Objective:To investigate the culture technique and secretory function of parathyroid cells in rats in order to establish experimental basis of parathyroid cell transplantation.Methods:After parathyroid glands were digested by collagenase and trypsin,isolated parathyroid cells were cultured.The cells cultured for 5 days were observed by transmission electron microscopy.Parathyroid hormone obtained from the cell culture fluid on day 1,3,5 and 7 was measured respectively.Results:Parathyroid cells cultured for 5 days had abundant rough surfaced endoplasmic reticula、Golgi bodies、stab mitochondria and secretory granules.Cultured parathyroid cells could secrete parathyroid hormone normally,especially on the 5th day.Conclusion:Parathyroid cells cultured for 5 days still possess original morphologic structure and have maximum secreting function,so it is an optimal donor for transplantation.

The research of the secondary damage remote from middle cerebral artery territory infarction has made significant progress in recent years.More animal experiments from the cellular,biochemical and molecular levels have been performed for in-depth and detailed research on remote site damage. The injury mechanisms such as oxidative damage and β amyloid deposition have been found.The new imaging detection technologies,such as magnetic resonance diffusion tensor imaging(DTI),have gradually been applied to the diagnosis of remote site damage.

Objective To investigate the influence of psychological intervention on the anxiety of patients with obstructive jaundice and supply theoretical reference for psychological evaluation and nutsing.Methods 129 patients with obstructive jaundice was divided into the intervention group(64 cases)and the control group(65 cases).The control group adopted routine treatraent method such as anti-infection,liver protection treatment,enhancement of coagulation mechanism and supporting treatment.The intervention group received psychological nursing,relaxation training and distraction of attention based upon routine nursing.The degree of anxiety and pruritus was obseved and recorded in the two groups.Results The degree of anxiety in the intervention group was evidently lower than that of the control group(P＜0.01).The degree of pruritus in the intervention group was 18.75%,which was statistically different from that of the control group(35.38%),P＜0.05.Condusion Adoption of psychological intervention could effectively reduce the degree of anxiety and pruritus of patients with obstructive jaundice.

Objective] From the Chinese point of view of haze-induced lung injury pathogenesis, diagnosis and treatment, it provides theoretical support. [Method] To exogenous disease TCM theory, the nature of the haze of evils, a sense of evil ways and pathogenic characteristics, etc. based on the analysis of the haze of the etiology and pathogenesis of lung injury, the basic pathogenesis are analyzed and discussed. [Results] For haze pathogenic proposed basic principles should be taken to the prevention, prevention is greater than cure, the treatment should be Quxie, and to righten. Work using the method of elimination of pathogens through purgation and churesis.[Conclusion]For haze urgent treatment, prevention and treatment of traditional Chinese medicine for haze-induced lung injury has very important theoretical and practical value.

ObjectiveTo investigate the clinical efficacy of Yaotongxue(Ex-UE7)finger manipulation plus ultrashort waves in treating acute lumbar sprain.MethodForty-two patients with acute lumbar sprain were enrolled and randomized into groups. The control group received lumbar ultrashort waves treatment and the observation group, Yaotongxue finger manipulation in addition. Pain relief and activities of daily living were assessed using the Visual Analogue Scale (VAS) and the Barthel Index before and after one and five sessions of treatment.ResultThere was no statistically significant pre-treatment difference in the VAS score between the two groups (P>0.05). After one and five sessions of treatment, the VAS score was significantly lower in the observation group than in the control group and there was a statistically significant difference (P<0.05); the Barthel Index score was significantly higher in the observation group than in the control group and there was a statistically significant difference (P<0.05). at three months of follow-up, the VAS score and the Barthel Index score were basically normal; there were no statistically significant differences between the two groups (P>0.05). No adverse events occurred during the study.ConclusionYaotongxue finger manipulation plus ultrashort waves is safe and highly effective in treating acute lumbar sprain.

