Objective To investigate the clinical effects of treatment of chronic pulmonary heart disease (CPHD) at acute stage with sodium ferulate and Ciwujia injection. Methods 90 cases of CPHD at acute stage were recruited into treatment group 1, treatment group 2 and control group. The control group was treated with consistent inhalation of oxygen,antibiotics, and symptomatic therapies like relieving cough, removing sputum, and stopping wheeze. The treatment group 1was treated with sodium ferulate, Ⅳ 0.2g, once daily for successive 14days, in addition to the conventional treatment. The treatment group 2 was treated with Ciwujia Injection, 100ml Ⅳ, once daily for successive 14 days. Observe the curative effect and the changes of blood gas analysis indexes (PAo2, PaCO2, SAO2) and bemorheological indexes (whole blood high-cutting viscosity, whole blood low-cutting viscosity, whole blood recovery viscosity, erythrocyte aggregation index, and HCT) before and after treatment. Result Compared with the control group, both treatment groups got improvement in blood gas analysis indexes and hemorheological indexes and curative effect, but no significant difference between the two groups (P＞0.05).Conclusion both sodium ferulate and Ciwujia injection had good results in treating CPHD at acute stage, and there was no difference between the two medicines in terms of curative effects.

T cells redirected to specific antigen targets with engineered chimeric antigen receptors (CARs) are emerging as powerful therapies in hematologic malignancies.Various CAR designs,manufacturing processes,and study populations,among other variables,have been studied and reported in clinical trials.The accumulating data supporting CAR-T cells therapy for disease such as chronic lymphocytic leukemia (CLL)and acute lymphocytic leukemia (ALL) highlight what may well become the next sea change in the care of patients with all types of hematologic neoplasia.The scientific symposium on CAR-T cells therapy inspires the mounting enthusiasm regarding this new treatment strategy.Here,the results of the reported clinical trials and the outlook for CAR-T cells therapies were reviewed and discussed.Many questions remain in the field of CAR-T cells directed to hematologic malignancies,but the encouraging response rates pave a wide road for future investigation.

BACKGROUND:Bone marrow mesenchymal stem cel s can be differentiated into myocardial cel s induced by 5-azacytidine in vitro, which provides an opportunity for cel transplantation in the treatment of heart disease. OBJECTIVE:To study the induction effects of 5-azacytidine at different concentrations on the myocardial differentiation of bone marrow mesenchymal stem cel s in rats. METHODS:Passage 3 bone marrow mesechymal stem cel s were incubated with DMEM containing 0, 5, 10, 20μmol/L 5-azacytidine for 24 hours, and then the induced medium was replaced by DMEM containing 5%fetal bovine serum for subsequent 28-day culture. Afterwards, expression of cardiac troponin I was detected by immunocytochemical method. RESULTS AND CONCLUSION:Cel death occurred at 24 hours after 5-azacytidine induction, which was more obvious in the 20μmol/L group than the 5 and 10μmol/L groups. The positive expression of cardiac troponin I was significantly lower in the 5μmol/L group than the 10 and 20μmol/L groups (P<0.05), but there was no significant difference between the 10 and 20μmol/L groups (P>0.05). These experimental findings indicate that 5-azacytidine can induce the differentiation of rat bone marrow mesenchymal stem cel s into myocardial cel s in vitro, and its optimal concentration is 10μmol/L.

