Objective To explore effectiveness of combination of traditional Chinese medicine and western medicine therapy for patients with Barrett esophagus (BE).Methods Patients with BE were divided into the control group and observation group according to random number table.The observation group was received treatment of com-bination of argon plasma coagulation (APC)and traditional Chinese medicine,while the control group was received treatment of APC alone.Clinical effectiveness was observed at 3,6,12,18months after treatments,recurrence rate was observed 6,12,18months after treatments.Results Clinical effectiveness were significantly higher in the observation group than in the control group 3,6,12,18months after treatments (χ2 =4.71,3.55,7.28,5.10,all P <0.05). Recurrence rate were significantly lower in the observation group than in the control group 6,12,18 months after treat-ments (χ2 =5.84,6.51,all P <0.05).Conclusion Combination therapy of traditional Chinese medicine and west-ern medicine shows confirmed effects to BE.Low recurrence rate in addition makes it become a promising clinical therapy in treatment of BE.

Objective To observe the influence of post-operative swallowing painwith Disposcope endoscope or Macintosh laryngoscope in patients with difficult visible glottis. Methods Thirty-six patients scheduled for elective surgery under general anesthesia whose glottis was not visible underMacintosh laryngoscope (according to Cormach-Lehane Grading of laryngoscopic view) were randomized into 2 groups (n = 18 each):Disposcope endoscope group (group D) and Macintosh laryngoscope group (group M). The rate of glottis exposure and successful intubation, swallowing pain at 30 min, 1, 3 d after extubation were observed. Results Compared with group M , the successful rate of glottis exposure and first intubation was significantly higher than thatin group D (P < 0.05). Swallowing pain VAS was higher in group M than in group D at 1d afterextubation (P < 0.05). Conclusions Compared with Macintosh laryngoscope , Disposcope endoscope in patients with difficult visible glottis could induce higher successful rate of glottis exposure and first intubation , shorter time of intubation and lower VAS of swallowing pain at 1d after extubation.

BACKGROUND:Currently, the implant surface is optimized to promote the osseointegration of artificial dental implants, which has become a research focus in recent years. But the femtosecond laser is less reported to modify the titanium surface so as to promote fibroblast proliferation and differentiation. OBJECTIVE: To observe the effect of femtosecond laser on the proliferation and differentiation of human periodontal ligament fibroblasts on titanium surface. METHODS: Human periodontal ligament fibroblasts were respectively inoculated on different titanium surfaces of the three groups (femtosecond laser group, TiO2 particle blasted group and pure titanium group). Then differentiation and proliferation of human periodontal ligament fibroblasts were observed. RESULTS AND CONCLUSION:There was a significant difference in the surface roughness among these three groups, and compared with the pure titanium group, the attached cels were more in the femtosecond laser group and TiO2 particle blasted group. mRNA expressions of RUNX2 and OSX were highest in the femtosecond laser group folowed by TiO2 particle blasted group and pure titanium group in order. These findings indicate that pure titanium treated with femtosecond laser has fine biological property and may be more feasible for human periodontal ligament fibroblasts to attach, grow and proliferate.

Objective To observe the clinical efect of Weisu particle combined with Mosapride in the treat-ment of the functional dyspepsia .Methods According to the digital table ,64 patients were divided into two groups in random,32 cases were treated by Weisu particle combined with Mosapride in treat group ,33 cases treated by Mosa-pride in control group .Both have a course of 4 weeks treatment .Results The efective rate of the treat ment group′was/96.87%which was better than that of the control group′(s 68.75%)(χ2=4.68,P<0.05).Conclusion The thera-peutic method of Weisu particle combined with Mosapride have a better clinical effect than that of domperidone only used .

Objective To observe the application of angiotensin Ⅱ receptor antagonist valsartan in reducing minim albuminuria in earlier period of the diabetes and kidney disease.Methotis 36 patients Who administered angiotenain Ⅱ receptor antagonist valsartan 80～160nag,qd,for 8 weeks.Compared with symptoms before the treatment,the blood pressure,the amount of the urinary albuminufia,and observed the change of the empty stomach blood sugar,the saccharified hemoglobin,the triglyceride,the total cholesterol,the endogenous creatinine clearance rate,etc.Results After the treatment,the urinary albumin drops obviously(P＜0.01),While the blood pressure,the empty stomach blood sugar,the saccharified hemoglobin,the triglyceride,the total cholesterol and the endogenous creatinine clearance rate have no obvious change.There is no obvious side effect during therapeutic process.Conclusion The application of angiotenain Ⅱ receptor antagonist valsartan can reduce the amount of the urinary albumin,is the effective way in treating the earlier period of the diabetes and kidney disease.

