ObjectiveTo measure levels of serum resistin in patients with nonalcoholic fatty liver disease (NAFLD) and explore the relationship between serum resistin and insulin resistance,and the role of serum resistin in the pathogenesis and progression of NAFLD.Methods 53 patients (including 30males and 23 females) with NAFLD and the 28 control subjects (including 16males and 12females) were enrolled in this study.The height,weight,waist circumstance,hip girth and blood pressure of all subjects were obtained by standard methods.To all the subjects,the parameters including fasting blood glucose (FBG),triglyceride ( TG),total cholesterol (TC),high-density lipoprotein cholesterol ( HDL-c ),lowdensity lipoprotein cholesterol ( LDL-c ),alanine aminotransferase ( ALT),aspartate aminotransferase (AST)and gamma glutamyl transferase (GGT) were measured.Serum resistin was measured by electrochemiluminescence immunoassay (ECLIA) and fasting serum insulin (FINS) were measured by enzymelinked immunosorbent assay (ELISA).Body mass indexes (BMI),waist hip ratio (WHR),body fat percentage ( BF％ ) were calculated with formula.Homeostasis model assessment was applied to assess the status of insulin resistance index (HOMA-IR).The relationships between serum resistin and various parameters in patients with NAFLD were observed and analyzed by statistical methods.ResultsThere were no significant differences in the number of cases,average age,compositions of sex and BF％ between the NAFLD group and the control group ( P ＞ 0.05).In the NAFLD group,systolic blood pressure ( SBP),diastolic blood pressure(DBP).The waist circumference,hip girth and BMI and WHR were higher than those of control group ( t =3.54,3.32,3.56,5.85,5.56,4.10,P =0.001).There were no significant differences on the level of TC,HDL-c,LDL-c between the NAFLD group and the control group ( P ＞0.05).In the NAFLD group,FBG,TG,AST were higher than those of control group ( P =0.001,P =0.004,P =0.001 ).The levels of serum resistin,FINS,HOMA-IR,ALT and GGT in NAFLD group were significantly higher than those of control group,and there was significant difference between two groups ( P =0.0005).There was no significant correlation between serum resistin and age,BMI,WHR,BF％,FBG,TG,TC,HDL-C,LDL-C,AST in NAFLD group ( P ＞ 0.05).The levels of serum resistin in patients with NAFLD were positively correlated with GGT,ALT,FINS,HOMA-IR ( r =0.354,0.391,0.875,0.881,P ＜0.05 or ＜0.01 ).After multiple stepwise regression analysis,the results showed that HOMA-IR was the most important factor for affecting the levels of serum resistin.The levels of serum resistin in male and female patients with NAFLD were higher than those of control group ( P ＜0.05).But no statistical difference was found between males and females in two groups ( P ＞ 0.05).ConclusionsThe levels of serum resistin in patients with NAFLD were significantly higher than those of controls.Serum resistin in patients with NAFLD was closely correlated with insulin resistance,and it may participate in the pathogenetic progress of NAFLD.Serum resistin in patients with NAFLD might be associated with hepatic insulin resistance,and it has no correlations with obesity-related insulin resistance.Serum resistin in patients with NAFLD may participate in the inflammation of fatty liver disease as inflammatory cytokines.

Tumor stem cells have abilities of high proliferation,serf-renewal and multi-directional dif-ferentiation ;which is closely responsible for tumor' s survival and recurrence. A minor portion of myeloma stem cells exist in multiple myeloma. The research of myeloma stem cells' origins, phenotypic analyses, signaling pathways, drug resistance and other biological characteristics will do great efforts to the effective therapy of mul-tiple myeloma.

Objective To investigate the clinical significance of serum lactic dehydrogenase (LDH)alteration in patients with acute leukemia (AL). Methods Serum LDH of 156 AL patients including untreated 63,completely remission (CR) 46 and relapsed 47 were measured by biochemistry analyzer. R banding technique was used for karyotype analysis this three groups were divided according to poor, good and normal prognostic chromsome. The correlation between LDH level with AL periods, chromsome groups, peripheral WBC counts and leukemia subtypes were analysed. Results The LDH level of AL patients untreated (P50 399 U/L) and relapsed (P50 265 U/L) were significantly higher than those CR (P50 153 U/L), P ＜0.05. LDH level were closely correlated with peripheral WBC counts (rs=0.604) and leukemia cells of bone marrow (rs=0.538, both P ＜ 0.01), and both were also correlated with leukemia subtypes (LDH in subtypes L2, M4 were higher than other subtypes of AL, P ＜ 0.01). The refractory and relapsed AL patients had higher LDH level (P50 538 U/L) than the others (P50 294 U/L) when they were diagnosed. And those with poor prognostic chromsome also had higher LDH level (P50 778 U/L) than those with good (P50 306 U/L) and normal prognostic chromsome (P50 405 U/L, P ＜0.01). Conclusion LDH level increased obviously in untreated AL patients and decreased in CR ones. It was correlated with different periods, subtypes and grade malignancy of AL So LDH level can be considered as an important indicator for therapeutic effects and prognosis for AL patients.

