Objective To investigate the difference of TCM body constitution betweenpeople of the Shimian county and Chengdu city. Methods 200 sub-health patients in clinic of Shimian TCM Hospital were selected randomly. Another 200 sub-health patients in clinic of the Second Sichuan Province Hospital of TCM wererandomly selected. The two groups were identified with TCM body constitution based on the clinical symptoms, tongue, pulse condition, etc. Comparisons on body constitution were made between the two groups. Results In Chengdu, patients withbalance constitution and Qi deficiency constitution (respectively have the proportion of 33.0% and 20.0%)were significantly higher than thoseof Shimian County(the proportion of the two wererespectively 4.0% and 6.0%);while Shimian County have a significantly higher ratio of phlegm dampness and blood stasis constitution(43.5% and 13.0%)than those in Chengdu city(the proportion of the two wererespectively 8.0%and 4.0%), P<0.01. Conclusion Because of different regions, the population constitution ratio wasdifferent, so the disease tendency wasalso somewhat different. Therefore, physicians should give full consideration to the regional factors in diagnosing and treating patients.

Purpose:To investigate the treatment effect and toxicity of regimen containing ifosfamide (IFO) and etoposide (VP16) on relapse ovarian carcinoma.Methods:IFO 2.0 in 500 ml normal saline day 1—5, VP16 100 mg in 500 ml normal saline d1,3,5 Results:The overall response rate was 38.5%, mylosuppression and alopecia were the most common toxicities. Leukopenia rate was 71.4%, alopecia rate was 100%, gastro intestinal and nervous toxicity were mild. Conclusions:Combination of IFO and VP16 was very effective on relapse ovarian carcinoma with mild toxicity and should be widely used.

Objective To analyze the relationship between WT1 gene polymorphism rs16754 and prognosis of acute myeloid leukemia (AML) by meta-analysis. Methods Studies published in PubMed, CNKI, WanFang and CBM database were searched by using the search terms 'WT1', 'Polymorphism' and 'Leukemia, Myeloid, Acute', respectively. Deadline was December 1, 2015. Results A total of 11 English articles with a total of 2 789 patients were included. Meta-analysis showed that there was no association between polymorphism rs16754 and complete remission of AML patients (RR=1.02, 95 % CI: 0.99~1.06, P=0.20), overall survival(OS) (HR=0.68, 95 % CI: 0.45~1.02, P=0.06), 5-year OS (RR=1.10, 95 % CI:0.90~1.34, P=0.37) or relapse-free survival (RFS) (HR=0.80, 95 % CI: 0.54~1.19, P=0.27). Conclusion There is no correlation between WT1 gene polymorphism rs16754 and the prognosis of AML.

Recently, along with the harmful factors in air in-creasing, the incidence of respiratory system diseases has been gradually rising. Therefore, more studies are focused on impro-ving the prevention and treatment on respiratory system diseases. Current researches have shown that microRNAs ( miRNA ) con-tribute to the prevention and treatment on respiratory system dis-eases, including virus infectious respiratory disease, pulmonary fibrosis, asthma, COPD, pulmonary tuberculosis and lung canc-er. The up-regulation and down-regulation of microRNAs could effectively regulate the biological process of these diseases. The review analyzes the recent years′study results of respiratory sys-tem diseases and microRNAs in depth, and discuss the role of microRNA in the treatment and prevention of respiratory disea-ses. Moreover, in this review we can provide new knowledge a-bout respiratory diseases and development of more potent drug targets for the treatment and prevention of respiratory diseases.

Objective To observe the effects of elevated temperatures(37~44.5℃)on the cell counts, hemolysis and osmotic fragility of stored red blood cells.Methods After 7~35 days of storage at 4℃, erythrocyte suspensions were heated by incubation at 37℃ and 44.5℃ for 30 min in an incubator and then examined for cell counts, hemolysis and osmotic fragility.Results Red blood cell, white blood cell and platelet counts, hemoglobin and potassium were unchanged following the heat treatment. In the osmotic fragility test, hemolysis remained within normal limits. Hemolysis of groups incubated at 37℃ and 44.5℃ began at (4.41?0.38)g/L and (4.3?0.2)g/L respectively while a complete hemolysis occurred at (3.42?0.36)g/L and (3.4?0.1)g/L , showing no significant difference compared to the room temperature control (4.5?0.18)g/L and (3.6?0.1)g/L. There was no significant distinction in the proportions of smooth spherocyte among the samples incubated at different temperatures, except the samples stored for 35 days, probably due to the long storage period.Conclusions Heating at temperatures between 37～44.5℃ for as long as 30 min does not cause hemolysis or other damage to red blood cells.

