Gwe is a new immunological response modifier extracted from a traditional Chinese drug.In our experiments,we demonstrated that:1.Gwe could significantely inhibite micronucleus fre-quencies in bone marrow cells of mice and decrease both the chromosomal aberrations(CA)andsister—chromatid exchange freqencies of splenocytes in mice induced by Cy;2.it could decreasethe activities of IL—6 like substance of splenocytes in mice induced by CY;3.there was a posi-tive correlation between IL—6 activities in the supernatant from cultured spleen cells and mi-cronucleus frequences in bone marrow cells or CA frequences in spleen cells.

Gwe is a new immunological response modifier extracted from traditional Chinese herbs.Inour experiments,We demonstracted that:1.Gwe could significantly inhibite micronucleus fre-quencies in mouse bone marrow cell and decrease both the chromosome aberration (CA) and Sis-ter— chromatid exchang frequencies of splenocytes in mice induced by 1.5Gy X—ray.2.itcould up—regulate the level of IL—1 like substance of splenocytes in mice induced by X—ray.3.there was negetive correlation between the level of IL—1 like substance in the cultured spleencell supernatant and micronucleus frequencies in bone marrow cells or CA frequencies in spleencells.

Objective:To study the possibility that responses of fever and c- Fos expression in rat PVN and NTS to intraperitoneal administration of LPS are rnediated by vagal afferents. Methods: Rectal temperature was detected by digital temperature detecting instrument. c-Fos expressis was detected by immunohistochemistry staining.Results:The rectal temperature change value in vagotomy LPS gnoup was significantlydecreased compared with that in sham IPS group,and there was striking difference betwen them, p ＜0.05.It was increased significantlycompared with that in vagotomy NS group, P ＜ 0.05.The percentage of c-Fos positive neurons in rat PVN and NTS in vagotomy LPS group wassignificantly decreased compared with that in sham LPS group,and there were difference between them, p ＜0.01.Itwas stnkingly increased compared with that in vagotomy conrol group,respectively, P ＜0.01.Conclusion:The results indicate that vagus nerve is one of thepathways of peripheal LPS signal communicating to CNS.

OBJECTIVE To evaluate the effect of AG490 on the invasion of Hep2 laryngeal carcinoma cells, and to investigate the role of STAT3 signaling pathway in human laryngeal carcinoma invasion.METHODS Janus kinase inhibitor AG490 was used to inhibit the activation of STAT3 signaling pathway of Hep2 cells.Hep2 cell invasion was detected by quantitative Matrigel invasion assay.The expression of p-STAT3 was measured by flow cytometry. RESULTS The activation of STAT3 signaling pathway and the invasion of laryngeal carcinomas Hep2 cell line were suppressed by AG490.The expression of p-STAT3 and the invasion cell number of Hep2 cell line in the group treated by AG490 for 24h decreased compared with that in control group(P

Objective:To study the possibility that responses of fever and c Fos expression in rat PVN and NTS to intraperitoneal administration of LPS are mediated by vagal afferents.Methods:Rectal temperature was detected by digital temperature detecting instrument.c Fos expression was detected by immunohistochemistry staining.Results:The rectal temperature change value in vagotomy LPS group was significantly decreased compared with that in sham LPS group,and there was striking difference between them,P

Objective To study the status of helicobacter pylori (Hp) infection and its correlation with gastroesophageal reflux disease(GERD) .Methods The Physical examination and digestive internal medicine outpatients was chosen for the detailed ques-tionnaire and 14C breath test detection of Hp infection ,investigation of GERD prevalence ,analyzed the relations between Hp infec-tion and GERD sick .Results In 220 cases of healthy check-up person ,Hp positive in 108 cases .There were 238 cases of Hp posi-tive in all GERD patients ,the positive rates of Hp infection for 0 - ≤3 months ,3 - ≤6 months and >6 months GERD patients were 47 .8% 、44 .1% and 27 .5% ,respectively .The positive rates of Hp infection for >6 months were significant lower than other groups (P<0 .01) .Health people who check -up with Hp positive were given Hp eradication therapy ,and emphasized the optimi-zation of self -management behavior ,4 weeks after the review ,eradication rate was 100% ,no obvious adverse reaction in the process of taking the medicine .GERD Hp was given conventional acid and increase the stomach power for 2 weeks ,symptoms after joining Hp eradication therapy ,also emphasized the self - management behavior optimization ,without symptoms worsen . Conclusion Hp infection in patients with different degrees at different stages of GERD .Severe symptoms ,repeated visits patients with low Hp infection rate .There is no GERD related cases after Hp eradication in positive health check-up person .

