Objective:To explore the efficacy of abdominal acupuncture in treating patients with osteoporotic vertebral compression fracture (OVCF).
Methods:Forty-five eligible OVCF patients were randomized into an abdominal acupuncture (AA) group, a body acupuncture (BA) group and a medication group according to the random numbers generated by the SPSS 11.5 version software, 15 subjects in each group. Patients in the medication group were intervened by Ossotide injection (intravenous injection), Caltrate (oral administration), Alfacalcidol (oral administration), and Salcatonin injection (muscular injection). In addition to the intervention given to the medication group, patients in the AA group additionally received acupuncture at Zhongwan (CV 12), Xiawan (CV 10), Qihai (CV 6), Guanyuan (CV 4), Shuifen (CV 9), and Huaroumen (ST 24); while patients in the BA group additionally received acupuncture at Jiaji (EX-B 2, superior and inferior to the affected area) points, Ashi points, Weizhong (BL 40) and Chengshan (BL 57). For the AA and BA groups, the acupuncture treatment was given once a day, 6 d per week, for 3 weeks in total. Prior to the intervention, and respectively after 1-week treatment, 2-week treatment and 3-week treatment, visual analogue scale (VAS) and Barthel index (BI) were adopted to evaluate pain degree and activities of daily living (ADL).
Results:After intervention, the VAS and BI scores were significantly changed in the three groups (P<0.05). Respectively after 1-week, 2-week and 3-week treatment, the VAS and BI scores in the two acupuncture groups were significantly different from those in the medication group at the same time point (P<0.01). After 1-week and 2-week treatment, the improvements of VAS and BI scores in the AA group were more significant than those in the BA group(P<0.05); after 3-week treatment, there was no significant difference in comparing the VAS score between the two acupuncture groups (P>0.05), but a significant difference was found in comparing the BI score (P<0.05).
Conclusion:Abdominal acupuncture can produce a more significant effect in improving VAS and BI scores in OVCF patients than body acupuncture and pure medication treatment, and it’s efficient and causes few sufferings.

Objective:To establish the method for determination of bornyl acetate in Kaiweixiaoshi oral liquor by gas chromatography.Methods:Naphthalene has been used as the internal standard substance in internal standard method. The GC system consisted of stainless steel column, PEG 20M as the stationary phase, nitrogen as the carrier gas, and FID as the detector.Results:Both bornyl acetate in the preparation and naphthalenet in internal standard have got satisfactory separation under the chromatographic condition. The mean recovery of bornyl acetate was 95.29%, and RSD was 0.61%.Conclusion: The method is sensitive, accurate and reproducible, and it can be used to control the quality of the preparation.

Adenoviridae ; immunology ; Antigens, Viral ; analysis ; Child ; Fluorescent Antibody Technique ; Humans ; Respiratory Syncytial Viruses ; immunology ; Respiratory Tract Infections ; immunology ; Respirovirus ; immunology ; Virus Diseases ; immunology

To improve the understanding of clinicians on the diagnostic criteria and treatment principlis of pulmonary alveolar proteinosis(PAP),which is a rare respiratory disease.European Respiratory Society published the first edition guidelines for PAP,including a systematic review of the literature and the application of the grading of recommendations,assessment,development and evaluation(GRADE)approach to assess the certainty of evidence and the strength of recommendations.Five questions of patient,intervention,comparison,outcome(PICO)and two narrative questions were developed.Recommendations and evidence-based evidence were given,including management of PAP,whole lung lavage,granulocyte-macrophage colony-stimulating factor(GM-CSF)therapy,rituximab,plasma exchange,and lung transplantation.In addition,recommendations were given for the use of GM-CSF antibody testing,bronchoalveolar lavage,and lung biopsy.This study is to interpret the main content of the guideline.

OBJECTIVETo evaluate the sensitivity and specificity of fully automated immunohistochemistry (IHC), with comparison to FISH, in the detection of EML4-ALK rearrangement in lung adenocarcinoma (ADC); and the use of IHC as a pre-screening tool.

METHODSA total of 404 paraffin-embedded NSCLC samples from surgical resections were tested by IHC with Ventana anti-ALK rabbit monoclonal antibody (D5F3) and ultrasensitive detection kit. ALK rearrangement was further confirmed by FISH.

RESULTSTwenty-nine of 404 lung ADCs (7.2%) were positive for ALK by IHC. ALK positive tumor cells demonstrated strong and diffused granular cytoplasmic staining. All the ALK IHC-positive cases were confirmed to harbor ALK rearrangement by FISH. None of the ALK IHC-negative cases was FISH-positive.

CONCLUSIONSIHC can effectively detect ALK rearrangement in lung cancer. It may provide a reliable and cost-effective diagnostic approach in routine pathologic laboratories for the identification of suitable candidates for ALK-targeted therapy.

Adenocarcinoma ; genetics ; metabolism ; Adult ; Aged ; Aged, 80 and over ; Carcinoma, Non-Small-Cell Lung ; genetics ; metabolism ; Female ; Gene Rearrangement ; Humans ; Immunohistochemistry ; In Situ Hybridization, Fluorescence ; Lung Neoplasms ; genetics ; metabolism ; Male ; Middle Aged ; Oncogene Proteins, Fusion ; genetics ; metabolism ; Sensitivity and Specificity