The separation of teaching and test is not only important in improving teaching quality but also effective to the prevention of cheating in the exams,the improvement of the quality of exams and the correct checkout of the teaching effect.

Objective To study the cytotoxic activity of Chinese compound prescription (Xiaozheng Yiliu Decoction) combined with cisplatin on human ovarian cancer OVCAR/DDP cells and its molecular mechanism. Methods The xenograft model was established by injection of OVCAR/DDP cells. Forty nude mice were randomly allocated into four groupsincluding control group, cisplatin treatment group, low-dose Xiaozheng Yiliu plus cisplatin treatment group,and high-dose Xiaozheng Yiliu plus cisplatin treatment group.The apoptosis was determined by TUNEL analysis.The survivin expression was detected by RT-PCR analysis.Results The inhibit rates in cisplatin treatment group, low-dose Xiaozheng Yiliu plus cisplatin treatment group,and high-dose Xiaozheng Yiliu plus cisplatin treatment group,were(5.56±1.14) ％,(18.42±7.73) ％,and (31.59±12.58) ％,respectively,and there were significant differences among the groups(F=8.64,P＜0.01 ).The TUNEL analysis showed that the percentages of cell apoptosis were(2.35±1.04) ％,(4.56±1.61) ％,(12.42±3.68) ％,(21.59±5.17) ％ in four different groups,respectively.After treated by Xiaozheng Yiliu Decoction,the mRNA expression of survivin was found to be down-regulated by RT-PCR analysis. Conclusion Xiaozheng Yiliu Decoction could iucrease the cytotoxic activity of cisplatin on ovarian cancer OVCAR/DDP cells, which may be related to the down-regulation of survivin gene expression.

Objective To investigate the changes of gene expression profile in Beagle dogs' peripheral blood lymphocytes after acute irradiation.Methods Totally 20 male adult Beagle dogs were randomly divided into four groups including non-treatment blank control group and three radiation groups exposed to 0.5,2.0,and 5.0 Gy of γ-rays,respectively.Six hours after radiation,the peripheral blood were collected for lymphocytes isolation.Total RNA was extracted from the lymphocytes and analyzed by microarray hybridization.The differential gene profiles of radiation groups were analyzed by gene ontology (GO) analysis and kyoto encyclopedia of genes and genomes (KEGG) pathways analysis,and the alerted genes were further confirmed by the real time quantitative PCR (qRT-PCR) assay.Results Compared to the blank control group,the expressions of 308 genes in the radiation groups were perturbed over 2-fold of control,including 61 genes up-regulated and 247 genes down-regulated,which were mainly associated with immune response and cancer occurrence.The GO analysis indicated that the differential expressed genes were associated with cell connection,signal transduction,oxidation-reduction reaction and metabolism.The KEGG pathway analysis showed that some physiological and biochemical processes were involved,such as phagocytosis,tumor pathway,p53 signaling pathway,JAK-STAT signal pathway,cell differentiation,and proliferation,oxidative phosphorylation,glycogen dysplasia and other pathways.The microarray data of the alterations of apoptosis enhancing nuclease (AEN) and mitogenactivated protein kinase 13 (MAP3K13) gene expressions were further confirmed by qRT-PCR.Conclusions Exposure to different doses of acute γ-ray irradiation had significant impact on the gene expressions in Beagle dogs' peripheral blood lymphocytes and those differential expressed genes were related to a series of biological processes and pathways including immune response,metabolism and carcinogenesis.

Objective To observe the influences of nursing intervention in diabetics,guided by protection motivation theory (PMT),of their protection motivation of blood sugar monitoring,frequency of monitoring blood sugar and glycosylated hemoglobin,and level of their blood sugar and glycosylated hemoglobin.Methods A total of 100 diabetics eligible for this research were randomly divided into intervention group and control group,each with 50 cases.The patients in the control group were given routine nursing and the patients in the intervention group were given a further 6-month protection motivation theory-based nursing intervention.Changes in protection motivation of blood sugar monitoring,in frequency of monitoring blood sugar and glycosylated hemoglobin,and in index of blood sugar and glycosylated hemoglobin were compared.Results Before the intervention,comparison between scores in each dimension of protection motivation in blood sugar monitoring,frequency of monitoring blood sugar and glycosylated hemoglobin,and values of blood sugar and glycosylated hemoglobin between the two groups were not statistically significant (P ＞ 0.05).After the 6-month intervention in the intervention group,the susceptibility score was (6.530 ±0.952);the self-efficacy score was (9.462 ±0.617);blood glucose monitoring frequency was (24.126 ±2.712) times;glycosylated hemoglobin monitoring frequency was (2.080 ±0.868) times;fast blood sugar was (7.080 ± 0.824) mmol/L;glycosylated hemoglobin was (6.823 ± 0.158) ％;the scores and values in the control group were (3.820 ± 0.723),(6.729 ± 1.341),(18.528 ± 2.831) times,(1.812 ± 0.778) times,(8.488 ± 1.592) mmol/L,and (9.853 ± 0.899) ％,with significant differences (t =16.742,8.435,14.626,12.489,-15.537,3.395;P ＜ 0.05).Conclusions Nursing interventions guided by protection motivation theory can improve diabetics' protection motivation and promote behavior of blood sugar monitoring and their metabolic index up to standard.

Objective To evaluate the effects of mindfulness training on diabetes-related distress and quality of life in patients with type 2 diabetes mellitus, so as to provide evidence for nursing interventions. Methods A total of 90 inpatients with type 2 diabetes mellitus were randomly assigned to a control group and an observation group of 45 cases. The control group received routine health education, while the observation group received mindfulness training by the mindfulness training team composed of seven members including psychological counselors and nurse educators. Both groups were followed up for 6 months, and the scores of diabetes-related distress and quality of life were measured before and 6 months after the intervention. t-test was adopted to compare the difference between the two groups. Results After the intervention, the total score and scores of each dimension of diabetes-related distress in the observation group were (1.81±0.40), (1.99±0.45), (1.45±0.20), (2.01±0.27), and (1.51±0.21) respectively. Correspondingly, the scores of the control group were (2.07±0.24), (2.21±0.40), (1.61±0.19), (2.57±0.25) and (2.20±0.23), which were all higher than the observation group, with statistical differences (t=3.54, 2.32, 3.69, 9.68, 14.11;P<0.05). The scores of quality of life in disease dimension, physiological dimension, psychological dimension, social dimension and satisfaction dimension were (28.41±5.40), (21.05±4.16), (21.08±5.16), (19.04±3.30) and (22.84±3.79) in the observation group, which were all lower than those in the control group respectively [(36.08±5.20), (23.80±5.00), (24.11±5.22), (21.34±3.47), (24.64±3.50)], the differences were statistically significant (t=6.51, 2.69, 2.63, 3.06, 2.22;P< 0.05). Conclusions Mindfulness training can reduce the degree of diabetes-related distress and improve the quality of life in patients with type 2 diabetes mellitus.