Objective:To investigate the effect of cPLA 2(cytosolic PLA 2, 85-kD PLA 2) on the signaling pathways of IL-1?、IL-6 release in LPS induced Hela cells.Methods:①LPS stimulated Hela cells were exposed to the complex of cPLA 2 initiation site-directed antisense oligonucleotide and liposome in the indicated doses and time, and then,analyzed the changes of cPLA 2 both in mRNA and in protein by RT-PCR and Western blot.②At the same time, the supernatants were collected to detect the level of IL-1? and IL-6 under radioimmunoassay(RIA).Results:①The transfection led to a dose-dependent decrease in cPLA 2 protein, while the change of mRNA is not notable.②The levels of IL-1?、IL-6 in the transfected cells were reduced following the repression of cPLA 2 compared with that of LPS added only.Conclusion:cPLA 2 probably plays an important role in the signaling mechanisms on IL-1?、IL-6 release in LPS-induced Hela cell. [

Objective To investigate the effect of metronidazole and tinidaxole in breast milk on infants and their concentrations. Methods The concentration of metronidazole and tinidazole inbreast milk was assayed by high performance liquid chromatography(HPLC). Results The pharmacokineticparameters(mean?s) t max(h), t 1/2ke of Metronidazole(20 mg/kg, n=8 ) and Tinidazole (13 mg/kg,n=7) in lactation women infused with a single dose were(1.7?1.0)h、(20?5) mg/L and (1.3?0.6)h、(17?3) mg/L respectively. The total amount of metronidazole and tinidazole ingested by the infant via breast milk were 3.02 mg/kg/day and 2.58 mg/(kg?d) respectively. which were 1/5~1/10. and 1/6~1/8 of the routine therapeutic dose for infants.

Extract of Ground Beetle was found to affect rat rheology. when given orally to rats for 10 consecutive days, it resulted in the lowering of packed corpuscular volume, high and low whole blood shear viscosity, RBC aggregation and rigidity indices and sedimentation rate,but the constant of SR equation was raised and without effect on Plasma viscosity and fibrinogen content. These results suggested that the effect of ground beetle extract is by way of its action on RBC

Objective To evaluate the antibacterial activity in vitro of Galla Chinensis ethanol extractant(GCEE)against S.epidermidis,and the change of cell morphology of S.epidermidis.Methods Deterimination of antibacterial activity of GCEE against 124 strains S.epidermidis was performed by using agar dilution method,and the changes of cell merphalogy of S.epidermidis was examined by microscope.Results The MIC90 of GCEE against 43 strains of MRSE(methicillin-resistant staphylococcus epidermidis)and 81 strains of MSSE(methicillin-sensitive staphylococcus epidermidis)were 0.288 and 0.144 mg/mL respectively.Under microscope,the cells of S.epidermidis were expansion by degrees,with the edge of cell of S.epidermidis irregular.The substances in cells of S.epidermidis were disappear final.Conclusion Galla Chinensis ethanol extractant had strong antibacterial activity against 124 strains of S.epidermidis,and the cell morphology of S.epidermidis changed.

Objective To evaluate the antibacterial activity of Galla chinensis etc.14 kinds of TCM against 112 strains of Staphylococcus aureus in vitro. Method Deterimination of antibacterial activity of Galla chinensis etc.against 112 strains of Staphylococcus aureus was performed by using new agar dilution method. Results The antibacterial efficiency of Galla chinensis, Forsythia suspensa and Acacia catechu (L.) Willd against 112 strains Staphylococcus aureus was good, and the MIC90 was 0.102, 0.244, 1.19 mg/mL respectively. Conclusion Galla chinensis etc. 14 kinds of TCM had strong antibacterial activity agsinst 112 srains of Staphylococcus aureus.