The paper reports the effect of drugs on the Fc receptor(FcR)and C3b receptor (C3bR)of mouse peritoneal macrophages which was investigaed using in vitro EA-rosette and Yc-rosette techniques,for a further study on mechanism of anti-infection effect of Sheng Ji Ointment.The result of experment has showed that EA-rosette and Yc-rosetteforming rates of Sheng Ji Ointmen activated peritoneal macrophages were higher thanthe controls (p

Objective:To study the association between mutations in the first and second exons of(Succtnate dehydrogenaes complex,subunit D(SDHD)gene and sporadic pheochromocytoma in Chongqing Methods:Polymerase chain reaction combining with single strand conformation polymorphism(PCR-SSCP)was selected to analysis the mutations in the first and second exons of SDHD gene in 32 cases of sporadic pheochromocytoma who were diagnosed by pathology(including 21 cases of pheochromocytoma and 11 cases of extra-adrenal paraganglioma)and 80 cases of the healthy controls.Results:No mutation was found in the first and second exons of SDHD gene in the 32 patients.Conclusion:In our study,we had not found any mutation in the first and second exons of SDHD gene in sporadic pheochromo-cytoma in Chongqing.

Hypertrophic cardiomyopathy is regarded as an inherited cardiac disorder caused by autosomal dominant mutations.It has the remarkable heterogeneity of hereditary capacity,clinical phenotype,clinical course and prognosis,thus the diagnosis and treatment of this disease is challenging.Assistant examinations,such as Doppler tissue imaging,quantitative tissue velocity imaging,tissue strain imaging,cardiac magnetic resonance and late gadolinium enhancement,are important to early diagnose,guide management and judge prognosis for hypertrophic cardiomyopathy.This paper reviews the progresses of assistant examinations in hypertrophic cardiomyopathy.

BACKGROUND: Induced pluripotent stem cells are obtained from somatic cells by reprogramming method. The safety of induced pluripotent stem cells has attracted much attention because of their huge and potential value in clinical application. OBJECTIVE: To review the current studies addressing the safety and clinical application of induced pluripotent stem cells. METHODS: The PubMed database between 2006 and 2012 was retrieved by the first author to search the correlative documents concerning the safety and clinical application of induced pluripotent stem cells. Total y 203 papers were primarily gotten. Final y, 47 papers were included. RESULTS AND CONCLUSION: At present, the main methods to enhance the safety of induced pluripotent stem cells include avoiding usage of c-Myc gene, another mediate way replacing the retrovirus, direct leading of reprogramming factor protein, safer donor cells, micromolecule compound and other in-transgenosis ways. Induced pluripotent stem cells have extensive clinic treatment prospects, and can be used for the build of disease-specific induced pluripotent stem cells line.

BACKGROUND: Oyster shell has been recently reported as a new root canal filling material; therefore, it needs primary biological evaluation, i.e., cytotoxicity test and genotoxicity test, before clinical application. OBJECTIVE: To evaluate cytotoxicity and genotoxicity of oyster shell powder. DESIGN, TIME AND SETTING: A contrast observational study was performed at the Materials Laboratory, School of Stomatology of Peking University from April to July 2008. MATERIALS: Oyster shell powder was provided by Institute of Oceanology, Chinese Academy of Sciences; L929 cell strain and Salmonella typhimurium TA97, TA98, TA100, and TA102 were provided by the National Institute for the Control of Pharmaceutical and Biological Products. METHODS: Oyster shell diffusion was prepared based on the times of 0.5, 1, and 2. According to the standards of biological evaluation of dental materials, the cytotoxity and genotoxicity of oyster shell were evaluated with molecule filter test and Ames test. MAIN OUTCOME MEASURES: Fading area of the film induced by succinic dehydrogenase and amount of spontaneous revertants. RESULTS: After 2 hours, the results indicated that fading area of the film in the experimental group was the same as negative control, i.e., fading was not found in the film, and cytotoxicity was grade 0. After 24 hours, diameter of non-stained film was 1.0-2.0 mm, fading area was 1-3 mm2, and cytotoxicity was grade 1, suggesting that the material had light cytotoxity that was still qualified. The spontaneous revertant test indicated that the mutagenicity of experimental groups was no more than 2 times of the control groups, suggesting there was no dose-dependence. CONCLUSION: Oyster shell powder has no cytotoxicity and genotoxicity on the organism.

As a special branches of capillary electrophoresis(CE), nonaqueous capillary electrophoresis (NACE) was variedly applied in the recent years. A brief summary on solvent properties and molecular interactions in nonaqueous solutions are introduced. Discussing of the approaches employed to enhance and tune selectivity in NACE. Special detection techniques for NACE are presented. Furthermore, a list of relevant applications in biological sample and its metabolin and also in pharmaceutical since 2000 is included, and also take perspective for NACE.

Serum metalloproteinase matrix (M MP )-9, tissue inhibitor of metalloproteinase (TIMP)-1 and type Ⅳ collagen level s were measured in patients with diabetic nephropathy (DN). The severity of DN w as determined by urinary albumin excretion rate. The results show that signific ant increases of serum TIMP-1 and type Ⅳ collagen depend on severity of DN, wh ile MMP-9 tends to decline. TIMP-1 and MMP-9 seem to participate in the patha genesis of DN.

Objective To observe the central retinal artery (CRA) hemodynamics of diabetic patients befroe and after treatment with fosinopril.Methods 19 cases (38 eyes) were treated with fosinopril,10 mg/d.Peak systolic velocity (Vs),mean velocity (Vm),endodiastole velocity (Vd),pulse index (PI) and resistance index (RI) of CRA were measured before and one month after treatment using color doppler flow imaging and compared with that of controls.Results Before treatment with fosinopril the CRA Vs,Vm and Vd of the patents were lower but PI and RI were higher than that of controls.After treatment the CRA Vs,Vm and Vd of the patients were increased but PI and RI were decreased (P

Objective To evaluate color Doppler sonography in the diagnosis of local recurrence in patients undergoing Miles procedure for rectal carcinoma.[WT5”HZ] Methods Transperineal and transsacral color Doppler blood flow sonography was performed on 51 patients presenting presacral masses after Miles procedure for rectal carcinoma. Blood flow richness and the blood flow dynamics indexes e.g. peak systolic velocity (PSV), diastolic velocity (EDV),resistive index (RI), and pulsatility indexs (PI) were recorded and analyzed. Results Blood flow signals in the recurrent masses were mainly Ⅱ-Ⅲ amounting to 81 82% (27/33), and that of benign masses were mainly 0-Ⅰ,Ⅱ-Ⅲ amounting to 27 78% (5/18). Both were significantly different (? 2=14 55, P

ObjectiveTo investigate the correlation factors of macular pucker after vitrectomy, and compare the surgical effect between secondary and idiopathic epiretinal macular membrane.MethodsA study of 26 consecutive patients (26 eyes), in which 18 were diagnosed as secondary epiretinal macular membranes and 8 as idiopathic epiretinal macular membranes, underwent par plana vitrectomy, vitreous cortex separation, and peeling off of the epiretinal macular membrane. The follow-up period was within 3~12 months. Preoperative and postoperative examination included visual acuity, fundus photography, and optical coherence tomography in some patients.ResultsAmong the 18 patients with secondary epiretinal macular membranes, 9 (50.0%) were related to vitreous surgery. There was statistical difference between cryoretinopexy to giant retinal tear and endolaser (?2=12.24,P