Objective To explore effectiveness of combination of traditional Chinese medicine and western medicine therapy for patients with Barrett esophagus (BE).Methods Patients with BE were divided into the control group and observation group according to random number table.The observation group was received treatment of com-bination of argon plasma coagulation (APC)and traditional Chinese medicine,while the control group was received treatment of APC alone.Clinical effectiveness was observed at 3,6,12,18months after treatments,recurrence rate was observed 6,12,18months after treatments.Results Clinical effectiveness were significantly higher in the observation group than in the control group 3,6,12,18months after treatments (χ2 =4.71,3.55,7.28,5.10,all P <0.05). Recurrence rate were significantly lower in the observation group than in the control group 6,12,18 months after treat-ments (χ2 =5.84,6.51,all P <0.05).Conclusion Combination therapy of traditional Chinese medicine and west-ern medicine shows confirmed effects to BE.Low recurrence rate in addition makes it become a promising clinical therapy in treatment of BE.

Objective To observe the influence of post-operative swallowing painwith Disposcope endoscope or Macintosh laryngoscope in patients with difficult visible glottis. Methods Thirty-six patients scheduled for elective surgery under general anesthesia whose glottis was not visible underMacintosh laryngoscope (according to Cormach-Lehane Grading of laryngoscopic view) were randomized into 2 groups (n = 18 each):Disposcope endoscope group (group D) and Macintosh laryngoscope group (group M). The rate of glottis exposure and successful intubation, swallowing pain at 30 min, 1, 3 d after extubation were observed. Results Compared with group M , the successful rate of glottis exposure and first intubation was significantly higher than thatin group D (P < 0.05). Swallowing pain VAS was higher in group M than in group D at 1d afterextubation (P < 0.05). Conclusions Compared with Macintosh laryngoscope , Disposcope endoscope in patients with difficult visible glottis could induce higher successful rate of glottis exposure and first intubation , shorter time of intubation and lower VAS of swallowing pain at 1d after extubation.

BACKGROUND:Currently, the implant surface is optimized to promote the osseointegration of artificial dental implants, which has become a research focus in recent years. But the femtosecond laser is less reported to modify the titanium surface so as to promote fibroblast proliferation and differentiation. OBJECTIVE: To observe the effect of femtosecond laser on the proliferation and differentiation of human periodontal ligament fibroblasts on titanium surface. METHODS: Human periodontal ligament fibroblasts were respectively inoculated on different titanium surfaces of the three groups (femtosecond laser group, TiO2 particle blasted group and pure titanium group). Then differentiation and proliferation of human periodontal ligament fibroblasts were observed. RESULTS AND CONCLUSION:There was a significant difference in the surface roughness among these three groups, and compared with the pure titanium group, the attached cels were more in the femtosecond laser group and TiO2 particle blasted group. mRNA expressions of RUNX2 and OSX were highest in the femtosecond laser group folowed by TiO2 particle blasted group and pure titanium group in order. These findings indicate that pure titanium treated with femtosecond laser has fine biological property and may be more feasible for human periodontal ligament fibroblasts to attach, grow and proliferate.

Objective To observe the clinical efect of Weisu particle combined with Mosapride in the treat-ment of the functional dyspepsia .Methods According to the digital table ,64 patients were divided into two groups in random,32 cases were treated by Weisu particle combined with Mosapride in treat group ,33 cases treated by Mosa-pride in control group .Both have a course of 4 weeks treatment .Results The efective rate of the treat ment group′was/96.87%which was better than that of the control group′(s 68.75%)(χ2=4.68,P<0.05).Conclusion The thera-peutic method of Weisu particle combined with Mosapride have a better clinical effect than that of domperidone only used .

Objective To study effect of diclofenac sodium on content of prostaglandin E 2 KC/MC ratio and hyperpigmentation of the full-thickness skin autografts. Methods Content of PGE 2 was determined by radioimmune analysis method, and kinetic changes of KC/MC ratio were observed with histological and histochemical methods. Results In the operation control group, increase of KC/MC ratios was accompanied by of PGE 2 content at the early stage of the experiment; and the KMR became smaller than that in normal control group at the end of the experiment. While in the diclofenac soduim group, KMR was reduced due to the less damage of MC at the early stage, with decrease of PGE 2. Accordingly, KMR became larger at the late period of the experiment, and color of the autografts became lighter. Conclusion Application of diclofenac sodium on the grafts just after transplantation may reduce the damage of inflammation to the epidermal melanin units, control their proliferation and decrease the value of KMR, which lightens the color of the grafts.

