BACKGROUND: The stress can induce sympathic nervous-adrenalmedulla system to release a lot of noradrenalin(NE) and adrenalin(E),pro mote bone marrow hemopoietic stem cells to differentiate towards lympho cyte and plasmocyte,decrease the number of granulocyte,thus organism canadapt to surrounding stress,so as to protect critical organs from damage. OBJECTIVE: To observe effect of confinement and vibration stress on in dexes of hematology and myelogram in Chinese tree shrews. DESIGN: Sex-marched and controlled experiment. MATERIALS: Thirty Chinese tree shrews (15 females, 15 males) weigh ing 120 g to 140 g were selected. Self-made experimental cylinders withmany vent-holes, 16 cm length and 6cm inside diameter,were used, one sideof which was sealed with plexglass,and the other side was blocked by mov able plexiglass for animals in and out.The air bath and homothermal vibra tor(Type SHZ-82,made in Changzhou,China). METHODS: The experiment was completed at the Experimental Center ofPublic Security, Gansu Political Science and Law Institute from March toOctober in 2003. ①Thirty Chinese tree shrews were randomly divided intofive groups(n=6):The animals in the control group(C group) were fed nor mally; The experimental groups consisted of reserpine + vibration and vi bration groups. The animals in the reserpine + vibration (R+V)group wereadministered reserpine (0.05 mg/kg, im.) and vibrated at 140 beats/min; the vibration groups (V1,V2,V3) were vibrated at 70, 140, 210 beats/min, respectively. ② The animals in experimental groups were individually keptin the experimental tubes for 5 minutes, then put the tubes into the air bath and homothermal vibrator for 2 minutes. ③ The bleeding time, bloodsedimentation, clotting time, and indexes of hemogram were determinedwith the methods of Duke, Westergren, glass capillary, and full-automaticbiochemical instruments (type 721, Japan), respectively. ④ Dunnett ANO- VA was used to compare the differences between groups . MAIN OUTCOME MEASURES: Comparison of indexes of hematologyand myelogram in Chinese tree shrews betweem the four groups.RESULTS: Thirty Chinese tree shrews were involved in the final analysis.① Indexes of hematology: compared with those of C group, the bleeding time of the V2 group significantly prolonged by 104.8% (P＜0.01 ),but the clotting time and blood sedimentation decreased by 37.3% and 7.5% (P＜0.01 ), respectively. Also, the counts of red blood cells, reticulocyte, blood platelet and white blood cells,as well as content of hemoglobin increased by i7.5% ,50.9%, 11.8%, 10.3% and 13.7% ,respectively.There was no significant difference between R+V group and C group(P ＞0.05),and also in various vibration groups. ②Indexes of myelogram:compared with those of C group,counts of granulocyte were decreased by 31.5%(P＜0.01),counts of red cells,lymphocyte,mononuelear,plasmocyte,megakaryocyte,mitotic cells increased by 25.7% ,77.6%,213.3% ,65.8% ,100%,161.3%,respectively (P＜0.01); those in R+V group were similar to C group (P＞0.05); There were no significant differences among various vibration groups(P＞0.05).CONCLUSION: Confinement and vibration stress can increase significantly the blood cells and reduce counts of granulocyte in bone marrow,and the above changes can be interfered by reserpine.

Aim To study cytotoxic effects and mechanisms of unsaturated fatty acid from mao er yan of gansu origin on BEL-7402 strain cells.Methods The essential components of unsaturated fatty acid from mao er yan of gansu origin were determined by gas chromatography-mass spectrometry and cytotoxic effects of un-saturated fatty acid on BEL-7402 strain cells were determined by using flow cytometry,methods of MTT [3-(4,5-dimethyl thiazol-2yl)-2,5-diphenyltetrazolium bromide] and electron microscope scanning after 48 hours of the unsaturated fatty acid from mao er yan of gansu origin treatment at concentrations of 0.2,0.8 and 3.2 mg?L-1,respectively.Results The unsaturated fatty acid from maoer yan of gansu origin had significantly cytotoxic effects on BEL-7402 strain cells and inhibitory rates for proliferation of BEL-7402 strain cells were 34.6%、44.8% and 70.7% at those three concentrations,respectively.50% inhibiting concentration(IC_ 50)was 0.812 mg?L-1.Apoptosis rates were 18.6%,22.7% and 24.2%,respectively,and occurred cell membrane microvilli breakdown and cell rupture,compared to those of the control group.Conclusion The unsaturated fatty acid from mao er yan of gansu origin has significantly cytotoxic effects on BEL-7402 strain cells and their mechanisms may be related to inhibit cell division,destroy the structure of cell membrane and mitochondria.

