Serum pepsinogen(PG)is an effective marker for evaluating gastric mucosa function and can be used as a supplement for screening and early diagnosis of gastric cancer. Recently,serum PG has become a hot spot of study in portal hypertensive gastropathy and functional dyspepsia. Serum PG detection is a noninvasive,simple and low cost investigation method with marked clinical value. This article reviewed the value of serum PG detection in warning gastric cancer and evaluating gastric mucosa function.

Objective T To investigate the effect of sirolimus (SRL) on the differentiation,development,and maturation of mice bone marrow-derived dendritic cells (DC),and the synergic effects of them in prolonging survival time of skin allograft.Methods (1) DC of C57BL/6 mice were derived from bone marrow cells upon culture with SRL. The expression of CD11c,CD86 and major histocompatibility complex (MHCⅡ) molecules was assessed with or without lipopolysaccharide (LPS) stimulation by flow cytometry; (2) The capacity of DC administrated by SRL to stimulate allogeneic T lymphocyte proliferation was examined by mixed lymphocyte culture (MLR); (3) A skin transplantation model was established with the recipients BALB/c mice and the donor C57BL/6 mice. Recipients were divided into control group (there was no administration before skin transplantation),immature DC group (injection of donor C57BL/6 mice immature dendritic cells 2?10 6 via tail vein before skin transplantation),SRL group (receiving oral SRL 3 mg/kg every day for 7 days before skin transplantation),combined group (receiving an injection of donor C57BL/6 mice immature DC via tail vein and of oral SRL before skin transplantation),and isogeneic group (in which the donors and recipients were both BALB/c mice and there was no administration before skin transplantation). Survival time and histological changes of skin allograft were observed in different groups.Results (1) CD11c expression on the DC in the presence of SRL was slightly decreased,but CD86 and MHCⅡ molecules significantly decreased,and SRL treatment could resist the stimulation of LPS; (2) MLR revealed that DC administrated by SRL could inhibit allogeneic T lymphocyte proliferation; (3) SRL treatment in combination with donor immature DC before transplantation could alleviate inflammation and prolong survival time of skin allograft in mice.Conclusions SRL does not alter differentiation but inhibit the maturation of DC. Sirolimus can cooperate with immature DC to prolong survival time of skin allograft in mice.

Objective:To evaluate the effect of nutritional status on postoperative outcomes for patients with gastric cancer. Methods:Data of 353 gastric cancer patients at Xinjiang Medical University Affiliated Tumor Hospital between January 2013 and October 2014 were collected prospectively. Preoperative nutritional status was evaluated using Nutrition Risk Screening 2002 (NRS 2002). Postoperative complication rates were compared among different preoperative nutritional status. Results:On the basis of NRS 2002, the morbidities of patients with and without malnutrition risk were 47.0%(77/164) and 31.2%(59/189), respectively (P=0.002). Among the patients with an NRS score of at least 3, the complication rate was significantly lower in the group with preoperative nutrition than in the group of patients without preoperative nutrition support (P=0.013). NRS 2002 was a significant predictor of postoperative complications (P=0.039, OR=1.634, 95%CI:1.025-2.606) on the basis of multivariable logistic regression analysis. Conclusion:As a nutritional evaluation tool, NRS 2002 may predict postoperative complications for gastric cancer patients.

AIM: To investigate the effect of Qingjiening(QJN) on cytokines in sheep red blood cell(SRBC)-immunized mice. METHODS: After immunization of mice with SRBC, the effect of QJN o n IL-1、IFN-?、IL-2 in mice was observed, the IFN-? level was measured by macrophage NO - 2-release assay, the IL-1 level was measured by thymocyte a ssay, the IL-2 level was measured by mitogen activated lymphocytoblast assay. RESULTS: QJN can significantly inhibit mice to secrete IL-1、IFN- ? and IL-2. CONCLUSION: The immunosuppressive activity of QJN may be associate d with inhibition of immunocyte to secret IL-1、IFN-? and IL-2.

