Objective To study the relationship of plasma BNP monitoring and cardiac function classification in elderly patients with ST-segment elevation acute myocardial infarction which intravenous thrombolysis of the early period.Methods 92 patients with acute myocardial infarction who got recanalization therapies by intravenous thrombolysis within 6 hours after myocardial infarction were selected.All the patients were divided into four groups by Killip classification criteria,the BNP level was compared.Comparison of plasma BNP levels in patients with different LVEF,and statistical analysis were conducted.Results With the increase of cardiac function classification,AMI in patients with BNP levels gradually increased (F =56.144,P ＜ 0.05),LVEF reduction in AMI serum BNP levels gradually increased,the difference was statistically significant (F =79.234,P ＜ 0.05).Conclusion The plasma BNP levels of patients with acute myocardial infarction was higher,the heart function was lower.BNP can reflect the heart function,and can be served as an effective marker of clinical diagnosis observation of therapeutic effect,risk stratification and judgement of prognosis in patients with acute myocardial infarction.

[Objective] To observe clinical cure effect of revised Yijing Decoction treating deficient menopause.[Method] Take revised Yijing Decoction for treatment of 34 cases and comparison.[Result] 18 cases were cured(53%),12 better(35.3%),4 had no effect(11.7%),the total effective rate was 88.3%.[Conclusion] The revised Yijing Decoction can effectively treat deficient menopause.

Objective To establish a model of DRG-like grouping of cesarean section patients in the Beijing area so as to observe its feasibility of controlling rising medical costs.Methods DRG-like grouping of cesarean section patients was conducted by case-mix/DRG.The patients were divided into 3 groups according to complications: the serious complication group,the moderate complication group,and the minor or no complication group.Results The rate of cesarean sections remained at 50% or so in Beijing.A statistical analysis showed a significant difference between the 3 groups in costs and length of hospital stay.70% of the cases in the serious complication group belonged to the high cost group whereas 70 % of the cases in the minor or no complication group belonged to the low cost group.Conclusion The main ways of controlling rising costs for cesarean sections include reducing the rate of cesarean sections and(adopting) DRG-like PPS payments in view of the nature of medical insurance and government payments for cesarean sections.

OBJECTIVE:To establish a HPLC method for the determination of paracetamol,caffeine and aspirin in compound paracetamol tablets.METHODS:The chromatographic column was ODS,the mobile phase was methanol-4.2% glacial acetic acid solution(3∶7)with a flow rate at1.0ml/min;the detecting wavelength was 275nm,the sample size was 20?l and the column temperature was 25℃.RESULTS:The linear ranges of paracetamol,caffeine and aspirin were 16.08～428.80?g/ml(r=0.9981),3.42～91.20?g/ml(r=0.9988) and 23.87～769.92?g/ml(r=0.9999) respectively;The average recovery were 99.2%(RSD=0.23%),100.4%(RSD=0.63%)and 99.3%(RSD=0.11%)respectively.CONCLUSION:This method was simple,fast and accurate,which can be used for the content determination of compound paracetamol tablets.

The recent research of opioid receptors has achieved great progress, especially in the field of the protection of cardiac muscles in ischemic preconditioning, which makes people pay more attention to the relation ship between opioid receptors and cardiovascular system. This article summarizes the studies of opioid receptors, the measures they take to modulate cardiovascular system and the way they participate in ischemic proconditioning both at home and abroad.

AIM:To explore the effect of the combination of ACEI and ARB on left ventricle in pressure-overloaded hypertrophy rats, and also the relationship between left ventricular hypertrophy (LVH) and cytokines, matrix metalloproteinases, apoptosis, and apoptosis genes. METHODS: IL-6, IL-10, MMP-2 and MMP-9 were evaluated by ELISA method. TNF-alpha was measured with radioimmunoassays. Flow cytometer was used to measure the apoptosis ratio and the expression of Fas, Bcl-2 and Bax. RESULTS: 6 weeks after abdominal aortic coarctation operation, the weight of the left ventricle ( 0.983 ? 0.316 g ) and heart weight/body weight ( 0.382 ? 0.154 ) were significantly increased in control group compared with the sham operated group. And also IL-6 ( 208.71 ? 84.51 pg?ml -1 ), TNF-? ( 1.983 ? 0.842 ng?ml -1 ), MMP-2 ( 74.63 ? 37.62 ), MMP-9 ( 57.74 ? 25.61 ), apoptosis ratio ( 8.47 ? 4.18 ) and apoptosis genes (fas, bax, bcl-2, bcl-2/bax) expression were increased, but IL-10 ( 86.23 ? 40.13 pg?ml -1 ) was decreased compared with sham operated group. The 3 groups with drugs (CP, LT, CP+LT) decreased the increasing weight of LV and ventricular weight/body weight and inhibited the changes of influencing factors (IL-6, IL-10, TNF-?, MMP-2, MMP-9, apoptosis ratio and apoptosis genes expression), especially in the group of combination of 2 drugs. CONCLUSION: LVH might be related with many influencing factors. ACEI and ARB may have the positive importance in the treatment and prevention of LVH.

