Objective To investigate the effect of Nifekalant on action potential of atrium and pulmonary vein and the possible clinical efficacy of Nifekalant against atrial tachyarrhythmias.Methods left and right atrium and pulmonary vein removed from 15 white rabbits were made into tissue preparations(0.5 cm?1.5 cm).Action potential was induced by electric stimulation with twofold capture threshold and recorded by standard glass microelectrode technique.Effective refractory periods of left and right atrial and pulmonary vein tissues were measured with burst and programmed stimulation.The Action potentials and effective refractory periods of various tissues were compared between before and after irrigation of Tyrodes fluid containing Nifekalant of 2.13 mg/L.Results After irrigation of Tyrodes fluid containing Nifekalant,APD90 was prolonged[(51?16)ms vs(78?33)ms,P

As a special branches of capillary electrophoresis(CE), nonaqueous capillary electrophoresis (NACE) was variedly applied in the recent years. A brief summary on solvent properties and molecular interactions in nonaqueous solutions are introduced. Discussing of the approaches employed to enhance and tune selectivity in NACE. Special detection techniques for NACE are presented. Furthermore, a list of relevant applications in biological sample and its metabolin and also in pharmaceutical since 2000 is included, and also take perspective for NACE.

AIM:To establish a kind of simple and efficient method for cell-free fetal DNA ( cff-DNA) enrich-ment and to investigate its range of applications and the advantages and disadvantages.METHODS:(1) The single nucleo-tide polymorphisms( SNPs) , which linked to paternalβ-thalassemia mutations, were screened.We analyzed the contact be-tween the SNPs inβ-thalassemia gene ( HBB gene) and haploid type by the Haploview software, and then selected these close SNPs which have higher heterozygosity with the HBB gene.(2) We selected 4 cases of different β-thalassemia muta-tions with their husband, and then we used TT-FAST-COLD-PCR to enrich the IVS-II-654 mutations in maternal plasma.If the IVS-II-654 mutation was not detected, we detected the SNP which linked to the IVS-II-654 mutation.Similarly, we used TT-FULL-COLD-PCR to enrich the CD41-42 mutations in the maternal plasma.At the same time, we used the conventional PCR to enrich CD41-42 mutation and IVS-II-654 mutation in the maternal plasma.RESULTS:(1) Nine cases of the SNP ( rs7480526) linked to the mutation at IVS-II-654 in HBB gene, and 11 cases of the SNP ( rs10768683) linked to the muta-tion at CD41-42 in HBB gene were detected.( 2 ) We detected 1 case who inherited the paternal β-thalassemia mutation (IVS-II-654).We did not directly detect patermal IVS-II-654 mutation in maternal plasma, but detected the SNP linked to the IVS-II-654 mutation in the other case and had 100%detection, and 2 cases inherited the paternal β-thalassemia muta-tions (CD41-42) in the maternal plasma by TT-FULL-COLD-PCR and had 100%detection.However, we detected nothing by conventional PCR.CONCLUSION:TT-COLD-PCR is applicable to enrich cell-free fetal DNA in maternal plasma and is a method in the field of noninvasive prenatal diagnosis.

Objective To study the effect of acupoint sticking combined with acupuncture after thoracoscopic surgery on the improvement of pain.Methods A total of 160 patients undergoing thoracoscopic surgery in our hospital were divided into observation group and control group,with 80 cases in each group.The patients in the control group were only used PCA self controlled analgesia to relieve pain.The patients in the observation group were used acupoint sticking at 4 h after surgery,and the acupuncture treatment was implemented at 4,8,24 and 48 h after operation on the basis of control group.The patient's visual analog scale (VAS) score and the pain questionnaire (SF-MPQ)score were compared,and the treatment efficiency after 48 h was statistically analyzed.Catecholamine (DA),thyroid hormone (NA),and adrenaline (Ad) in peripheral blood at 4,48 h of two groups were compared.Results The VAS scores and SF-MPQ scores of the two groups were all decreased,and the observation group decreased more significantly than that of the control group (P ＜ 0.05),and the observation group had better treatment efficacy than the control group (P ＜ 0.05).There were no significant differences in DA,NA and Ad between the two groups (P ＞ 0.05).Conclusion Acupoint sticking combined with acupuncture has significant effect on relieving pain of patients after thoracoscopy.