Objective To evaluate the clinical outcome of patients who suffered from acute myocardial infarction with angiographically normal coronary arteries (MINC) Methods Forty patients (mean age: 52 6?12 2 years; range: 30-75 years) admitted with acute myocardial infarction had normal coronary arteries after AMI, defined by normal or insignificant 50% diameter stenosis) The risk factors, clinical data, ventricular functions and the long term outcomes were compared between Group A and Group B Results Hypertension and previous angina were more frequent in Group B Patients in Group A had a lower number of risk factors associated with them than patients in Group B LVEF during hospital was higher in Group A than in Group B The mean follow up period was 67?40 months in Group A and 68?34 months in Group B Incidencs of rehospitalization, repeat intervention therapies, heart failures, and total heart events were fewer in Group A than in Group B Conclusion These data suggest a favorable long term prognosis and fewer risk factors in patients who suffered from acute myocardial infarction with normal or nearly normal coronary arteriogram

Objective Previously, there was an argument on the surgery way of unilateral papillary thyroid microcarcinoma(PTMC).The purpose of this study was to investigate the predictive factors of occult thyroid carcinoma in unilateral thyroid papillary microcarcinoma, and to compare the surgical methods of unilateral thyroid papillary microcarcinoma.Methods We retrospective analyzed 327 cases of unilateral PTMC patients confirmed by preoperative color Doppler ultrasound examination from January 2014 to January 2016 in Department of Breast Surgery, First Affiliated Hospital of Harbin Medical University.According to the presence of occult cancer, patients were divided into unilateral glandular foci (n=278) and bilateral glandular foci(n=49);according to whether carry out the prevention of central lymph node dissection, patients were divided into lymph node dissection(n=117) and without lymph node dissection(n=210) to further analyze the predictive factors of contralateral gland occult cancer and postoperative complications of preventive central lymph node dissection.Results 55 cases had central lymph node metastasis in 117 cases with lymph node dissection, the metastasis rate was 47.0%.The multiple foci in the ipsilateral gland is a risk factor for the presence of occult foci in the contralateral gland (OR=4.924, 95%CI: 1.605~15.107).There was no significant difference in postoperative complications between lymph node dissection and without lymph node dissection(P>0.05).Conclusion multiple foci in the unilateral thyroid papillary thyroid microcarcinoma can help us to predict the contralateral gland occult cancer, and the central lymph node metastasis rate is higher, therefore, for the unilateral glands of multiple tumors in the papillary thyroid microcarcinoma, we recommended the total thyroidectomy+prophylactic central lymph node dissection surgery way.

Objective To summarize our experiences in the long-term continuous maintenance of the peripherally inserted central catheter (PICC) in a patient with intestinal fistula.Method The systematic holistic nursing care has been provided according to the nursing practices to a patient with intestinal fistula since 2006,during which eitht PICCs were used successively.Results The eight PICCs were placed for 3-14 months.The 1 st,3rd,5th,and 7th cathers were retained for 12,13,11,and 14 months.The second catheter was retained for 6 months,and then removed due to increased body temperature (38.7℃).The 4th cather was retained for 3 months,then catheter-related bloodstream infection was suspected due to high body temperature (39℃) ; culture for fungi at the tip showed positive results.The 6th catheter was retained for 7 months,inserting from jugular vein to the superior vena cava; however,it was removed due to puncture point local purulent changes at the puncture site.The 8th catheter was applied for 8 months,but was rmoved due to tube occlusion,which responded poorly to urokinase.Conclusion The application of PICC in patients with fistula can relieve the pain and improve the quality of life.

Objective To explore the value of contrast-enhanced ultrasonography (CEUS) in diagnosis of esophagogastric varices (EGV) compared with color Doppler flow imaging (CDFI). Methods CDFI and CEUS were performed on 23 variceal patients based on the B mode ultrasound examination. The blood flow of esophageal varices (EV) or gastric varices (GV) displayed with CDFI and CEUS were compared. Results The flow signal demonstration rate of EV with CDFI and CEUS was 42.11% (8/19) and 73.68% (14/19), while that of GV with CDFI and CEUS was 76.47% (13/17) and 94.12% (16/17), respectively. CEUS displayed the flow signal of EV better than CDFI did (χ~2=4.17, P＜0.05), but the flow signal demonstration rate of GV was not significantly different (χ~2=1.33, P＞0.05). Conclusion CEUS can be used more extensively in clinic practice for diagnosis of EGV.

Thyroidectomy is an effective treatment for thyroid diseases,especially thyroid malignancies.The postoperative phonological disturbances in patients with recurrent laryngeal nerve injury caused by vocal cord paralysis have been recognized.However,in clinical work,many patients in the absence of recurrent laryngeal nerve injury in the case of postoperative speech changes.With the development of modern electronic acoustics detection technology,the quality of postoperative recurrent laryngeal nerve injury patients with quantitative research,to explore and found that recurrent laryngeal nerve injury after thyroid surgery is not the voice of the factors.