Objective To investigate the clinical significance and application value of the irregular antibody test before transfusion.Methods The irregular antibody test was conducted in 2 500 patients before blood transfusion.Results Through the analysis of irregular antibody examination of 2 500 patients with blood transfusion,15 cases appeared irregular antibodies in the serum,the positive rate was 0.60％.The positive rate of male irregular antibody was 0.12％ (3/2 500),the positive rate of female irregular antibody was 0.48％ (12/2 500).The positive rate of female irregular antibody was significantly higher than that of male irregular antibody (x2 =7.34,P ＜ 0.05) ; The irregular antibodies were mainly anti C,anti D,anti E and anti M type and non-specific antibody,the proportions were 13.3％,26.7％,20.0％,33.3％,6.7％.The incidence rate of blood transfusion hypersensitivity reactions in irregular antibody positive donor was 100.0％,that in irregular antibody negative patients was 0.0％,the difference between the two groups was statistically significant (x2 =9.28,P ＜ 0.05).Conclusion Irregular antibody test in patients before blood transfusion can effectively guide the patients to correct blood transfusion,and can effectively prevent and reduce blood transfusion hypersensitivity,improve the safety in the process of blood transfusion.

The article summarized recent studies on the composition and activity analysis,as well as the clinical application and mechanism of citrus aurantium and its prescriptions,in an attempt to provide theoretical evidence for the clinical application of citrus aurantium on improving gastrointestinal motility.

Cytokeratins can maintain the morphous and function of normal cells. Clinically, cytokera-tins are used to diagnose several tumors as tumor markers. Recently, numerous studies indicate that the abnor-mal expression, mutation in the site of phosphorylation and hyperphosphorylation have relationship with the mechnism of tumorigenesis. Reseach of the relationship is important for the diagnosis, therapy and prognosis of tumor.

Objective To study the reLationship between blood group antibody IgG titer of pregnant women and ABe hemolytic disease of newborn(HDN).Methods Blood group antibody IgG titem of 2700 pregnant women who is O and Rh positive with a non-O husband were detected.After bom,blood groups,Coomb's test,free antibody and antibody release test were done for those 280 babies who were in high risk of HDN.so that we could understand the relationship between the anlibody IgG level of pregnant women and ABO-HDN.Results The percent of anti-A (B)IgG titer＜64,64,128,256 and≥512 in the 2700 O type pregnant women was 62.4%,16.8%,12.6%,5.8%and 2.4%,respectively.There were 67 babies suffered from HDN in those 280 bahies who had high risk.which accounted for 23.9%.With the anti-A (B)IgG liter was 64,128,256 and≥512,the percent of HND positive was 10.2%,27.8%,45.7% and 50.0%,respectively (P＜0.01).Conclusion There is a positive relationship between blood group antibody IgG liter of pregnant women and ABO-HDN.It is necessary for the pregnant women to detect the level of anti-A(B)IgG.

New nano-fluorescent quantum dots (QD) have excellent optical properties, enabling them widely used in clinical cancer research. When combined with a variety of ligands, quantum dots have been successfully used for early detection of primary tumor, angiogenesis and metastasis, all of which have opened up a new frontier in early diagnosis of cancer.