Aim To observe the cytotoxic and proliferous effects of Nitraria tangutorum flavone(NTF)on human A-704 strain cells.Methods In vitro cultured human A-704 strain cells,the cytotoxic and proliferous effects of NTF on human A-704 strain cells were determined with methods of trypan-blue and clone formation,and the microelement in cell was detected with X-electron microscope scanning.Results ① The trypan-blue detection showed that the living cells in the NTF group(0.1,0.5 and 5 mg?L-1)and the 5-FU group were decreased by 33.8%~57.3%,35.8%~80.1%,60.8%~99% and 40.3%~59.6% at 6~18 hour after treatment,which significantly differed from those of the control group(P

AIM To study the inhibitory effect and mechanism of n itrary flavone in Hep and U 14 tumor. METHODS The Hep or U 14 tumor cells were injected in the armpit subcutaneous layer of right foreleg of BALB/c strain mice. After 24 h,nitrary flavone at a does of 100 mg?kg -1 ?d -1 body weight or combine nitrary flavone with 5-Fu(100 mg?kg -1 ?d -1 body weight)was given (ip) and inhibitory rate of nitrary flavone for transplanted tumor and the immune function of mice were determined. RESULTS Administration of nitrary flavone to mice for 10 consecutive days exhibited significantly the inhibitory rate for Hep and U 14 by 34 11%,32 14% and 50 73%,47 22%,respectively, as well as the weight of immune organs of mice, formation value of serolysin and phagociytic index were significantly enhanced(P

Objective To explore the value of high frequency ultrasonography for the early diagnosis of hyperuricemia in patients with joint disease.Methods Ninety-eight patients with hyperuricemia and 100 healthy persons,according to with or without history of acute gout attack,were divided into symptomatic group,asymptomatic group and control group,whose first metatarsophalangeal joint,ankle and knee were examined by high frequency ultrasonography.Chi-square test was used for statistical analysis.Results ① The joint lesions detection rate in the symptomatic group,asymptomatic group and the control group was 57％ (13/23),16％ (12/75),0 (0/100) respectively.There was statistical significant difference between the symptomatic group and the asymptomatic group (x2=9.69,P＜0.05).② The symptomatic group had 29 joint involvement (29/138),including 25 at the first metatarsophalangeal joint,3 at the ankle and one at the knee joint.The asymptomatic group had 14 joints involved (14/450),which were all located at the first metatarsophalangeal joint.③ The sonographic appearance of the two groups of arthropathy were synovial thickening,effusion,crystal deposition andbone erosion.The symptoms group presented as tophi,and increased synovial blood flow.Conclusion Patients with asymptomatic hyperuricemia may have joint diseases.The joint disease detection rate of the symptomatic group is higher than that of the asymptomatic group.High frequency ultrasonography can be used a conventional imaging method for the screening of patients with hyperuricemia joint disease.The focus of the screening of asymptomatic patients is the first metatarsophalangeal joint.The focus of the screening of symptomatic patients is the first metatarsophalangeal joint and the joints that were attacked in the past history.

Objective To explore the influence of nursing intervention on the pocket infection and other related problems following CRT-D therapy in chronic heart failure patients.Methods The causes of pocket machinery burst and skin incision dehiscence,pocket bleeding or hematoma and pocket infection following CRT-D therapy from March 2008 to March 2011 were reviewed,then analyzing and summarizing them to work out a detailed nursing interventions plan.During April 2011 to June 2013,nurses carried out the nursing intervention on patients who accepted CRT-D implantation.At last we compared the occurrence of pocket machinery burst and skin incision dehiscence,pocket bleeding or hematoma and pocket infection during the period of 6 months after CRT-D therapy between the two groups.Results After the implementation of nursing interventions,the incidence of pocket infection and other related problems following CRT-D therapy in chronic heart failure patients reduced significantly.Conclusions The interventions such as rigorous preoperative skin preparation,eliminating the potential factors of infection,intraoperative strict aseptic operation,choosing the appropriate position of pocket,the implementation of interventions to prevent bleeding,meticulous and rigorous postoperative nursing,providing healthy knowledge sufciently and strengthening follow-up,finding problems and solving them timely can prevent the pocket machinery burst and skin incision dehiscence,pocket bleeding or hematoma and pocket infection following CRT-D therapy effectively.