Objective To study the effect of acupoint sticking combined with acupuncture after thoracoscopic surgery on the improvement of pain.Methods A total of 160 patients undergoing thoracoscopic surgery in our hospital were divided into observation group and control group,with 80 cases in each group.The patients in the control group were only used PCA self controlled analgesia to relieve pain.The patients in the observation group were used acupoint sticking at 4 h after surgery,and the acupuncture treatment was implemented at 4,8,24 and 48 h after operation on the basis of control group.The patient's visual analog scale (VAS) score and the pain questionnaire (SF-MPQ)score were compared,and the treatment efficiency after 48 h was statistically analyzed.Catecholamine (DA),thyroid hormone (NA),and adrenaline (Ad) in peripheral blood at 4,48 h of two groups were compared.Results The VAS scores and SF-MPQ scores of the two groups were all decreased,and the observation group decreased more significantly than that of the control group (P ＜ 0.05),and the observation group had better treatment efficacy than the control group (P ＜ 0.05).There were no significant differences in DA,NA and Ad between the two groups (P ＞ 0.05).Conclusion Acupoint sticking combined with acupuncture has significant effect on relieving pain of patients after thoracoscopy.

OBJECTIVETo investigate association between the lysyl oxidase-like 1 (LOXL1) gene single nucleotide polymorphism (SNP) and primary open-angle glaucoma (POAG) in Sichuan population.

METHODSIn this study,416 subjects with primary open-angle glaucoma and 997 normal controls were recruited.Three reported LOXL1 tag SNPs (rs1048661,rs3825942 and rs2165241) were genotyped by SNaPshot method.

RESULTSThe study showed that the genotypes of LOXL1 rs1048661,rs3825942 and rs2165241 between POAG and control groups were not statistically significant (OR=1.085, 95%CI 0.92-1.28, P=0.578 for rs1048661; OR=1.059, 95%CI 0.82-1.37, P=0.846 for rs3825942; OR=1.006, 95%CI 0.77-1.32, P=0.966 for rs2165241, respectively). There were no significant difference in allele frequency distribution of LOXL1 rs1048661、rs3825942 and rs2165241 between POAG and normal controls (P=0.322, P=0.660, P=0.965).

CONCLUSIONThe results from the present study do not indicate the association of LOXL1 SNPs (rs1048661, rs3825942 and rs2165241) with POAG in Sichuan population.

Adult ; Aged ; Aged, 80 and over ; Amino Acid Oxidoreductases ; genetics ; Asian Continental Ancestry Group ; genetics ; Glaucoma, Open-Angle ; genetics ; Humans ; Middle Aged ; Polymorphism, Single Nucleotide

Objective:To observe the diagnostic value of six classification intelligent auxiliary diagnosis lightweight model for common fundus diseases based on fundus color photography.Methods:A applied research. A dataset of 2 400 color fundus images from Nanjing Medical University Eye Hospital and Zhejiang Mathematical Medical Society Smart Eye Database was collected, which was desensitized and labeled by a fundus specialist. Of these, 400 each were for diabetic retinopathy, glaucoma, retinal vein occlusion, high myopia, age-related macular degeneration, and normal fundus. The parameters obtained from the classical classification models VGGNet16, ResNet50, DenseNet121 and lightweight classification models MobileNet3, ShuffleNet2, GhostNet trained on the ImageNet dataset were migrated to the six-classified common fundus disease intelligent aid diagnostic model using a migration learning approach during training as initialization parameters for training to obtain the latest model. 1 315 color fundus images of clinical patients were used as the test set. Evaluation metrics included sensitivity, specificity, accuracy, F1-Score and agreement of diagnostic tests (Kappa value); comparison of subject working characteristic curves as well as area under the curve values for different models.Result:Compared with the classical classification model, the storage size and number of parameters of the three lightweight classification models were significantly reduced, with ShuffleNetV2 having an average recognition time per sheet 438.08 ms faster than the classical classification model VGGNet16. All 3 lightweight classification models had Accuracy > 80.0%; Kappa values > 70.0% with significant agreement; sensitivity, specificity, and F1-Score for the diagnosis of normal fundus images were ≥ 98.0%; Macro-F1 was 78.2%, 79.4%, and 81.5%, respectively.Conclusion:The intelligent assisted diagnosis of common fundus diseases based on fundus color photography is a lightweight model with high recognition accuracy and speed; the storage size and number of parameters are significantly reduced compared with the classical classification model.

