With the rapid development of new media,medical students' ideological and moral qualities,physical and mental health have been unprecedentedly affected.Traditional ideological and political education modes for medical students encounter great challenges.In order to strengthen the ideological and political education for medical students,we need to focus on three aspects,namely enhancing information qualities of the subjects of ideological and political education,enhancing the supervision of public opinion and guidance on new media and building new interactive environment.

Objective To determine if the cardiac index (CI) measured with arterial pressure-based cardiac output (APCO) agrees with that measured with pulmonary artery catheter (PAC) during cesarean section in parturients with congenital heart disease and severe pulmonary hypertension.Methods Thirty parturients with congenital heart disease and severe pulmonary hypertension,scheduled for elective cesarean section,were enrolled in the study.APCO and PAC were used for cardiac output and other parameters of hemodynamics monitoring during operation in all patients.Continuous epidural anesthesia was performed.CI was recorded immediately before epidural administration (T1),at 5 and 10 min after epidural administration (T2-3),immediately before delivery (T4),and at 2 and 5 min after delivery (T5-6).The correlation was tested by Pearsone correlation analysis.CI derived from APCO and PAC was compared by Bland-Altman analysis for agreement.Results CI measured with PAC was significantly higher than that measured with APCO (P ＜ 0.05).Compared with the baseline value at T1,no significant difference in CI measured with PAC and APCO was found at T11-T4 and T6 (P ＞ 0.05),and CI measured with PAC and APCO was significantly increased at T5 (P ＜ 0.05).Bland-Ahman analysis showed poor agreement between CI measured with the two methods.The correlation analysis showed that there was positive correlation between CI derived from the two methods at T1-6 (the correlation coefficient was 0.93,0.95,0.94,0.89,0.96 and 0.91 at T1-6,respectively,P ＜ 0.05).Conclusion CI value obtained with APCO agrees poorly with that obtained with PAC during cesarean section in parturients with congenital heart disease and severe pulmonary hypertension,but agrees well in monitoring the changing trend of CI.

Objective To study the effect of Guiji cream in the treatment of fissure and permeability of skin.Methods The models of fissure Japonic rabbits were established to observe the effects of skin cream on inhibiting fissure.Rabbits back area was divided into 6 blocks and given different medicines in six different groups:GuiJi cream group of low dosage,middle dosage and high dosage (0.05、0.1、0.2 g/m-2 crude drug of Bletilla colloid respectively),urea frost cream group(0.1 g/m-2),normal saline group and control group.Observe the changes of skin surface symptoms every day.The permeability test was performed on 36 KM mouse and randomly divided into control group,GuiJi cream group and urea frost cream group(0.2 g/m-2).Apply drugs to the back and observe the permeability change of cilia capillary,and auricle swelling degree in mice of each group.Results The curative criteria for Guiji groups was 3.8～4.1,the inhibition rate of auricular swelling was 75％.GuiJi cream could inhibit xylene-induced ear edema (P＜0.05) ; the Inhibition rate of vascular permeability was 45.08％,The ear swelling induced by xylene and increased capillary permeability induced by acetic acid in mice were inhibited significantly (P＜0.05) by Guiji cream which had obvious effects on fissure as well as the permeability.Conclusion Guiji chapped skin cream played significant functions in treating fissure and acting anti-inflammatory.

Objective To investigate the chemokine 12 (CXCL12) and chemokine receptor 4 (CXCR4) expressions in hypopharyngeal carcinoma and its place in the disease development,invasion and metastasis of significance.Methods Immunohistochemistry was used to detect the expressions of CXCL12 and CXCR4 in 35 cases of hypopharyngeal cancer tissues and in 28 cases of tumor-adjacent non-tumor tissues.Results The expressions of CXCL12 and CXCR4 in the hypopharynx carcinomas were significantly higher (P ＜ 0.05).Both expressed in hypopharyngeal carcinomas was significantly positively correlated (P ＜ 0.01).Both hypopharynx cancer in lymph node metastasis group were significantly higher than the expression of cervical lymph node metastasis group,the difference was significant (P ＜ 0.05).Conclusions CXCL12 and CXCR4 are involved in hypopharynx cancer development,invasion and metastasis,and there is a positive feedback regulation mechanism between two factors.Moreover,CXCL12 and CXCR4 have synergistic effect in development,invasion and metastasis of hypopharynx cancers.

