Objective To describe the effect of cannualted rasps in reduc in g the fat embolic risks during preparation for femoral canal in experimental tot al hip arthroplasty. Methods Twelve mongrels were used to perform resection of b ilateral femoral heads, and randomly assigned to one of two groups with six each . In experiment group, impacting cannulated rasp was used in preparing femoral c anal; while, in control group, impacting traditional rasp was used. Both the ras ps were of same shape; however, there was a 4 mm diameter canal inside the cannu lated rasp connecting its two ends. A bone hole was made through the lateral fem oral cortex to the femoral canal at a point 12 cm distal to the greater trochant er in order to measure the intramedullary pressure of femoral canal when the fem oral canal was prepared with either rasp. The pathology of the postmortem pulmon ary tissue was analyzed. Using quantitative morphometry, the size of fat embolus in the lung tissues and the volume proportion of lung tissues occluded by fat w ere measured respectively. The statistical differences between the two groups we re analyzed using t test. Results In control group, the intramedullary pressure during preparation of femoral canal with traditional rasp increased with a peak of (34.5?10.2)kPa, pulmonary fat emboli were found in all mongrels, the volum e proportion of postmortem lung tissue occluded by fat was 0.64%, and pulmonary arterial pressure increased to (2.2?0.4)kPa[the normal value was (0.8?0.3 )kPa]; while in the experiment group, the intramedullary pressure peak increas ed to (22.4?7.7)kPa, significantly lower than that of the control group; the volume proportion of lung tissue occluded by fat was 0.21% which was also signif icantly lower than that of the control group, and pulmonary arterial pressure wa s (1.7?0.4)kPa. The differences between the two groups were significant in al l measured values. Conclusion The present study suggests that conventional prepa ration of canal can cause an increase of intramedullary pressure, which may lead to pulmonary fat embolism and cardiopulmonary impairment. However, the cannulat ed rasp can decrease the volume proportion of lung tissue occluded by fat and al leviate the influence of preparation of canal on cardiopulmonary function.

OBJECTIVE To evaluate the inhibitory effect of HPSE AS-ODN on the invasiveness of human Hep-2 cell lines. METHODS HPSE AS- ODN which was complementary with initiation codon region of HPSE mRNA was designed and synthesized. After embedded by cation lipofectin, it was transfected into Hep-2 cells of human laryngocarcinoma. The expression of HPSE protein and HPSE mRNA in Hep-2 cell lines were detected by flow cytometry and RT- PCR. Meanwhile Matrigel invasive assay was used to measure the inhibitory effect of HPSE AS-ODN on the invasiveness of human Hep-2 cell lines. RESULTS The HPSE protein and HPSE mRNA expression and invasiveness of human Hep-2 cells treated with AS- ODN of different concentrations were significantly decreased as the AS-ODN concentration increasing. There was a significantly difference between control group and each group of AS-ODN respectively (P

