In recent years,premature nutritional problems is newborn pediatric paramount concenrn problem.For three material(glucose,protein and fat)metabolism and utilization.premature infants has its own characteristics.In order to avoid side effect,classic parenteral nutrition plan provide for fat emulsion and amino acid gradually,but make premature infants in poor nutrition state.Recent clinical studies have shown that early nutrition is particularly important.Premature infants bom in 24 hours has been given full amount vein nutrition could provide good basis of energy.and avoid associated complications.

New nano-fluorescent quantum dots (QD) have excellent optical properties, enabling them widely used in clinical cancer research. When combined with a variety of ligands, quantum dots have been successfully used for early detection of primary tumor, angiogenesis and metastasis, all of which have opened up a new frontier in early diagnosis of cancer.

Objective To study the influence of pine-seed oil upon body weight,blood lipid and lipid peroxidation of mice fed on high fat feed.Method The mice were fed by high fat feed(80% basal feed added 20% lard,HF) and HF added pine-seed oil(80% high fat feed added 20% pine-seed oil,SHF).At 45 d and 90 d,the body fat content,blood lipid level,and malonyldehyde(MDA),lipofusin(LP),catalase(CAT),superoxide dismutase(SOD) and glutathione peroxidase(GSH-Px) of brain and liver homogenate were measured respectively.Results Pine-seed oil could reduce the body weight serum triglyceride(TG),total cholesterol(TC),low density lipoprotein-cholesterol(LDL-C) content,but increase high density lipoprotein-cholesterol(HDL-C) of the mice fed by HF.It suggested that pine-seed oil had definite effect on regulating blood lipid.Compared with normal control(NC) group,the activities of CAT、T-SOD、GSH-Px of brain and liver in HF group were remarkably lower,but MDA and LP remarkably higher.It suggested that HF could promote lipid peroxidation in brain and liver of mice.Compared with HF group,the activites of CAT,T-SOD and GSH-Px of brain and liver in SHF group were remarkably higher,but MDA remarkably lower.Conclusion Pine-seed oil can reduce the body weight,blood lipid,and lipid peroxidation of mice fed by HF.

Objective To study the value of digital molybdenum target mammography in diagnosing breast cancer.Methods Digital molybdenum target X-ray findings of 36 cases with breast cancer confirmed by pathology were retrospectively analysed.Results X-ray appeared as masses in 22 cases(61.1%),masses with calcification in 7(19.4%),asymmetry increased density with structural disorder in 4(11.1%),Pagets disease in 1(2.7%),pure calcification in 3(8.33%),abnormal vessels in 3(8.33%),skin thickening in 4(8.73%).Conclusion The direct and indirect digital molybdenum target mammographic signs of breast cancer are important for early diagnosis and differential diagnosis of breast cancer.

Objective To observe the clinical efficacy of electrothermal needle apparatus in treating mild hip osteoarthritis.Method Ninety patients were divided into a control group and a treatment group by using the random number table, 45 cases in each group. The treatment group was intervened byelectrothermal needleapparatus; the control group was treated with ordinary electroacupuncture plus TDP radiation. After 15-day treatment, the therapeutic efficacies of the two groups were evaluated by using Visual Analogue Scale (VAS) and Japanese Orthopaedic Association (JOA) scores.Result The VAS and JOA scores were improved inthe two groups after the treatment (P<0.05), and the improvements in the treatment group were more significant than those in the control group after the 15-day treatment (P<0.05).Conclusion Electrothermal needle apparatus can produce a significant therapeutic efficacy in treating mild hip osteoarthritis.

Objective Clinical reports on perilymphatic fistulae(PLF) of the round window membrane(RWM) have shown different degrees of hearing loss. For the unknown mechanisms of hearing loss, the morphological changes of cochlea with perilymphatic fistulae(PLF) was studied.Methods Among 10 guinea pigs, each ear on one side was punctured and the ear of another side was used as control. 4 hours after operation, the morphological changes of cochlea were observed.Results It was found that 2 ears with endolymphatic hydrops, 1 ear with strica vascularis stripped, venous hyperemia and hemorrhage in cochlea, 7 ears with no changes.Conclusion The study suggestes that the normal and the abnormal morphological changes of cochlea can all be seen after the rupture of RWM.

Objective To evaluate the effects of different optional time leading to complication and prognosis by laparoscopic cholecystectomy.Methods Search all of clinical randomized trials on early and delay laparoscopic cholecystectomy in CNKI database.The RevMan 5.0 software was used to conduct the Meta-analysis,which was carried out by collecting information based on inclusion and exclusion criteria from all trails' information available,and the pubhcation bias analysis and the susceptivity analysis was also evaluated to make sure the results stable.Results There was significant statistical difference in conversion,surgery time,complication and postoperative hospitahzation time between the ＜ 72 h group and ＞ 72 h group (P ＜ 0.05).Conclusions Meta-analysis shows that in the laparoscopic cholecystectomy progress,the surgical outcome is best with 72h,which can short the surgery and postoperative hospitalization time,reduce the complication and decline the conversion.

