Objective To investigate diagnostic value of ectopic pregnancy by transvaginal sonography (TVCDS) combining with detection of blood β-human chorionie gonadotropin(β-HCG) and progesterone(P) level. Methods 96 ectopic pregnancy patients were exahined by TVCDS,and detected β-HCG and P in the same day or next day,every two days or several days reexamination of β-HCG and P were given to the hospitalization patients. Re-suits 96 ectopic pregnancy patients including massive type 76(79.1%) ,diameter (47.6±18.4) mm;embryo sac type 20(20.9%) ,showed embryo sac structure in ectopic pregnancy area,saw pulsation in saccus vitellinus and fetus vascular color,diameter (12.86±6.85)mm. 94(97.9%) displayed Doppler signals in ectopic pregnancy area, and 89(92.7%)cases were detected low resistivity nutrient artery flow spectrum by PW, the PI average value was (0.46±0.03).96 ectopic pregnancy patients' blood β-HCG and P level were significant low(P<0.01). Conclusion TVCDS combining with detection of β-HCG and P can raise early diagnosis level of ectopie pregnancy.