Background and purpose:Proteasome inhibitors constitute a novel class of antitumor agents that has a complex mechanism of action.Previous studies have confirmed that proteasome inhibitor MG-132 can significantly inhibit Hep-2 cell growth and induce cell apoptosis in a manner that is dependent on dosage and time.But it also induced p-STAT3 protein expression.The aim of this study was to explore whether the STAT3 gene can,by transfecting short hair pin RNA(shRNA),enhance the anti-tumor effect of MG-132 on human laryngeal carcinoma cells.Methods:Hep-2 cells were plated into 96-well and 6-well plates and incubated overnight.Then,they were treated with MG-132 alone and combined with pshSTAT3.Their cell growth was detected by MTT assay,and apoptosis was examined with flow cytometry.The protein expression of p-STAT3 was detected by Western blotting.Results:MTT assay showed that a combined group inhibited the proliferation of Hep-2 cells compared to the MG-132 group and pshSTAT3 group(P＜0.01).Flow cytometry showed that apoptosis of the combined group was significantly higher than the MG-132 group and pshSTAT3 group (P＜0.01).Western blotting showed that the p-STAT3 protein expression up-regulation was observed in the MG-132 group,whereas down-regulation was expressed in the combined group and pshSTAT3 group.Conclusion:The shRNA targeting STAT3 gene can prevent the up-regulation of p-STAT3 protein following a MG-132 treatment thereby significantly enhancing the anti-tunlor effect of the protease inhibitor,MG-132,on human laryngeal carcinoma cells.

Purpose　To assess the clinical efficacy and safety of acute cerebral progressive infarction cured with urokinase in combination with heparin sodium. Methods　There were 50 cases in the control group and treated group. The treated group was treated with urokinase 300,000 units/day intravenous for 1～2 days, heparin sodium 12,500 units/day intravenous for 5 days, the control group was treated with heparin sodium 12,500 units/day intravenous for 5 days. All patients were treated within 5 days of onset and the deterioration of neurological deficits within 10 hours. The hypodensity was showed by brain CT scan without hemorrhage, MDS score was given before and after cure 30 days for comparison. Results　The neurological deficits improvement in the treated group was more efficient than the control group and no hemorrhage was found. Conclusion　Middle dose urokinase infusion in combination with heparin sodium intravenous in treatment of acute cerebral progressive infarction was safe and effective and showed obviously clinical valuable.

OBJECTIVE To evaluate the inhibitory effect of HPSE AS-ODN on the invasiveness of human Hep-2 cell lines. METHODS HPSE AS- ODN which was complementary with initiation codon region of HPSE mRNA was designed and synthesized. After embedded by cation lipofectin, it was transfected into Hep-2 cells of human laryngocarcinoma. The expression of HPSE protein and HPSE mRNA in Hep-2 cell lines were detected by flow cytometry and RT- PCR. Meanwhile Matrigel invasive assay was used to measure the inhibitory effect of HPSE AS-ODN on the invasiveness of human Hep-2 cell lines. RESULTS The HPSE protein and HPSE mRNA expression and invasiveness of human Hep-2 cells treated with AS- ODN of different concentrations were significantly decreased as the AS-ODN concentration increasing. There was a significantly difference between control group and each group of AS-ODN respectively (P

Objective To compare effect of chemotherapy agent DDP to MACS in sorting cancer stemcells (CSC) of laryngeal carcinoma cell line Hep-2. Methods CD133 magnetic beads were applied to sort Hep-2 cells. Different dosages of DDP were used to treat Hep-2 cells for 48 hours. Enrichment rate of CD133+ cells by MACS and after DDP treatment was detected by Flow Cytometer (FCM). Morphologic change was observed under inverse-phase microscope. Results FCM showed that the sorting rate of CD133+ cells through MACS was 64.33 %, while after DDP treatment for 48 hours, the rate of CD133+ cells was enriched significantly in each dosage of DDP, with the maximal rate was 50.7 %, in the dosage of 4 μg/ml. There was a significantly difference between MACS and each of DDP group (P <0.01). Cells treated with DDP were abnormal in morphology. Conclusion MACS and DDP sorting has respective advantages in enriching CSC in Hep-2 cell lines.