Objective To explore the clinical related prognostic factors of allogeneic hematopoietic stem cell transplantation (allo-HSCT) in treating malignant hematological diseases. Methods From September 1997 to August 2008,a total of 26 patients with hematological diseases were treated with allo-HSCT from HLA identical-sibling and haplo-identical donors in our hospital, including 14 patients with acute leukemias,10 with chronic myeloid leukemias,and 2 myelodysplastic syndromes. Results All patients achieved sustained full donor type engraftment. The cumulative overall survival (OS) was 63.9 %,and cumulative disease free survival (DFS) was 62.6 %. Fifteen patients had graft-versus-host disease (GVHD) (57.7 %),including 8 acute GVHD(aGVHD) (30.8 %) (grade Ⅲ-Ⅳ aGVHD was 15.4 %) and 7 chronic GVHD. GVHD between HLA identical-sibling and haplo-identical donors was different and there was statistic difference between the two groups (P=0.014). 4 patients relapsed,7 patients died. The univariate analysis showed OS were correlated with grade Ⅳ aGVHD (P=0.05) and CMV infection (P=0.027). Conclusion Allo-HSCT is effective for the cure of patients with malignant hematological diseases. The key to improve the efficacy of HSCT is to reduce the incidence of transplant-related complications,especially GVHD and infection.

Objective:To observe the changes of serum adiponectin and uric acid levels in patients with type 2 diabetes mellitus (T2DM) , and to investigate its correlation with diabetic retinopathy (DR) .Methods:Data of 120 hospitalized T2DM patients in our hospital from Jun. 2017 to Jun. 2019 were retrospectively analyzed. According to the fundus findings, they were divided into no DR group (40 cases) , hyperplastic DR group (45 cases) and non-proliferative DR group (35 cases) . 30 healthy cases in the same period were selected as the control group. The level of aboratory biochemical indexes, serum adiponectin and uric acid were compared between the 4 groups. Risk factors affecting DR were analyzed by Logistic multivariate regression.Results:There was no significant difference in TC among the four groups ( P>0.05) . There were significant differences in TG, LDL-C, HDL-C, HbA1c, and FPG among the four groups ( F=1.638, 0.582, 2.715, 5.382, 5.174, P=0.007, 0.016, 0.028, 0.003, 0.001) . TG, HbA1c, and FPG of DR group, non-proliferative DR group and proliferative DR group were significantly higher than those of the control group (no DR group vs the control group: t=2.246, 12.440, 14.539, P=0.028, 0.000, 0.000; Non-hyperplastic DR group vs the control group: t=3.127, 14.775, 14.767, P=0.000; Hyperplastic DR group vs the control group: t=3.349,16.197,12.755, P=0.001,0.000,0.000) , and the level of these indexes were rising with the aggravation of the disease. HDL-C of DR group, non-proliferative DR group and proliferative DR group were significantly lower than that of the control group (non-hyperplastic DR group vs the control group: t=2.113, P=0.040; DR group vs the control group: t=2.778, P=0.007) , and decreased with the aggravation of the disease. There were significant differences in the level of serum adiponectin and uric acid among the four groups ( F=4.728, 26.491, P=0.004, 0.001) . The level of serum adiponectin was significantly lower in no DR group, non-proliferative DR group and proliferative DR group than that in the control group (no DR group vs the control group: t=2.227, P=0.031; Non-hyperplastic DR group vs the control group: t=5.198, P=0.000; Hyperplastic DR group vs the control group: t=6.827, P=0.000) , and decreased with the aggravation of the disease. The level of serum uric acid in the DR group, non-proliferative DR group and proliferative DR group were significantly higher than those in the control group (No DR group vs the control group: t=5.681, P=0.000; Non-hyperplastic DR group vs the control group: t=13.688, P=0.000; Hyperplastic DR group vs the control group: t=23.620, P=0.000) , and increased with the aggravation of the disease. Multivariate Logistic regression analysis showed that the course of diabetes, HbA1c, adiponectin and uric acid were independent risk factors affecting DR. Conclusions:The level of serum adiponectin decreases in DR patients, and the level of serum uric acid increases in DR patients, which relates to DR progress. Adiponectin and uric acid are risk factors affecting DR.

Although oxymatrine (OMT) has been shown to directly inhibit the replication of hepatitis B virus (HBV), limited research has been done with this drug. In the present study, the antiviral effect of OMT was investigated in an immunocompetent mouse model of chronic HBV infection. The infection was achieved by tail vein injection of a large volume of DNA solution. OMT (2.2, 6.7 and 20 mg/kg) was administered by daily intraperitoneal injection for 6 weeks. The efficacy of OMT was evaluated by the levels of HBV DNA, hepatitis B surface antigen (HBsAg), hepatitis B e antigen (HBeAg) and hepatitis B core antigen (HBcAg). The immunoregulatory activity of OMT was evaluated by serum ELISA and flow cytometry. Results shows that OMT at 20 mg/kg inhibited HBV replication, and it was more efficient than entecavir (ETV) in the elimination of serum HBsAg and intrahepatic HBcAg. In addition, OMT accelerated the production of interferon-(IFN-) in a dose-dependent manner in CD4T cells. Our findings demonstrate the beneficial effects of OMT on the enhancement of immunological function and in the control of HBV antigens. The findings suggest this drug to be a good antiviral therapeutic candidate for the treatment of HBV infection.