This study was aimed to establish HPLC fingerprint of Chaenomeles speciosa for the place of production and quality control. HPLC was used to comparatively analyze 7 groups of C. speciosa crude drugs from Guizhou Zheng'an and 8 groups of C. speciosa crude drugs from other different production places. The results showed that after the mutual mode of HPLC fingerprint was set up, and the 11 common peaks were pinpointed, comparison of similar degree to crude drugs of other producing areas had been carried on. The main kinds of ingredient in C. speciosa from different places are nearly the same, but the content of each component was different. It was concluded that the method can be used as the identification and quality control of Guizhou Zheng'an C. speciosa, as well as the evaluation of herb quality and standardized planting of GAP, establishment of standard operating procedures of planting and the quality control of C. speciosa.

Objective To research the TLC identification of geniposide in Lian-pu-yin.Methods Gardenia geniposide in Lian-pu-yin of 10 different samples were identified by TLC.Results Geniposide could be detected by TLC.Conclusion This method was simple and accurate.

This study was aimed to establish an HPLC method for the determination of chlorogenic acid, rutin and kaempferide in Solidago decurrens Lour. A Diamonsil C18 column (4.6 mm í 200 mm, 5 μm) was adopted with the mobile phase of acetonitrile-0.2% phosphoric acid. The flow rate was 1.0 mL·min-1. The column temperature was at 25℃. The wavelength of detection was set at 282 nm. The results showed that the calibration curve was linear over the range of 63.2~442.4 μg for chlorogenic acid (r = 0.999 3), 8.1~56.8 μg for rutin (r = 0.999 4) and 10.8~75.7μg for kaempferide (r = 0.999 8). The average recovery of chlorogenic acid was 98.6% (RSD = 1.4%), that of rutin was 99.2% (RSD = 0.8%) and that of kaempferide was 100.3% (RSD = 1.0%). It was concluded that the method was simple, economical and accurate with good reproducibility.

This study was aimed to establish the fingerprints of Qian Solidaginis Herba by HPLC. The fingerprints of Qian Solidaginis Herba were built by using Diamonsil C18 (200 mm í 4.6 mm, 5 μm) column and acetonitrile -0.2%H3PO4 aqueous solution in gradient as mobile phase. The flow rate was 1.0 mL·min-1. Detecting wavelength was set at 282 nm. The total 10 batches of Qian Solidaginis Herba samples and their adulterants were analyzed. The results showed that 13 peaks were identified as the fingerprints of Qian Solidaginis Herba. Under chromatographic conditions mentioned above. It was concluded that the HPLC fingerprints method was found to have satisfactory accuracy, sta-bility and reproducibility, which was a potential method for the quality control of Qian Solidaginis Herba.

The consecutive data of 822 senior public officials in Chengdu undergoing health checkup from 2011 to 2016 were retrospectively reviewed.Among them,56 new cases of diabetes was diagnosed with a cumulative incidence of 6.81％.Fifty six age-and sex-matched healthy subjects served as controls,the risk factors of new-onset diabetes were analyzed with multivariate logistic regression.The results showed that BMI (OR =1.82,95％ CI:1.27-2.59,P =0.00) and fasting plasma glucose (OR =13.63,95％ CI:2.71-68.43,P =0.00) were independent risk factors of new-onset diabetes in senior public officials.

Objective To study the prevalence of ischemic cardiocerebrovascular disease (ICVD) and its risk factors in Chengdu pediatric healthcare people.Methods A total of 822 pediatric healthcare people in our hospital were divided into coronary heart disease (CHD) group (n=43) and CHD-free group (n=779).Their consecutive data from 2011 to 2016 were retrospectively analyzed.The risk factors for ICVD were analyzed by multivariate regression analysis.Results CHD was detected in 523 out of 100 000 cases in 5 years and in 104 out of 100 000 cases in each year.The age was significantly older,the incidence of DM and the serum FBG and Cr levels were significantly higher in CHD group than in CHD-free group (71.26±9.61 vs 63.81±9.79,P=0.000;20.5％ vs 8.2％,P=0.045;6.38±2.48 mmol/L vs 5.09±1.10 mmol/L,P=0.000;80.47± 20.87 μmol/L vs 74.81 ± 15.10 μmol/L,P =0.020).Logistic regression analysis showed that age,smoking,and serum FBG level were closely associated with ICVD (OR=1.104,95％CI:1.057-1.154,P=0.000;OR=2.219,95％CI:1.018-4.835,P=0.045;OR=1.564,95％ CI:1.320-1.852,P =0.000).Conclusion The prevalence of ICVD is relatively low in pediatric healthcare people,which is closely associated with age,smoking and hyperglycemia.