Objective To investigate the clinical treatment of brucellosis spondylitis (BS) through combination of Mongolian medicine and Western medicine.Methods From December 2010 to June 2015,51 patients which were diagnosed BS at the Department of Brucellosis Hulunbuir People's Hospital were enrolled in this study.After injecting etimicin sulfate and levofloxacin for two weeks,rifampin and doxycycline were administrated orally with Silver pearl pill,Pearl pill and Yunxiang pill (tid) for one treatment cycle which contained three courses of each treatment for 3 weeks.Before and after the treatment,the clinical features and imaging examination results were observed of 2 cases and then followed up for one year.Results Before the treatment,any spinal segment can be affected especially the lumbar spine.Two cases of cervical damage,5 cases of thoracic vertebrae,47 cases of lumbar spine,2 cases of sacral vertebrae,and 39 cases of vertebral abscess were found.After treatment,low back pain symptoms disappeared in 10 cases of the patients.Thirty-nine patients with low back pain symptoms and limited movement.Two patients with low back pain aggravated,MRI examination of the lumbar spine showed lesions increased and abscess formation.The effective rate of Mongolian herbal medicine and Western medicine treatment was 96.1％ (49/51).Conclusion The combined treatment of BS through combination of Mongolia medicine and Western medicine is effective.

Objective To investigate the effects of 4 °C hypertonic saline (HTS) on S100 protein in serum and brain tissues of rats after cardiac arrest (SCA). Method Thirty SD male rats were randomly divided into shame-operated group (A), NS group (B) ,4℃ NS group (C), HTS group (D) and 4℃. HTS group (E), in e-qual number ( n = 6). Drugs were given to the rats of all groups at the initiation of CPR except group A. The rat model of CA was induced by asphyxia. Over 24 hours after restoration of spontanous circulation ( ROSC), venous blood sample was drawn to detcect the concentration of serum S100 protein in each group, and the rats were sacrificed and their brain tissues were taken for comparing the expressions of S100 protein in hippocampus. One-way analysis of variance and q -test were used for comparison among groups. P < 0.05 was considered significant. Results Compared with group A, the concentration of serum S100 protein in other groups were much higher ( P < 0.01). Compared with group B,the concentrations of serum S100 protein in groups C, D and E were also much lower ( P < 0.01). Compared with groups D and E, the concentration of serum S100 protein in group C was much higher ( P < 0.01). Compared with group D, the concentration of serum S100 protein in group D was higher ( P < 0.05). Compared with group A, the expressions of S100 protein in rats brain tissues of groups B,C and D were much higher ( P < 0.01). The expression of S100 protein in brain tissue of rats in group E was also higher than that in rats of group A ( P < 0.05). Compared with group B, the expressions of S100 protein in brain tissues of rats in groups C,D and E were lower (P < 0.05 and P < 0.01). Compared with group C, the expressions of S100 protein in brain tissues of rats in groups D and E were lower (P < 0.01). Compared with group D, the expression of S100 protein in brain tissue of rats in group E was lower (P < 0.05). Conclusions After CA the 4℃ HTS can decrease serum S100 protein level and inhibit the expression of S100 protein in hippocampus, then protecting the brain tissue.

96% and with the same bio-activity as unlabeled Huwen toxin-1; Radioactivity detected in epidural space was 38% of injected radioacti vity at 10 min after epidural injection, which demonstrated successful administr ation into epidural space; The maximum serum concentration after epidural or iv administration of [ 125 I]labeled Huwentoxin-1 were determined to be (0 70?0 04) MBq?L -1 and (4 98?0 58) MBq?L -1 , respectively, a t the maximum serum concentration times of 30 min and 2 min. Terminal T 1/2 after epidural or iv administration were (10 36?0 27) h or (11 03?1 16) h, respectively. Cls was (1 29?0 07) L?h -1 ?kg -1 or (1 25? 0 23) L?h -1 ?kg -1 , respectively. Bioavailability after epidural a dministration was(95?5)%. CONCLUSION Concentration-time cur ves of [ 125 I] labeled Huwentoxin-1 after two routes were different. The degradation profiles after epidural and iv injection supported the using of HWTX-1 as analgesic by epidural administration.

