1.The Theories of Yi Xue in the Yellow Emperor's Classic of Medicine
International Journal of Traditional Chinese Medicine 2008;30(3):196,200-
The Book of Changes is known as a famous ancient book in China. In the chapter on Copula of this masterpiece the relations among the changes of heaven and earth, movement and stillness, and Qian and Qun have been revealed through adopting "YiXing" and "LiYi", two varieties of schools of Yi (Change). This paper sets out to compare the depiction of Yin and Yang, movement and stillness recorded in the book of the Yellow Emperor's Classic of Medicine with the notions mentioned above. It is also our attempt to discover the same intrinsic principle existed in both Chinese ancient philosophy and Chinese ancient medicine.
2.Design of Balance Function Telerehabilitation System Based on C/S.
Xiulin XU ; Jiaojiao ZHANG ; Meijun AN ; Jianhui WANG
Journal of Biomedical Engineering 2015;32(2):363-372
This article shows a new design of telerehabilitation system for balance function assessment and training in our laboratory. The system is based on C/S network architecture, and realizes the telecommunication through socket network communication technology. It implements the teletransmission of training data and assessment report of sit-down and stand-up, online communication between doctors and patients, and doctors'management of patient information. This system realizes remote evaluation and telerehabilitation of patients, and brings great convenience for the patients.
Humans
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Physical Therapy Modalities
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Postural Balance
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Rehabilitation
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Telecommunications
3.Development of Lower Limbs Rehabilitative Training Robot (review)
Jiaojiao ZHANG ; Xiufang HU ; Xiulin XU
Chinese Journal of Rehabilitation Theory and Practice 2012;18(8):728-730
The paper overviewed the research progress of the lower limb rehabilitation training robot at home and abroad, introduced some kinds of representative lower limb rehabilitation training robots, and put forward the prospects of rehabilitation training robot
4.Performance Verification of XN-1000 Hematology Analyzer
Linyun LI ; Xiulin XIAO ; Changzheng WANG ; Juanan ZHANG
Journal of Modern Laboratory Medicine 2015;(3):126-128,132
Objective To verify the main index of Sysmex XN-1000 hematology analyzer and check whether performance of XN-1000 hematology analyzer meet the quality requirement.Methods The background value,carryover rate,precision,line-arity,reportable range,accuracy and comparability of different modes of sample absorbing of Sysmex XN-1000 hematology analyzer were verified and WS/T 406-2012 Analytical Quality Specifications for Routine Tests in Clinical Hematology was referred.Results The background counts,carryover rate,inter-batch precision and inter-day precision all met the require-ment.Linearity verification of WBC,RBC,HGB and PLT all met the requirement of the value of a within 1±0.05 and the correlation r≥0.975.The reportable range surpassed the range stated by the manufacture,which was accepted as the clinic reportable range.The accuracy test showed the mean and bias all met the requirement.The difference between the values de-tected by different absorbing modes all met the comparability requirement.Conclusion Performance of Sysmex XN-1000 he-matology analyzer was verified to meet the requirement.
5.Study of morphological and immunohistochemical characteristics of phyllodes tumors
Yongjun YANG ; Xiulin ZHANG ; Zuojun WANG ; Qingmin TAN
Cancer Research and Clinic 2014;26(1):32-35
Objective To investigate the morphological and immunophenotypic characteristics of phyllodes tumors (PT) and explore their significance in differential diagnosis and evaluation of prognosis.Methods 48 specimens with PT were observed under light microscope with HE and immunohistochemical staining (EnVision method).The antibodies including CD34,CD10,CD117,p53,and Ki-67 were used.10 cases of fibroadenoma were compared and the clinical data and follow-up results were analyzed retrospectively.Results All of 48 cases with PT presented as well-circumscribed masses with typically leaflike structures composed of double-layered epithelial component arranged in clefts and overgrowing hypercellular mesenchymal component.31 benign,12 borderline,and 5 malignant PT were diagnosed based on stromal overgrowth,cellular pleomorphism,mitosis,margins,and others.Focal necrosis was detected in 3 malignant cases and liposarcoma component existed in 1 case.Immunohistochemical staining showed that the positive rates of CD34 were 93.5 %,58.3 %,0 in benign,bordline,and malignant PT respectively,there were significant differences between each groups (P < 0.05).The expression of CD10 were 25.8 %,83.3 %,80.0 %,its expression in benign group was significantly different compared with bordline and malignant PT (P < 0.05).p53 was expressed in three groups with no significant difference (P > 0.05).CD117 and Ki-67 had high expression comparatively in malignant PT.All cases were treated surgically with a local recurrence rate of 22.9 %.Conclusions Reasonable surgery pattern based on accurate histopathological diagnosis is crucial to reduce the local recurrence rate of PT.Immunohistochemistry examination in PT is helpful to the differential diagnosis and evaluation of prognosis.
