Objective To understand the development of psychosocial adaptation and life quality of patients with visual impairment, as well as their relationship. Methods Researches about impact of psychosocial adaptation on life quality of patients with visual impairment underwent document retrieval and the results went through analysis. Results Cataract, glaucoma, retinal detachment, diabetic retinopathy, age-related macular degeneration caused different degree of visual impairment, furthermore influence physical, psychosocial adaptation and life quality of these patients. Some scales were compiled by some scholars at home and abroad to evaluate the influence degree of these factors, but tools to test psychosocial adaptation lacked. Conclusions Researches on quality of life for a single eye impairment are more than those on the relationship between psychosocial adaptation and quality of life. At the same time, it is necessary to develop a tool to measure psychosocial adaptation of visual function impairment.

OBJECTIVE:To provide reference for the further implementation of national essential medicines. METHODS:The drugs consumption data of outpatients and inpatients in our hospital from Jan. 1 to Jun. 30,2014 were statistically analyzed. RE-SULTS:Variety numbers of national essential medicines in our hospital occupied 37.38%(228/610) in total drugs variety numbers of the whole hospital,consumption sum of national essential medicines occupied 9.80%(817.02 million yuan/8 338.12 million yu-an) in all drugs consumption sum. The first 20 national essential medicines listed in consumption sum were mainly anti-cancer drugs and water,electrolyte and acid-base balance regulation drugs. CONCLUSIONS:In the utilization of national essential medi-cines,variety proportion and consumption sum ratio in our hospital are relatively low. It is suggested to make clear the use require-ments of national essential medicines in cancer hospitals to make more reasonable and scientific selection of anti-tumor medicines in National Essential Medicines List and reasonably increase the assistant medicines related to the treatment of neoplastic diseases;hos-pitals should take effective measures to promote the usage rate of national essential medicines.

Objective To analyze the characteristics of bone marrow metastasis in large intestine carcinomas.Method [WT5”BZ]The systematic bone radionuclide images and clinical feature of pathology in 423 postoperative patients with colorectal carcinomas were analyzed retrospectively.Results Bone marrow metastases was confirmed in 191 cases accounting for 5 5% (191/3 454) of the patients admitted during the same period. 7 5% (146/ 1 934 ) of the rectal cancer cases had bone metastasis, which was significantly higher than that of 2 9%(45/1 520) in colon cancer (? 2=34 3, P 0 05). 108 cases had local metastasis, while 83 cases had systematic multiple metastases.Conclusions Bone marrow metastasis of large intestine carcinoma occurred most often secondary to liver and lung metastases. The bone metastasis in rectal carcinoma is more often than colonic carcinoma.

Objective To investigate the association of the two single nucleotide polymorphisms(SNPs) rs1386494 and G1463A of tryptophan hydroxylase 2 gene with depression in Yunnan Han population. Methods A case-control study Was designed by collecting 102 patients and 102 controls.Polymerase chain reaction-restriction fragment length polymorphisms(PCR-RFLP)technique Was used to detect the rs1386494 and G1463A polymorphisms.All patients were evaluated with Hamilton Rating Scale for Depression(HAMD),and scores were compared according to different genotype.Results 1.There were differences in genotype and allele frequency of rsl386494 in Yunnan Han population(X~2=4.300,P=0.038;X~2=4.067,P=0.044).The A allele frequency of rsl386494 in controls Was higher than in patients(P=0.044).2.No genotype of G1463A polymorphism was observed in all samples.3.No significant association genotype of rsl386494 with scores of HAMD Was observed(F=2.461,P=0.120).Conclusion The polymorphism of rsl386494 may be associated with the vulnerability of Yunnan Han population,and the A allele maybe the protective gene for depression.

OBJECTIVE:To optimize the formulation of Budesonide sustained-release tablet. METHODS:Using the cumula-tive releases in 2,4,8 h as investigation indexes,central composite design-response surface method was used to optimize the amount of hydroxypropylcellulose L(HPC-L),amount of soybean phosphatides,and filler(fixed total 200 mg)lactose- micro-crystalline cellulose mass ratio in the formulation of Budesonide sustained-release tablet,and the verification test was conducted. The release behaviors of prepared sustained-release tablet and original preparation in pH 7.2,7.0,6.8 phosphate buffer were com-pared. RESULTS:The optimal formulation was as follow as budesonide of 9 mg,HPC-L of 46.49 mg,soybean phosphatides of 9.23 mg,filler lactose-microcrystalline cellulose mass ratio of 1:2.9;the cumulative releases in 2,4,8 h were 21.9%,50.1%, 99.5%,the relative errors with predicted values (22.0%,50.0%,98.5%) were 0.45%,0.20%,1.02%(n=3),respectively. Compared with cumulative release of original preparation,the f2 was higher than 50. CONCLUSIONS:Budesonide sustained-re-lease tablet is successfully prepared,which shows similar release behavior to original preparations.

