Systemic insulin resistance has been well known. Recently, an important question has been raised regarding whether islet cells are resistant to insulin (insulin resistance of ?, ? and ? cells) due to the discovery of insulin receptor and its downstream insulin signaling pathways in islet cells. As a new study field of insulin resistance, the elucidation to this issue is crucial for the new insight into the mechanisms of insulin resistance per se and type 2 diabetes.

Non-receptor tyrosine kinase c-Abl has been implicated in the regulation of the cell cycle,adhesion, apoptosis and DNA damage responses.Its excessive activation is related with the development of chronic myeloid leukemia, and the relationship between c-Abl and leukemia is in depth and detailed research.In contrast to leukemia, the research between c-Abl and solid tumors is just at the early stage.This paper briefly reviews research progress of the c-Abl in solid tumors.

miRNA is a kind of small,endogenous noncoding RNA which regulates gene expression in post-transcription level and is highly conserved and tissue-specificity,miRNA generally participates the regulation of many complicated vital process,such as cell growth,development and also plays an important role in the pathogenesis of many kinds of tumors,miR-34b is a newly discovered miRNA,which is regulated by p53,promoter methylation and so on.miR-34b can regulate cellular proliferation and apoptosis,and also involves in the generation and regulation of tumor,which suggests wide application prospect in therapy and prognosis of tumor.This review will focus on the progress of miR-34b and its relationship with tumor.

Recent evidences suggest that inflammatory factors play improtant roles in the pathogenesis and development of type 2 diabetes. The interaction of inflammatory factors with adipokines, oxidative stress and immune system causes insulin resistance and impairment of ? cell structure and function, and results in type 2 diabetes. Inflammation hypothesis of diabetes will provide a new research field in the pathogenesis of diabetes and in the development of new preventive and therapeutic drugs for diabetes.

Spironolactone has been widely used in clinical treatment of PA,but its adverse effects are prominent when at large dose.So it is very important for clinicians to choose proper medications and doses for PA.With widespread use of selective aldosterone-receptor antagonists,non-aldosterone antagonists diuretic,calcium antagonists,angiotensin-converting enzyme inhibitors and angiotensin-receptor blocker in treatment of PA,clilicians now have more choice.This review introduces the progress in medications of PA in recent years.

ObjectiveTo assess the value of intraoperative cholangiography(IOC) for detecting bile duct injury during laparoscopic cholecystectomy.Methods31 cases of bile duct injuries were reviewed regarding the site of injury,mechanism,time of diagnosis,and the place of IOC was evaluated.ResultsBile duct injuries resulted from misidentification in 19 cases.In 12 of these 19 cases,the diagnosis was made by IOC.Primary laparoscopic repair or open repair and T-tube drainage solved the problem without postoperative complications.However,in 3 of the above 19 cases the result of IOC was misinterpreted and in 4 of the above 19 cases IOC was not performed.Two of the 7 patients had aberrant bile duct injuries, in all of them the diagnoses were delayed.There were two cases in which bile duct injury developed after an IOC.ConclusionsCorrect interpretation of IOC enables detecting bile duct injury caused by intraoperatively misidentification and consequent morbidity.

AIM　To investigate the therapeutic effect of boanmycin (BAM, bleomycin A6) on colon carcinoma 26 and its hepatic metastasis in mice. METHODS　A series of models including subcutaneous transplantation, orthotopic transplantation in cecum subserosa, intra-hepatic transplantation of tumor, and intra-splenic transplantation of tumor accompanied with hepatic metastases were employed. In addition, LEICA Q 500IW image analysis system was used to determine the area of metastatic lesions in the liver in histopathological sections. RESULTS　BAM at 5 mg*kg-1 and 2.5 mg*kg-1 inhibited the growth of subcutaneous tumors by 78.7% and 61.9%, and the orthotopic tumors by 99.4% and 90.0%; and the intra-hepatic tumors by 86.9% and 75.7%, respectively. Determined by the numbers of metastatic nodules, BAM at 10 mg*kg-1 and 5 mg*kg-1 inhibited hepatic metastases from intra-splenic transplantation by 97.6% and 56.8%; and evaluated by image analysis of metastatic lesions, by 100% and 63.3%, respectively. CONCLUSION　Boanmycin is highly effective in a panel of models including the subcutaneous, orthotopic, intra-hepatic transplanted tumors, and hepatic metastases of murine colon carcinoma 26.

Three hundred and thirty eight patients with type 2 diabetes mellitus( DM) admitted from January 2006 to June 2009 and 50 healthy subjects (control group) were enrolled in the study. Platelet glycoprotein PAC-1 and CD62P levels were measured in both groups. Compared with control group, plasma PAC-1 and CD62P levels of DM patients increased significantly (P ＜0.01). Patients were divided into three groups according to their urinary albumin excretion rate ( UAER) . PAC-1 and CD62P levels in macroalbuminuria group (UAER≥200μg/min) were significantly higher than those in normoalbuminuria group( UAER <20μg/min) and microalbuminuria group (20μg/min≤UAER ＜ 200μg/min) (both P＜ 0.05). The results indicate that plasma levels of PAC-1 and CD62P, which reflect platelet activation, are the independent risk factors for diabetic nephropathy.

Dact proteins belong to a structure-related protein family. Recent studies have demonstrated that Dact proteins play an important role in tumorigenesis via modulation of wnt and TGF-p signaling. Delin-eation of the physiological function of Dact proteins would enhance our understanding of look for new strategy targets for cancer and suggests a potential strategy for therapeutic control of wnt and TGF-β signaling in canc-er.

Objective To investigate the relationship between lipocalintype prostaglandin dsynthase(L-PGDS) and visfatin in the lower extremity atherosclerotic plaque. Methods Collected from February 2014 to February 2016 in vascular surgery of our hospital 40 cases of femoral artery atherosclerotic plaque intima specimens (observation group), 20 cases of splenic artery, superior mesenteric artery samples (control group), the expression of visfatin and L-PGDS protein were detected by immunohistochemical staining, and the expression of visfatin and L-PGDS mRNA were determined by RT-PCR. Results The observation group visfatin protein expression was (121.42±11.07), significantly higher than the control group (72.07±12.81), and L-PGDS protein expression was (87.93±9.73), significantly lower than the control group (107.04±10.58), the difference was statistically significant (P<0.05). The relative expression of visfatin mRNA in the observation group was (0.321±0.024), which was significant higher than that of the control group (0.217±0.031), while L-PGDS mRNA was (0.203±0.018), significantly lower than the control group (0.314±0.029), the difference was statistically significant (P<0.05); The expression of L-PGDS protein was negatively correlated with the expression of visfatin protein in the plaque tissue (r=-0.617, P<0.05), visfatin mRNA and L-PGDS mRNA were negatively correlated (r=-0.645, P< 0.05). Conclusion Visfatin and L-PGDS in lower extremity atherosclerosis occurrence and development have an important relationship, both of them may play an antagonistic role.