The ras-mitogen activeted protein kinase pathway is responsible for regulation of many cel-lular processes, including differentiation, cytoskeletal organization, and protein trafficking. Much attention has been paid to them and their role in cancer therapy recently, and its inhibitor pathways are applied to clinical tes-ting, such as, Raf kinse inhibitors, MEK inhibitor, Src kinase inhibitor.

OBJECTlVE To study the preventive effect of vitamin E(Vit E)on chronic Iiver injury in-duced by N,N-dimethyIformamide( DmF). METHODS HeaIthy maIe Kunming mice were randomIy divided into 4 groups,10 mice in each group. The mice in DmF modeI group and DmF+Vit E group were ig given DmF 0.5 g·kg-1 ,whiIe Vit E controI group and DmF+Vit E group were ig given Vit E 5 mg·kg-1 after 2 h once a day for 30 d. The activity of serum gIutamic-oxaIoacetic transaminase(GOT),gIutamic-pyruvic transaminase(GPT)and xanthine oxidase(XOD)and the content of maIondiaIdehyde(mDA) in Iiver tissue were measured with corresponding kits. The pathoIogicaI changes of Iiver tissue were ob-served with HE staining. RESULTS Compared with the normaI controI group,the activity of serum GOT,GPT and XOD of the DmF modeI group was significantIy eIevated(P﹤0.05,P﹤0.01),and the Iiver ceII nucIeus pycnosis,ceIIuIar edema,degeneration,necrosis occurred. Compared with DmF modeI group,the activity of serum GOT,GPT and XOD of DmF+Vit E group were aII significantIy reduced(P﹤0.05,P﹤0.01),the content of mDA in Iiver tissue was significantIy diminished(P﹤0.01),but histopatho-IogicaI changes of Iiver tissue were significantIy improved. CONCLUSlON Vit E has preventive effect on chronic Iiver damage induced by DmF through antioxidation.

Objective To evaluate the pharmacokinetic parameters and bioavailability of Cyclobuxine D in transdermal patch in Newzealand rabbits by determining concentration-time curve and by comparing with the pharmacokinetics of Cyclobuxine Dinjection and suspension.Methods Precolumn derivatization RP-HPLC was used to detect the concentration of Clovirobuxine D in rabbits plasma at different time,and software 3p87 was used to analyze the pharmacokinetics parameter.Results In contrast to oral delivery,relatively steadily sustained blood concentration with minimal fluctuation and prolonged peak time were presented in the rabbits over a long period after transdermal administration.The absolute bioavailability of Cyclobuxine D was 30.472 %.Conclusion Cyclobuxine D Patch exhibits good controlled-release properties and maintains appropriate blood concentration for a prolonged time.

Objective: To study the quality of life of chronic elderly patients Method: 150 chronic patients aged 60 or above were assessed with SCL-90 (symptom checklist-90), SDSS (social deficit screening scale), ADL (activity of daily living) and QOL (quality of life), their demographic data were also collected Results: Men's social function was poorer than women's, but their quality of life had no significant difference to women's The quality of life in our sample differed with different age, educational degree, family conditions, occupations and incomes Conclusion: Life quality of chronic old patients is related to their demographic characters

Objective:To evaluate the in vitro and in vivo immunostimulatory activity and the safety of ethanol extract of wild Artemisia rupestris L. (EEWAR). Methods:Bone marrow dendritic cells (BMDCs) from C57BL/6 mice were treated with different concentrations of EEWAR in vitro and the expression of CD40 and CD80 on BMDCs was detected by flow cytometry. ICR mice were subcutaneously immunized with different concentrations of EEWAR in combination with ovalbumin (OVA) or OVA alone. Aluminum adjuvant was used as the positive control. OVA-specific IgG antibodies in mouse serum samples were measured by ELISA following immunization. T cell proliferation in spleen tissues was detected by MTT method. Acute toxicity test was conducted in ICR mice to analyze the safety of EEWAR. Results:In vitro experiment showed that EEWAR at the concentrations of 10-20 μg/ml increased the expression of CD40 and CD80 on BMDCs ( P<0.05), and had no significant effect on the morphology of BMDCs; EEWAR at the concentrations of 100-200 μg/ml significantly promoted the expression of CD40 and CD80 on BMDCs ( P<0.01), but had a certain influence on the morphology of BMDCs. In vivo experiment showed that EEWAR enhanced the production of IgG, IgG 1 and IgG 2a antibodies against OVA and the proliferation of splenocytes ( P<0.05). In the acute toxicity test, EEWAR at the concentrations of 50-5 000 μg/ml had no side effects on mouse body weight and was relatively safe. Conclusions:EEWAR could promote the maturation of DCs and enhance the humoral and cellular immune responses when used as an adjuvant to OVA. It was safe in a certain dose range. This study provided reference for further research on EEWAR as a new-generation adjuvant.