ObjectiveTo study the personality traits,interpersonal trust and coping style of juveniles in criminal gangs and their relationship. Methods 15 male juveniles who committed crimes without gangs ( group1 ),52 male juveniles who committed crimes by gangs( group2 ) and 40 male lawful freshmen( group3 ) were tested with Eysenck Personality Questionnaire ( EPQ),Trust Scale and Coping Style Questionnaire.The data were statistically analyzed by covariance analysis or Pearson relation analysis.ResultsAfter the covariant effect of education levels was removed,the levels of P and N in EPQ of group1 ( P:57.49 ± 2.62,N:59.35 ± 3.55 ) and group2 ( P:57.83 ± 1.24,N:59.60 ± 1.68 ) were higher than those of group3 ( P:43.88 ± 4.72,N:39.07 ±6.40) with statistical difference (P＜0.05).The level of E of group1 (63.41 ±3.86) was higher than that of group2 (53.01 ± 1.83 ) with statistical difference (P ＜ 0.05 ).The levels of P,D and F in Trust Scale of greup1 (P:24.68 ± 1.51,D:14.36 ±2.19,F:15.49 ±2.21) and group2(P:22.95 ±0.71,D:22.48 ± 1.04,F:23.09 ±1.05 ) were lower than those of group3 ( P:33.14 ± 2.72,D:40.22 ± 3.95,F:38.44 ± 3.99) with statistical difference (P ＜ 0.01 ).The levels of self-accusation and fantasy in Coping Style Questionnaire of group1 ( 0.80 ±0.08,0.83 ± 0.06 respectively) and group2 (group2:0.59 ± 0.04,0.68 ± 0.03,respectively) were higher than those of group3 (0.39 ± 0.14,0.44 ± 0.11,respectively) with statistical difference (P＜ 0.05 or 0.01 ),while the levels of problem-solving of group1 ( 0.76 ± 0.06 ) and group2 ( 0.70 ± 0.03 ) were lower than that of group3 ( 0.95 ± 0.11 ) with statistical difference (P ＜ 0.05 ).For the male juveniles who committed crimes by gangs,the P of personality traits had a statistical linkage with the problem-resolving and self-accusation of coping style ( r =- 0.389,- 0.395,P ＜ 0.05 ),the N of personality traits had a statiseical linkage with the F of interpersonal trust and the self-accusation of coping style ( r=-0.473,0454,P ＜ 0.05 ),and the E of personality traits had a statistical linkage with the help-seeking of coping style ( r=0.400,P＜ 0.05 ).ConclusionThe personality traits,interpersonal trust and coping styles of male juveniles who committed crimes with or without gangs and the lawful men are different.These psychological factors may have certain effect on juvenile gang crimes.

OBJECTIVE To investigate the expression of tumor necrosis factor receptor associated factor2(TRAF2) and its relationships with apoptosis in thyroid carcinoma.METHODS SP immunohistochemical staining was used to determine the expression of TRAF2 in 41 specimens of thyroid carcinoma tissue(8 follicular carcinoma and 33 papillary carcinoma) and 9 thyroid adenomas tissues.Simultaneously 7 normal thyroid tissue specimens adjacent to the adenomas were taken as the normal control.RESULTS 1.TRAF2 was found in most thyroid tissue specimens,its positive rates(gray value) in normal tissues,adenomas and carcinomas were 47.15 %(125.03?14.91),66.67 %(116.11?12.67) and 80.49 %(105.11?10.9) respectively.Statistic analysis showed TRAF2 expression in thyroid carcinoma tissues was higher compared to that in adenoma and normal tissues(P

ObjectiveTo observe the clinical effect of laparoscopic intrafascial supracervical hysterectomy.Methods The complications during and after the operation of laparoscopic intrafascial supracervical hysterectomy of 46 patients were reviewed and summarized retrospectively. Results Laparoscopic intrafascial supracervical hysterectomy of 46 cases had been done well without significant complications,such as injury of intestine,bladder and ureter.Four cases had complications in short term,one was pelvic hematoma,one was pelvic infection,and two were hematocele in cervical canal.There were five cases had complications in long term.Residual cavity periodic hemorrhage in two cases,cervical gland cyst,pelvic parcel effusion and cervical polypoid hyperplasia each in one case.ConclusionsThere are less complications during the laparoscopic intrafascial supracervical hysterectomy,while much more after the surgery.Therefore,the surgical method needs to be improved.

Objective To investigate the feasibility and effectiveness of ‘ group-head responsibility teaching method' in the physiology experiment teaching.Methods Totally 422 students of clinical medicine major in 2010 grade were randomly divided into two groups:212 students in control and 210 in study group.For students in control group,principles,procedures,items of experiment and computer operation were first taught,then experiment was performed while for those in study group,initial procedures were firstly explained,then the group heads were called together to observe the experiment preformed by teacher,after that the group heads returned to assist other students performing the experiment.Finally,experiment success rates,scores of final-term experiment operation,theoretical exam and report of two groups were compared and analyzed.Meanwhile,questionnaire survey was conducted.Results Success rates were improved significantly in study group than in control group (x2 =37.42,P =0.0000).There were significant differences in operation scores (t =4.3213,P =0.0000),theoretical exam scores (t =6.8744,P =0.0000) and report scores (t =15.298,P =0.0000) between the two groups.Conclusions ‘ Group-head responsibility teaching method' is better than traditional lecture-style teaching method and it can promote comprehensive capabilities of students.

