Objective To summarize the care measures of the cryosurgical treatment of primary liver cancer,and provide a theoretical basis for clinical treatment and care.Methods A retrospective analysis of 196 cases of primary liver cancer admitted in our hospital from August 2011 to October 2014 including clinical manifestation of adverse reactions and corresponding nursing care was made.Results Postoperative complications of the patients were rectified,and primary symptoms were also relieved.Conclusions Effective nurse care could reduce the incidence of postoperative complications of cryosurgical treatment for primary liver cancer patients,and improve patients' recovery.

Objective To study the influence of pine-seed oil upon body weight,blood lipid and lipid peroxidation of mice fed on high fat feed.Method The mice were fed by high fat feed(80% basal feed added 20% lard,HF) and HF added pine-seed oil(80% high fat feed added 20% pine-seed oil,SHF).At 45 d and 90 d,the body fat content,blood lipid level,and malonyldehyde(MDA),lipofusin(LP),catalase(CAT),superoxide dismutase(SOD) and glutathione peroxidase(GSH-Px) of brain and liver homogenate were measured respectively.Results Pine-seed oil could reduce the body weight serum triglyceride(TG),total cholesterol(TC),low density lipoprotein-cholesterol(LDL-C) content,but increase high density lipoprotein-cholesterol(HDL-C) of the mice fed by HF.It suggested that pine-seed oil had definite effect on regulating blood lipid.Compared with normal control(NC) group,the activities of CAT、T-SOD、GSH-Px of brain and liver in HF group were remarkably lower,but MDA and LP remarkably higher.It suggested that HF could promote lipid peroxidation in brain and liver of mice.Compared with HF group,the activites of CAT,T-SOD and GSH-Px of brain and liver in SHF group were remarkably higher,but MDA remarkably lower.Conclusion Pine-seed oil can reduce the body weight,blood lipid,and lipid peroxidation of mice fed by HF.

Objective To explore the clinical value of the serum cystatin C (Cys C) changes in different periods of the infantile pneumonia.Methods Ninety-two cases of infantile pneumonia (59 mild pneumonia patients and 33 severe pneumonia patients) from November 2012 to March 2013 were collected and 40 cases of healthy infant were enrolled for control group.The levels of serum Cys C,urea and creatinine in acute and recovery period were detected.Results In severe pneumonia patients,the average level of Cys C in acute period [(1.98±0.33) mg/L] is significantly higher than that in control group [(0.85 ±0.24) mg/L] (P ＜0.01),and there was no significant difference between recovery period [(1.12 ± 0.23) mg/L] and control group (P ＞ 0.05),and there was significant difference between acute and recovery period (P ＜0.05).In mild pneumonia patients,there were no significant differences in the level of Cys C between the acute period [(1.10 ±0.22) mg/L] and recovery period [(0.94 ±0.21) mg/L] as well as control group (P ＞ 0.05).The positive rate of Cys C in severe pneumonia patients (51.9％,14/27) was higher than that of urea and creatinine (3.7％,1/27) (P ＜0.01).Conclusion Severe pneumonia could result in renal dysfunction,which is reversible.Cys C can be the clinical reference for early diagnosis and the therapeutic effect in severe pneumonia patients with renal dysfunction.

Cytotoxic substances and ionizing radiation can easily induce DNA damage, and double strand breaks (DSBs) are the main form of DNA damage. DNA damage can activate intracellular DNA damage responses and further induce related biological effects, such as DNA damage repair and cell cycle arrest. Homologous recombination (HR) is the primary DSB repair mechanism in eukaryotes. Abnormal expression of Rad51C, which is a key factor in the HR pathway, may result in DNA repair disorder, genomic instability, and eventually lead to tumor formation. In recent studies, researchers considered Rad51C as a potential target for cancer treatment. We reviewed the research progress on Rad51C in DNA damage repair and radiotherapy.

Objetive To elucidate the mechanism of antibiotic resistance of Pseudomonas aeruginosa(P.a.) in an effort to provide a basis in clinical combined use of antibiotics against P.a.infection in clinical settings.Methods All P.a.strains were isolated by routine procedures and identified with VITEK-2 automatic bacterial identification console.The minimum inhibitory concentration(MIC) was detected using dilution method on agar plate following the instruction of CLSI.Results Ninety-two strains of P.a.were isolated from clinical infection specimens,most of them were obtained from respiratory tract(88.04%).Of the 92 strains,45(48.91%) were metallo-?-lactmases positive,and 25(27.17%) were AmpC positive.When treated with a combination of meropenem and imipenem,MIC≥1 was found in 17 strains(18.48%).The bacteriostatic rate of polymixin B and meropenem was 95.65% and 80.43%,respectively,that of amikaein,piperacillin/tazohaetam(PIT) and imipenem was 48.91%-71.74%,and that of cefoperazone/sulbactam(SUP) was 36.96%.The synergistic action of PIT,and SUP with amikacin was 60.87% and 58.70%,respectively,and that of PIT,SUP with minocycline were 44.57% and 43.48%,and of PIT,SUP with PLB were 28.26% and 7.61%,respectively.Conclusions P.a.strains in this study are mainly isolated from respiratory tract infection specimens.Multiple drug-resistant mechanisms are involved in the drug resistance of P.a.Enzyme inhibitors,such as PIT or SUP,with amikacin or polymixin B should be first selected for clinical treatment of P.a.caused infection.Meanwhile,antibiotics should be rationally administered in accordance with the seriousness of disease,and with the drug-resistant phenotype of the isolated strains.

