To examine the protective action and therapeutic effect of anisodamine on acute lung injure and its molecular mechanism,63 rats were randomly divided into three groups :normal control group(NS),lipopolysaccharide (LPS) group and anisodamine (ADM) group.Rat model of acute lung injure(ALI) was produced by injecting LPS(5mg/kg) into the tail vein.The lung index(LI),WBC count,the percentage of polymorphonuclear leukocyte(PMN) in the bronchoalveolar lavage fluid(BALF) were observed at 0,1,3,5 hours(T 0,T 1,T 2,T 3).The concentration of TNF-? were measured both in BALF and plasma at four time points.The expression of P65 was analyzed by immunohistochmical method five hours after injecting LPS.The results showed that LI of ADM group were significantly lower than that of LPS group at T 2 after injecting LPS(P

Objective To understand the distribution of nosocomial infection pathogens and drug - resistance in intensive care unit of our hospital for providing the guidance of clinical rational administration and preventing the hospital infection. Methods Pathogenic bacteria were isolated from the patients who suffered from nosocomial infection in intensive care unit from January of 2008 to December of 2008. They were tested by microbe VITEK and drug - sensitive reagent. Rate of drug resistance of the pathogenic bacteria was analyzed. Results Gram - negative bacilli( G- ) accounted for 47.67% of the isolated pathogenic bacteria,and most were Acinetobacter baumannii (21.65%) , Pscudomonas aeruginosa( 8.00% ) , Stenotropham onasm altophilia(6.33% ) and Klebsiella pneumoniae (4.00%). Grampositive cocci ( G~+ ) accounted for 9.56 % , and most were Staphylococcus aureus (5.56%) and Enterococcus faecium ( 1.44 % ). The rnycetes occupied the 42. 78% of the pathogens. The main pathogenic bacteria were Candida albicans (24. 44% ) and Candida albicans ( 10.89% ). The rate of drug resistance of Gram - negative bacilli( G~- ) was high as a whole,while the rate of mycetes was low. Conclusion Enhance monitoring on pathogenic bacteria distribution and drug resistance analyses of nosocomial infection in ICU could benefit for the guide of clinical rational administration and depressing multidrug - resistant bacteria.