Objective Study the epidemiological and clinical findings of patients with Brucellar epididymo-orchitis (BEO),to provide a theoretical basis for clinical diagnosis.Methods A study was carried out to analyze the patients' files of epidemiological,outcome,clinical and laboratory features of 23 cases of BEO and 185 in patients with brucellosis in Hulunbuir People's Hospital during 2011-2013.Results The age of patients with BEO and without epididymo-orchitis (EO) were (34.48 ± 10.32) years old and (42.07 ± 12.19) years old,the difference was statistically significant (t =-2.842,P ＜ 0.05).Patients with BEO and without EO of brucellosis patients with hypogastralgia ratio was 78.3％ (18/23) and 8.6％ (14/162),the difference was statistically significant (x2 =68.238,P ＜ 0.05).The proportion of BEO cases with abnormal White Blood of Cell (WBC) and C-reactive protein (CRP) levels were 34.8％ (8/23) and 91.3％ (21/23),which was significantly higher than those in without EO group [9.9％ (16/162),63.0％ (102/162),x2 =11.066,7.260,all P ＜ 0.05].After 2 to 7 days,BEO patients with scrotal swelling and pain and/or tenderness relieved.After two weeks treatment,11 patients of the urinary system showed normal by ultrasound,and no patients underwent testicular resection.Conclusions Young male patients with Brucellar are prone to BEO.Laboratory abnormalities are mainly leukocytosis and high CRP level.

Objective To analyze clinical and laboratory results of 38 children with brucellosis for providing theoretical basis for its clinical diagnosis and treatment. Methods The epidemiology, clinical features, laboratory characteristics and therapeutic effect of pediatric patients at HulunBuir People's Hospital from January 2011 to December 2015, were retrospectively evaluated. Results Totally there were 38 child patients with brucellosis, 24 males and 14 females, 36 children had directly contacted with animals, and onset seasons were mainly in Summer and Autumn. The dominant manifestations were fever in 33 (86.8%), anorexia in 21 (55.3%), arthralgiaand myalgia in 17 (44.7%), hepatomegaly in 5 (13.2%), splenomegaly in 11 (28.9%), and lymph nodes enlargement in 10 (26.3%). Abnormal laboratory findings included elevated C-reactive protein (CRP) level in 27 (71.1%) and erythrocyte sedimentation rates (ESR) in 24 (63.2%). Both the Rose Bengal test and serum agglutination test (SAT) were positive in 37 patients, only one patient was negative but the blood culture was positive. After at least 12 weeks of combined, adequate and standardized treatment, there were 30 pediatric brucellosis patients cured, improved in 8 cases, the total effective rate was 100.0%. Conclusions In endemic regions, the presence of persistent fever, joint pain and hepatosplenomegaly should alert clinicians the possibility of brucellosis. Early diagnosis and treatment will reduce the incidence of complications.

