Two models, mouse hot plate and vocalization indused by electrical stimulation of rat's tail were used to demonstrate the antinocice-ptive action of the neurotoxin ( NT ) from cobra venom.The paw-lick latencies of mice of both NT groups in different dose (0.023 mg/kg, 0.03 mg/kg, im ) were significantly prolonged than that of the control group. In the rats, the vocalization threshold of NT group was significantly higher than that of the control group too. This action of NT showed in these models was in good dose-resp-onse relationship. The onset of antinociceptive action of NT was 2 h and reached highe level 3h after intramuscular injection. This effect could be maintained for more than 24 h 3 groups of mice were continuously given normal saline, NT and morphine respectively for 9 days, and no tolerance showed in NT group of mice, but the acute tolerance to morphine occured in morphine group of mice. These result suggested that the analgesic mechanism of NT may be quite different from that fo morphine.

Objective To investigate the expression of interluekin(IL)-18 in BALB/c mice serum and bronchoalveolar fuid infec-ted by mycoplasma pneumonia(MP) and to clarify the relationship between IL-18 and MP infection .Methods A total of 72 BABL/c mice were randomly divided into control group ,MP infection group and treatment group ,each group with 24 mice .MP infection models were established with ripping cultured MP into nasal cavity of mice .Clarithromycin was used at the fifth day in the treat-ment group .The blood of eyeball and bronchoalveolar lavage fluid were collected at the first ,fifth ,ninth day in the three groups ,and detected IL-18 .At the third ,seventh ,forth ,twenty-first ,30th day ,one mice was selected to isolate lung tissue ,and make pathologi-cal section to observe lesion degree .Results Pathological section indicated that lung tissue of mice in the control group had no in-flammatory response ,but lung tissue in the M P infection group had serious inflammatory response ,lung tissue in the treatment group at seventh day had mild inflammatory response .Compared with that of the control group ,the IL-18 in the serum and bron-choalveolar lavage fluid significantly increased at the first ,fifth ,ninth day ,the differences were statistically significant (tfirst day =10 .08 ,tfifth day =9 .14 ,tninth day = 10 .59 ,P< 0 .05) .The IL-18 levels in the serum and bronchoalveolar lavage fluid at fifth day and ninth day in the treatment group were significantly lower than those of the MP infection group(tfifth day =12 .96 ,tninth day =17 .95 ,P<0 .05) .Conclusion MP infection causes IL-18 level increasing ,it is effective to treat with clarithromycin ,and IL-18 might correlate with pneumonia severity .

The excitability of cerebral cortex will change after the onset of ischemic stroke.Repetitive transcranial magnetic stimulation (rTMS) can be used to regulate the excitability in cerebral hemisphere cortex and promote the motor function recovery in patients with ischemic stroke.Studies have shown that both the affected hemisphere low-frequency rTMS and the unaffected hemisphere high-frequency rTMS can promote the motor function recovery in patients with ischemic stroke.The former may be more effective than the

Objective To detect the anti EZH2 autoantibodies IgG expression in hepatocellular carcinoma patient's serum samples and investigate the relationship of anti EZH2 autoantibodies IgG expression with clinicopathological parameters.Methods ELISA method was used to detect the anti EZH2 autoantibodies in serum of 120 cases of HCC patients and compared with 115 healthy controls.Results Serum anti EZH2 antibodies in patients with hepatocellular carcinoma group the content of IgG was 2.015±0.568 μg/ml group,anti EZH2 antibodies IgG content control health was 1.185±0.289 μg/ml.There was significant difference between two groups (t=12.48,P＜0.001).Anti EZH2 antibodies of IgG in serum of pa ticnts with hepatocellular carcinoma was up-regulated and the degree of tumor size,tumor the degree of differentiation,TNM grading and intrahepatic metastasis (t=8.485 ～ 57.93,P＜0.001).And age,sex,drinking,whether HBV infection had no significant difference (t=1.22～5.20,P＞0.05).Conclusion The expression of anti EZH2 autoantibodies in serum was related to the occurrence and development of hepatocellular carcinoma,and the detection of serum anti EZH2 autoantibodies in serum of liver cancer may be helpful to judge the malignant behavior and prognosis of hepatocellular carcinoma (HCC).which may be used as a refcrcnce for the evaluation of the prognosis of HCC patients with IgG antibody against IgG.

Oncosis is another form of cell death, which is different from apoptosis. The review will discuss the recent advances of oncosis on pathological morphology, nuclear biochemical changes and molecular mechanisms. [

Objective To investigate serum anti-insulin-like growth factor-Ⅱ mRNA binding protein1(IMP1) autoantibody IgG expression in hepatocellular carcinoma(HCC) patient and its relationship with clinicopathological parameters.Methods ELISA method was used to detect serum anti-IMP1 autoantibody IgG in 120 cases of HCC,then the detection results were compared those in 115 healthy individuals(control group).The relationship between serum anti-IMP1 autoantibody IgG level with clinicopathological parameters in HCC patients was analyzed.Results The serum anti-IMP1 autoantibody IgG levels in the HCC group and control group were(1.512±0.685),(1.080±0.301)μg/mL respectively,the HCC group was significantly higher than the control group with statistical difference(P<0.001);serum anti-IMP1 autoantibody IgG in HCC patients was related with the tumor size,tumor differentiation degree,TNM grade and intrahepatic metastasis(P<0.001),and had no obvious relation with the age,sex,drinking and HBV infection(P>0.05).Conclusion Serum anti-IMP1 autoantibody IgG expression increased is increased in HCC patient.The detection of serum anti-IgG autoantibody IMP1 level in HCC is conducive to judge the malignancy degree of HCC.

