In the treatment of 80 cases of the patients with deficient and cold pattern in the spleen and stomach by external application of herbal paste on the acupoints, the results showed remarkable effect in 52 cases, improvement in 24 cases, failure in 4 cases and the total effective rate in 95.0%. Remarkable therapeutic effects were achieved in three different diseases of same pattern. External application of herbal paste on the acupoints is definitely effective for stomachache in deficient and cold pattern of the spleen and stomach.

OBJECTIVE To know the gene type of Staphylococcus aureus which caused the respiratory tract infection of patients after transplantation in our hospital,and to explore the epidemiology,preventing measure and nursing strategy of the lung infection by S.aureus among them.METHODS Using random amplified polymorphic DNA(RAPD) to analyze the gene type fingerprint of the 20 S.aureus strains originated from the respiratory tract of patients after transplantation,and using cefoxitin paper strip method to detect the mecA gene of the S.aureus.RESULTS Twenty S.aureus strains were divided into 8 gene types,and among them,the detectable rate of gene types Ⅰ,Ⅱ,and Ⅲ was 40.0%,15.0%,and 15.0%,respectively.The positive rate of mecA gene reached to 80.0%.CONCLUSIONS The S.aureus originated from respiratory tracts of the patients after transplantation is mainly of gene type Ⅰ.And the positive rate of mecA gene is very high.So the management of hands washing,sterilization and isolation of medical and nursing staffs should be strengthened,and effective method should be took to prevent the propagation of mecA positive S.aureus among the patients after transplantation.

0.05) ;incidence of aleucocytosis was 43.3% vs.27.5%(P

Objective To investigate the clinical application of statins for secondary prevention and its efficacy in patients with ischemic stroke.Methods A total of 300 patients with ischemic stroke were divided into statins group (n=91) and control group (n=209) according to whether statins was received or not.Serum lipid parameters,liver and kidney function,carotid artery intimamedia thickness (IMT) and plaque area were compared between the two groups.Results In 209 patients not taking statins,68 cases (32.5％) were not advised by clinic doctor; 55 cases (27.8％)were reluctant to take statins in fear of adverse reactions; 77 cases (36.8％) discontinued stains when serum lipids were normal; 3 cases (1.4％) developed skin allergic reaction and the skin rash that disappeared after stains discontinuance; 3 cases (1.4％) had elevated liver enzyme levels and the enzyme levels were declined to normal after stains discontinuance.The levels of total cholesterol (TC),triglyceride (TG) and low density lipoprotein cholesterol (LDL-C) were significantly lower in statin group than in control group [(4.93±0.45) mmol/L vs.(5.87±0.38) mmol/L,(1.68±0.61)mmol/L vs.(2.01±0.58) mmol/L,(2.49±0.57) mmol/L vs.(3.76±0.44) mmol/L,t=18.60,4.46,20.94,P=0.000,0.007,0.000,respectively].High density lipoprotein (HDL-C) level was significantly higher in stain group than in control group [(1.36±0.31) mmol/L vs.(1.03±0.25)mmol/L,t=9.75,P=0.001].There were no significant differences in liver and kidney function parameters such as alanine aminotransferase,aspartate aminotransferase,alkaline phosphatase,serum total bilirubin,direct bilirubin,indirect bilirubin,glutamyl transferase,blood urea nitrogen and serum creatinine between the two groups (all P＞0.05).The carotid IMT and carotid atherosclerotic plaque area were significantly lower in stain group than in control group [(1.62 ± 0.23) mm vs.(1.74±0.31) mm,(0.57±0.12) cm2 vs.(0.65±0.18) cm2,t=3.32,3.88,P=0.008,0.002,respectively].Conclusions Most patients with ischemic stroke are still not taking statin for secondary prevention.Statins can improve the blood lipid levels and carotid arteriosclerosis status,and no obvious adverse reactions are observed.

[Objective]To investigate differential gene expression in dendritic cell(DC) in response to human alloantigen and cytomegalovirus protein,and search target genes which can prevent graft rejection and eytomegalovirus (CMV) infection.[Methods]Genome-wide microarray analysis was performed to test gene expression in DC in response to human alloantigen and cytomegalovirus protein 3A(CMV3A).[Results]The Results showed that the difference of gene expression of DC induced by CMV3A and alloantigen was significant.The genes with differential expression included antigen processing and presentation,toll-like receptor signaling pathway and cell movement/migration, cytokine-cytokine receptor interaction and metabolism of xenobiotics by cytochrome p450,and several heat shock protein(HSP) family members including HSPA5,HSPA8,HSPA9B and HSP90AB1,in DC induced hy alloantigen were higher expression than that by CMV3A.[Conclusion]This study found several genes including heat shock protein family in DC induced by alloantigen were higher expression than that by CMV3A,these genes might play a valuable role in preventing graft rejection and CMV infection.

This paper is to investigate the effect of ischemic preconditioning on cardiac function in the hyperlipidemic rats.The alterations of LVSP,(d p /d t ) max , GSH Px, SOD and MDA were measured in ischemia/reperfusion group, ischemic preconditoning group and control group with in vitro myocardial ischemic precondioning model. The level of LVSP, (d p /d t ) max , GSH Px and SOD was significantly higher in the preconditoning group than that in the ischemia/reperfusion group, and these values were close to those in the control group. The area of contraction component in P cycle was higher and the amount of MDA was lower, whereas the level of LVSP, (d p /d t ) max declined significantly in ischemia/reperfusion group.It is suggested that ischemic preconditoning in hyperlipidemic rats can reduce cardiac functional impairment due to ischemia/reperfusion injury, and may have myocardial protective effects.

