OBJECTIVE To evaluate the inhibitory effect of HPSE AS-ODN on the invasiveness of human Hep-2 cell lines. METHODS HPSE AS- ODN which was complementary with initiation codon region of HPSE mRNA was designed and synthesized. After embedded by cation lipofectin, it was transfected into Hep-2 cells of human laryngocarcinoma. The expression of HPSE protein and HPSE mRNA in Hep-2 cell lines were detected by flow cytometry and RT- PCR. Meanwhile Matrigel invasive assay was used to measure the inhibitory effect of HPSE AS-ODN on the invasiveness of human Hep-2 cell lines. RESULTS The HPSE protein and HPSE mRNA expression and invasiveness of human Hep-2 cells treated with AS- ODN of different concentrations were significantly decreased as the AS-ODN concentration increasing. There was a significantly difference between control group and each group of AS-ODN respectively (P

ObjectiveTo study the personality traits,interpersonal trust and coping style of juveniles in criminal gangs and their relationship. Methods 15 male juveniles who committed crimes without gangs ( group1 ),52 male juveniles who committed crimes by gangs( group2 ) and 40 male lawful freshmen( group3 ) were tested with Eysenck Personality Questionnaire ( EPQ),Trust Scale and Coping Style Questionnaire.The data were statistically analyzed by covariance analysis or Pearson relation analysis.ResultsAfter the covariant effect of education levels was removed,the levels of P and N in EPQ of group1 ( P:57.49 ± 2.62,N:59.35 ± 3.55 ) and group2 ( P:57.83 ± 1.24,N:59.60 ± 1.68 ) were higher than those of group3 ( P:43.88 ± 4.72,N:39.07 ±6.40) with statistical difference (P＜0.05).The level of E of group1 (63.41 ±3.86) was higher than that of group2 (53.01 ± 1.83 ) with statistical difference (P ＜ 0.05 ).The levels of P,D and F in Trust Scale of greup1 (P:24.68 ± 1.51,D:14.36 ±2.19,F:15.49 ±2.21) and group2(P:22.95 ±0.71,D:22.48 ± 1.04,F:23.09 ±1.05 ) were lower than those of group3 ( P:33.14 ± 2.72,D:40.22 ± 3.95,F:38.44 ± 3.99) with statistical difference (P ＜ 0.01 ).The levels of self-accusation and fantasy in Coping Style Questionnaire of group1 ( 0.80 ±0.08,0.83 ± 0.06 respectively) and group2 (group2:0.59 ± 0.04,0.68 ± 0.03,respectively) were higher than those of group3 (0.39 ± 0.14,0.44 ± 0.11,respectively) with statistical difference (P＜ 0.05 or 0.01 ),while the levels of problem-solving of group1 ( 0.76 ± 0.06 ) and group2 ( 0.70 ± 0.03 ) were lower than that of group3 ( 0.95 ± 0.11 ) with statistical difference (P ＜ 0.05 ).For the male juveniles who committed crimes by gangs,the P of personality traits had a statistical linkage with the problem-resolving and self-accusation of coping style ( r =- 0.389,- 0.395,P ＜ 0.05 ),the N of personality traits had a statiseical linkage with the F of interpersonal trust and the self-accusation of coping style ( r=-0.473,0454,P ＜ 0.05 ),and the E of personality traits had a statistical linkage with the help-seeking of coping style ( r=0.400,P＜ 0.05 ).ConclusionThe personality traits,interpersonal trust and coping styles of male juveniles who committed crimes with or without gangs and the lawful men are different.These psychological factors may have certain effect on juvenile gang crimes.