6.Effects of Xuebijing Injection on the Cardiac Function and Calcium Ions in Cardiac Myocytes of Rats after Anoxia-induced Cardiac Arrest-Cardiopulmonary Resuscitation(CA-CPR)
Hourong ZHOU ; Qian ZHANG ; Haijian LIU ; Xiulin YANG
China Pharmacy 2007;0(33):-
OBJECTIVE: To investigate the effect of Xuebijing injection on the cardiac function and calcium ions of cardiocyte in rats after anoxia-induced cardiac arrest-cardiopulmonary resuscitation(CA-CPR).METHODS: CA-CPR model was induced in rats and then the model rats were randomized to 4 groups,i.e.sham group,model group,Xuebijing injection high dose group(4 mL?kg-1),and Xuebijing injection lowdose group(Xuebijing injection 2 mL?kg-1).Mean arterial pressure(MAP),?LVdp/dt max,average fluorescence intensity of calcium and pathological changes of cardiocytes were observed at 0 and 6 hours after resuscitation,respectively.RESULTS: Compared with model group,Xuebijing ingection high dose group at 6 h showed significantly increased MAP(P
7.Differences of glucose metabolism between adipose tissue and skeletal muscle in the early stage of catch-up growth in rats and its mechanism
Lulu CHEN ; Xiaofen WANG ; Juan ZHENG ; Xiulin DENG ; Jiaoyue ZHANG ; Tianshu ZENG
Chinese Journal of Endocrinology and Metabolism 2008;24(3):244-247
Objective To observe the glucose utilization in adipose tissue and skeletal muscle during catch-up growth, and to explore the mechanism of catch-up growth of adipose tissue. Methods Male Wistar rats were randomly divide into normal control group(NC)and catch-up group(RN). Rats in RN group received 50%of food consumed by NC group for 4 weeks, then were re-fed spontaneously as the rats in NC group. In the end of the fifth week(NC1 group and RN1 group)and the sixth week(NC2 group and RN2 group), the experiment was performed. [3]-2-deoxy-glucose was used for detecting the glucose uptake rate. RT-PCR and Western-blot were used for detecting the levels of mRNA and membrane protein of glucose transporter-4(Glut4). Results The glucose uptake rates in adipose tissue and skeletal muscle of RN1 group increased by 189.6%(P<0.01)and reduced by 36.5%(P<0.05)respectively, as compared with NC1 group. After 2 weeks of catch-up growth, the glucose uptake rates in adipose tissue of RN2 group increased by 157.3%(P<0.01)and decreased by 41.5%(P<0.05) in skeletal muscle as compared with NC2 group. However. no significant difference in Glut4 mRNA levels in muscle or in adipose tissue between NC and RN groups were found. The membrane protein of GIut4 after insulin-stimulating in RN1 group and RN2 group reduced by about 46.5%(P<0.01)and 32.1%(P<0.05)in muscle and increased by 116.5%(P<0.01)and 89.9%(P<0.01)in adipose tissue respectively. Conclusion There exists the redistribution of glucose from skeletal muscle to adipose tissue during the early stage of catch-up growth, which results in the catch-up growth of adipose tissue. This change is induced by the tissue-specific alteration of insulin-stimulated Glut4 protein translocation.