Objective To develop an LC-MS/MS method for simultaneous determination of metolazone and valsartan in beagle dog plasma.Methods The chromatographic separation was achieved on an Agilent Poroshell 120(2.1 mm ×30 mm × 2.7 μm)analytical column.The multiple reaction monitoring mode operating in positive ion was adopted in MS detection, and precursors to the product ion transitions of m/z 366.2/259, 436.2/291 and 423.4/207 were used to measure metola-zone, valsartan and internal standard ( losartan potassium) .Results The method was linear over the concentration range of 0.5 ng/ml-100 ng/ml for metolazone and 5-5000 ng/ml for valsartan, with the correlation coefficients ( r2 ) of 0.9937 and 0.9939, respectively.The average intra-day precision values ( RSD) were 2.09% -8.85% for metolazone and 2.36%-13.12%for valsartan.The matrix effect values were 87.73%-98.62%for metolazone and 99.03%-137.35%for valsartan.The average recovery was 75.74%-81.82%for metolazone and 83.89%-95.64%for valsartan.Conclu-sion This analytical method for metolazone and valsartan is simple, accurate and sensitive, so it can be used for pharma-cokinetic research of metolazone and valsartan immediate release tablets in beagle dogs.

Objective To investigate the influence of self-management on perioperative-related symptoms and quality of life in patients with hepatobiliary surgery .Methods Totals of 120 patients with hepatobiliary surgery were randomly divided into the control group and the intervention group by using a random number table , with 60 cases in each group .Patients in the control group received routine clinical care , while those in the intervention group were used the self-management .Patients ’ perioperative-related symptoms were surveyed by the Chinese Version of the Memorial Symptom Assessment Scale ( MSAS-Ch) on the first admitted to the hospital, the seventh day after surgery and one day before discharge .Patients’ quality of life was surveyed by the WHO Quality of Life Scale on the first admitted to the hospital and one day before discharge .The scores of symptoms and quality of life were compared before and after intervention .Results The scores of MSAS-Ch and quality of life before the intervention were (0.82 ±0.36)and (11.15 ±3.11) in the control group, and (0.79 ±0.34) and (11.77 ±3.01) in the intervention group.There were no significant differences between two groups (t=0.469, 1.110, respectively;P>0.05).In the control group, the scores of symptoms on the first admitted to the hospital, the seventh day after surgery and one day before discharge were (0.82 ±0.36), (0.84 ±0.23) and (0.62 ±0.18), respectively.Those scores of the intervention group were (0.79 ±0.34), (0.43 ±0.21) and (0.18 ±0.09),respectively.The differences of time effect , group effect and interaction effect were statistically significant (P<0.05).After the invention, the score of physiology dimension in the control group and the total score and other six dimensions in the intervention group were significantly higher than those before the invention (P<0.05).Except for physiology dimension , the total score of quality of life and scores of other five dimensions in the intervention group were significantly higher than those in the control group (P<0.05).Conclusions Self-management can effectively reduce the level of diseased -related symptoms in patients after hepatobiliary surgery and improve patients ’ quality of life.