Objective To study the short-term therapeutic effects of adeno-associated viral gene transfer of sarcoplasmic reticulum Ca2+-ATPase (SRCA2a) on chronic heart failure (CHF). Methods The rats were divided into four groups: control group, CHF group, CHF+eGFP group and CHF+SRCA2a group. CHF rats were obtained by creating descending aortic constriction. 0.9% sodium chloride solution, recombinant adeno-associated virus carrying enhanced green fluorescent protein (eGFP) gene, and recombinant adeno-associated virus carrying SRCA2a gene, were respectively injected into pericardium of heart failure rats in different groups by intrapericardial injection via trans-diaphragmatic approach. 10 days after gene transfer, hemodynamic parameters, the protein expression of SRCA2a and the SRCA2a activity were measured and analyzed respectively. Results 10 days after gene transfer, the protein expression and activity of SRCA2a were increased by 80.7% and 89.9% respectively, but still lower than those in the control rats. The heart function was also improved along with the increase of SRCA2a expression and activity. The left ventricular systolic pressure and the maximal rate of decline of left-ventricular pressure in CHF+SRCA2a group were similar with those of the controls. The left ventricular end-diastolic pressure and the maximal rate of rise of left-ventricular pressure were much improved, but did not reach the level of the controls. Conclusions SRCA2a protein levels and activity are significantly decreased in CHF rats. Adeno-associated viral gene transfer of SRCA2a can improve the function of the heart in short term.

0.05).10% FBS induced -TdR incorporation increased by 87.5%(vs control,P

Objective To compare the effective difference of the flying needle with intradermal needle among the shu-acupoint group , the estazolam group and the non- point acupuncture group. Methods Three undred and fifteen patients who met the inclusion criteria were randomly enrolled into three groups for the insomnia severity index (ISI) and rating changes detection. After 2-week treatment,the patients were followed up at 4 weeks later. Results The ISI decreased gradually at 1 week, 2 weeks, and 4 weeks after treatment.The therapeutic effects of the three groups were all better than ever before at 1 week, 2 weeks, and 4 weeks after treatment (P<0.05). The follow-up therapeutic effect of the flying needle acupuncture group was better than that of the estazolam group and of the non-point acupuncture group (P<0.05). The curative effect of flying needle acupuncture is better than that in the other two groups (P < 0.05). Conclusion The subjective sleep quality of insomnia patients received flying needle with intradermal needle in shu-acupoint is beter than that of insomnia patients of the Estazolam group and of the non-point acupuncture group.

Objective To evaluate the therapeutic effect and safety of Huangqi Danshen Drop Pill (HDP) for angina pectoris (AP) due to coronary heart disease (CHD) classified as qi- deficiency and blood- stasis syndrome. Methods A multi- center, randomized, double- blind, double- simulation, positive- controlled, parallel group trial was adopted. 209 cases of AP due to CHD classified as qi- deficiency and blood- stasis syndrome were enrolled in this study: 104 in HDP group and 105 in Yangxinshi Tablets group. Results The markedly effective rate was 43.27 % , total effective rate 86.53 % , and total effective rate in improving TCM symptoms 92.31 % in HDP group, which were better than those in Yangxinshi Tablets group(P

BACKGROUND:Preadipocyte is a kind of specific precursor cells with the potential of proliferation and differentiation into adipocytes.If factor or drug with strong promotion of preadipocyte proliferation and differentiation is obtained and used in adipose tissue transplantation,which could elevate transplantation outcome of adipose tissues.OBJECTIVE:To find an optimal culture environment and to accumulate culture experience of in vitro culture 3T3-L1 preadipocytes,and to observe the effect of insulin-like growth factor(IGF)-1 on the proliferation and differentiation of 3T3-L1 preadipocytes,and to explore the optimal concentration of IGF-1.DESIGN,TIME AND SETTING:The cell control observation was performed at the Science Experiment Center,Liaoning University of Traditional Chinese Medicine from October 2007 to February 2008.MATERIALS:3T3-L1 preadipocytes were purchased from Shanghai Institutes For Biological Sciences.IGF-1 was bought from Biological,USA.METHODS:According to in vitro culture direction of 3T3-L1 preadipocytes,3T3-L1 preadipocytes were divided into 6 groups.3T3-L1 preadipocytes in the blank control group were inoculated in high-glucose DMEM supplemented with 0.1 volume fraction of fetal bovine serum.3T3-L1 preadipocytes in each experimental groups were respectively treated with high-glucose DMEM containing 5,10,20,50,100 ?g/L IGF-1 and 0.1 volume fraction of fetal bovine serum.MAIN OUTCOME MEASURES:Growth,proliferation and differentiation of 3T3-L1 preadipocytes in each experimental groups under the inverted microscope,and photographed.When cells were confluence,cell cycles were detected by flow cytometry.Growth rate of 3T3-L1 preadipocytes was measured by MTT essay.Fat growth rate in 3T3-L1 preadipocytes were examined by using oil red O stained-extracted assay.RESULTS:Inverted microscope showed that 3T3-L1 preadipocytes before differentiation were fusiform shape,with little fat in their endochylemas.3T3-L1 preadipocytes after differentiation were spherical shape,with much fat in their endochylemas.Lipid droplet gathered in the perinuclear space,and was stained orange by oil red O stained-extracted assay,forming ring-shape.Results from MTT essay and oil red O stained-extracted assay demonstrated that growth rate of 3T3-L1 preadipocytes and fat growth rate in 3T3-L1 preadipocytes were higher in each experimental groups treated with different doses of IGF-1 compared with the blank control group(P