Objective To develop the technique to detect total core antigen of HCV(Total HCV-cAg) by Enzyme-Linked Immu-nosorbent Assay (ELISA) and apply it for clinical diagnosis. Methods 201 serum samples with anti-HCV antibody were detected total HCV-cAg after pre - treating the samples, then the sensitivity of results were compared with HCV RNA tests. Among them, 176 cases was determined by FQ-PCR, and 25 cases by RT-PCR for HCV-RNA. Results HCV RNA was found in sera from 88 of 201 samples (43.8%). Total HCV-cAg was positive in 71 (35.3%) of 201 samples . There was no significant difference between the detection rate of HCV RNA by PCR and total HCV-cAg by ELISA. Conclusion Detection of total core antigen of HCV is suitable to be used as to diagnose HCV in clinic.

Objective: To investigate the acceptance status of internet+health management among hypertensive patients in Xuzhou city and the related influencing factors. Methods: An interviewing questionnaire survey on the acceptance of internet+health management was conducted among 1,200 hypertensive patients from Xuzhou community health service centers selected by randomized cluster sampling and stratified sampling methods from February tp August 2018, 1 116 valid questionnaires were finally obtained with a response rate of 93.0%. The respondents were divided into two groups according to whether they would accept the internet+health management, and the general conditions and health management status of the two groups were compared. Results: The acceptance rate of internet+health management was 71.9% (802/1 116). The univariate analysis showed that age, education level, occupation, and monthly income of hypertensive patients were associated with the acceptance of internet+health management (P<0.05); patients with family history of hypertension, cardiovascular and cerebrovascular complications and high health literacy had higher acceptance rate of internet+health management (P<0.05). Multivariate analysis showed that educational levels of junior high school and above (OR=0.426, 0.318, 0.089), good sleep (OR=0.453) were protective factors for acceptance of internet+health management; while not knowing the diagnostic criteria for hypertension (OR=1.494), not knowing the blood pressure monitoring (OR=2.668), not knowing ECG monitoring (OR=2.247), quitting smoking (OR=2.444), no complications (OR=2.247), and not participating in community health management (OR=1.758) were the risk factors of acceptance of internet+health management for patients with hypertension. Conclusion The acceptance rate of internet+health management among hypertensive patients in Xuzhou city is relatively high. A variety of factors have impact on the acceptance of internet + health management.