Objective:To investigate the clinical characteristics of patients infected with Brucella Melitensis ( B. Melitensis) type 1 and type 3 in Hulunbuir, Inner Mongolia Autonomous Region. Methods:A retrospective analysis method was used to collect clinical medical records of patients with brucellosis admitted to Hulunbuir People's Hospital from June 2013 to August 2017, and 71 patients with brucellosis positive in blood culture and identified by polymerase chain reaction (PCR) and AMOS-PCR were selected as the study subjects. According to the identification results, they were divided into B. Melitensis type 1 and type 3 groups. General information, epidemiological characteristics, clinical characteristics, laboratory examinations, complications and efficacy of the two groups were compared. Results:Among 71 patients with brucellosis, 22 cases were B. Melitensis type 1, including 16 males and 6 females, aged (39.91 ± 16.04) years old; 49 cases were B. Melitensis type 3, including 34 males and 15 females, aged (40.67 ± 18.72) years old. There were no significant differences in gender composition and age between the two groups (χ 2 = 0.081, t = 0.166, P > 0.05). There were 10 cases(45.5%) of B. Melitensis type 1 patients living in agricultural areas, 10 cases (45.5%) in pastoral areas, and 2 cases (9.1%) in cities; there were 40 cases (81.6%) of B. Melitensis type 3 patients living in agricultural areas, 7 cases (14.3%) in pastoral areas and 2 cases (4.1%) in cities, and the difference between regions was statistically significant (χ 2 = 9.276, P < 0.05). Testicular swelling and pain symptoms [22.7% (5/22), 6.1% (3/49)] in B. Melitensis type 1 and type 3 patients were compared, the difference was statistically significant (χ 2 = 4.187, P < 0.05); other clinical features were compared, the differences were not statistically significant ( P > 0.05). There were no significant differences in white blood cell count (WBC) and platelet count (PLT) decreased, erythrocyte sedimentation rate (ESR), C-reactive protein (CRP), alanine aminotransferase (ALT) and aspartate aminotransferase (AST) increased between the two groups ( P > 0.05). The number of complications in B. Melitensis type 1 and type 3 patients were 12 cases (54.5%) and 14 cases (28.6%), respectively, and the difference between the two groups was statistically significant (χ 2 = 4.413, P < 0.05). Ten cases (45.5%) of B. Melitensis type 1 patients were cured, 12 cases (54.5%) were improved, 34 cases (69.4%) of B. Melitensis type 3 patients were cured, 15 cases (30.6%) were improved, and there were no invalid or relapsed patients in both groups, the difference in curative effect between the two groups was statistically significant (χ 2 = 3.690, P < 0.05). Conclusions:In Hulunbuir, Inner Mongolia Autonomous Region, there are differences in the living areas of B. Melitensis type 1 and type 3 patients. The B. Melitensis type 1 patients are prone to testicular swelling and pain and brucellosis complications.

Objective To explore the relationship between operation scores and the expression of caveolin-1 ( Cav-1 ) protein in the cortex of mice.Methods Male Kunming mice were used and divided into two groups (excellent group and bad group) according to the training scores after 4 days' Y-maze learning training.The expression of cav-1 protein in cortex and hippocampus of the mice with different scores were measured through Western blot technology.Results Cav-1 protein expression of excellent group( Cav-1/β-actin was 5.71 + 1.11 ) in the cortex was significantly higher than that of the bad group ( Cav-1/β-actin was 1.69 + 0.20) (P ＜ 0.01 ).While there was no significant difference in the two groups in the expression of Cav-1 protein in hippocampus(P ＞ 0.05 ).Conclusion The operation scores have distinct correlation with the expression of Cav-1 protein in the cortex of mice.

Objective To investigate the distribution and proportion of CD8α+α + T cells in lesions and peripheral blood of patients with psoriasis,and to assess their roles in the pathogenesis of psoriasis.Methods An immunofluorescence assay was performed to observe the distribution of CD8α+α+ T cells in lesions of 5 patients with progressive psoriasis vulgaris and normal skin of 5 healthy human controls.Flow cytometry was conducted to determine the proportion of CD8α+α+ T cells,and to measure the expressions of interferon (IFN)-γ and tumor necrosis factor (TNF)-α in peripheral blood from 10 patients with progressive psoriasis vulgaris and 8 healthy human controls.Statistical analysis was carried out by t test with GraphPad Prism software.Results A massive infiltrate mainly composed of CD8α+α+ T cells but not CD8α+β+ T cells was observed in the upper dermis of lesions from the 5 patients with psoriasis,while there was no infiltrate of CD8α+β+ or CD8α+α+ T cells in the normal skin of 5 healthy human controls.Flow cytometry revealed that the proportion of CD8α+α+ T cells was significantly higher in peripheral blood from 10 patients with psoriasis than in that from 8 healthy human controls (26.47％ ± 12.99％ vs.9.12％ ± 4.80％,t =3.96,P＜ 0.001).Significant differences were also noted between the psoriatic patients and healthy human controls in the percentage of cells secreting IFN-γ (47.36％ ± 19.38％ vs.13.44％ ± 9.21％,t =4.54,P ＜ 0.001) and cells secreting TNF-α (54.14％ ± 21.14％ vs.34.03％ ± 17.22％,t =2.17,P ＜ 0.05) in peripheral blood CD8α+α+ T cells.Conclusions Both the distribution and proportion of CD8α+α+ T cells are increased in lesions and peripheral blood from patients with psoriasis,suggesting that CD8α+α+ T cells may be the main subgroup of CD8+ T cells that contribute to the pathogenesis of psoriasis.

