1.Effects of road transport on hematological and biochemical parameters in new zealand rabbits
Chuanxin YU ; Xiuxiu GAO ; Zicheng WAN ; Wang LIANG ; Xiao WANG
Chinese Journal of Comparative Medicine 2017;27(7):53-58
Objective To observe the effects of road transport on hematological and biochemical parameters in New Zealand rabbits.Methods A total of 12 healthy New Zealand rabbits were selected for 2 h road transport.Blood samples were collected at 0, 24, 48, 72 and 96 h after transport, respectively.White blood cells (WBC), red blood cells (RBC), hemoglobin (HGB), hematocrit (MCV), mean erythrocyte hemoglobin content (MCH), mean erythrocyte hemoglobin concentration (MCHC) and platelets (PLT) were measured using a blood analyzer.Blood alanine aminotransferase (ALT), aspartate aminotransferase (AST), albumin (ALB), total protein (TP), urea nitrogen (UREA), creatinine, uric acid (UA), triglycerides (TG), total cholesterol (COHL), glucose (GLU), hypersensitive C-reactive protein (CRP), α-amylase (AMYL), and creatine kinase (CK) were detected by an automatic biochemical analyzer.Results Compared the parameters before and after transport, The WBC count was increased first (P< 0.05 or P < 0.01) and then decreased after transport, the levels of RBC, HGB, HCT and PLT were decreased first (P< 0.05 or P < 0.01) and then increased after transport, and MCV was significantly high at 96 h after transport (P< 0.05).Among the clinical biochemical parameters, ALT, AST and BUN were firstly elevated (P< 0.05 or P < 0.01) and then decreased.TP, ALB as well as CREA and TG were firstly decreased (P< 0.05 or P < 0.01) and then increased.GLU was significantly low at 24 h after transport (P< 0.05).All parameters except MCV at 96 h after transport were not significantly different from those before transport.Conclusions Changes of blood routine, liver and kidney function indexes, lipid metabolism indexes, glucose metabolism index and creatine kinase index are observed in the New Zealand rabbits after 2-hour road transportation, and all the indicators except MCV return to pre-transport levels within 96 h.
2.Hemodynamic analysis in the fetuses with ductus arteriosus constriction or closure by conventional fetal echocardiography combined with fetal heart quantification technology
Tianjing LI ; Jiancheng HAN ; Yanli HAN ; Ye ZHANG ; Xiaoyan GU ; Shuang GAO ; Xiuxiu HAO ; Yihua HE
Chinese Journal of Ultrasonography 2021;30(3):213-218
Objective:To evaluate the heart hemodynamics in fetuses with premature ductus arteriosus constriction or closure using fetal heart quantification (FHQ).Methods:The clinical data of 50 singleton fetuses with ductus arteriosus constriction ( n=35) or ductus arteriosus closure ( n=15) who underwent echocardiography in Department of Ultrasound, Beijing Anzhen Hospital were retrospectively analyzed, from May 2013 to January 2020. Fifty healthy singleton fetuses were randomly selected as the control group. The ductus arteriosus diameter (DA), pulsatility index (PI), diameter of the left atrium(LA) and right atrium(RA), diameter of the left ventricle (LV) and right ventricle (RV), tricuspid regurgitation/right atrium area ratio (TR/RA Ratio), pressure gradient of tricuspid regurgitation (PG of TR), and heart/chest ratio were measured using conventional fetal echocardiography; the correlations among the parameters were analyzed. Speckle-tracking analysis was used to analysis and compute the LV and RV global spherical index (GSI), fractional area change (FAC) and global strain (GS), the LV ejection fraction(EF) and stroke volume (SV). These variables and their correlations were compared and analyzed. Results:Compared with the control group, the GS and FAC of the LV and RV in the ductus arteriosus constriction or closure groups were lower ( P<0.05) while the LV-SV was higher ( P<0.05). The FAC, GS, and EF values of the LV were higher in the premature ductus arteriosus closure group than in the ductus arteriosus constriction group ( P<0.05), while the RV-FAC was lower ( P<0.05), the RV-GS and LV-SV showed no significant changes ( P>0.05). Correlation analyse showed that the PI was positively correlated with DA( r=0.364, P<0.05); the PG of TR was negatively correlated with DA( r=-0.414, P<0.05); the TR/RA Ratio was negatively linearly correlated with PI( r=-0.388, P<0.05), and positively correlated with RV/LV Ratio ( r=0.369, P<0.05); the other parameters were not significantly correlated with the DA or PI ( P>0.05). Conclusions:Fetal heart hemodynamics in the premature ductus arteriosus constriction or closure groups change significantly, FHQ can provide valuable information for the evaluation of the fetal heart with ductus arteriosus constriction or closure.