8.Clinical significance of serum B7-H4 protein levels in epithelial ovarian cancer
Ping ZHAO ; Xingjian CAO ; Xiulin ZHANG ; Chao YU ; Xiaoyan LU ; Yongmei WANG
Chinese Journal of General Practitioners 2011;10(4):260-262
Serum B7-H4 protein levels were measured by enzyme-link immunosorbent assay (ELISA) in 62 patients with epithelial ovarian cancer, 20 patients with benign ovarian diseases, and 30healthy subjects.Serum B7-H4 protein levels in epithelial ovarian cancer, benign ovarian diseases and normal controls were 0.72(0.32,1.88 )μg/L, (0.45±0.21)μg/L,(0.38 ± 0.19 ) μg/L respectively (P<0.01).The serum level of B7-H4 protein in epithelial ovarian carcinoma was correlated with FIGO stages, lymph node metastasis, histological grade and histological types ( all P < 0.01 ), but not with menopause and ascites (P>0.05).The receiver operating characteristics curve analysis revealed that B7-H4protein had a sensitivity and specificity of 69% (43/62)and 86%(43/50) for diagnosis of epithelial ovarian cancer.
9.Construction and Identification for Over-expressing Lentiviral Vector of mSema3A
Xiulin YAN ; Yuwen CHEN ; Jie JIN ; Yu ZHU ; Jian WANG ; Yang ZHANG ; Li LU
Journal of China Medical University 2015;(3):226-229,233
Objective To construct and identify over?expressing lentiviral vector of mSema3A. Methods Sema3A gene of mice was amplified by PCR,then the gene was inserted into plasmids pDown?mSema3A?IRES/EGFPby Gateway technology. The plasmids pLV/EXPNZ?puro?mSema3A?IRES/EGFP were produced by recombination. After sequencing identification,the vector pLV(Exp)?Puro?CMV?mSema3A?IRES/EGFP was packed and condensed. Finally the recombinant vectors were used to transfect 293T cells to obtain virus pools. Results The recombinant lentiviral vectors were 11 538 bp with EGFP marker,and Sema3Agenes were inserted into the lentiviral vector correctly,indicating the over?expressing vector of Sema3A gene in mice was successfully constructed. Conclusion The over?expressing lentiviral vector of mSema3A was constructed correctly, which lay a foundation of screening of over?expressing strains of such gene in specific cells.
10.The analyse on unquanlified screening results of blood donors′ infectious markers in Chongqing City
Xiulin HUANG ; Dan YIN ; Leijing BI ; Qiaolin ZHANG ; Ming LEI ; Wei LI
Chongqing Medicine 2016;(2):236-237,240
Objective To investigate the unquanlified screening results of blood donors′ infectious markers in this center ,de-velop a scientific blood screening policy and provide a basis for assessing the efficiency of blood screening reagent .Methods un-quanlified screening results of blood donors′ infectious markers in this center were analyzed from July 2014 to June 2015 ,and the distribution of detection reagents were also detected .Results 120 756 samples were detected in Chongqing blood center ;among 2 854 cases of unquanlified samples ,there were 768 cases of ELISA + /NAT + ,38 cases of NAT + /ELISA - 3 (111 cases NAT were i-dentified as HBV) ;unqualified specimens of anti-TP ,HBsAg ,anti-HIV ,anti-HCV were 895 ,1 012 ,276 and 444 cases respectively ;Double ELISA reagent unqualified rate were 78 .6% ,77 .3% ,30 .8% ,26 .1% respectively .The main unqualified results of NAT were HBV ,the blood donors that were reactive in only HBsAg single reagent of ELISA also reactive for HBV in differential NAT . Conclusion On the condition that comply with laws and operations specification ,the blood screening strategy of selecting once ELISA and once NAT rationally is feasible .