AIM: To observe the effects of some component of Chinese herbs for external use on proliferation of human umbilical vein endothelial cells (HUVEC) and investigate the mechanism of promoting tissue repair. METHODS: The method of MTT was used to examine the effects of Rg1, Rh1, perlolyrine, cinnamyl aldehyde, muscone, astragalus polysaccharin (APS), velver antler polypeptide (VAP) and soluble extract of boswellia carterii birdw (BCB) on proliferation of HUVEC. RESULTS: APS did not promote proliferation of HUVEC at 9.75 mg/L-2.5 g/L; Rh1 promoted proliferation of HUVEC at 1.94 mg/L-0.5 g/L (P

Objective To analyze the incidence factors between accompanies and mwses in pediatric transfusion clinic, and find out the countermeasures. Methods 75 conflicts occurred between August 2004 to August 2008 were analyzed and made a contrast. Results The elements for the conflict were as follows: nurses, accompanies and patients. Conclusions The nurses should improve the emergency cop-ing abilities, communication skills, technical skills, supporting psychological nursing for the patients and the accompanies. All these are effective measures to avoid conflicts.

Objective:Based on the model of human umbilical vein endothelial cell(HUVEC) treated by tumor necrosis factor-alfa(TNF),We investigate the effects of muscone on polymophonulear leukocytes(PMN)-HUVEC adhesion and its adhesion molecules(CAMs) expression.Methods:Confocal system was used for identifying cultured HUVEC,MTT assay for its activity,Rose Bengal Staining for PMN-HUVEC adhesion,and fluorescent-immunocytochemistry techniques for CAMs expression.Results:After HUVEC treated by TNF,the adhesion between PMN and HUVEC increased dramatically(P

AIM: To investigate the anti-metastasis effect of weimaining, extracted from fragopyrum cymosum meissn, a Chinese medicine, on murine Lewis lung carcinoma (3LL). METHODS: The anti-metastasis effect of weimaining in vivo was detected in the grafting lung metastasis model of murine Lewis lung carcinoma. The effects of the drug on the expression of CD34 and E-cadherin were investigated by immunohistochemical staining and RT-PCR. RESULTS: Weimaining effectively inhibited the lung metastasis of 3LL at a concentration of 250 mg?kg -1?d -1, significantly suppressed the expression of CD34 and increased the expression levels of E-cadherin protein and mRNA in 3LL cells. CONCLUSIONS: Weimaining inhibits the metastasis of murine Lewis lung carcinoma (3LL) in vivo via increasing the expression of E-cadherin and decreasing microvessel density of tumor tissue.

AIM: To investigate the effects of Astragalus polysacharin(APS) on human fibroblast and human umbilical vein endothelia cell (HUVEC) proliferation, as well as its acts on adhesion between white cells and HUVECs. METHODS: Human fibroblasts from distal and proximal skin away the ulcer were cultured as normal fibroblasts(NF) and wounded fibroblasts(WF). MTT assay was used for detecting cell proliferation, Rose Bengal staining and fluorescence immunohistology assay were used for examining the adhesion of human polymorpho-nuclear cell(PMN) and TPH-1 to HUVECs. RESULTS: 2 44-156 mg/L APS promoted WF proliferation, and 2 44-39 mg/L APS also promoted NF proliferation, but it did not show any proliferating effect on HUVECs. APS inhibited the adhesion of PMN or TPH-1 to HUVECs induced by tumor necrosis factor(TNF). At 25-100 mg/L, it also inhibited both VCAM-1 and ICAM-1 expression in HUVECs induced by TNF. Treatment with APS for 12 h also inhibited CD44 expression in HUVECs. CONCLUSION: APS shows mitogenic activity on both human normal and wounded fibroblasts. It also exerts anti-inflammation effects by inhibiting adhesion molecule expression and adhesion of white cells to HUVECs. [

Objective To explore the clinical value of perfusion index (PI) in treatment of full-term infants with shock.Methods Totally 52 full-term neonates were selected and divided into mild shock group (n =16),moderate shock group (n =28) and severe shock group (n =8) according to neonatal shock score,and PI value was detected at the time points of visiting doctor,24 h and 48 h after treatment.Meanwhile,48 normal full-term newborns in the same period were selected as control group,and the PI value was measured at birth,postnatal 24 h and 48 h.The PI value and prognosis was analyzed.Results The PI values at birth,postnatal 24 h and 48 h in neonatal shock group were significantly lower than those in control group (P ＜ 0.05).With the increase of the severity of the disease,the PI value of neonates with shock decreased significantly,and there were no significant difference among the four groups after anti-shock treatment (P ＞ 0.05).Conclusion The PI value of full-term neonates with shock significantly decrease.