Objective To revise the Research Capacity Self-Rating Scales of Nursing Staff and test its reliability and validity.Methods Based on the definition of scientific research capacity,the initial dimensions and items of Research Capacity Self-Rating Scales of Nursing Staff was formed by literature review,semi-structured interview and experts evaluation.The content validity was evaluated by experts,and the face validity by nursing staffs.600 nurses were investigated by convenient sampling.The reliability and validity and discrimination of the scale were tested.Results Research Capacity Self-Rating Scales of Nursing Staff were selected in 7 dimensions including the capacity of find problem,research design,scientific research practice,innovation,information retrieval,data processing and writing.Scale-level Content validity index was 0.94.The Cronbach alpha coefficient of Research Capacity Self-rating Scales of Nursing Staff was 0.964,the Cronbach alpha coefficients of the seven dimensions were 0.771-0.975,split-half reliability was 0.885.Seven factors were extracted by using principal factor analysis,cumulative contribution was 71.002％.The correlation coefficient between the Scientific Research Capacity Self-rating Scales of Nursing Staff and General Self-Efficacy Scale was 0.649 (P=0.000).The discrimination was better.Conclusions The Research Capacity Self-rating Scales of Nursing Staff revised shows good reliability and validity.It can objectively evaluate scientific research capacity of nursing staff and provide scientific reference for further improving the research capacity of the nursing staff.

Objective To screen the Doc 1R gene recombinant plasmid by use of colony PCR. Method The recombinant colonies were transfered into the PCR reaction mixture. The PCR primers were used for constructing mouse Doc 1R genomic sequence. Result Among the 5, 3 positive strips in the size of 1 500 bp were visible, which were the same as the Doc 1R gene fractions in terms of their sizes were screened as positive clones. The positive colony were further confirmed by double digestion and DNA sequencing. Conclusion Colony PCR is a simple, efficient and reliable technique for screening the recombinant.

Objective:To explore the effects of eight-week group positive psychotherapy intervention on de-pression associated self-efficacy and subjective well-being in female nursing students.Methods:Totally 74 female nursing students with mild to moderate depression [the score of Beck Depression Inventory (BDI-Ⅱ)was 14 to 28]were selected from three nursing colleges of Changsha.They were randomly divided into two groups,40 in the control group and 34 in the intervention group.Samples in the intervention group were given the eight-week group positive psychotherapy,while the control group was given nothing (the control group could receive the intervention when the intervention was proved to be effective or the control group needed treatments).The General Self-efficacy Scale(GSES)and Index of Well-being(IWB)were used to get the outcome of female nursing students'self-effica-cy,subjective well-being at baseline,the time after the intervention,3 month follow-up,6 month follow-up.Results:The interactive effect between group and time of self-efficacy,subjective well-being were statistically significant (Ps<0.05).Separate analyses showed that at baseline,there was no significant difference between two groups in all variables (Ps >0.05).At the end of the intervention,3 month follow-up and 6 month follow-up,the GSES score and IWB score in the intervention group were higher than those in the control group (Ps <0.05).Conclusion:Eight-week group positive psychotherapy could improve self-efficacy and subjective well-being of female nursing students who have mild to moderate depressive symptom.This intervention also has a long-term effect.

Objective To investigate the phagocytosis function of cigarette smoke extracts (CSE)on the NR8383 cells. Methods The concentration of CSE and the optimal time was defined by cell counting kit-8 assay, Annexin V/PI cell apoptosis assay and CFSE cell proliferation assay. The cell was gained after exposed to the different concentration of CSE for 24 h and mixed with fluorescein-labeled Escherichia coli in 37℃ for 2 h. The fluorescence intensity was used to assay the phagocytosis function of NR8383 cells.Results The phagocytosis function of NR8383 cells may be changed by the concentration of CSE. In the concentration of 100 μg/ml, the phagocytosis function of NR8383 was enhanced 0.5 times than the normal cell when NR8383 cell was exposed to CSE, and the specific activity is the highest. When NR8383 cells were exposed to CSE and LPS, the phagocytosis function of NR8383 cells was enhanced 2 times than the normal cell. In the concentration of 200 μg/ml, the phagocytosis function of NR8383 cells was damaged, the rate of apoptosis is the 54. 1%. Conclusion Low concentration of CSE enhanced the phagocytosis function of NR8383 cells, but high concentration of CSE damaged the phagocytosis function of NR8383 cells. This study reveals a new role of CSE as an activator of macrophage function.

Objective To investigate the effect of IL-1β-511 genetic polymorphism on postoperative analgesia with fentanyl. Methods Two hundred and fifty ASA Ⅰ or Ⅱ patients of Han nationality (native of Henan province) aged 20-50 yr undergoing elective abdominal total hysterectomy or myomectomy under general anesthesia were enrolled in this study. The polymorphic sites of the IL-1β-511 allele were analyzed by polymerase chain reaction-restriction fragment length polymorphism. The patients were assigned into 3 groups according to their genotypes: group wild homozygote; group mutation hetorozygote and group mutation homozygote. Anesthesia was induced with midazolam, remifentanil, propofol and succinylcholine and maintained with propofol, remifentanil and atracurium. The patients were mechanically ventilated after tracheal intubation. The pain was assessed using VAS score after the patients recovered from anesthesia. When VAS score was ＞ 3 the patients were given fentany120 μg every 5 min until VAS score decreased to ≤ 3. PCIA with fentanyl was then started. The PCIA solution contained fentanyl 1.0 mg and droperidol 5mg in 100 ml of normal saline. The PCA pump was set to deliver a background infusion of 0.5 ml/h and a bolus dose of 2 ml at 5 min lockout interval. The VAS score was maintained at ≤3.The amount of fentanyl consumed during 24 h of PCIA was recorded. Results There was no significant difference in the amount of fentanyl consumed during the 24 h PCIA among the 3 groups. Conclusion IL-1β-511 genetic polymorphism is not the factor contributing to the individual variation in the patient' s response to postopertive analgesia with fentanyl, indicating that the pain within 24 h after operation is not related to the inflammatory factors.