Epithelial-mesenchymal transition (EMT) is the basis of biological process of embryonic development,and is also closely related with tumor cell in situ invasion and distant metastasis.Recent studies find that EMT and cancer stem cells (CSCs) have a close relationship.CSCs have a strong ability to selfrenewal,tumorigenicity and cell differentiation potential.Identifing the markers,in-depth study of CSCs resistance,may provide a new path for cancer treatment.

Objective To investigate the effect of different composed combination of EGCG and cisplatin on human colon adenocarcinoma cell line SW620 and the related mechanisms. Methods SW620 cells at exponential phase were treated with cisplatin(5 mmol.L-1)and EGCG(0, 25, 50 and 100 mmol.L-1).Seventy hours after the cultivation, cell proliferation was detected with MTT;the percentage of apoptotic cells was determined by flow cytometry;expression levels of JAK2, p-JAK2, p-STAT3, STAT3, Bax, Bcl-2, cleavage-PARP, and cleavage-caspase3 were detected by Western blotting. Results The SW620 cell proliferation rate was(95±5)%,(67±5)%,(42±5)%(22±4)% and the apoptotic rat was 5%, 15%, 35%, 57%in the 0, 25,50, 100 mmol.L-1 EGCG groups, respectively.Moreover, EGCG up-regulated the expression of Bax, cleavage-PARP and cleavage-caspase3(P<0.05)and down-regulated the expression of p-JAK2, p-STAT3 and Bcl-2(P<0.05)in a dose-dependent manner, but had no influence on the expression of JAK2 and STAT3(P>0.05). Conclusion EGCG can promote cisplatin-induced apoptosis and proliferation inhibition of human colon adenocarcinoma cell lines of SW620 by suppressing JAK2/STAT3 gignaling pathway.

Objective To determine the pharmacokinetics and pharmacodynamics of chloroprocaine with or without epinephrine for epidural blockade. Methods Twenty ASA physical status Ⅰ or Ⅱ patients of both sexes aged 37-55 yrs weighing 45-80 kg undergoing elective lower abdominal surgery were randomly divided into 2 groups (n = 10 each): group C chloroprocaine without epinephrine and group CE chloroprocaine with epinephrine. The epidural catheter was inserted into epidural space at T12-L4 and advanced toward head for about 4 cm. After correct epidural placement was confirmed,3% chloroprocaine 6 mg?kg-1 with or without 1:200 000 epinephrine was injected into epidural space over 2 min. Onset time of analgesia , motor blockade and degree of motor blockade at 20 min after epidural injection (Bromage scale 0 = no motor block, 3 = unable to flex hip, knee and ankle) were recorded. Blood samples were taken from radial artery before and at 3, 6, 9, 11, 13, 15, 17, 20, 30, 45, 60, 90 min after epidural injection for determination of plasma chloroprocaine concentration by HPLC. Results The two groups were comparable with respect to demographic data. There were no significant differences in the pharmokinetics and pharmacodynamics of chloroprocaine between the two groups. The Cmax was (0.491?0.47) mg?L-1 and (0.32?0.22) mg?L-1; The Tmax was (8?3) min and (9?4) min; the AUC was (10?6) ?g?min?ml-1 and (7?4) ?g?min?ml-1;the K was 0.32?0.21 min-1 and 0.36?0.32 min-1 in group C and CE respectively. Conclusion Epinephrine 1:200 000 does not affect the pharmacokinetics and pharmacodynamics of 3 % chloroprocaine for epidural block.

AIM:To observe the effects of liraglutide on the level of microRNA-33 (miR-33) and the expres-sion of AMP-activated protein kinase ( AMPK ) and apoptosis-related proteins in mice with type 2 diabetes mellitus (T2DM), and to explore its possible mechanism .METHODS:High-fat diet and intraperitoneal injection of streptozocin were used to establish the type 2 diabetic model in C57BL/6 mice.The mice were randomly divided into 4 groups ( n=15 ):in control group , the normal mice were subcutaneously injected with equivalent volume of saline ;in model group , the T2DM mice were subcutaneously injected with equivalent volume of saline ; in low-and high-dose liraglutide treatment groups, the T2DM mice were subcutaneously injected with 100 and 200 μg? kg -1? d-1, respectively.After 4 weeks of administration, the levels of FBG, TG, TC, HDL-C, LDL-C, ALT and AST were determined.HE staining was used to ob-serve the pathological changes of the liver tissues .The protein level of cleaved caspase-3 in the liver tissue was detected by the technique of immunofluorescence .The protein levels of p-AMPK/AMPK and apoptosis-related proteins were detected by Western blot .The expression of miR-33 in the liver tissues was detected by real-time PCR.RESULTS: Compared with model group, the contents of FBG, TG, TC, LDL-C, ALT and AST were decreased significantly , while the content of HDL-C was increased significantly in low-dose liraglutide group and high-dose liraglutide group ( P<0.05 ) .The protein levels of phosphorylated AMPK and Bcl-2 were up-regulated significantly , and the expression of cleaved caspase-3 was down-regulated significantly (P<0.05).The level of miR-33 was decreased significantly (P<0.01).CONCLUSION:Liraglutide alleviates liver injury in type 2 diabetic mice , and the mechanism may be associated with reducing the level of miR-33 and increasing the phosphorylation of AMPK in the liver tissues , thereby inhibiting hepatocyte apoptosis .

