Objective To investigate the effect of local mild hypothermia on stress hormones in patients with acute cerebral hemorrhage.Methods 78 patients were randomly divided into two groups, mild hypothermia group (38 cases) and routine group (40 cases). Conventional treatment was used in both groups, and local mild hypothermia therapy was applied in mild hypothermia group. The concentrations of corticotropin-releasing hormone (CRH), adrenocorticotropic hormone (ACTH), corticosteroid (CS) and aldosterone (ALD) in plasma pretherapy and at 3rd, 7th day after treatment were measured. The scores of clinical neurological deficit prior and at 3rd week after treatment were observed.Results The levels of plasma ACTH, CRH, CS and ALD prior treatment both in mild hypothermia and routine group were higher than those in healthy control group (all P

Objective To study the angiographic characteristics of coronary heart disease(CHD) in CHD patients with type 2 diabetes mellitus.Methods Analysis was carried out in terms of blood chemistry test result and angiographic data in 454 patients who received selective coronary angiography.Results Compared with the simple CHD patients without type 2 diabetes mellitus,CHD patients with type 2 diabetes mellitus showed higher blood triglyceride(TG) level and lower apoA level.Their coronary angiography showed more four-branch(17.3% vs 9.5%,P

Objective To observe the effects of mild hypothermia on heat shock protein 70 (HSP70) and glial fibrillary acidic protein (GFAP) expression in cerebral tissue after cerebral ischemia and reperfusion. Methods 30 male Wistar rats were randomly divided into sham-operated group, normal temperature group and mild hypothermia group. The model of focal cerebral ischemia was made by middle cerebral artery occlusion (MCAO) method. After 2 h of MCAO following 48 h of reperfusion, HSP70 and GFAP positive neurons were detected by immunohistochemistry technique in ischemic regions of rats. Results Many necrotic neurons were appeared in normal temperature group and no necrotic neurons in sham-operated group. Fewer necrotic neurons were fond in mild hypothermia group than in normal temperature group. The positive cells of HSP70 and GFAP in sham-operated group and mild hypothermia group were less than normal temperature group. Image analysis revealed that the average optical density of HSP70 and GFAP in sham-operated group and mild hypothermia group was significantly lower than in normal temperature group (all P

Objective To investigate the effects of local mild hypothermia therapy on ECG QT dispersion(QTd)and myocardial zymogram in patients with acute cerebral hemorrhage.Methods 70 cases of acute cerebral hemorrhage were divided into local mild hypothermia group(35 cases)and routine treatment group(35 cases)randomly.Routine treatment was used in both groups,and the local mild hypothermia therapy was just applied in local mild hypothermia group.The changes of the ECG QTd and myocardial zymogram were observed before treatment and 1 week after treatment.Results The ECG QTd was shorten and the myocardial enzymes in serum were decreased significantly in both groups at the 1 week after treatment(P

Objective To investigate the effect of folic acid on plasma homocysteine(Hcy) levels and major cardiovascular events in elderly patients with H hypertension.Methods 200 elderly patients with H hypertension were randomly divided into the observation group and control group with 100 cases in each group according to the digital list methods,the control group was given health education and routine anti-hypertensive drug for treatment while the observation group was given folic acid on basis of the conventional treatment,the Hcy levels after 3 months of treatment and incidence of major cardiovascular events among 3 years of the two groups were compared.Results (1) The level of plasma Hcy in the observation group decreased significantly after treatment,with statistically significant difference when compared with before treatment (t =12.32,P ＜ 0.05),that in the control group had no obvious change after treatment than before treatment,there was statistically significant difference between the two groups after treatment(t =11.45,P ＞ 0.05);(2) The occurrence of cardiovascular events among 3 year after treatment of the observation group was 6 cases,the incidence rate was 6％,the control group occurred 15 cases,the incidence rate was 15％,the difference in the incidence of the two groups was statistically significant(x2 =8.24,P ＜ 0.05).Conclusion Folic acid intervention can reduce the plasma Hcy levels in elderly patients with H hypertension and control the occurrence of cardiovascular events.

