Objective:To establish a method for the determination of 4-hydrazinobenzenesulfonamide hydrochloride in celecoxib. Methods:HPLC was used and the chromatographic column was a Hypercarb series C18 column (150 mm × 4. 6 mm, 3 μm) with 100% porous graphite as the filler. The mobile phase consisted of 10 mmol·L-1 ammonium acetate aqueous solution-methanol (50 :50) and methanol. The flow rate was 0. 8 ml·min-1 and the detection wavelength was 220 nm. The column temperature was 35 ℃and the injection volume was 20 μl. Results:The linear range was 99. 897-599. 382 ng·ml-1(r=0. 9982) for 4-hydrazinobenzene-sulfonamide hydrochloride. The average recovery was 97. 4%(RSD=3. 0%,n=9). Conclusion:The method is simple and accurate, and can be used for the determination of 4-hydrazinobenzenesulfonamide hydrochloride in celecoxib.

FoxO as an important transcription factors,can be deacetylated by SIRT1 (a sort of deacetylases),SIRT1-FoxO-au-tophagy pathway is likely to play a vital role in some diseases,e. g.diabetes,aging,obesity,cancer,cardiovascular diseases and muscle atrophy,etcetera.This paper aims to analy transcription-al regulation mechanism of FoxO,and reviews the progress of SIRT1-FoxO-autophagy pathway.

ObjectiveTo determine the bispectral index and dose of propofol at which 50 % of patients fall into unconsciousness during induction (BIS50 and EDs0 ). MethodsThirty unpremedicated ASA Ⅰ-Ⅱ patients scheduled for elective surgery were included in this study. The patients did not take any sedatives or hypnotics and had no psychiatric disease. Loss of consciousness and changes in BIS during induction with propofol were studied by sequential experimental method. The loss of responses to verbal command and eyelash stimulation was used as an induction of unconsciousness. The data obtained were analyzed by linear regression and calculated to determine BIS50 and ED50 using weighted averaging method. ResultsThe BIS50 and ED50 for propofol were 79.17 and 1.0192 ng/kg with the 95％ confidence intervals of 72.08-88.55 and 0.94-1.148 mg/kg respectively. Conclusions The sequential experimental method for determination of ED50 is simple, efficient and reliable and also suitable for determination of BIS50.

Objective To investigate the students' perception and effectiveness of the flipped class model in bilingual education in special population nutrition class.Method Two classes of Shanghai University of Traditional Chinese Medicine were exposed to either the flipped class model or the traditional class model.In the flipped class model,the students were provided a wealth of teaching resources such as short videos before class.In addition,a series of pedagogical approaches were used to develop the students' cognitive ability.The questionnaire survey and class quiz were employed to evaluate the students' perception and effectiveness of the flipped class model.Results The students in the experimental group have significant higher scores in professional vocabulary section [(23.10 ± 0.71) vs.(21.10 ± 0.67)],listening and speaking section [(21.20 ± 0.78) vs.(19.00 ± 0.74)],essay questions section [(37.80 ± 0.96) vs.(34.40 ± 1.15)],and total test scores [(82.20 ± 1.50) vs.(74.50 ± 1.52)] than did the students in the control group.In addition,the flipped class model ignites the students' learning interest,reduces the learning anxiety,and develops their learning autonomy.Conclusion The flipped class model is a highly effective means that promotes the student centered active learning.This study provides reference for implementation of the flipped class model in Chinese medical bilingual education.

ObjectiveTo study the correlations among age,sex,eye and mERG results.Methods60 normal eyes of 46 volunteers were divided into 6 groups at different ages.RETIscan 3.12 system of Rodenstock was used to carry out mERG examination.ResultsVariation of mERG parameters among normal eyes was significant.Age,sex and eyes have little influence on mERG results,except latency A was longer in male than in female.The size of pupil and fixation were found to influence the results directly.ConclusionsCare should be exercised in explaining the results.Disease history and other examinations shoud be taken into consideration.Attention should be given to the changes in wave-form and vision mountain in addition to amplitudes and latencies.