OBJECTIVEThe aim of this study is to analyze the three-dimensional crystal structure of SMU.2055 protein, a putative acetyltransferase from the major caries pathogen Streptococcus mutans (S. mutans). The design and selection of the structure-based small molecule inhibitors are also studied.

METHODSThe three-dimensional crystal structure of SMU.2055 protein was obtained by structural genomics research methods of gene cloning and expression, protein purification with Ni²⁺-chelating affinity chromatography, crystal screening, and X-ray diffraction data collection. An inhibitor virtual model matching with its target protein structure was set up using computer-aided drug design methods, virtual screening and fine docking, and Libdock and Autodock procedures.

RESULTSThe crystal of SMU.2055 protein was obtained, and its three-dimensional crystal structure was analyzed. This crystal was diffracted to a resolution of 0.23 nm. It belongs to orthorhombic space group C222(1), with unit cell parameters of a = 9.20 nm, b = 9.46 nm, and c = 19.39 nm. The asymmetric unit contained four molecules, with a solvent content of 56.7%. Moreover, five small molecule compounds, whose structure matched with that of the target protein in high degree, were designed and selected.

CONCLUSIONProtein crystallography research of S. mutans SMU.2055 helps to understand the structures and functions of proteins from S. mutans at the atomic level. These five compounds may be considered as effective inhibitors to SMU.2055. The virtual model of small molecule inhibitors we built will lay a foundation to the anticaries research based on the crystal structure of proteins.

Bacterial Proteins ; chemistry ; Cloning, Molecular ; Crystallization ; Crystallography, X-Ray ; Dental Caries ; Humans ; Streptococcus mutans ; chemistry ; X-Ray Diffraction

OBJECTIVE:To study the effect of hydrophilic/hydrophobic nano-silica with different adding amount on the stabili-ty of lipo-emulsion. METHODS:Glycyrrhetinic acid lipo-emulsion 4 mL was taken,respectively adding into 0.5%,1.0%,1.5%(m/m,the same below) hydrophilic nSiO2,and 0.4%,0.75,1.0% hydrophobic nSiO2,incubating 2 h in 30 ℃ water;the same batch of Glycyrrhetinic acid lipo-emulsion was treated as blank control. The forms were observed under electron microscopy after treatment,absorbance value was determined,the stability parameter (KE) was calculated according to the absorbance value,then the adding amount of nSiO2 was optimized,3 batches of preparations was prepared,and the verification test was conducted. RE-SULTS:The spherical structure was Glycyrrhetinic acid lipo-emulsion in the electron microscopy,the substance wrapping its sur-face white ring (fully wrapped) or semi-circular structure (not fully wrapped) was nSiO2. KE of hydrophilic nSiO2 were 4.66%, 5.01% and -2.08%,and KE of hydrophobic nSiO2 were 3.02%,4.51% and 7.24%. The optimized adding amount of hydrophilic nSiO2 was 0.2%,0.3% and 0.4%,hydrophobic nSiO2 was 0.1%,0.2% and 0.3%;KE were 6.19%,3.05%,7.84%,8.42%, 2.41%,2.93%,respectively. The optimal adding amount was 0.3% hydrophilic nSiO2 and 0.2% hydrophobic nSiO2;the 3 batches of preparation showed the optimum stability in its own adding amount. CONCLUSIONS:Both hydrophilic and hydrophobic nSiO2 can improve the stability of Glycyrrhetinic acid lipo-emulsion,and preferably 0.3%,0.2%.

Constipation is a major gastrointestinal complication after stroke.Its prevalence is from 22.9％ to 60％.It not only influences the quality of life of patients and restricts their social activities,but also causes poor outcomes.Poststroke constipation is associated with various factors,including the use of multiple drugs,dehydration,and inactivity.Its treatment strategies have based on the experiences and expert opinions.The available evidence suggests that nurse-led intervention may improve defecation process.

Objective To investigate and understand the characteristics and drug intervention of post-traumatic stress symptoms(PTSD) among middle age acute leukemia patients.Methods The Essen Trauma-Inventory (ETI) was administrated to 100 middle age acute leukemia patients who were admitted in our department and study the PSTD symptoms.Results The positive rate of PTSD is 21.21％ in sample of the patients.Total PTSD symptoms scores were (18.10 ± 9.42),there is no significant different between the 3 main PTSD symptoms in these patients(P ＞ 0.05).Type of leukemia,sexuality,levels of education and marital status are influencing factors of PSTD symptoms,there is significant different between the subgroups (P ＜0.01).Drug intervention can significantly relieve PTSD symptoms in these patients (Z =13.56,P ＜ 0.01).Conclusions Middle age acute leukemia patients are generally found PTSD symptoms and with a high incidence rate of PTSD,so it needs timely diagnosis and treatment.