Objective To evaluate the association between serum cystatin C and carotid atherosclerosis in patients with type 2 diabetes mellitus.Methods A total of 318 patients with type 2 diabetes mellitus were enrolled in this study.According to the results of carotid color Doppler ultrasound in the patients were randomly divided into two groups:CIMT abnormal group (CIMT was equal to or larger than 0.9 mm,with or without associated with carotid artery plaque formation)CIMT and normal group (CIMT <0.9 mm,and it was not associated with carotid artery atherosclerotic plaque formation.Differences in serum cystatin C,serum lipid and lipoprotein,fasting plasma glucose and other biochemical markers between the two groups were analyzed by using Student's t test.The correlated factors of the carotid thickness and plaque appearance were analyzed by Logistic regression analysis.Results The serum cystatin C levels of the CIMT abnormal group were higher than the normal CIMT group[(1.24 ±0.22)mg/L vs.(1.05 ±0.41)mg/L, t =4.949,P <0.001).Multivariate logistic regression analysis showed adjusted for gender,age and other factors,ser-um cystatin C was independently associated factor of CIMT (OR =2.563,95% CI:1.026 ~6.403,P =0.044). Conclusion Our data suggest that serum cystatin C is significantly associated with carotid atherosclerosis.

Objective To study the epidemiological and clinical features of human parainfluenza virus (HPIV) infection in children in Suzhou,and to provide the evidence-based foundation for early warning,diagnosis and treatment of respiratory infection in children.Methods The sputum specimens and medical history were obtained from children with acute respiratory tract infection hospitalized at the Childen's Hospital Affiliated to Soochow University from January 2006 to December 2015.Seven kinds of common respiratory viruses including respiratory syncytial virus,influenza virus A,influenza virus B,HPIV Ⅰ,HPIV Ⅱ,HPⅣV Ⅲ and adenovirus were detected by using the direct im-munofluorescence.Mycoplasma pneumoniae(MP),chlamydia pneumoniae,human bocavirns (hBoV) were detected by using fluorescence quantitative PCR.Rhinovirus and human metapneumovirus were detected by using reverse transcription-PCR.Sputum was cultured for bacteria.Results In 21 769 cases,the detection rate of HPIV positive was 3.21％ (829 cases),among which,HPIV Ⅰ,HPIV Ⅱ,HPIV Ⅲ were respectively detected in 113 cases (0.52％),16 cases (0.07 ％) and 700 cases (3.21％),respectively.There were 378 cases of simple infection and 428 cases of mixed infection,and the mixed infection was very common in Streptococcus pneumoniae,Haemophilus influenza,MP and hBoV.There was a difference in HPIV infection among genders,and the detection rate of the boys was higher than that of girls[4.14％ (563/13 591 cases) vs.3.25％ (266/8 178 cases),x2 =11.036,P =0.001].In the 28 d-1 year old and ＞ 1-3 year old group,the detection rate of HPIV was higher[4.71％ (494/10 476 cases) and 4.21％ (244/5 793 cases),respectively].In spring and summer,there was a higher detection rate of HPIV infection.The clinical manifestations with simple infection of HPIV Ⅰ and HPIV Ⅲ were cough,fever and wheezing.The rate of fever and shortness of breath in those of HPIV Ⅰ was 71.74％ (33/46 cases),10.87％ (5/46 cases),and that in HPIV Ⅲ was 40.12％ (134/334 cases),2.10％ (7/334 cases),HPIV Ⅰ infection was more likely to cause fever and shortness of breath than those of HPIV Ⅲ,there were significant differences (x2 =16.410,P ＜ 0.001;x2 =10.177,P =0.001).Pneumonia had the highest detection rate of viral infection.Conclusions HPIV Ⅲ is the leading pathogen among the types of HPIV in the hospitalized children in Suzhou area.Among the subtypes of HPIV,the peak of HPIV infection occurs in spring and summer.The children less than 3 years old are the most susceptible to parainfluenza virus,and the HPIV detection rate is gradually declines with age.

A method of ultra performance liquid chromatography-quadrupole-time of flight-mass spectrometry ( UPLC-Q-TOF MS) was developed to determine 35 kinds of illegally added chemical fungicides in pesticide formulations. The samples were pretreated based on the ultrasonic extraction by the solvent of methanol, and then separated on a Zorbax C18(100 mm×1. 8 mm, 2. 1 μm) column by a gradient elution with 0. 1% formic acid aqueous solution and acetonitrile as the mobile phase. The mass spectrometer was operated under positive mode. Under the optimal conditions, the recoveries at three spiked levels (0. 2, 0. 4, and 2. 0 mg/kg) were in the range of 81. 0%-101. 3% and the RSDs were 1. 0%-4. 4%. Based on the developed method, 100 samples were analyzed, and among which 6 samples were screened out chemical fungicides. The proposed method was high-efficient, accurate and reliable for the qualitatively screening of illegaly added chemical fungicides.

OBJECTIVE:To observe the analgesia effects of transdermal fentanyl in the treatment of pharynx oralis pain caused by radiotherapy.METHODS:30patients with pate squamous carcinoma and serious membrana mucosa ulcerate and pain after a whole range radiotherapy,who were treated with3to5patches2.5mg(25?g/h)of transdermal fentanyl every9～15days if their VAS scores were found to be more than6scores.The analgesia effect,life quality and the adverse reaction were evaluated respectively with VAS,Digit-scale and east American carcinoma cooperation group toxicity criteria.RESULTS:The average VAS scores has been lowered from7.86?1.18before the treatment to2.24?1.31on10th day of the treatment(P