Objective To explore the role of serum levels of Klotho protein and fibroblast growth factor(FGF)-23 in patients with chronic kidney disease(CKD).Methods A total of 160 patients with CKD were recruited into CKD group in this study,160 healthy controls were selected in the control group from March 2014 to March 2016.Basic clinical data,blood biochemical index,serum level of Klotho protein and FGF-23 were analyzed and compared between the two groups.Results The hemoglobin,serum albumin,blood calcium,glomerular filtration rate,Klotho protein levels in the CKD group were lower than those in the control group,and the serum creatinine,blood urea,blood serum phosphorus and FGF-23 were higher than those in the control group(P<0.05).With the progress of CKD stages,hemoglobin,glomerular filtration rate,Klotho protein levels gradually decreased,serum creatinine,blood urea,blood serum phosphorus,FGF-23 level increased(P<0.05).The levels of FGF-23 were negatively related to hemoglobin,glomerular filtration rate,Klotho(r=-0.584,0.692,-0.724)and were positively correlated to serum creatinine,blood urea,blood serum phosphorus(r=0.814,0.703,0.527).The levels of Klotho protein were positively related to hemoglobin,glomerular filtration rate(r=0.612,0.685),and were negatively correlated to serum creatinine,blood serum phosphorus,blood urea,FGF-23(r=-0.720,-0.690,-0.519,0.724).Conclusion High concentrations of FGF-23 and Klotho protein with low concentration were not only related to calcium phosphate metabolic disorders of patients with CKD,and were also associated with the prognosis of patients with CKD,which might be early biomarkers and predictor in patients with CKD.

Objective To assess the level and trend of community acquired respiratory distress syndrome (CARDS) toxin after the infection of Mycoplasma pneumonia(MP),and evaluate the clinical characteristics,the level of immunoglobulin E (IgE) and interleukin-4 (IL-4) so as to find the association among these factors.Methods According to whether the child had wheezing symptoms,all the 63 children were divided into wheezing group (26 ca-ses) and non-wheezing group (3 cases).The levels of CARDS toxin were respectively detected in the acute stage of MP infection,3 and 6 months later after MP infection in different groups,moreover,IgE and IL-4 levels were monitored at the same time.Results (1) The mean level of IgE were (384.96 ± 316.62) × 103 IU/L and (87.32 ± 66.32) × 103 IU/L in wheezing group and non-wheezing group,respectively,and there was statistically significant difference (P ＜ 0.05).(2) The load of CARDS toxin in wheezing group and non-wheezing group were (1.87 ± 0.62) Delta Rn and (1.15 ± 0.48) Delta Rn in the stage of acute infection,respectively,and there was statistically significant difference (P ＜ 0.05).Nevertheless,differences between 2 groups after 3 months and 6 months were not significant.(3) In the acute stage,the level of CARDS toxin in the severe cases were higher than the mild cases [(2.37 ± 0.37) Delta Rn vs (1.21 ± 0.45) Delta Rn],and there was statistically significant difference (P ＜ 0.05).(4) IL-4 showed significant difference in the acute stage and 3 months later after acute infection between 2 groups,however,there were no difference between 2 groups after 6 months later.(5)The load of CARDS toxin showed no significant difference between 2 groups at 3 months [(0.96 ± 0.35) vs.(0.99 ± 0.40) Delta Rn,P =0.757] and 6 months [(0.67 ± 0.20) vs.(0.69 ±0.32) Delta Rn,P =0.641] later after MP infection.(6)The children in wheezing group coughed for (24.89 ±7.04) days after acute infection and the last time for non-wheezing group was (16.46 ± 4.79) days,and there was statistically significant difference(P =0.000).Conclusions The load of CARDS toxin decreased after acute MP infection and it was still detectable six months after onset in the blood.The level of CARDS toxin was associated with the cough and wheezing symptom and the severity of disease.