Objective To investigate the treatment effect of gonadotropin releasing hormone agonist and oral contraceptive on endometriosis diseases.Methods One hundred and eighty-six cases with endometriosis who were hospitalized in Traditional Chinese Medicine Hospital of Fangshan District of Beijing from Jan.2012 to Dec.2012,were randomly divided into control group (93 cases) and observation group (93 cases).Patients in control group were given Marvelon by oral from the first day of menstruation after operation to 6 months,1 tablet/day,and patients in observation group were given Marvelon combined with Zoladex after laparoscopic conservative treatment (3.6 ml subcutaneous injected Zoladex from the first week after operation,4 weeks/time,3 times).The total subjective symptom scores,efficacy rate,Pregnancy rate and adverse reaction were calculated and recorded.Results Subjective symptom score of observation group was (0.91 ± 0.32),significantly lower than that of the control group (1.64 ± 0.74 ; t =8.73,P ＜ 0.01).The efficiency rate in observation group were 95.70％ (89/93) was significantly higher than that in control group(82.80％ (77/93) ;t =4.328,P ＜0.05).Adverse reactions rate and reoccurrence in observation group were 4.30％ (4/93) and 3.23％ (3/93),lower than that of the control group(16.13％ (15/93) and 10.75％ (10/93) ; x2 =4.854,5.168 ; P ＜ 0.05).After treatment,the pregnancy rate in infertile patients of observation group the was 85.71％ (12/14),significantly higher than that of the control group (61.54％ (8/13),x2 =3.987,P ＜ 0.05).Conclusion Gonadotropinreleasing hormone agonist combined contraceptive is worthy of recommendation due to alleviating clinical symptoms,reducing adverse reactions occur,the relapse rate and improving the chances of conceiving infertility patients.

Objective To investigate the effects of oral calcium and active vitamin D on bone mineral density and balance function in senile osteoporosis patients.Methods A prospective casecontrol study was conducted on 216 senile osteoporosis patients who met the inclusion criteria.Patients were given oral calcium and active vitamin D for 12 months.The data values of BMD,balance function,falls incidence and bone pain were compared before versus 6 and 12 months after treatment.Results There were significant differences in BMD of lumbar vertebrae and femur neck,static balance function assessed by the single-leg stance timed tests with closed eyes,dynamic balance function assessed by the Star Excursion Balance Test(SEBT) in directions of east,southeast,south and southwest(all P＜0.05).The incidence of falls was decreased 12 months after treatment as compared with pre-treatment(5.6％ vs.12.5％).The bone pain had no significant difference before versus after treatment(P＞0.05).Conclusions Oral calcium and active vitamin D could increase BMD,improve the balance function and decrease the risk of falls in senile osteoporosis patients.However,it has no significant improvement on bone pain within a short period.

ObjectiveTo investigate and analyze the prevalence and risk factors of acute stress disorder (ASD) among survivors of road traffic accidents.MethodsA total of 206 participants ( 168 men,38 women) were administrated with the acute stress disorder scale (ASDS) 2 to 23 days after the traffic accidents.ResultsThere was a total of 61 (29.6％) possible ASD patients among all participants.Women had more serious ASD symptoms than men (male:36.70 ± 11.42; female:41.18 ± 12.58; P＜ 0.05 ),the married's ASD symptom severity was higher than the single's ( married:38.37 ± 11.94 ; single/widowed/divorced:30.86 ± 8.37 ; P ＜ 0.01 ),people with low degree of education had more severe ASD symptoms than the people who had high degree of education (P＜0.05) ; being elder( r=0.43,P＜0.01 ),having fear / helpless / horror experience of accident ( r=0.21,P＜ 0.01 ) were significantly correlated with the severity of ASD symptoms.According to linear regression analysis,age could positively predicted ASD symptoms ( β =0.29,P ＜ 0.01 ) too.ConclusionASD is a common mental problems among Chinese survivors of road traffic accident,and the main predictive factors of ASD include gender,age,marital status,the degree of education,whether there is fear / helpless / horror experience of accident.

