Establishing a teaching quality control system and strengthening the supervision to the teaching quality is an important means and guarantee of the constant improvement and perfection of the quality of teaching at affiliated hospitals

ObjectiveTo investigate the clinical efficiency of oral warfarin after cardiac valve replacement and the influence of vitamin K epoxide reductase complex subunit 1(VKORC1) gene polymorphisms on warfarin maintenance dose.MethodsOne hundred and fifty-nine patients who got cardiac valve replacement surgery were chosen and received anticoagulation therapy by oral warfarin.The prothrombin time (PT),international normalized ratio (INR) of patients were recorded and the safety ranges of PT and INR were calculated statistically.VKORC1 gene polymorphism of patients weredetected by PCR-PFLP technology by adjusting the dose of warfarin,and the results were compared.ResultsThe safety monitoring range of PT of oral warfarin after cardiac valve replacement surgery was 15.36 -24.82 s,safety monitoring range of INR was 1.33 - 2.62.The occurrence rate of bleeding during anticoagulation was 13.21％(21/159).The weekly dose of warfarin of VKORC1 gene type AA [(24.28 ± 10.79) mg] was significantly higher than that of VKORC1 gene type GA[ ( 16.64 ± 7.43 ) mg] and type GG[ ( 12.12 ± 7.17 ) mg](P＜ 0.05or ＜0.01 ).ConclusionsThe polymorphism of VKORC1 gene is the dominant factor of the differences of warfarin maintenance dose.The warfarin dose in patients with different gene type is different.The clinical safety monitoring ranges of PT and INR in patients with oral warfarin after cardiac valve replacement are lower than the recommended ranges of European and American countries.Therefore,the index of patients after surgery should be detected regularly.

0.05). Conclusion The movement of train has not obviously affect to the routine blood test.

For the past few years,with the extensive application of nanoparticle technology in photodynamic therapy (PDT) of cancers,there have been some improvements of the stability of photosensitizers,the tumor targeting,the working depth and the yield of singlet oxygen by a variety of ways.Furthermore,with nanoparticle,PDT also can be combined with some other treatments together.Finally,the use of nanoparticle vectors for PDT is a novel and promising approach which might improve the efficiency of photodynamic therapy for tumors,overcome many adverse effects and increase the indications of PDT.

Objective To evaluate the effect of 5-aminolevulinic acid-based photodynamic therapy (ALA-PDT) on S.epidermidis biofilms.Methods S.epidermidis was cultured on cover glasses,with or without thepresence of ALA at 50 mmol/L for 16 hours followed by the exposure to different doses (100,200,300 J/cm2) of red light.S.epidermidis receiving neither pretreatment nor irradiation served as the negative control.Subsequently,confocal laser scanning microscopy (CLSM) was used to observe the structure and evaluate the biological activity of S.epidermidis biofilms; colony forming units (CFUs) of S.epidermidis were counted for the evaluation of killing effect of ALA-PDT; scanning electron microscopy (SEM) was used to observe the morphological structure of the biofilms.Results The number of colony forming units per milliliter (CFU/ml) was (210 ± 7.55) × 105,(91 ± 1.53) × 105,(16 ± 1.52) × 105 for S.epidermidis pretreated with ALA followed by irradiation with red light at 100,200 and 300 J/cm2 respectively,significantly different from untreated S.epidermidis ((388 ±8.89) × 105,all P ＜ 0.01) and S.epidermidis irradiated with red light only.Increased ratio of dead to live cells was observed in ALA-pretreated S.epidermidis irradiated with red light at 100,200 and 300 J/cm2compared with untreated S.epidermidis (1.254 ± 0.096,1.301 ± 0.160 and 3.410 ± 1.140 vs.0.358 ± 0.057,all P ＜ 0.01) and S.epidermidis irradiated with red light only.SEM showed that the biofilm structure was loose and obscure in S.epidermidis treated with ALA-PDT,and even disappeared with S.epidermidis distributed in single colonies when the dose of red light was 300 mJ/cm2.Conclusion ALA-PDT shows a potential bactericidal activity against S.epidermidis in biofilms,which can not only reduce biofilm vitality,but also damage biofilm structure.