Objective To study the characteristics of self-concept and personality in the SOS children vil-lage's children. Methods Sixty-one children from SOS children village, sixty-one children with single parent and sixty-one children in intact family, matched with the SOS children in gender and age, were assessed by Piers-Harris children's self-concept Scale(PHCSS), Eysenck Personality Questionnaire for Children (EPQ) and general status questionnaire. Result In the PHCSS, the scores on behavior, body of color and attribute, blessedness and satisfac-tion and the total score of boys in the SOS group were lower than those in the intact family group's ((10.69± 2.74) vs (12.57±2.36),P=0.013;(6.51±2.63) vs (8.29±2.75), P=0.011;(6.54±1.93) vs (7.97± 1.60), P=0.004; (48.09±10.88) vs (55.86±10.11), P=0.007)). The E scale's score of the SOS group's girls was lower than that in the intact group (P=0.004). Children's self-concept status was related with their learning environment, family structure, parent' s learning expectation, daily communication, personality, number of good friends and academic performance (-0.566 < r < 0.395). Conclusions The self-concept status of girls in the SOS children village show lower level. The personality of boys in the SOS children village tend to be introver-sive and stable. Children's self-concept status was related with the factors of their personality, life environment,family structure,et al.

Objective To study the possible molecular mechanism of the damage of HepG2 cell line induced by low concentration of ethanol and ultraviolet irradiation. Methods Hepatoma cell line (HepG2) was exposed to low concentration of ethanol and ultraviolet irradiation, then the changes in cell cycle, the expression of p53 and p21, the correlation between expression of p53 and p21 and cell cycle were analyzed. Results The expression of p53 and p21 was greatly increased, and the cell cycle was found to be blocked at G_0-G_1/G_2-M after ultraviolet irradiation. The cell cycle block of G_2-M and the increased expression of p21 were observed when exposed to low concentration of ethanol, while the expression of p53 showed no significant changes. Cell apoptosis was increased when the cells were exposed to the both injury factors. Conclusion Low concentration of ethanol and ultraviolet irradiation can affect cell cycle and the process of cell apoptosis through activation of different molecular mechanism.

Objective To investigate the expression of ERBB3 protein,which encoded by ERBB3 gene,in gastric cancer(intestinal type and diffuse type)as well as the normal tissue,and to elucidate the possible role of ERBB3 gene in the progress of gastric cancer.Methods 65 samples of gastric cancer tissue(of which 43 samples were intestinal type and 22 were diffuse type,classified according to the Lauren patho-classification)and 65 samples of normal tissue(control group)were investigated immunohistochemically.The expression of ERBB3 in both control group and gastric cancer group was determined.Comparisons were made according to different pathological types and TNM clinical stages within and between groups.Results 7 of 65 samples(10.8%)in gastric cancer group were found to over-express the ERBB3 protein,while only 1 of 65 samples(1.6%)in control group was found to over-express the ERBB3 protein.Statistical differences(P

This study was to study the effect of cyclosporine A (CsA) on the expression of nitric oxide synthase (NOS) in liver cells in T cell dependent liver injury induced by concanavalin A (ConA) in mice. The results showed that there was no NOS expression in the normal murine hepatocytes. Affer ConA challenge, the expression of NOS in hepatocytes increased markedly.Moreover, the hepatocytes closer to the central vein showed stronger expression.After pretreatment of CsA, activation of T lymphocytes was inhibited and NOS expression in the hepatocytes was almost completely inhibited too.The above results suggest that an upregulation of NOS expression might be a protective response to insufficient supply of blood and oxygen as a result of ConA induced liver injury, and inhibition of the NOS expression by CsA might be associated with inactivation of T lymphocyte.