Objective To explore the significance of surgical resection for the pineal region tumor,the extent of tumor resection,the surgical approaches and treatment measures of hydrocephalus.Methods Twentythree patients diagnosed of pineal region cancer were recruited for this study.Thirteen patients received ventriculo-peritoneal shunt(V-P),during which 7 cases received intraoperative end-plate colostomy.Two of the 13 cases received a second V-P procedure.Another 13 cases received operation by corpus callosum- septum-Dome Room-the third ventricle approach to remove the tumor; Eight cases underwent the tumor resection by suboccipital supratentorial approach (Poppen approach )and 2 cases underwent the tumor resection by the infratentorial cerebellar approach( Krause approach).Results Total tumor resection was performed in 11 cases,subtotal or major total resection in 8 cases and partial resection in 4 cases.Nine cases underwent postoperative radiotherapy alone,8 receiving radiotherapy plus chemotherapy,and 6 cases receiving neither radiotherapy nor chemotherapy.Two cases died during treatment.Conclusion Though it is not practicable to completely remove the germ cell tumor in patients with relatively large tumors,cerebrospinal fluid circulation pathways should be reestablished,including ventriculo-peritoneal shunt,colostomy from the end of the third ventricle to the end plate.If tumors are not too large,they would be easy to be removed.The effects on the surrounding brain tissue would not be much significant.If the connection of Ⅲ,V ventricles were normal,the patient may not need shunt or colostomy.Radiotherapy and chemotherapy can be given to this kind of patients after the surgery.To improve the efficacy,other types of tumors,expecially the benign tumors,should be totally removed.

Objective: To analyse epidemiological, clinical characteristics and laboratory examination results of 245 occupational brucellosis form 2008 to 2018, which providing theoretical basis for prevention and control of occupational brucellosis. Methods: Based on the China Center for Disease Control and Prevention Information System, a database of occupational brucellosis cases in HunlunBuir from January 2008 to July 2018 was established. The Epidemiological characteristics, Clinical manifestation, laboratory examination of 245 occupational brucellosis and 359 without occupational brucellosis were comparatively analyzed about the same period. Results: Among the 245 patients, 219 were males, 254 in 359, malese were significantly higher than control group (χ²=21.331, P<0.05) . Fever, fatigue, hyperhidrosis and splenomegaly are common in patients with occupational brucellosis and non occupational brucellosis. Arthralgia (54.3%/44.8%) and CRP (81.2%/71.3%) were significantly also higher than control group (χ²=5.193, P<0.05; χ²=7.704, P<0.05) Fever, hyperhidrosis, fatigue and splenomegaly were common clinical manifestation and signs in the two groups. Brucellosis can cause a variety of complications, including osteoarticular hematological system and hepatic involvement, some patients with multiple system damage. Conclusion The incidence of occupational brucellosis in HulunBuir is concentrated in agriculture and animal husbandry. Veterinarians are the main occupational groups, Occupational health interventions should be strengthened for key occupational hazards, regular occupational health examination, avoid chronic brucellosis and protect the health of key occupational groups.