Objective To explore the re-treatment approach for nonsmall-cell lung cancer(NSCLC)in clinic with therapeutic alliance of cryotherapy and interventional chemotherapy. Methods Sixty two patients with uncontrolled NSCLC after common radiotherapy and(or)chemotherapy were re-treated with therapeutic alliance of cryotherapy and interventional chemotherapy, and their serum tumor markers of NSE, CEA, CY21-1 and the value(I.S%,I.O.D%)of correlative protein in nucleolus region-Ag-NORs were tested before and after the re-treatment. Meanwhile, patients' one year survival and therapeutic effect to NSCLC were recorded during the follow up. Results After the re-treatment, distinguished decline of the serum tumor markers was observed(P

Objective To evaluate the effect of 5-aminolevulinic acid-based photodynamic therapy (ALA-PDT) on S.epidermidis biofilms.Methods S.epidermidis was cultured on cover glasses,with or without thepresence of ALA at 50 mmol/L for 16 hours followed by the exposure to different doses (100,200,300 J/cm2) of red light.S.epidermidis receiving neither pretreatment nor irradiation served as the negative control.Subsequently,confocal laser scanning microscopy (CLSM) was used to observe the structure and evaluate the biological activity of S.epidermidis biofilms; colony forming units (CFUs) of S.epidermidis were counted for the evaluation of killing effect of ALA-PDT; scanning electron microscopy (SEM) was used to observe the morphological structure of the biofilms.Results The number of colony forming units per milliliter (CFU/ml) was (210 ± 7.55) × 105,(91 ± 1.53) × 105,(16 ± 1.52) × 105 for S.epidermidis pretreated with ALA followed by irradiation with red light at 100,200 and 300 J/cm2 respectively,significantly different from untreated S.epidermidis ((388 ±8.89) × 105,all P ＜ 0.01) and S.epidermidis irradiated with red light only.Increased ratio of dead to live cells was observed in ALA-pretreated S.epidermidis irradiated with red light at 100,200 and 300 J/cm2compared with untreated S.epidermidis (1.254 ± 0.096,1.301 ± 0.160 and 3.410 ± 1.140 vs.0.358 ± 0.057,all P ＜ 0.01) and S.epidermidis irradiated with red light only.SEM showed that the biofilm structure was loose and obscure in S.epidermidis treated with ALA-PDT,and even disappeared with S.epidermidis distributed in single colonies when the dose of red light was 300 mJ/cm2.Conclusion ALA-PDT shows a potential bactericidal activity against S.epidermidis in biofilms,which can not only reduce biofilm vitality,but also damage biofilm structure.

Objective To explore the cost of healthcare-associated infection (HAI)management in a tertiary first-class hospital,provide data support for cost-effectiveness and cost-benefit analysis of HAI management,and provide scientific evidence for the rational allocation of hospital resources.Methods Micro-costing study was used to calcu-late the direct cost of the department of HAI management by collecting the quantity and unit price of each item. Results The total cost of HAI management in this hospital in 2013 were about ￥870 000,including human cost￥790 000,depreciated fixed assets ￥34 501 ,low-value consumption goods ￥3 800,publicity and training￥33 600,office consumables ￥5 208;average cost were ￥12.16 per person and ￥529.69 per bed.Conclusion Human cost is the main cost in HAI management in this hospital.

Objective To assess the application of the phage amplified biologically assay (PhaB) in the detection of the mycobacterium tuberculosis (TB) in sputum.Methods To determine the TB patients in the 53 suspected patients we detected 53 sputum samples by PhaB method,BACTEC MGIT960 rapid culture method,and conventional methods (direct auramine smear microscopy and concentrated auramine smear microscopy) individually.Results TB positive samples detected by PhaB,BACTEC MGIT960 rapid culture,direct auramine smear microscopy and concentrated auramine smear microscopy were 24,22,11 and 17 respectively.Thus the sensitivity,specificity,positive predictive value and negative predictive value were 86.4%,83.9%,79.2% and 89.7%,when the PhaB is combined with concentrated auramine smear microscopy,the sensitivity,specificity,positive predictive value and negative predictive value were increased up to 90.9%,83.9%,80.8% and 92.9%.Conclusion PhaB method is a simple and rapid method to detect the Mycobacterium Tuberculosis in sputum with a relatively higher sensitivity and specificity than the conventional methods.When combined with concentrated auramine smear microscopy,PhaB method is even more sensitive.Thus the PhaB method is accessible and applicable in the clinical use of the TB detection.