Objective To investigate the inhibitory effect of siRNA targeting casein kinase 2 (CK2α)gene on the growth of HCT1 1 6 cells and to clarify its mechanism.Methods CK2α-siRNA sequence was designed according to mRNA sequence of CK2α. The in vitro cultured HCT1 1 6 cells were divided into normal control group (without transfection),negative control group (transfected with siRNA)and CK2α-siRNA group (transfected with CK2α-siRNA ),and the HCT116 cells were transfected with Lipofectamine 2000.The expression levels of CK2α,cyclin H,P53,and P21 proteins in the HCT116 cells were detected by Western blottting method,and the proliferation activities of the HCT116 cells were detected by MTT method,and the cell cycle was measured by flow cytometry. Results Compared with negative control group,the expression levels of CK2α,and cyclin H proteins in CK2α-siRNA group were decreased(P<0.01);the expression level of P53 protein had no change dramatically(P>0.05), and the expression level of P21 protein was increased significantly(P<0.01).Compared with negative control group,the survival rate in CK2α-siRNA group was decreased markedly 48 and 72 h after transfection detected by MTT method(P<0.01).Flow cytometry analysis showed the percent of the cells at G1 phase in CK2α-siRNA group was significantly higher than that in negative control group and the percent of the cells at S phase in CK2α-siRNA group was lower than that in negative control group(P<0.01),and the cell cycle was arrested at G1 phase. Conclusion siRNA targeting CK2αcan inhibit the proliferation of HCT116 cells and induce the arrest of G1 phase, which may be associated with inhibiting the expression of cyclin H and recovering the P53 activity after silencing CK2α.

Purpose:To evaluate the efficacy and toxic and side effects of brain metastases of lung cancer treated with chemotherapy and conventional radiotherapy. Methods:45 cases of brain metastases of lung cancer were treated with sequential chemotherapy of VM26DDP regimens and conventional radiotherapy. The complete remission and partial remission rate ,1-,2-,3-year survival rate and toxic and side effects were observed.Results:The complete remission and partial remission rate of salitary as well as multiple brain metastases of non-small-cell lung cancer and small-cell lung cancer were (92.86%) and 80.94%,75% and 83.33%, respectively . The 1-,2-,3-year survival rate of salitary and multiple brain metastases of non-small-cell lung cancer was 13.33% and 14.29%、9.5% and 4.76%、9.5% and 0. The 1-,2-,3-year survival rate of salitary and multiple brain metastases of small-cell lung cancer were 50% and 50%、25% and 33.33%、25% and 16.67%, respectively. The Ⅰ,Ⅱ,Ⅲ and Ⅳ degree myelosuppression was 33.33%,28.89%,17.78%,8.89%, respectively.Conclusions:The efficacy of brain metastases of lung cancer treated with sequential chemotherapy by VM26+DDP regimens and conventional radiotherapy is better and with less toxic and side effects.

OBJECTlVE To study the preventive effect of vitamin E(Vit E)on chronic Iiver injury in-duced by N,N-dimethyIformamide( DmF). METHODS HeaIthy maIe Kunming mice were randomIy divided into 4 groups,10 mice in each group. The mice in DmF modeI group and DmF+Vit E group were ig given DmF 0.5 g·kg-1 ,whiIe Vit E controI group and DmF+Vit E group were ig given Vit E 5 mg·kg-1 after 2 h once a day for 30 d. The activity of serum gIutamic-oxaIoacetic transaminase(GOT),gIutamic-pyruvic transaminase(GPT)and xanthine oxidase(XOD)and the content of maIondiaIdehyde(mDA) in Iiver tissue were measured with corresponding kits. The pathoIogicaI changes of Iiver tissue were ob-served with HE staining. RESULTS Compared with the normaI controI group,the activity of serum GOT,GPT and XOD of the DmF modeI group was significantIy eIevated(P﹤0.05,P﹤0.01),and the Iiver ceII nucIeus pycnosis,ceIIuIar edema,degeneration,necrosis occurred. Compared with DmF modeI group,the activity of serum GOT,GPT and XOD of DmF+Vit E group were aII significantIy reduced(P﹤0.05,P﹤0.01),the content of mDA in Iiver tissue was significantIy diminished(P﹤0.01),but histopatho-IogicaI changes of Iiver tissue were significantIy improved. CONCLUSlON Vit E has preventive effect on chronic Iiver damage induced by DmF through antioxidation.

Objective: To synthesize 10-position schiff base derivatives of emodin and study the anti-tumor activity. Methods:The 10-position schiff base derivatives of emodin were synthesized through emodin reacting with ammonia derivatives in absolute alcohol with acetic acid as the catalytic agent. Their structures were characterized by 1 H-NMR and ESI-MS, and the anti-tumor activity on k562 cells was determined by MTT method. Results:Three 10-position schiff base derivatives were synthesized and the structures were confirmed, and the IC50 on k562 cells was 4. 5, 3. 8 and 2. 0 μmol·L-1 , respectively. Conclusion:The 10-position schiff base de-rivatives of emodin can improve the anti-tumor activity and druggability of the parent compound, which are worthy of further study.