3.Microbiological culture of skin lesion exudates and drug sensitivity test in 176 inpatients with pemphigus
Xiuxiu LI ; Nan WU ; Zaixing WANG ; Hui LI ; Shengxiu LIU ; Hongyan WANG ; Min GAO ; Peiguang WANG
Chinese Journal of Dermatology 2020;53(10):791-794
Objective:To analyze results of microbiological culture of lesion exudates and drug sensitivity test in patients with pemphigus, and to optimize the management protocol of pemphigus.Methods:Inpatients with pemphigus were collected from Department of Dermatology and Venereology, the First Affiliated Hospital of Anhui Medical University from January to December in 2008, and the results of microbiological culture of skin lesion exudates and drug sensitivity test were retrospectively analyzed.Results:A total of 176 patients were collected, and skin lesion exudate culture yielded 25 kinds of bacteria and 11 kinds of fungi in 156 (88.64%) patients. Of the 156 patients, 1 kind of pathogen was yielded in 104 (66.67%), and 2 or more kinds of pathogens were yielded in 52 (33.33%). Gram-positive cocci were detected in 134 (76.14%) cases, including Staphylococcus aureus in 80, Staphylococcus hemolyticus in 21, and Staphylococcus epidermidis in 16; Gram-negative bacilli were detected in 66 (37.50%) cases; fungi were detected in 24 (13.64%) cases. In addition, 89 (93.68%) of 95 relapsed patients showed positive culture results, 67 (82.72%) of 81 patients with newly developed pemphigus had positive culture results, and the positive rate significantly differed between the two groups ( χ2 = 5.22, P <0.05). Drug sensitivity test for Staphylococcus aureus isolates showed that 71 (97.26%) of 73 were sensitive to vancomycin, 69 (97.18%) of 71 to linezolid, and 61 (87.14%) of 70 to rifampin. Conclusion:Cutaneous infection, especially Gram-positive cocci infection, is very common in patients with pemphigus, and Staphylococcus aureus clinical isolates were highly sensitive to vancomycin, linezolid and rifampicin.
4. Association between the internal exposure levels of phthalates and executive function of preschool children
Xiuxiu CAI ; Hui GAO ; Shuangqin YAN ; Jie SHENG ; Chunli GU ; Zhongxiu JIN ; Juan QI ; Yuanyuan XU ; Qiufeng ZHANG ; Hui CAO ; Fangbiao TAO ; Jiahu HAO
Chinese Journal of Preventive Medicine 2018;52(3):296-302
Objective:
To investigate the association between the internal exposure levels of phthalic acid esters (PAEs) and executive function (EF) of preschool children.
Methods:
Between October 2008 and October 2010, pregnant women who accepted pregnancy health care services in four municipal medical and health institutions in Ma'anshan city, Anhui Province, were recruited as study objects. A total of 5 084 pregnant women and 4 669 singleton live births were enrolled in this cohort. The follow-up study was conducted from April 2014 to April 2015. A total of 3 725 data-completed preschool children aged 3 to 6 years older entered in this study. The method of analysis seven metabolites of phthalates in urine was high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) and divided objects into low (
5. Pharmacokinetics and tissue distribution of tetrahydropalmatine, nobiletin, and costunolide in rats after oral administration of Dalitong extract
Yuanmao ZHANG ; Ran ZHANG ; Ruiwei LIAO ; Xiuxiu MENG ; Chen XU ; Fang-Fang GAO ; Fang-Fang JIYE ; Guangji WANG ; Lin TAO
Chinese Journal of Clinical Pharmacology and Therapeutics 2024;29(1):37-51
AIM: To investigate the pharmacokinetic properties of the main active components of Dalitong extract in SD rats after oral administration using UPLC-MS / MS. METHODS: An UPLC-MS / MS method was established to simultaneously detect tetrahydropalmatine, nobiletin and costunolide in the plasma and tissues of SD rats. The method was applied to investigate the pharmacokinetic characteristics and tissue distribution. RESULTS: After a single oral administration, the three active components were rapidly absorbed into the body, with a peak concentration (Cmax) of (13.73 ± 7.50), (27.01 ± 17.69) and (6.73 ± 29.94) ng / mL for tetrahydropalmatine, nobiletin, and costunolide, respectively. The time to reach the peak concentration (Tmax) was (1.40 ± 0.93), (0.63 ± 0.28) and (2.38 ± 8.81) h, respectively. The area under the curve (AUC) was (80.43±40.03), (41.30±28.69) and (303.90 ± 136.69) ng · h · mL
6. Cumulative risk assessment of phthalates exposure in preschool children
Hui GAO ; Kui HUANG ; Xiaoyan WU ; Xiuxiu CAI ; Yan HAN ; Peng ZHU ; Jiahu HAO ; Fangbiao TAO
Chinese Journal of Epidemiology 2019;40(5):585-589
Objective:
The urine concentrations of phthalate metabolites were used to estimate the cumulative risk assessment in preschool children in Ma’anshan of Anhui province.
Methods:
Based on the China-Anhui Birth Cohort, the demographic information and urine samples of 3 743 children were collected in Ma’anshan from April 2014 to April 2015. The concentrations of 7 metabolites’ [monomethyl phthalate (MMP), monoethyl phthalate (MEP), monobutyl phthalate (MBP), monobenzyl phthalate (MBzP), mono (2-ethylhexyl) phthalate (MEHP), mono (2-ethyl- 5-oxohexyl) phthalate (MEOHP) and mono (2-ethyl-5-hydroxyhexyl) phthalate (MEHHP)] of 5 phthalates [dimethyl phthalate (DMP), diethyl phthalate (DEP), dibutyl phthalate (DBP), butyl benzyl phthalate (BBzP), and di (2-ethylhexyl) phthalate (DEHP)] in the urine samples of the children were measured by solid-phase extration-triple quadrupole high performance liquid chromatography-tandem mass spectrometry-isotope method. In addition, the estimated daily intakes (EDIs) of 5 phthalates were calculated according to the metabolites’ concentrations. Cumulative risk assessment was performed using hazard quotient (HQ) and hazard index (HI) methods.
Results:
The