Objective To investigate the effects of local mild hypothermia treatment on plasma vaso-active substance such as neuropeptide Y(NPY),neurotensin(NT),calcitonin gene-related peptide(CGRP) and endotheline(ET) in patients with acute cerebral hemorrhage. Methods 70 patients were randomly divided into two groups: local mild hypothermia group (35 patients) and routine treatment group (35 patients).Nineteen healthy persons served as control group. Routine treatment was used in the both groups, and the local mild hypothermia therapy was only used in local mild hypothermia group. NPY, NT, CGRP and ET in plasma at the 1 day, 7 days and 14 days after onset were assessed.Results The concentrations of NT, CGRP and ET in the plasma of the two group were markedly higher than control group before treatment (P

Objective To understand the potential risk of schistosomiasis transmission in Xiuzhou District of Jiaxing City, so as to provide the scientific evidence for consolidating schistosomiasis control achievements. Methods Fixed and mobile surveillance sites were set up in Xiuzhou District of Jiaxing City from 2013 to 2015. Oncomelania hupensis snails was surveyed historical snail habitats, current snail habitats, and suspected snail habitats. The schistosome infections were identified using serological and parasitological testing among local residents and mobile populations. In addition, the survival and reproduction of snails imported into Xiuzhou District was observed, and the schistosome infection in wild reservoir hosts was detected. Results A total of 540.14 hm² of settings were surveyed in Xiuzhou District, Jiaxing City from 2013 to 2015, and 1.65 hm² of snail habitats were identified. The snail habitats were mainly located in dry lands, and no infected snails or importation of snails were found. During the period from 2013 to 2015, a total of 7 668 local residents and mobile populations were examined in Xiuzhou District, and no new local infections were detected; however, three imported schistosomiasis cases were identified. Field simulation experiment showed that the imported snails laid eggs and reproduced in Xiuzhou District, and no schistosome infections were found in wild animals. Conclusion There are still residual Oncomelania snails and imported schistosomiasis patients in Xiuzhou District of Jiaxing City; therefore, the surveillance and management of local Oncomelania snails and imported schistosomiasis should be intensified to reduce the risk of schistosomiasis transmission.

OBJECTIVETo investigate the antagonistic effect and mechanism of the effect of cyproheptadine (Cyp) on endotoxic shock in rats.

METHODSEndotoxic shock was produced in rats by i.v. injection of lipopolysaccharides (LPS) (5 mg/kg). Tumor necrosis factor (TNF(alpha)) mRNA expression was assessed by Northern blot. Plasma TNF(alpha) content was measured by radioimmunoassay. Plasma superoxide dismutase (SOD) activity and malondialdehyde (MDA) content were measured. The intracellular free calcium concentration ([Ca(2+)](i)) in single endothelial cells was determined by laser scanning confocal microscopy (LSCM).

RESULTSCyp 5 mg/kg injected immediately after i.v. LPS raised the mean arterial blood pressure (MABP) of shocked rats and improved their 24 h survival rate. Meanwhile, Cyp markedly decreased TNF(alpha) mRNA levels in rat liver (18 +/- 10 vs. LPS + saline 38 +/- 10, P < 0.01) as well as plasma TNF(alpha) content [(7.8 +/- 2.4) microg/L vs. LPS + saline (21.5 +/- 3.2) microg/L, P < 0.01)]. It enhanced plasma SOD activity [(1037.2 +/- 112.8) NU/L vs LPS + saline (615.4 +/- 92.6) NU/L, P < 0.01], reduced the MDA content [(5.2 +/- 1.1) micromol/L vs. LPS + saline (9.8 +/- 1.5) micromol/L, P < 0.01], and inhibited TNF(alpha)-induced [Ca(2+)](i) elevation.

CONCLUSIONCyp exerts an anti-endotoxic shock effect by inhibiting TNF(alpha) gene expression, enhancing SOD activity, reducing lipid peroxidation, and preventing [Ca(2+)](i) overload.

Animals ; Cyproheptadine ; pharmacology ; Histamine H1 Antagonists ; pharmacology ; Malondialdehyde ; blood ; Rats ; Rats, Wistar ; Shock, Septic ; drug therapy ; metabolism ; Superoxide Dismutase ; blood ; Tumor Necrosis Factor-alpha ; biosynthesis ; genetics