Objective To observe the change of renal function and cell apoptosis after injecting SB203580 before and after reperfusion, and investigate the protective role of p38 MAPK inhibitor SB203580 for ischemic/reperfused kidney in rats. Methods p38 MAPK inhibitor SB203580 was injected by tail vein into rats with ischemic kidney before and after reperfusion. The plasma levels of creatine and BUN were measured at various time points. The apoptotic rate in the renal tissue at various time points was determined using TUNEL. Results Administering SB203580 before reperfusion could decrease renal cell apoptotic rate, and renal function damage. Administering SB203580 after reperfusion had not obvious effect on the renal function and cell apoptosis. Conclusion Administering p38 MAPK inhibitor before reperfusion can attenuate post-ischemic renal fuction damage and cell apoptosis.

Objective To investigate the changes of intestinal mucosal barrier function and protection of alanyl-glutamine(Alm-Gln) dipeptide during early stage of postthoracotomy in aged patients.Methods A prospective,randomized and controlled trial was conducted and 20 aged patients who underwent non-digestive thoracotomy were randomized into two groups,experimental group (intravenous administration of Aln-Gln dipeptide,0.5 g/(kg · d),for 4 days,n =10) and control group(equal amount saline as placebo,for 4 days,n =10).The indices of boby temperatures,heart rates,respiration and white blood cell count of all patients were daily recorded during administration.Serum concentrations of glutamine (Gln),D-lactate,diamine oxidase (DAO) and tumor necrosis factor-α(TNF-α) were measured before and after operation.Results There were no statistically significant differences in the patients' general information between experimental group and control group including age,gender and body weight.Plasma Gln concentration in postoperative 5 days was higher than that of pre-operation of experiment group ((478.32 ± 47.42) μmol/L vs.(372.67 ± 29.14) μmol/L,P =0.021).The plasma Gln level of control group at 5th day after operation was higher than that in pre-operation ((431.12 ± 42.27) μmol/L vs.(386.29 ± 19.73) μmol/L,P =0.017).The plasma level of Gln in experimental group was significantly higher than that in control group after operation((478.32±47.42) μmol/L vs.(386.29 ± 19.73) μmol/L,P =0.012).There were no significantly differences between the two groups in terms of the plasma level of DAO and D-lactate before operation (P ＞ 0.05).Meanwhile the levels of DAO and D-lactate in both group at 5th day after operation were significantly higher than that at before operation(DAO:(2.53 ±0.47) U/ml vs.(1.66±0.32) U/ml,P =0.003;D-lactate:(6.82 ±1.91) mg/L vs.(4.92 ±1.57) mg/L,P =0.024),and the levels of them in experimental group were significantly lower than that in control group(DAO:(1.10 ± 0.23) U/ml vs.(2.53 ± 0.47) U/ml,P =0.013 ; D-lactate:(4.87 ± 1.33) mg/L vs.(6.82 ± 1.91) mg/L,P =0.019).The concentration of TNF-α was significant increase in both two groups at first day after operation,but decreased at the third day.The concentration of TNF-α in experimental group at 5th day after operation was lower than that in control group ((6.89 ± 5.21) pg/L vs.(13.04 ± 4.46) pg,/L,P =0.003).The morbidity of systemic inflammatory response syndrome (SIRS) was significantly decreased in experimental group and the rate of SIRS was also lower than that in the control group(P ＜ 0.01).Conclusion Intestinal mucosal barrier function was damaged after thoracotomy in aged patients.Administration of Aln-Gln dipeptide could increase the level of serum Gln,protect the intestinal barrier and attenuate the systemic inflammatory response.Aln-Gln dipeptide can be used to help aged patients recover rapidly.

Objective To investigate couple's personality similarity effect on the marital quality,and to explore the vairance of such effect under different levels of spouse's depression. Methods The cross?sectional da?ta of 4644 couple from the national survey of mental health database in china( 2009) was used. Couples completed Locke ?Wallance marital adjustment test,The CES?D scale a self?report depression scale and Eysenck personality questionnaire short scale for Chinese version (EPQ?RSC). Results The personality similarities of couples(0.44 ±0.27) and their marital quality (wife (103.71±27.54),husband (106.15±26.94))had significant positive corre?lation ( r=0.25~0.27, P<0.01). Regression analysis showed the effect of personality similarity on the marital quality were varied according to the level of spouse depression( β=0.09?0.22, P<0.05) . Among participants whose husband or wife had higher level of depression,couple's personality similarity had stronger effect on his/her per?ceived marital quality. Conclusion Husband and wife personality similarity is related to marital quality signifi?cantly,and the spouse depression plays a significant moderating role in this relationship.