Objective:To examine global expression levels of microRNAs(miRNAs) in mouse cerebrum and to provide an important basis for detailed studies of individual miRNAs,their target genes,the miRNA-related regulatory networks in the mammalian central nervous system,and their implications in diseases.Methods:Low molecular weight RNA from cerebrum of five C57BL/6J mice were tailed and reverse transcribed by extended RT-primer.miRNA primers were carefully designed and arrayed on plates according to the Tm of each primer.PCR was carried out at different annealing temperatures using a gradient real-time PCR instrument.The relative expression level of each miRNA was calculated using 5sRNA for normalization.Results:Among the 285 miRNAs detected,260 were positive with varying abundance.Their frequency distribution was approximately a normal distribution.The expression levels of most miRNAs were in accordance with previously published results by microarray.However,the positive rate was higher than that detected by microarray.miRNAs originating from the same hairpin precursors expressed at similar or significantly different levels.Clusters of proximal miRNAs were similar or quite different in abundance.It is suggested that the fate of miRNA after transcription determined their abundance.Conclusion:Using the RNA-tailing and primer-extension PCR array method,we obtained expression profile of miRNA in mouse cerebrum,especially the relative expression data of many low abundant miRNA in mouse cerebrum,which will be of special help for studying the fine-tuning function of low-level miRNAs.

Objective:To investigate the relationship between different obesity phenotypes and abnormal blood pressure in children and adolescents in Yinchuan city, and to provide appropriate treatment and intervention measures for obese children and adolescents.Methods:The current research design was adopted to facilitate the cluster sampling.A total of 1 047 children and adolescents aged 12 to 18 in Yinchuan were enrolled in this study from September 2017 to September 2018.There were 530 males and 517 females, with an average age of (13.93±1.24) years old.The questionnaire survey, physical examination and laboratory testing were carried out.Statistical analysis was performed by using SPSS 19.0 software.Results:Among the children and adolescents with normal weight, the composition ratio of the metabolically unhealthy normal-weight (MUNW) phenotype was 7.6%.In the obese cases, the composition ratio of the metabolically healthy obesity (MHO) phenotype was 20.2%.The blood pressure of MUNW [systolic pressure SBP: (119±13) mmHg(1 mmHg=0.133 kPa); diastolic pressure(DBP)(74±10) mmHg] and metabolically unhealthy obesity (MUO) [SBP (127±10) mmHg; DBP(74±7) mmHg] phenotypes were significantly higher than those of the metabolically healthy normal-weight (MHNW) phenotype (all P<0.05). The blood pressure of the MUO [SBP(127±10) mmHg; DBP(74±7) mmHg] phenotype was significantly higher than that of the MHO phenotype ( P<0.05). After adjusting for age, gender, and family history of hypertension, MUNW and MUO phenotypes were 5.93 (95% CI: 3.10-11.36) and 11.63 (95% CI: 6.37-21.24) times more likely to develop blood pressure abnormalities than MHNW phenotypes, respectively ( P<0.001). The MHO phenotype was 0.63 (95% CI: 0.08-4.93) times more likely to develop blood pressure abnormalities than the MHNW phenotype ( P=0.66). Conclusions:The MHO phenotype does not increase the risk of abnormal blood pressure, while the MUNW phenotype does.Therefore, it is recommended to identify the MHO phenotype and MUNW phenotype in order to provide appropriate obesity treatment and interventions for children and adolescents.

0.05),and TRAF2 was related to the tumor grade only(P

Objective To study the effect of homeobox genes CDX1 and CDX2 on the phenotype of esophageal adenocarcinoma cells.Methods Esophageal adenocarcinoma cell line SEG-1 was transfected with CDX1 or CDX2 cDNA.The morphology,growth rate,division index and tumorigenicity were analyzed.Results The expression of CDX1 or CDX2 leaded to occurring adeniform-like manifestation.The growth rate,division index and tumorigenicity were reduced,especially by CDX2.Conclusion CDX1 and CDX2 all could increase differentiation of esophageal adenocarcinoma cells and reduction of its malignancy.