Objective To construct a cDNA expression library of psilgramma menephorn and provide the basis for screening the major allergen and producing recombination allergen of psilgramma menephorn. Methods Total RNA was extracted from the whole body of psilgramma menephorn with TRIZOL mRNA purified with Oligo (dT) Spin-Column. ds cDNA was synthesized through reverse transcription. EcoRI adapters were ligated to the blunt ends and the ends were phosphorylated. The XhoI digestion released EcoRI adapter. The fragments smaller than 400 bp were removed by GHROMA SPIN-400 column and the fragments longer than 400 bp were ligated to the Uni-ZAP XR vector. The lambda library was packaged in vitro and is plated on the E.coli cell line XL1-Blue MRF. The content and recombination rate of cDNA library were tested. The length of the inserted fragments was analyzed by PCR. Results The cDNA expression library contained 5?10 5 recombinants and the recombination rate was 67%. The average length of inserted cDNA fragments was about 1.49 kb. Conclusion The constructed cDNA expression library contains appropriate contents and size of cDNA fragments for screening target cDNA to clone.

Computerized intermediate-frequency therapy apparatus is a kind of widely-used clinical equipment. This paper is to indicate the principle and design method of the central control system based on USB for computerized intermediate-frequency therapy apparatus. With this system, the labor intensity, work efficiency and therapeutic efficacy can all be improved.

Objective To investigate the protective and therapeutic effect of large doses of vitamin C on the acute lung injury in the model of rabbits induced by oleic acid. Methods A total of 18 rabbits were divided into four group randomly: control group(CG); oleic acid group(OG); preventive group(PG) and treatment group(TG). Oxygen pressure in arterial blood (PaO_(2)), superoxide dismutase (SOD) and malondialdehyde(MDA) in blood were measured in 0, 30, 90 and 150min, respectively. All the animals were killed in 150min later. Total protein in broncho-alveolar lavage fluid (BALF) and wet dry weight ratio of the right lung were measured, and the left lung sample was treated by formaldehyde for pathological test. Results PaO_(2), SOD in PG group and TG group were significantly higher than that in OG group(P

Objective To study the method of internal boundary parameters identification of middle ear.Method The numerical model is created using CT technology.Based on Matlab tools,the neural network for identifying internal boundary is proposed.Result The uniform pressure of 105 dB is applied at the outside of the tympanic membrane,and the harmonic analysis is calculated on the model to take the training samples.The internal condition parameters are identified using the good neural network.Conclusions The investiga-tion shows that the inverse method reveals a fast convergence and a high degree of accuracy.

A 67-year-old male patient was admitted because of "the right side nasal obstruction repeatedly for 4 years". He got nasal obstruction 4 years ago, especially for the right side nasal cavity, sometimes got blood in his nasal discharge, then the symptom relieved after accepting treatment in local hospital. During the 4 years, the symptom repeatedly occurrence. Three days before hospitalization, the CT examination indicated abnormal things in his nasal cavity and the bone of his nasal sinus had been destroyed. Some abnormal organism were sent to pathological examination, and the report indicated it is acinic cell carcinoma of salivary gland. During the nasal endoscope surgery, a red goiter was found in his nose with its surface crude and brittle. Then we cut the goiter by nasal endoscope, during the operation we find the bottom of the goiter is on the nasal septum. Two weeks after the operation, the patient received the radiation therapy. One year after the operation he doesn't get the abnormal symptom and the nasal MRI not found recidivation.
Aged ; Carcinoma, Acinar Cell ; surgery ; Endoscopy ; Humans ; Magnetic Resonance Imaging ; Male ; Nasal Cavity ; pathology ; Nasal Obstruction ; Nasal Septum ; surgery ; Nasal Surgical Procedures ; Paranasal Sinuses ; pathology ; Salivary Gland Neoplasms ; surgery ; Salivary Glands ; pathology

目的探讨心理支持对焦虑症患者康复的影响。方法60例焦虑症患者随机分为研究组和治疗组各30例,研究组接受药物和心理支持治疗,对照组采用单纯药物治疗,评定两组患者的疗效。结果治疗后,两组患者的焦虑自评量表(SAS)、汉密顿焦虑量表(HAS)评分均明显降低,但研究组的降低幅度明显大于对照组(P<0.01～0.001)。结论心理支持对焦虑症患者的康复有明显的促进作用,近期疗效明显。

Objective To make a bioinformatical analysis of the specific allergen pTSX1 cDNA clone obtained from Humulus pollen in allergic asthma by immunological screening and gene cloning. Methods Bioinformatical approaches, including sequence analysis, sequence alignment, genetic makeup, protein structure and function prediction, were used. Results The analysis of the specific allergen pTSX1 cDNA showed that the gene had no reading frame and coded a protein with 204 amine acids. The analysis of amine acids sequence coded by the pTSX1 cDNA showed that the protein identity of this gene product might be one of ribosomal proteins. Conclusion The pTSX1 cDNA cloned from Humulus pollen in allergic asthma is a new gene, and the protein coded by pTSX1 gene may be one of ribosomal proteins.