[ABSTRACT]OBJECTIVETo study the deposition of suspended particles in the respiratory tract during human breathing.METHODSBased on CT data, three dimensional finite element model of an anatomically accurate respiratory tract, including the nasal cavity, oral cavity, pharynx, larynx, trachea and parts of tracheobronchial tree, is established. Numerical analysis for the deposition of suspended particles in the respiratory tract was conducted. RESULTSThe velocity and pressure distributions of airflow in the respiratory tract were obtained, as well as the particles path and their deposition law.CONCLUSION Respiratory tract plays an important role in filtering suspended particles in the air during breathing. The main deposition sites of the particles were at the front of the inferior turbinate and the posterior pharyngeal wall.

[ABSTRACT]OBJECTIVETo explore the basic characters of the airflow-field in Chinese people's nasal cavity by computational fluid dynamics.METHODSThe three-dimensional, finite-element mesh were developed from Spiral CT imaging scans of nose of the 40 healthy Chinese people. Given the following spatial boundaries of the flow field: no-slip condition was imposed at the surface of the nasal airway walls; a standard atmosphere pressure condition was established at the inlet; a velocity vector was specified at the outlet (nasopharynx), which was obtained under the condition of aspiratory flow rate (12 L/min), the full Navier-Stokes and continuity equations were solved to obtain the airflow pattern.RESULTS1. The airflow passed mainly through left or right side of the nasal airway in the whole 40 cases (left 33, right 7),and the volume of air through the main-side is (320±28) ml while non-main-side (180±45) ml. 2. Airflow velocity: airflow of anterior nostrils, internal nostrils, the middle and inferior parts of the total meatus in the main-side were (5.01±2.12) m/s, (7.00±1.75) m/s, (5.08±1.55) m/s, (4.12±1.40) m/s respectively, and those in non-main-side were (2.01±0.94) m/s, (2.40±0.34) m/s, (1.99±1.0) m/s, (2.01±0.65) m/s respectively, which differences between the both sides were of statistical significance (allP<0.05); airflow of middle meatus, inferior meatus and olfactory cleft in the main-side were (2.08±0.43) m/s, (1.60±0.95) m/s, (1.64±0.80)m/s respectively, and those in non-main-side were (1.89±0.52) m/s, (1.49±0.89) m/s, (1.50±0.75) m/s respectively, which differences were significant statistically (allP>0.05); 3. The airflow form appeared to be linear in the middle and inferior parts of the nasal cavity. 4. Velocity in maxillary sinus cavity was almost 0 m/s.CONCLUSIONThe airflow passes mainly through the middle and inferior parts of the meatus with higher velocity in laminar form and airflow of middle meatus, inferior meatus and olfactory cleft are low and the velocity were slow. Besides, airflow in maxillary sinus cavity diffuses free mainly.

Aim To study the inhibitory effect of Maoeryan vegetable acid on human SGC-7901 strain cells and the mechamism of it. Methods The inhibitory effects of Maoeryan vegetable acid on human SGC-7901 strain cells were determined by flow cytometry, methods of MTT and trypan-blue.Results Maoeryan vegetable acid at a does of 10.0, 1.0 and 0.1 mg?L -1 was given to human SGC-7901 strain cells for 48 h,respectively, and the inhibitory rate of human SGC-7901 strain cells was 82.9%,42.7% and 24.6%,respectively;cellular growth inhibitory rate was 76.4%,56.9% and 42.6%, respectively;as well as apoptosis rate was 26.8%,22.5% and 15.6%, respectively. Conclusion The Maoeryan vegetable acid has significant inhibitory effect on human SGC-7901 strain cells and the inhibitory effect increases with the increase of concentration and time.