Objective To study the short-term therapeutic effects of adeno-associated viral gene transfer of sarcoplasmic reticulum Ca2+-ATPase (SRCA2a) on chronic heart failure (CHF). Methods The rats were divided into four groups: control group, CHF group, CHF+eGFP group and CHF+SRCA2a group. CHF rats were obtained by creating descending aortic constriction. 0.9% sodium chloride solution, recombinant adeno-associated virus carrying enhanced green fluorescent protein (eGFP) gene, and recombinant adeno-associated virus carrying SRCA2a gene, were respectively injected into pericardium of heart failure rats in different groups by intrapericardial injection via trans-diaphragmatic approach. 10 days after gene transfer, hemodynamic parameters, the protein expression of SRCA2a and the SRCA2a activity were measured and analyzed respectively. Results 10 days after gene transfer, the protein expression and activity of SRCA2a were increased by 80.7% and 89.9% respectively, but still lower than those in the control rats. The heart function was also improved along with the increase of SRCA2a expression and activity. The left ventricular systolic pressure and the maximal rate of decline of left-ventricular pressure in CHF+SRCA2a group were similar with those of the controls. The left ventricular end-diastolic pressure and the maximal rate of rise of left-ventricular pressure were much improved, but did not reach the level of the controls. Conclusions SRCA2a protein levels and activity are significantly decreased in CHF rats. Adeno-associated viral gene transfer of SRCA2a can improve the function of the heart in short term.

Objective To study effect of diclofenac sodium on content of prostaglandin E 2 KC/MC ratio and hyperpigmentation of the full-thickness skin autografts. Methods Content of PGE 2 was determined by radioimmune analysis method, and kinetic changes of KC/MC ratio were observed with histological and histochemical methods. Results In the operation control group, increase of KC/MC ratios was accompanied by of PGE 2 content at the early stage of the experiment; and the KMR became smaller than that in normal control group at the end of the experiment. While in the diclofenac soduim group, KMR was reduced due to the less damage of MC at the early stage, with decrease of PGE 2. Accordingly, KMR became larger at the late period of the experiment, and color of the autografts became lighter. Conclusion Application of diclofenac sodium on the grafts just after transplantation may reduce the damage of inflammation to the epidermal melanin units, control their proliferation and decrease the value of KMR, which lightens the color of the grafts.

Objective To compare the effective difference of the flying needle with intradermal needle among the shu-acupoint group , the estazolam group and the non- point acupuncture group. Methods Three undred and fifteen patients who met the inclusion criteria were randomly enrolled into three groups for the insomnia severity index (ISI) and rating changes detection. After 2-week treatment,the patients were followed up at 4 weeks later. Results The ISI decreased gradually at 1 week, 2 weeks, and 4 weeks after treatment.The therapeutic effects of the three groups were all better than ever before at 1 week, 2 weeks, and 4 weeks after treatment (P<0.05). The follow-up therapeutic effect of the flying needle acupuncture group was better than that of the estazolam group and of the non-point acupuncture group (P<0.05). The curative effect of flying needle acupuncture is better than that in the other two groups (P < 0.05). Conclusion The subjective sleep quality of insomnia patients received flying needle with intradermal needle in shu-acupoint is beter than that of insomnia patients of the Estazolam group and of the non-point acupuncture group.

BACKGROUND:Tissue-engineered bone, as an emerging method, is used to repair bone defects, but it is difficult to be used widely because of no nutrient metabolism of implants. Pre-vascularized tissue-engineered bone research is performed for this limitation. Forward-looking and procedural vascular construction prior to artificial grafting is perspective to supply the nutrient metabolism of implants.
OBJECTIVE:To analyze the research results and developmental tendency of pre-vascularized bone engineering in vitro using a multilevel analysis.
METHOD:Literature search was performed in CNKI database for Chinese literatures and PubMed database for English literatures from 2000 to 2012. The key words included“tissue-engineered bone, vascularization, implant, osteoblasts, endothelial cel s, co-culture”in Chinese, and“bone engineering, endothelial cel s, osteoblast, implant, cel s co-culture”in English. A review addressing pre-vascularized tissue-engineered bone was completed based on retrieved literature classified according to bone physiological studies, in vitro experimental research and material research.
RESULTS AND CONCLUSION:Total y 60 articles were included in result analysis. Studied have documented that microvascular regeneration in normal bone tissue has an important role in osteogenesis regulation. Current bone tissue engineering research is to simulate this physiological process of vascular regeneration in the human body, and lots of in vitro studies are designed to complete this angiogenic process in bone tissue engineering. It is confirmed that in vitro pre-vascular work is used for bone tissue engineering, especial y for in vivo survival and ossification of tissue-engineered bone, pointing out the developmental direction for the clinical application of tissue-engineered bone.

0.05).10% FBS induced -TdR incorporation increased by 87.5%(vs control,P