Objective:To observe vox acoustics features of patients of lung Qi deficiency,understand the influence of different extents of lung Qi deficiency on phonation,and expound the relationship between lung Qi state and phonation. Methods:Apply computer vox analysis system to test the vox sustainable vowel signal(i) of 65 cases of lung Qi deficiency and 20 cases of healthy persons respectively,observe and analyse their acoustics parameters(basic frequency,micro bother of frequency,slight bother of swing and standard yawp energy) of natural sound lowest bourdon and comfort sound and natural sound most alt. Results:For lung Qi deficiency patients,the basic frequency of 3 different phonations is lower than healthy persons,whose lowering trend increases with degrees of lung Qi deficiency,among which,under lowest bourdon of natural sound,there's marked difference of basic frequency between middle and severe lung Qi deficiency and healthy adults(P

Objective To detect the expression of human telomerase reverse transcriptase (hTERT) mRNA in the melanoma, and to analyze the relationship between the expression and subtypes and clinicopathological features of melanoma. Methods Expression of hTERT mRNA was detected by real-time quantitative PCR in 64 cases of melanoma and 30 cases of nevus. SPSS 17.0 software was used to analyze the relationship between hTERT mRNA expression and clinical pathological features of melanoma. Results The relative expression of hTERT mRNA in melanoma tissues was higher than that in nevus tissues [(52.43±5.42) vs (21.38±3.73), t= 4.72, P= 0.000]. The expression of hTERT mRNA in melanoma had no significant correlation with age, gender, ethnicity (all P> 0.05), but had relationship with subtypes, lymph node metastasis, Clark classification (all P< 0.05). The expression of hTERT mRNA in mucosal melanoma was significantly higher than that of acral and non-acral melanoma (t= 7.71, P= 0.001), while the expression of acral and non-acral melanoma had no difference (P> 0.05). Conclusions The expression of hTERT mRNA in melanoma is high, especially in mucosal melanoma. hTERT may play an important role in the occurrence and development of melanoma.