Objective To study the effect of manganese chloride on apoptosis,p53 and Bcl-2 expression in the spermatogenic cells of rats.Methods 24 healthy male SD rats were randomly divided into three groups.Two groups were treated with MnCl2?4H2O through intraperitoneal injection at 15 mg/kg and 30 mg/kg respectively,once a day and 5 times in a week for four weeks,the third group served as the control and given normal saline.The spermatogenic cell apoptosis was examined by transmission electronic microscope and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling(TUNEL)technique.Expression of p53 and Bcl-2 were measured with immunohistochemistry.Results The apoptosis index(AI)and p53-positive-cell rate in manganese exposed group were significantly higher than those in the control group(P

Objective To study the antagonism of glutathione(GSH) to antioxidative capacity decrease induced by manganese exposure in rats.Methods 48 healthy male SD rats were randomly divided into six groups: the blank control,GSH control,MnCl2.4H2O(15 mg/kgand 30 mg/kg),15 mg/kgMnCl2.4H2O+1 mmol/kgGSH and 30 mg/kg MnCl2.4H2O+1 mmol/kg GSH,the treatment was conducted through peritoneal injection.The activity of superoxide dismutase(SOD),catalase(CAT) and glutathione peroxidase(GSH-Px)in the serumand the testis were determined bycolorimetric analysis.Results Compared with the blank control group,the activity of SOD,CAT and GSH-Px in the serum and testis in 30 mg/kg groups significantly decreased(P

Objective To study the effects of manganese on the expression of caspase-3 and cytochrome-c in the spermatogenic cells of rats. Methods Forty-eight male SD rats were randomly divided into the high dose,low dose exposure groups and the control groups,16 in each,were given manganese(MnCl2?4H2O) at 15,30 mg/kg and normal saline,I.P. once a day, 5 times a week,for 6 consecutive weeks. In the 4th and 6th weekend,the testis were collected,the expression of caspase-3 and cytochrome-c in the testis was determined by immunohistochemical methods (SABC). Results The caspase-3-positive-cell rate in the spermatogenic cells of rats in the high dose and low dose exposure groups increased significantly (P