Objective To discuss the relationship between bone mineral salt metabolism index change and bone density in different stage of chronic kidney disease (CKD).Methods Three hundred and forty-five non-dialysis patients with CKD were enrolled in this study.According to the serum creatinine (SCr),glomerular filtration rate (GFR),they were divided into stage 1-5.CKD 1 had 75 cases,CKD 2 had 65 cases,CKD 3 had 65 cases,CKD 4 had 65 casès,CKD 5 had 75 cases.The level of blood calcium (Ca),phosphorus (P) and SCr were detected by ion selective electrode method.The level of urea nitrogen (BUN) was detected by picric acid method.The level of intact parathyroid hormone (iPTH) and 1,25 (OH)2VitD3 were detected by chemiluminescence.Results With the decline of renal function,the level of iPTH and blood P increased gradually,especially in stage CKD 4 and CKD 5,and compared with stage CKD 1,there were significantly differences [(289.6578 + 187.7945),(482.0667 + 232.2065) μ g/L vs.(77.8431 + 52.9760) μ g/L and (1.6938 + 0.1960),(2.6034 ± 0.5986) mmol/L vs.(1.3833 ± 0.4068) mmol/L,P ＜ 0.05].The level of blood Ca and 1,25 (OH)2VitD3 decreased gradually,especially in stage CKD 4 and CKD 5,and compared with stage CKD 1,there were significantly differences [(1.9185 +0.4633),(1.8293 ± 0.3075) mmol/L vs.(2.0893 + 0.2245) mmoi/L and (10.0892 + 0.2638),(7.6058 +0.4356) ng/L vs.(18.0036 + 0.3218) ng/L,P ＜ 0.05].With the decline of renal function,the proportion of osteoporosis also gradually increased,especially in stage CKDS,and compared with stage CKD 1 and CKD 2,there was significantly difference [60.00％ (45/75) vs.20.00％ (15/75),23.08％ (15/65),P ＜ 0.05].Conclusion With the decline of renal function,the level of blood P and iPTH increase,and blood Ca,1,25(OH)2VitD3,bone density decrease,especially in stage CKD 4 and CKD 5 patients.

The research of bone tissue engineering provides new thought and method to repair mass bone defect. Neovascularization plays a significant role in bone repair. This article reviews the advancements of the growth factors, seed cells and scaffolds in vascularization of tissue engineering bone, then raise the problems to solve and the prospect of future research.

Objective: To study the inhibition and mechanism of tea polyphenols (TP) on apoptosis induced by oxidized low density lipoprotein (ox-LDL) in human umbilical vein endothelial cells (HUVEC). Method: HUVEC cells were administered with TP (25 ?g/ml), ox-LDL (200 ?g/ml)+TP (25 ?g/ml), ox-LDL (200 ?g/ml) respectively, cells with equal volume of solvent as control. Cell viability was determined by MTT assay, apoptosis by acridine orange fluorescence dyeing, and expressions of Bcl-2、Bax and caspase-3 were analyzed by Western blotting. Results: Ox-LDL could inhibit HUVEC cell proliferation. After treated with both TP and ox-LDL , the cell proliferation increased obviously and showed significant difference from the cells treated with ox-LDL only (P

Objective To evaluate the variability of four parameters of multi-focal electroretinogram (mERG) a-wave amplitude, b-wave amplitude, a-wave latent period, b-wave latent period. Methods Sixty normal eyes of 46 volunteers were divided into 3 groups of different ages. RETIscan 3 12 system was used to carry out mERG examination. The stimulus matrix of 61 hexagonal elements spanning the central 24?of the visual field. These hexons were scaled with eccentricity and divided into 5 rings. First-order kernel was selected. Results The variability of four parameters of mERG was great. The variability of b-wave latent period was the smallest, its coefficient of variatian was 4.52%～15.62%;that of a-wave latent period held the second place:10.29%~48.67%;that of b-wave amplitude was greater:25.92%~76.11%;that of a-wave amplitude was the greatest:43.82%~88.23%. The results of three groups showed that b-wave amplitude of ring 1 had the smallest variability. Conclusions The variability of latent period is smaller than that of amplitude; the variability of b-wave was smaller than that of a-wave. The longer the centrifugal distance, the lower the amplitude density of a-wave and b-wave. Physiological and anatomical factors might be important for the variability of parameters of multi-focal electroretinogram.

With the rapid development of medical sciences in recent years,tissue engineering technology has made great achievements,while the choosing of seed cells has been a major focus of the field.Adipose-derived cells have the advantages of rapid expansion,good stability,no immune rejection and potential of multiplex differentiation.They can differentiate into cells originated from different germ layers such as:adipocytes,osteoblasts,chondrocytes,endothelial cells,myocytes,neuronal cells and so on.Besides,adipose tissue can be harvested easily and substantially with small trauma to human body.So ADSCs are expected to be an ideal choice of seed cells in tissue engineering.This article introduces the research progress of ADSCs in osteochondral tissue engineering.