Objective To explore the effect of the nursing risk assessment and early warning intervention mode in decreasing the risk of the clinical administration .Methods The risk management theory was applied in the nursing management of clinical administration , and the nursing risk of clinical administration was effectively evaluated through the failure mode and effect analysis of the risk management tool .The early warning intervention of different grades was carried out according to the Risk Priority Number ( RPN) .Results The total value at risk of administration after the intervention was decreased by 39.58%.The incidence rate of nursing adverse events caused by the administration was 0.06% after the intervention, and was lower than 0.14% before the intervention, and the difference was statistically significant (χ2 =4.67,P<0.05).Conclusions Application of the nursing risk assessment and early warning intervention can decrease the incidence rate of nursing adverse events caused by the administration so as to ensure the safety in patients .

Non-coding RNA (ncRNA) have enormous regulatory functions and play important roles in a variety of cellular processes including cell growth, differentiation, survival and apoptosis. Influenza A virus (FluA) is an important pathogen of serious threats to human health and livestock industry. The interaction mechanism between FluA and the host is very complicated. FluA infection can lead to differential expression of hundreds of host ncRNA, which has become a research hotspot in life sciences in recent years. This review summarizes the progress in different host-encoded ncRNA involved in regulating FluA infection, not only to study the interaction between viruses and hosts, but also to discover new antiviral strategies.

Objective: To observe the activation of microglia and the changing rule of inflammatory cytokine as IL-6, IL-10 and nuclear factor-κB (NF-κB) in experimental rabbits after spinal cord ischemia reperfusion (SCIR) injury in order to provide theoretical basis for post-conditioning time. Methods: Rabbit SCIR injury model was established by thoracic aorta balloon occlusion. 54 New Zealand male adult white rabbits were divided into 9 groups: Sham group (the animals received balloon implantation without occlusion), SCIR-0h group (reperfusion was conducted at 0 hour of spinal cord ischemia), SCIR-1h, -2h, -3h, -8h, -24h,-48h and -72h groups. n=6 in each group. The number of normal and apoptosis neurons, the levels of Iba-1, IL-6, IL-10 and NF-κB in spinal tissue were examined and compared among different groups respectively. Results: The number of normal neuron was decreasing with the extended reperfusion time, TUNEL-positive neuron began to increasing in SCIR-8h group and the peak was reached in SCIR-24h group. The expression of Iba-1 began to elevating in SCIR-2h group and the peak was obtained in SCIR-8h group; NF-κB began to rising in SCIR-3h group and the peak was observed in SCIR-8h group; both IL-6 and IL-10 arrived the peak in SCIR-24h group. The expressions of NF-κB, IL-6 and IL-10 were positively related to Iba-1 level. Conclusion: Microglia activation had dynamic changes in experimental SCIR rabbits and the expression levels of NF-κB, IL-6 and IL-10 were positively to microglia activation; post-conditioning time at front and back to microglia activation may reduce neuron injury.

Objective: To explore sarcoplasmic reticulum ryanodine receptor2 (RyR 2) expression and calcium releasing function in chronic heart failure (CHF) rabbits and to study the impact of long term valsartan treatment in relevant animals. Methods: HF model was established by volume overloading with pressure overloading in experimental rabbits. 27 rabbits were divided into 3 groups: Sham group, HF group and HF+valsartan group. n=9 in each group and the animals were treated for 7 weeks. Left ventricular structure, hemodynamic parameters, expression and functional changes of myocardiocyte sarcoplasmic reticulum RyR 2 were observed and compared among different groups. Results: Compared with Sham group, HF group had increased left ventricular mess index (LVMI), left ventricular end diastolic pressure (LVEDP) and decreased left ventricular shortening fraction, LVEF, all P<0.05. Compared with HF group, HF+valsartan group showed decreased LVMI, LVEDP and increased left ventricular shortening fraction, LVEF, all P<0.05. Sarcoplasmic reticulum RyR 2 expression and calcium releasing function were lower in HF group than Sham group, P<0.05; while they were both higher in HF+valsartan group than HF group, P<0.05. Conclusion: Long term application of valsartan could improve the cardiac function which might be related to increased myocardial sarcoplasmic reticulum RyR 2 expression and calcium releasing function in experimental CHF rabbits.