part of combined therapy for the breast cancer, especially for the patients who have strong desire to conserve breast, while incompetence for breast conserving surgery. In such a case breast reconstruction is a good choice. Imme-diate breast reconstruction is better than delayed breast construction. The appropriate method of breast reconstruction depends on each individual patient. Patients with locally advanced breast carcinoma can se-lectively choose immediate breast reconstruction.

OBJECTIVE:To optimize the extraction process for Sihuang paste.METHODS:The Radix et Rhizoma Rhei,Radix Scutellariae were extracted with ethanol,and the effects of the concentration and dosage of ethanol,and the ext-racting time,extraction times on the content of emodin were investigated by orthogonal experiment.The Rhizoma coptidis and Cortex Phellodendri were extracted by water decoction method,and the effects of amount of water,the soaking time,the decoction time and decoction times on the content of tannin were investigated by orthogonal experiment.RESULTS:The optimum alcohol extracting condition for Radix et Rhizoma Rhei,Radix Scutellaria was as follows:adding 60% alcohol at a dose of 6 times that of the total medicinal substances and extracting for 1 h/time for 3 times.The optimum water extracting condition for Rhizoma Coptidis and Cortex Phellodendri was as follows:soaking medicinal substances in water(with the ratio of medicinal substances to water at 1∶6)for 0.5 h,decocting for 2 h and extracting for 3 times.CONCLUSION:The optimized methods are simple and stable,which provide theoretic basis for industrial production.