This paper discussed how to set the limit of impurities in the quality standard when the impurities in small-molecular innovative drugs were metabolites.Firstly,through the analysis and interpretation of each relevant guideline,in combination with the review cases of FDA and EMA,found out the conditions for qualification of im-purity,and then establ ish the acceptable limit of impurities based on the test results of multiple batches,the incre-ments of production process and storage process.

Severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)is the main pathogen that causes COVID-19,which is fast-mutating and highly transmissible.The infection has led to a global epidemic.As the main preventive and control measure,vaccination plays a critical role in fighting a-gainst COVID-19.Although a large number of epitope-based and mucosal vaccines have been stud-ied,few peptide epitope vaccines targeting the mucosa and their functional evaluation have been re-ported.In this study,we used SARS-CoV-2 structural protein M peptide epitope predicted by the IEDB database as an antigenic target to design the MS-3S gene containing 3 050 and 1 229 signal peptides and DCpep optimized for insertion into MS2 phage coat proteins.The expression plasmid pSIP:MS-3S was constructed by cloning the PCR fragments seamlessly and was transformed into Lactiplantibacillus plantarum 18 to obtain the recombinant bacterium LP18:MS-3S.Expression conditions such as induction time,inducer concentration,rotational speed and initial pH were opti-mized.The intranasal immunization experiments were performed to examine the vaccine efficacy.The results showed that the 916 bp-long target gene MS-3S modified and optimized was amplified and used to successfully construct the recombinant bacterial strain LP18:MS-3S.The optimal con-ditions for recombinant protein expression were obtained and verified by Western blot,flow cy-tometry,immunofluorescence and other detection methods.The optimal expression conditions were determined as follows:induction time was 4 h with 100 pg/L of SppIP as the optimal induction concentration.Antibody-specific for the epitope was verified by ELISA experiments in serum,alve-olar lavage fluid and fecal dilutions of mice.In summary,a recombinant bacterial strain expressing the epitope antigen of the SARS-CoV-2 M protein peptide was constructed.The obtained protein can induce the body to produce humoral and mucosal immunity,which lays the foundation for the development of a vaccine candidate for the mucosal immunity of COVID-19.

Lung cancer is one of the most common malignancies with the highest incidence rate and mortality rate worldwide, and non-small cell lung cancer (NSCLC) accounts for about 85%. Only 5% NSCLC patients are anaplastic lymphoma kinase (ALK) rearrangement positive NSCLC, but the prognosis of these patients is poor, and treatment is urgent. Ensartinib (X-396), a next-generation ALK tyrosine kinase inhibitor (ALK-TKI), has shown greater potency on inhibiting ALK activity and controlling brain metastases than crizotinib, which is indicated for the treatment of crizotinib-resistant, ALK-positive NSCLC patients. Several phase I to III clinical trials included both healthy volunteers and NSCLC patients have been conducted both in China and abroad. In this review, we briefly summarized the results of these trials, and preliminary efficacy, safety, pharmacology and pharmacokinetics/pharmacodynamics of ensartinib were discussed.