Objective To study the effect of specific immunotherapy (SIT) with purified humulus pollen allergens for asthma patients. Methods The humulus pollen allergens were purified partially by gelatin filtration. A double-blinded study was carried out. Eight-six patients with seasonal humulus pollen allergic asthma were divided randomly into two groups: observation group (group A) and control group (group B), which were administered SIT with purified or crude humulus pollen allergens, respectively, before the allergic season for half a year. The clinical effect and side effects were observed. The laboratory observation norms included T lymphocyte subpopulation, the wheals’diameter of IT, the average index of HBDT and the specific IgE before and after SIT . Results The general effect of SIT with purified allergens in group A was 90.7% (39/43), and that with crude allergens in group B was 79.1% (34/43) (P

Objective To construct humulus scandens pollen major allergen DNA vaccine pcDNA-LC2 and evaluate its immunogenicity. Methods Humulus scandens pollen allergen gene was digested from recombinant plasmid pTripIEx2-LC2 by EcoRⅠ and HindⅢ of restriction endonuclease, and then inserted into expression vector pcDNA3.1(-). A large amount of endotoxin pcDNA3.1-LC2 purified plasmid was extracted after successful construction, and animal experiment was carried out to study its immunocompetence. Results Sequencing results showed that 672bp in the coding sequence of frames of recombinant pcDNA3.1-LC2 was in line with the original base line, without deletion or mutation. pcDNA3.1-LC2 of pollen allergen humulus scandens allergen vaccinated BALB/c mice. Double immunodiffusion test showed that pcDNA3.1-LC2 could be expressed and produce antibodies in mice. Conclusion Humulus scandens grass pollen major allergen DNA vaccine pcDNA3.1-LC2 has been successfully constructed. It can be expressed in mice, produces humulus scandens grass pollen allergen specific antibody and has a good immunogenicity.

[ABSTRACT]OBJECTIVETo explore the basic characters of the airflow-field in Chinese people's nasal cavity by computational fluid dynamics.METHODSThe three-dimensional, finite-element mesh were developed from Spiral CT imaging scans of nose of the 40 healthy Chinese people. Given the following spatial boundaries of the flow field: no-slip condition was imposed at the surface of the nasal airway walls; a standard atmosphere pressure condition was established at the inlet; a velocity vector was specified at the outlet (nasopharynx), which was obtained under the condition of aspiratory flow rate (12 L/min), the full Navier-Stokes and continuity equations were solved to obtain the airflow pattern.RESULTS1. The airflow passed mainly through left or right side of the nasal airway in the whole 40 cases (left 33, right 7),and the volume of air through the main-side is (320±28) ml while non-main-side (180±45) ml. 2. Airflow velocity: airflow of anterior nostrils, internal nostrils, the middle and inferior parts of the total meatus in the main-side were (5.01±2.12) m/s, (7.00±1.75) m/s, (5.08±1.55) m/s, (4.12±1.40) m/s respectively, and those in non-main-side were (2.01±0.94) m/s, (2.40±0.34) m/s, (1.99±1.0) m/s, (2.01±0.65) m/s respectively, which differences between the both sides were of statistical significance (allP<0.05); airflow of middle meatus, inferior meatus and olfactory cleft in the main-side were (2.08±0.43) m/s, (1.60±0.95) m/s, (1.64±0.80)m/s respectively, and those in non-main-side were (1.89±0.52) m/s, (1.49±0.89) m/s, (1.50±0.75) m/s respectively, which differences were significant statistically (allP>0.05); 3. The airflow form appeared to be linear in the middle and inferior parts of the nasal cavity. 4. Velocity in maxillary sinus cavity was almost 0 m/s.CONCLUSIONThe airflow passes mainly through the middle and inferior parts of the meatus with higher velocity in laminar form and airflow of middle meatus, inferior meatus and olfactory cleft are low and the velocity were slow. Besides, airflow in maxillary sinus cavity diffuses free mainly.