Adult ; Chromosome Aberrations ; drug effects ; Ethylene Oxide ; poisoning ; Humans ; Lymphocytes ; drug effects ; pathology ; Male ; Micronuclei, Chromosome-Defective ; drug effects

ObjectiveTo investigate and analyze the prevalence and risk factors of acute stress disorder (ASD) among survivors of road traffic accidents.MethodsA total of 206 participants ( 168 men,38 women) were administrated with the acute stress disorder scale (ASDS) 2 to 23 days after the traffic accidents.ResultsThere was a total of 61 (29.6％) possible ASD patients among all participants.Women had more serious ASD symptoms than men (male:36.70 ± 11.42; female:41.18 ± 12.58; P＜ 0.05 ),the married's ASD symptom severity was higher than the single's ( married:38.37 ± 11.94 ; single/widowed/divorced:30.86 ± 8.37 ; P ＜ 0.01 ),people with low degree of education had more severe ASD symptoms than the people who had high degree of education (P＜0.05) ; being elder( r=0.43,P＜0.01 ),having fear / helpless / horror experience of accident ( r=0.21,P＜ 0.01 ) were significantly correlated with the severity of ASD symptoms.According to linear regression analysis,age could positively predicted ASD symptoms ( β =0.29,P ＜ 0.01 ) too.ConclusionASD is a common mental problems among Chinese survivors of road traffic accident,and the main predictive factors of ASD include gender,age,marital status,the degree of education,whether there is fear / helpless / horror experience of accident.

The DNA microarray study showed that in STZ-induced diabetic rats the elevatedexpression of 42 genes in artery were depressed markedly after atorvastatin treat ment .This suggeststhat atorvastatin may have the protective effects on the diabetic vascular lesion.

Objective To study the characteristics of self-concept and personality in the SOS children vil-lage's children. Methods Sixty-one children from SOS children village, sixty-one children with single parent and sixty-one children in intact family, matched with the SOS children in gender and age, were assessed by Piers-Harris children's self-concept Scale(PHCSS), Eysenck Personality Questionnaire for Children (EPQ) and general status questionnaire. Result In the PHCSS, the scores on behavior, body of color and attribute, blessedness and satisfac-tion and the total score of boys in the SOS group were lower than those in the intact family group's ((10.69± 2.74) vs (12.57±2.36),P=0.013;(6.51±2.63) vs (8.29±2.75), P=0.011;(6.54±1.93) vs (7.97± 1.60), P=0.004; (48.09±10.88) vs (55.86±10.11), P=0.007)). The E scale's score of the SOS group's girls was lower than that in the intact group (P=0.004). Children's self-concept status was related with their learning environment, family structure, parent' s learning expectation, daily communication, personality, number of good friends and academic performance (-0.566 < r < 0.395). Conclusions The self-concept status of girls in the SOS children village show lower level. The personality of boys in the SOS children village tend to be introver-sive and stable. Children's self-concept status was related with the factors of their personality, life environment,family structure,et al.

Objective To investigate the effect of the mascarinic acetylcholine receptor (mAChR) agonist Oxo-tremorine-M (Oxo-M) on glycinergic spontaneous inhibitory postsynaptic currents (sIPSCs) and micro-inhibitory postaynaptic currents (mIPSCs) in lamina Ⅱ neurons in the spinal cord of rats. Methods Glycinergic IPSCs (sIPSCs and mIPSCs) in lamina Ⅱ neurons of spinal slices were recorded using the whole-cell voltage-clamp method. The non-selective mAchR ngonist Oxo-M was applied through bath perfusian. The effects of Oxo-M 1, 3, 5 and 10 μmol/L on sIPSCs and mIPSCs were examined. Results Oxo-M at the concentrations of 3-10 μmol/L significantly increased the frequency of sIPSCs without changing the amplitude in 16 lamina Ⅱ neurons tested. Interestingly, when the concentration of Oxo-M was increased to 10 μmol/L, the potentiating effect of Oxo-M on the frequency of slPSCs was decreased as compared with 3 μmol/L Oxo-M in the above 16 neurons. The slPSCs were completely abolished by 2 μmol/L strychnine. Atropine, the specific mAChR antagonist, completely blocked the effect of Oxo-M on the frequency of sIPSCs. In 9 additional lamina Ⅱ neurons, 1-10 μmol/L oxo-M failed to alter significantly the frequency and amplitude of glycinergic mIPSCs. Conclusion Activation of mAChRs in the somatodendritic site of glycinergic interneurous increases the synaptic glycine input to spinal dorsal horn neurons, but not in a dose-dependent manner.