Objective To describe the effect of cannualted rasps in reduc in g the fat embolic risks during preparation for femoral canal in experimental tot al hip arthroplasty. Methods Twelve mongrels were used to perform resection of b ilateral femoral heads, and randomly assigned to one of two groups with six each . In experiment group, impacting cannulated rasp was used in preparing femoral c anal; while, in control group, impacting traditional rasp was used. Both the ras ps were of same shape; however, there was a 4 mm diameter canal inside the cannu lated rasp connecting its two ends. A bone hole was made through the lateral fem oral cortex to the femoral canal at a point 12 cm distal to the greater trochant er in order to measure the intramedullary pressure of femoral canal when the fem oral canal was prepared with either rasp. The pathology of the postmortem pulmon ary tissue was analyzed. Using quantitative morphometry, the size of fat embolus in the lung tissues and the volume proportion of lung tissues occluded by fat w ere measured respectively. The statistical differences between the two groups we re analyzed using t test. Results In control group, the intramedullary pressure during preparation of femoral canal with traditional rasp increased with a peak of (34.5?10.2)kPa, pulmonary fat emboli were found in all mongrels, the volum e proportion of postmortem lung tissue occluded by fat was 0.64%, and pulmonary arterial pressure increased to (2.2?0.4)kPa[the normal value was (0.8?0.3 )kPa]; while in the experiment group, the intramedullary pressure peak increas ed to (22.4?7.7)kPa, significantly lower than that of the control group; the volume proportion of lung tissue occluded by fat was 0.21% which was also signif icantly lower than that of the control group, and pulmonary arterial pressure wa s (1.7?0.4)kPa. The differences between the two groups were significant in al l measured values. Conclusion The present study suggests that conventional prepa ration of canal can cause an increase of intramedullary pressure, which may lead to pulmonary fat embolism and cardiopulmonary impairment. However, the cannulat ed rasp can decrease the volume proportion of lung tissue occluded by fat and al leviate the influence of preparation of canal on cardiopulmonary function.

OBJECTIVE To investigate the expression of tumor necrosis factor receptor associated factor2(TRAF2) and its relationships with apoptosis in thyroid carcinoma.METHODS SP immunohistochemical staining was used to determine the expression of TRAF2 in 41 specimens of thyroid carcinoma tissue(8 follicular carcinoma and 33 papillary carcinoma) and 9 thyroid adenomas tissues.Simultaneously 7 normal thyroid tissue specimens adjacent to the adenomas were taken as the normal control.RESULTS 1.TRAF2 was found in most thyroid tissue specimens,its positive rates(gray value) in normal tissues,adenomas and carcinomas were 47.15 %(125.03?14.91),66.67 %(116.11?12.67) and 80.49 %(105.11?10.9) respectively.Statistic analysis showed TRAF2 expression in thyroid carcinoma tissues was higher compared to that in adenoma and normal tissues(P

0.05),and TRAF2 was related to the tumor grade only(P

Objective To study the effect of homeobox genes CDX1 and CDX2 on the phenotype of esophageal adenocarcinoma cells.Methods Esophageal adenocarcinoma cell line SEG-1 was transfected with CDX1 or CDX2 cDNA.The morphology,growth rate,division index and tumorigenicity were analyzed.Results The expression of CDX1 or CDX2 leaded to occurring adeniform-like manifestation.The growth rate,division index and tumorigenicity were reduced,especially by CDX2.Conclusion CDX1 and CDX2 all could increase differentiation of esophageal adenocarcinoma cells and reduction of its malignancy.

Objective:To examine global expression levels of microRNAs(miRNAs) in mouse cerebrum and to provide an important basis for detailed studies of individual miRNAs,their target genes,the miRNA-related regulatory networks in the mammalian central nervous system,and their implications in diseases.Methods:Low molecular weight RNA from cerebrum of five C57BL/6J mice were tailed and reverse transcribed by extended RT-primer.miRNA primers were carefully designed and arrayed on plates according to the Tm of each primer.PCR was carried out at different annealing temperatures using a gradient real-time PCR instrument.The relative expression level of each miRNA was calculated using 5sRNA for normalization.Results:Among the 285 miRNAs detected,260 were positive with varying abundance.Their frequency distribution was approximately a normal distribution.The expression levels of most miRNAs were in accordance with previously published results by microarray.However,the positive rate was higher than that detected by microarray.miRNAs originating from the same hairpin precursors expressed at similar or significantly different levels.Clusters of proximal miRNAs were similar or quite different in abundance.It is suggested that the fate of miRNA after transcription determined their abundance.Conclusion:Using the RNA-tailing and primer-extension PCR array method,we obtained expression profile of miRNA in mouse cerebrum,especially the relative expression data of many low abundant miRNA in mouse cerebrum,which will be of special help for studying the fine-tuning function of low-level miRNAs.