Objective To study the clinical effect of cervical loop electrosurgical excision procedure (LEEP) in diagnosis and treatment of cervical diseases (including cervical intraepithelial neoplasia of different grade, cervical erosion of moderate and severe degree, cervical with human papillomavims (HPV) infection and cervi-cal polyp) . Method The data of 136 cases with cervical disease treated by LEEP were analyzed retrospectively. Re-sult The postoperative diagnosis were upgraded in 18 cases (13.2%) ,and downgraded in 38cases (27.9 %) . The preoperative diagnosis of 80 cases (58.8 %) were in accordance with the postoperative ones. The difference was sig-nificant(P<0.05). The mean operative time was 11minutes, the mean bleeding volume was 15 ml. Postoperative bleeding of LEEP occurred in 2.2 % (3/136) patients. Postoperative cervical conglutination of LEEP occurred in 1. 5 % (2/136) patients. The cure rate of CIN was 96 % after LEEP. The residual CIN occurred in 4 patient (4 %). Recurrence CIN occurred in 2 patient (2 %). At 12th months after LEEP,the negative rate of HPV detection was 86. 7% and the persistent positive rate was 13.3%. The HPV clearance rate was increased gradually after LEEP. Conclu-sion LEEP was safe and effective for the treatment of the cervical disease and was a kind of good method to clear HPV infection in cervical.

[ABSTRACT]AIM:Tostudytheeffectsofbaicalin(BC)onglialfibrillaryacidicprotein(GFAP)andnuclear factor-κB ( NF-κB) expression and neuronal apoptosis in juvenile rat hippocampus after status convulsion ( SC) .METH-ODS:One hundred and ninety five juvenile male Sprague-Dawley rats were randomly divided into 3 groups:normal saline pretreatment group (NS group), SC group and SC with BC pretreatment group (BC group).Each of these 3 groups would be subdivided into 5 subgroups sacrificed at 4 h, 12 h, 24 h, 48 h and 72 h after SC.The rat SC model was prepared by lithium-pilocarpine chemical method .The protein expression of GFAP and NF-κB was detected by the method of immuno-histochemistry .The mRNA expression of GFAP was detected by RT-PCR.The neuronal apoptosis was observed by TdT-mediated dUTP nick end labeling ( TUNEL ) .RESULTS: Compared with NS group , the GFAP positive cells was in-creased in SC group (P<0.05).Compared with SC group, the expression of GFAP was significantly reduced in BC group (P<0.05).Compared with NS group, the NF-κB positive cells was increased in SC group (P<0.05).Compared with SC group, the expression of NF-κB was significantly reduced in BC group .RT-PCR showed that the expression trend of GFAP mRNA was similar to that of the protein .Compared with NS group , the TUNEL positive cells in the hippocampus CA1 area in SC group increased significantly 12 h after SC (P<0.01), and reached a peak at 48 h.After the intervention with BC, the TUNEL positive cells decreased significantly between 12~48 h after SC (P<0.05 or P<0.01), but the number of TUNEL positive cells remained significantly greater than that in NS group (P<0.05).CONCLUSION: The expression of GFAP and NF-κB in the hippocampus increased after SC in rats .Baicalin decreases the expression of GFAP and NF-κB in hippocampus of rats with pilocarpine-induced seizures , and reduces the number of neuronal apoptosis , sug-gesting that baicalin may protect against the brain damage caused by status convulsion .

Objective To investigate couple's personality similarity effect on the marital quality,and to explore the vairance of such effect under different levels of spouse's depression. Methods The cross?sectional da?ta of 4644 couple from the national survey of mental health database in china( 2009) was used. Couples completed Locke ?Wallance marital adjustment test,The CES?D scale a self?report depression scale and Eysenck personality questionnaire short scale for Chinese version (EPQ?RSC). Results The personality similarities of couples(0.44 ±0.27) and their marital quality (wife (103.71±27.54),husband (106.15±26.94))had significant positive corre?lation ( r=0.25~0.27, P<0.01). Regression analysis showed the effect of personality similarity on the marital quality were varied according to the level of spouse depression( β=0.09?0.22, P<0.05) . Among participants whose husband or wife had higher level of depression,couple's personality similarity had stronger effect on his/her per?ceived marital quality. Conclusion Husband and wife personality similarity is related to marital quality signifi?cantly,and the spouse depression plays a significant moderating role in this relationship.

Objective To investigate the relationship between monocyte chemoattractant protein-1 (MCP-1) gene -2518G/A polymorphism and cerebral infarction(CI) of Han population in Chinese Hunan district.Methods The -2518G/A polymorphism in MCP-1 gene was detected by polymerase chain reaction-restriction fragment length polymorphism analysis (PCR-RFLP) and DNA sequencing in 162 patients with CI (CI group) and 150 normal controls (NC group). The serum level of MCP-1 in the two groups was determined by enzyme-linked immunosorbent assay (ELISA).Results In CI group, genotypic frequency of GG was 39.5%, GA was 37.0%, and AA was 23.5%. The allele frequency of G was 58.0% and A was 42.0%. In NC group, genotypic frequency of GG was 27.3%, GA was 39.3%, and AA was 33.4%. The allele frequency of G was 47.0% and A was 53.0%. The frequencies of MCP-1 -2518GG genetype and G allele in CI group were significantly higher than those in CI group (all P

Objective To study the condition of pheochromocytoma cells(PC12 cells)differentiation into neurons induced by nerve growth factor(NGF).Methods There were five groups :10,20,50,and 80 ?g?L-1 NGF groups and control group which contained 10% fetal calf serum.PC12 cells were exposed to NGF with different concentrations for 72 h and the lengh of neurite and max diameter of PC12 cells were observed at 24,48,and 72 h,respectively.The expression of MAP2 was detected by immunocytochemistry after PC12 cells were exposed to NGF for 72 h.Results The number of MAP2 positive cells was significantly increased in 20,50,and 80 ?g?L-1 groups compared with control group and the most significant concentration was 50 ?g?L-1(P