Objective To develop a method for the determination of four saponins of panax notoginseng in Compound Dan-shen Prescription (CDP) by HPLC. Methods The conditions for the experiment were: determination of notoginsenoside R1, ginsenoside Rg1 and ginsenoside Re with acetonitrile - 0. 05 % phosphoric acid (19 : 81) as mobile phase and the detection wave at 203nm, determination of ginsenoside Rb1 with acetonitrile - water (33 : 67) as mobile phase and the detection wave at 203nm. Results A good linearity for notoginsenoside R1 was in the range of 0. 24 ~ 3. 6?g ( r = 0. 9997), 1. 2 ~ 18?g (r = 0. 9994) for ginsenoside Rg1, 0. 08 ~ 1. 2?g(r = 0. 9997) for ginsenoside Re and 0. 25-3. 72?g (r = 0. 9998) for ginsenoside Rb1. The stability of the four saponins is well kept at 4℃ for four months. Conclusion This method is simple, rapid, accurate and with good reproducibility and can be used for the quality control of CDP.

Objective To observe the effect of Huangqi Danshen Drop Pill(HDDP) on pituitary (Pit) induced acute myocardium ischemia. Methods Acute myocardium ischemia rat model was established by sublingual venous injection of Pit(0. 35U/kg). The changes of the T wave ( II channel ECG) and serum contents of lactate dehydrogenase (LDH), CK, superoxide dismutase (SOD) and malondialdehyde (MDA) were observed in various times after Pit injection. Results HDDP could significantly lower the height of T waves, increase SOD activity and decrease MDA content. Conclusion HDDP can improve acute myocardium ischemia induced by Pit.

OBJECTIVE:To observe the therapeutic effect of Tanreqing injection in combination with Piperacillin sodium and Amikacin sulfate for hospital-acquired pneumonia.METHODS:104 cases of hospital-acquired pneumonia were enrolled and randomly assigned to receive Tanreqing Injection+Piperacillin Sodium+Amikacin Sulfate(treatment group,n=52) or Piperacillin Sodium+Amikacin sulfate(control group,n=52) for 7～10days.The clinical indexes including the total effective effect,cardinal symptoms,physical signs,and chest X-ray film in two groups after treatment were observed.RESULTS:The total effective rate in the treatment group was 86.54% as compared 67.31% in control group(P

This study aimed at evaluating the effect of different processing methods on the contents of antiosteoporotic compositions in P.corylifolia.HPLC method adopted to analyze the contents of antiosteoporotic compositions,including coumarin (psoralen and isopsoralen),flavonoids (neobavaisoflavone,corylifolin,corylifolinin and corylifol A) and meroterpenes (bakuchiol) in P.corylifolia.The contents were analyzed before and after the processing methods.Results:The results showed that the content of coumarin,flavonoids and meroterpenes obviously changed in different processed P.Corylifolia in comparison with the crude drugs.In conclusion,it was demonstrated that the therapeutic efficacy of osteoporosis was enhanced by CP method which increased all of the three compositions.The testimony tallied with the Chinese medical theory:stir-frying with salt-water could enhance the effect of the tonification of spleen and kidney.

Objective:To study the effects of PRF and recombinant hPDGF-AB,TGF-β1 and VEGF on the proliferation and adhe-sion of rat adipose tissue-derived stem cells(ADSCs)in vitro.Methods:ADSCs were cultured with PRF membrane and various do-ses of PDGF-AB,TGF-β1 and VEGF,cell adhesion was examined by adhesion assay after 2 culture,cell proliferation was examined by CCK-8 kit after 1 -7 d culture.Results:Cell adhesion assay showed that the adhesive numbers of rat ADSCs in PRF group were significantly higher than those in the negative group(P <0.05).The adhesive numbers of the ADSCs treated by PDGF-AB showed no significantly difference among different concentration groups(P >0.05).The adhesive numbers of the ADSCs treated by VEGF or TGF-β1 at different concentrations showed significant difference(P <0.05).CCK-8 kit assay showed that at different time points, the A values of ADSCs in PRF group were significantly higher than those of the negative control group(P <0.05).The A values of ADSCs in VEGF or PDGF-AB groups at different concentrations showed significant difference(P <0.05).The A values of rat AD-SCs in TGF-β1 group at different concentrations were lower than those in the negative control group(P <0.05).Conclusion:PRF as a combination of growth factors may stimulate the proliferation and adhesion of rat ADSCs in vitro.PDGF-AB and VEGF may stim-ulate the proliferation of rat ADSCs.TGF-β1 and VEGF may stimulate the adhesion of rat ADSCs in a dose-response manner to some degree.

Objective To evaluate the quality of Psoralea corylifolia collected from 12 provinces of China.Methods An HPLCDAD-MS/MS method was used to identify,determine,and estimate 14 representative bioactive compounds in P.corylifolia.Then on the basis of the content data,the chemometrics method was used to differentiate 20 samples from different regions.Results The quality of P.corylifolia from 12 different provinces of China was evaluated by this method.Though the samples showed similar profiles,content of the detected markers varied significantly in different regions and batches.According to the results of the hierarchical cluster analysis and principle component analysis,it can be concluded that the samples from different origins could be clustered reasonably into two groups,as well as successfully distinguished.Conclusion A simple and reliable new method which used HPLC-DAD-MS/MS and chemometrics has been developed to characterize,classify,and control the quality of P.corylifolia.