Objective To investigate the feasibility of alanine aminotransferase (ALT)/aspartate aminotransferase (AST) ratio in predicting the degree of hepatic steatosis in chronic hepatitis C (CHC) patients.Methods A total of 231 CHC patients who visited The First People's Hospital of Nanyang from May 2012 to June 2016 were enrolled,among whom 105 (45.45％) had nonalcoholic fatty liver disease (NAFLD) and 126 (54.55％) did not have NAFLD.According to the ultrasound score,the NAFLD group was divided into mild-to-moderate (1-2 points) hepatic steatosis group (n =67) and severe (3 points) hepatic steatosis group (n =38).The two groups were compared in terms of demographic data and disease data including creatinine,fasting blood glucose,ALT,AST,ALT/AST ratio,γ-glutamyltransferase (GGT),uric acid,low-density lipoprotein,high-density lipoprotein (HDL),cholesterol (CHO),and triglyceride (TG) to screen out independent risk factors for NAFLD in CHC patients.The independent samples t-test was used for comparison of normally distributed continuous data between groups,and the Wilcoxon rank sum test was used for comparison of non-normally distributed continuous data between groups;the chi-square test was used for comparison of categorical data between groups;with severe NAFLD as the dependent variable,different factors were introduced into the logistic regression equation to screen out independent risk factors.Results Compared with the non-NAFLD group,the NAFLD group had significantly higher systolic pressure,diastolic pressure,fasting blood glucose,ALT/AST ratio,and levels of ALT,GGT,HDL,CHO,and TG,as well as a significantly higher proportion of patients with diabetes,hypertension,or metabolic syndrome (all P ＜ 0.05).Compared with the severe hepatic steatosis group,the mild-to-moderate hepatic steatosis group had significantly lower systolic pressure,diastolic pressure,fasting blood glucose,ALT/AST ratio,and levels of ALT,GGT,HDL,CHO,and TG,as well as a significantly lower proportion of patients with diabetes,hypertension,or metabolic syndrome (all P ＜ 0.05).The logistic regression analysis showed that metabohc syndrome,ALT,and ALT/AST ratio were independent risk factors for NAFLD (all P ＜0.05),and metabolic syndrome,ALT,ALT/AST ratio,and GGT were independent risk factors for severe NAFLD (all P ＜ 0.05).Conclusion ALT/AST ratio is an independent risk factor for NAFLD in CHC patients and has a certain value in evaluating the degree of NAFLD.

Objective To investigate the clinical effect of progesterone combined with psychological intervention on threatened abortion due to luteal insufficiency.Methods The control group was treated with progesterone alone, and the study group was given psychological intervention on the basis of oral progesterone.Two groups of corpus luteum dysfunction in patients with threatened abortion clinical effects and bad mood improvement effect.Results The study group of corpus luteum dysfunction in patients with threatened abortion success rate (93.75%) was significantly higher than the control group (77.08%), data comparison(P<0.05);study group of SDS, SAS scale score improved better than the control group, comparison of the data(P<0.05).Conclusion Application of progesterone combined with mental intervention in the treatment of corpus luteum dysfunction aura the abortion effect, help to protect patients' quality of life, physical and mental health.

Establishing a teaching quality control system and strengthening the supervision to the teaching quality is an important means and guarantee of the constant improvement and perfection of the quality of teaching at affiliated hospitals

Objective To summarize the care measures of the cryosurgical treatment of primary liver cancer,and provide a theoretical basis for clinical treatment and care.Methods A retrospective analysis of 196 cases of primary liver cancer admitted in our hospital from August 2011 to October 2014 including clinical manifestation of adverse reactions and corresponding nursing care was made.Results Postoperative complications of the patients were rectified,and primary symptoms were also relieved.Conclusions Effective nurse care could reduce the incidence of postoperative complications of cryosurgical treatment for primary liver cancer patients,and improve patients' recovery.

Objective To explore the effect of air impact method clearing out the subballonet secretion on the prevention of ventilator-associated pneumonia(VAP) for ventilation patients.Methods 108 cases from ICU who received mechanical ventilation were selected.Patients were divided into the observation group and control group according to odd and even numbers,54 cases in each group.The control group was received conventional airway management,and the observation group received air impact method on the basis of conventional management Results of blood gas analysis,vital signs,occurrence time of VAP,total incidence of VAP,mortality,OB,positive results during mechanical ventilation were observed.Results Occurrence time of VAP for observation group and control group respectively were (14.1 ± 5.2) d and (8.9 ± 3.8) d.VAP incidence in 1 week for observation group and control group respectively were 7.4% (4/54) and 27.8% (15/54).Test positive result of secretion OB for observation group and control group respectively were 16.7% (9/54) and 3.7% (2/54).Total Incidence of VAP for observation group and control group respectively were 33.3% and 37.0%.Differences of occurrence time of VAP,VAP incidence in 1 week and test positive result of secretion OB for two groups were statistically significant (t =5.933,x2 =7.728,4.960,all P ＜ 0.05).Differences of results of blood gas analysis,vital signs,total incidence of VAP,mortality for two groups had not statistically significant.Conclusion Air impact method cleating out the subballonet secretion is simple and safe.Occurrence time of VAP was delayed.Incidence of VAP in one week decreases.,which creates opportunity for rescuing patients.