OBJECTIVE To investigate the tinnitus' prevalence in Healthy check-up population in Dalian City. METHODS 1748 cases (male 806, female 942) were chosen from the Physical Examination Center of our hospital from February 1st, 2015 to April 1st, 2015, the tinnitus questionnaire was performed. For the cases with the tinnitus symptoms, the characteristics of tinnitus were further inquired, including the course of tinnitus, seizure frequency, the duration of tinnitus, pulsatile or persistent, unilateral or bilateral. RESULTS The total prevalence of tinnitus is 32.4%(566/1748), the prevalence of persistent tinnitus is 18.3%(320/1748), and the transient tinnitus is 14.1%(246/1748); The prevalence of tinnitus in female are 36.4%(343/942), 20.0%(188/942) and 16.5%(155/942) in turn, which are higher than that of the male 27.7%(223/806), P<0.01; 16.4%(132/806), P =0.054, and 11.3%(91/806), P<0.01. Both the total and persistent tinnitus prevalence increase with age(P<0.01), but the transient tinnitus doesn't increase with age. The prevalence of objective and subjective tinnitus are 1.3%(6.9% out of total prevalence of tinnitus), and 17.0%(93.1% out of total prevalence of tinnitus), respectively. The rate of bilateral tinnitus is 78.1% out of total tinnitus, and rate of unilateral tinnitus is 21.9%(right 11.6%, left 10.3%). In addition, the rates of acute tinnitus, subacute tinnitus, and chronic tinnitus are 4.4%, 3.8%, and 91.9% out of total, respectively. Otherwise, the rates of sleep disorders and hearing loss complaining accounted for 31.9% and 14.1% respectively. CONCLUSION The prevalence of tinnitus is high in healthy check-up population of Dalian, the total prevalence of tinnitus is 32.4%, mainly bilateral,chronic and subjective.

BACKGROUND:Both in vivo and vitro microenvironment can influence the proliferation and differentiation of neural stem cells (NSCs). In addition, cell culture solution plays variable roles in cell proliferation and differentiation.OBJECTIVE:To develop a convenient and rapid method to promote NSC primary culture by modifying traditional serum-free medium. DESIGN, TIME AND SETTING:Randomized controlled cell trial was performed at Institute of Brain Science, Qingdao University Medical School from November 2005 to September 2006.MATERIALS:Ten Wistar rat of gestation for 12-16 days; cell suspension fron brain tissue of embryonic rat. METHODS:Cell suspension were seeded into four 50-mL culture flasks with cell density of 1×106 mL-1, and divided into 2 groups with 2 flasks in each group. The control cells (flasks A and B) were cultured in serum-free medium containing DMEM/F12, B27 (2%), basic fibroblast growth factor (20 μg/L), and epidermal growth factor (20μg/L), and the experimental cells (flasks C and D) were firstly cultured with DMEM/F12 containing fetal bovine serum (FBS, 5%), following by the same serum-free medium 2 to 3 days later. MAIN OUTCOME MEASURES:The proliferation of NSCs was observed by inverted microscopy and immunocytochemistry.RESULTS:①Most of the cells in two culture conditions expressed nestin, the specific marker of NSCs, and were immunocytochemically positive. ②Neural stem cells cultured with FBS formed neurospheres 3-4 days earlier than those without FBS. ③There were no significant differences in cell number, but the neurospheres under the experimental condition were larger than those in control group. Some of the cells were neurone specific enolase-positive after serum-conditioned induction, and some were glial fibrillary acidic protein-positive, indicating NSCs had differentiated into neuron-like cells and neurogliocytes.CONCLUSION:FBS-conditioned pre-culture can accelerate the proliferation of neural stem cells.

Objective To explore the clinical value of the serum cystatin C (Cys C) changes in different periods of the infantile pneumonia.Methods Ninety-two cases of infantile pneumonia (59 mild pneumonia patients and 33 severe pneumonia patients) from November 2012 to March 2013 were collected and 40 cases of healthy infant were enrolled for control group.The levels of serum Cys C,urea and creatinine in acute and recovery period were detected.Results In severe pneumonia patients,the average level of Cys C in acute period [(1.98±0.33) mg/L] is significantly higher than that in control group [(0.85 ±0.24) mg/L] (P ＜0.01),and there was no significant difference between recovery period [(1.12 ± 0.23) mg/L] and control group (P ＞ 0.05),and there was significant difference between acute and recovery period (P ＜0.05).In mild pneumonia patients,there were no significant differences in the level of Cys C between the acute period [(1.10 ±0.22) mg/L] and recovery period [(0.94 ±0.21) mg/L] as well as control group (P ＞ 0.05).The positive rate of Cys C in severe pneumonia patients (51.9％,14/27) was higher than that of urea and creatinine (3.7％,1/27) (P ＜0.01).Conclusion Severe pneumonia could result in renal dysfunction,which is reversible.Cys C can be the clinical reference for early diagnosis and the therapeutic effect in severe pneumonia patients with renal dysfunction.