Objective To compare the clinical efficacy of through moxibustion combined with electric acupuncture versus electric acupuncture alone for treatment of patients with cervical spondylosis radiculopathy (CSR), so as to provide a basis for optimizing clinical treatment.Methods A prospective study was conducted. Sixty patients with CSR treated in the clinic of Acupuncture Department as outpatients or admitted into the department of the First Affiliated Hospital of Xinjiang Medical University as inpatients were enrolled, and according to the random number table, they were divided into through moxibustion combined with electric acupuncture treatment group (observation group) and conventional electric acupuncture treatment group (control group), 30 cases in each group. The main acupoints of the observation group were as follows: Fengchi, Dazhui, Tianzhu, Houxi, Cervical Jiaji points, Shenshu, Mingmen, in cases with significant neck and shoulder pain, supporting points were added such as Jianjing, Tianzong, in cases with severe arm and finger numbness and pain, Quchi, Hegu, Waiguan, and in cases with marked dizziness and headache, Baihui, Temple acupoints were added. The needle 0.30 mm × 40 mm in size was chosen to be applied, at bilateral neck Jiaji points, direct subcutaneous needling (piercing) was made, at Dazhui, the piercing of needle for 1 to 1.5 inches was performed (body inch), at Mingmen, reinforcing method was used for piercing, at Shenshu, 0.30 mm × 50 mm needle was chosen for piercing with reinforcing method, and at the rest acupoints, conventional neutral supplementation and drainage method of acupuncture was applied. After obtaining qi from needling, electric needle device was added, the current strength used for individual patient depended on his or her degree of tolerance, the needle retention being 20 minutes, and during the above process, at Dazhui and a set of neck Jiaji points, moxibustion with moxa was applied. The acupoints used in the control group were the same as those in the observation group except moxibustion, other procedures were similar to those in the observation group. In both groups, the therapy was taken once a day for consecutive 5 days in a week, constituting one therapeutic course, and after two courses, the changes of clinical symptom scores and clinical efficacies were observed.Results After treatment, the clinical symptom scores in two groups were significantly higher than those before treatment (control group: 15.68±2.01 vs. 7.10±1.87, observation group:18.13±1.69 vs. 7.92±2.14), and the degree of improvement in the observation group was more significant (P < 0.01). After treatment, the total effective rate in observation group was significantly higher than that in the control group [96.67% (29/30) vs. 83.33% (25/30),P < 0.05].Conclusion The clinical therapeutic effect of through moxibustion combined with electric acupuncture for treatment of patients with CSR is better than that of electric acupuncture alone, so there is certain feasibility.

Objective To study the influences of psycho-social factors in the treatment of the aged patients with hypertension. Methods Aged in-patients with hypertension were divided into two groups according to their improvement: 148 reached the standard and 62 did not reach the standard. Their psycho-social factors were assessed by self-rating depression scale(SDS), self-rating anxiety scale(SAS), life events scale (LES) and social support scale. Results (1)The percentages of depression (7.4% vs 32.2%, P

Objective To investigate the expression and prognostic significance of leucine-rich and immunoglobulin-like domains 1 (LRIG1) and epidermal growth factor receptor (EGFR) in cervical adenocarcinoma.Methods The expression levels of LRIG1 and EGFR were assayed by immunohistochemistry in 47 cervical adenocarcinoma,24 cervical glandular intraepithelial neoplasia (CGIN) and 60 normal cervical tissues.Results The expression rates of LRIG1 in cervical adenocarcinoma,CGIN and normal cervical tissues were 21.28 ％ (10/47),29.17 ％ (7/24) and 83.33 ％ (50/60),respectively,the expression of EGFR in cervical adenocarcinoma,CGIN and normal cervical tissues were 82.98 ％ (39/47),45.83 ％ (11/24),5.00 ％ (3/60),respectively.The expression of LRIG1 was not correlated to age (P ＞ 0.05).However,it was correlated to histological grade,lymphatic nodes metastasis and clinical stages (all P ＜ 0.05).The survival rate of positive LRIG1 patients was higher than that of negative LRIG1 patients (P ＜ 0.05).Conclusions LRIG1 may play an important role in predicting clinical outcomes of cervical adenocarcinoma,and its mechanism may be related to inhibiting EGFR signal transduction.

Objective To explore the neural basis of the visual illusory motion using the event-related potential(ERP).Methods One hundred trials(each duration 2 000 ms)of a visual illusory motion figure,rippling wheat pattern made by Akiyoshi Kitaoka,and a visual static figure made of modified rippling wheat pattern were randomly presented with equal probability.Ten healthy right handed undergraduate students as subjects were asked to judge the stimulus whether motional or not.The EEG was recorded from 128 scalp sites using with electrodes mounted in HydroCel GSN cap(Electrical Geodesics Incorporated,Oregon,USA).Results 1)The C1 component of ERP could be evoked by both visual illusory motion pattern and static pattern in POz.The peak of C1 component was presented about 75 ms after each stimulus.C1 component evoked with visual illusory motion was negative,but it's static pattern to be positive.2)P100 and P200 components could be obviously evoked by both kinds of stimuli,but in O2 the amplitudes of P100 evoked by different stimuli were significant different and in T3 the amplitudes of P200 were significant different too.Conclusion The visual illusory motion is formed at the primary visual cortex.It may be related to the organized mode of perception.

Objective To explore the relationship between the types of female breast density and age and breast cancer .Methods By accepting the digital mammography X -ray examination for 5 006 women cases and according to the ACR BI -RADS standard in the fourth edition ,the breast density assessment was quantified . We analysed the relationship between the breast density and age and breast cancer .Results In 5 006 cases,the average female age was between 44.22 ±8.09 years old,median age was 43 years old.The components of the breast density were fat type , small amount type , large amount type and compact type each count were 256 (5.11%),726(14.51%),3 719(74.29%),305(6.09%)respectively.By dividing into different age -group to analyze the breast density,there was significant statistical differences of the breast density among age -groups(P<0.001).Among them the breast cancer were 184 cases.Age was between 51.26 ±10.15 years old.Breast cancer in each breast density were fat type 10.16%(26/256),small amount type 9.09%(66/726),large a-mount type 2.45%(91/3719)and compact type 0.33%(1/305).There were statistical differences among age -groups and breast densities and breast (P<0.001).Conclusion Age plays a very important effects on the fe-male breast density .The lower breast density is a high risk factor to breast cancer occurrence .