Objective To study the apoptosis mediated by cisplatin on human colorectal tumors (HCT116) cell line and its mechanisms in vitro. Methods The apoptosis levels of HCT116 cells mediated by cisplatin at vari-ous time and in different concentration were measured by M30-ApoptosisTM -ELISA-kits and flow cytometry assay. The expressions of the protein p53,p21 and Bcl-2 were assessed through Western-Blotting. Results Cisplatin in-hibited the proliferation in a time-and dose -dependant manner ( F = 1129. 383, P = 0. 000 and F = 125. 267, P = 0. 000, respectively). The sub-G1 peak detected by flow cytometry at 24,48,72 hours of the apoptosis rates showed a significant difference between the experimental group and the control group (χ2= 5. 669,14.110,12. 221, P = 0. 010,0.003,0. 000,respectively). We found significant differences on the HCT116 cell growth between different time under cisplatin effect (χ2 = 14.008 ,P =0. 003 ). There were significant difference on the CK18-Asp237-Asp396 re-leased by HCT116 cell between different casplatin concentration (F =48. 667 ,P =0.000) as well as different times ( F = 1194. 394, P = 0.000). The Western-Blotting results indicated that the expression level of the p53 ( t = 9.873, -2.906,7. 229,2.776,P =0.000,0. 007,0. 000,0. 011 ) and p21 (t = - 10. 692, - 8. 867, - 15. 063, - 16.281, P = 0. 000,0.001,0.000,0.000 ,respectively) increased gradually, while there are no effect on the expression of the protein Bcl-2(t=1.429,2.011,2.247,2.001,P=0. 178,0.069,0.053,0.062,respectively). Conclusions Cis-platin can induce the apoptosis on HCT116 cells and thus inhibit the reproduction of tumor cells by recovering the function of p.53.

ObjectiveTo observe the clinical effect of laparoscopic intrafascial supracervical hysterectomy.Methods The complications during and after the operation of laparoscopic intrafascial supracervical hysterectomy of 46 patients were reviewed and summarized retrospectively. Results Laparoscopic intrafascial supracervical hysterectomy of 46 cases had been done well without significant complications,such as injury of intestine,bladder and ureter.Four cases had complications in short term,one was pelvic hematoma,one was pelvic infection,and two were hematocele in cervical canal.There were five cases had complications in long term.Residual cavity periodic hemorrhage in two cases,cervical gland cyst,pelvic parcel effusion and cervical polypoid hyperplasia each in one case.ConclusionsThere are less complications during the laparoscopic intrafascial supracervical hysterectomy,while much more after the surgery.Therefore,the surgical method needs to be improved.

OBJECTIVE To investigate the role of XIAP in cisplatin-induced apoptosis and its possible mechanism. METHODS Hep-2 cells were treated with different concentrations of cisplatin for different times. Using crystal violet assay, RT-PCR and flow cytometry (FCM), we investingated the rate of the cell apoptosis and the expression of XIAP protein and mRNA at different times after treating with different concentrations of cisplatin. RESULTS Hep-2 cells were adherent and in normal survival condition with very low apoptosis rate. After treating with cisplatin, the rate of inhibition, the expression of XIAP protein and the rate of apoptosis were remarkably different between different concentrations and times. The expression of XIAP protein was down regulated accompanied by the augmentation of the rate of Hep-2 cell apoptosis. The products of RT-PCR were analyzed, demonstrating that the XIAP mRNA was down regulated with the increased concentrations of cisplatin overtime. Correlation analysis showed the rate of inhibition and the rate of apoptosis were positively correlated with increased concentrations of cisplatin for different times, however, the expression of XIAP protein was negatively correlated. The expression of XIAP protein and the rate of apoptosis were conversely correlated. CONCLUSION The most important pathway that cisplatin induces cell death is apoptosis. The levels of expression of XIAP proteinand mRNA in Hep2 cells are time-and concentration-dependent after treating with cisplatin. Down-regulation of the XIAP protein expression to augment the rate of apoptosis is one of the mechanisms for the cisplatin tokill the carcinoma cells.