Superoxide dismutase was prepared from human crythrocytes. The sample was purified using a DEAE-cellulose column and chromatography on Sephadex G-75. During purification the produce determined the protein peak and SOD active peak at the same time to decide the SOD was present. The enzyme exhibited an ultraviolet absorption spectra with a maximum at 270-275 nm. Polyacrylamide disc gel electrophoresis revealed three bands which were in dose agreement with the active bands by reduction of NBT. The molecular weight was 41687, 31623 and 15136. The specific activity of human SOD possessed 3300 units per mg It contained 2 eq of Cu, Zn per mole of enzyme, respectively.

ObjectiveTo compare and analyze conventional plan and inverse optimized plan in dosemetric of cervical cancer. MethodTwenty cases of cervical cancer treated with combination radical radiotherapy of EBT were selected,every case had two plans: one was conventional plan based A point prescription dose, the other was inverse optimized plan (IPSA, inverse planning with simulated annealing)based volume object dose.ResultsIPSA plans provided better values compared with the conventional plans in 90％ prescription dose volume V90[ (94 ± 15 )％ vs. (60 ± 17 )％], 100％ prescription dose volume V100[(90 ± 18)％ vs. (56 ± 14)％]and 100％ treatment volume dose D100[(54 ± 10)％ vs. (29 ±9)％](P ＜0.05),respectively. Meanwhile the organ at risk received lower dose volume. ConclusionsPlans generated using IPSA provide higher dose to the target volume but with lower dose to normal structure and less time. This study can help to guide the clinical application.

Objective To evaluate the CT findings of invasive pulmonary aspergillosis(IPA) in patients with leukemia.Methods Plain CT scanning of chest was performed in 9 cases of leukemia in company with IPA proved by sputum culture.5 of them underwent over twice CT re-scanning,CT findings of IPA were analysed.Results The lesions were seen more common in the bilateral upper lobe and dorsal segment of lower lobe,mainly located in the peripheral and sub-pleural lung field.Of the 9 cases,the lesions appeared as multi-nodular shadows in 5,single nodule in 2 and sub-pleural consolidation with ground-glass opacity around them in 2,of these 2 cases,one accompanied by multi-nodules.Halo-sign around the nodules in 6 cases and air cresent sign or cavity in 6 were seen.There were scattered ground-glass opacities and patchy attenuations in the bilateral lung in 4 cases.The adjacent pleura of lesion was slightly thinkened in 5 cases.Conclusion The incidence of IPA in leukemia patients is rather high,and CT features of IPA are of certainly characteristics.

Objective To evaluate the correlation between the contrast enhanced features on spiral computed tomography(SCT) and microvessel density(MVD),TP,TGF-?_1 expression.Methods Forty seven cases with hepatocellular carcinoma(HCC) proved pathologically were examined with enhanced dual-phase SCT scanning at the arterial phase and the portal vein phase.The SCT features including the size of HCC lesions,enhanced patterns,capsule patterns,the diameter of tumor,intrahepatic daughter foci and tumor-emboli in portal veins were studied.The expression of TP,TGF-?_1 and MVD were detected by immunohistochemical SP method.The enhanced features of SCT were compared with the immunohistochemical results and clinicopathological characteristics of HCC.Results TP and MVD were correlated with metastasis and capsule patterns(P0.05).Conclusion The expression of TP,TGF-?1 and angiogenesis of HCC can be evaluated by some SCT features in a certain degree.

BACKGROUND:Conventional imaging method has significant limitations in the diagnosis of knee meniscus injury, which is difficult to detect the ful extent of the knee meniscus. OBJECTIVE:To evaluate the knee meniscus injury with magnetic resonance imaging. METHODS:The knee meniscus injury patients diagnosed with clinical treatment were retrospectively analyzed, and the characteristics of the magnetic resonance imaging were analyzed and graded. Then, the meniscus injury was analyzed and compared with the arthroscopic or surgical results. RESUTLS AND CONCLUSION:In the magnetic resonance imaging of knee meniscus injury, the Ⅰ level knee meniscus injury was presented as the punctate or round high signal that did not contacted with the surface of the meniscus, Ⅱ level knee meniscus injury was presented as the horizontal or oblique line or strip-like high signal in the meniscus, and Ⅲ level knee meniscus injury was presented as lines and complex high signal in the meniscus that extended to the articular surface of the meniscus, and accompanied with morphological changes of the meniscus. Magnetic resonance imaging, as a noninvasive checking method, can clearly show the meniscal degeneration and tearing, and correctly diagnosed the location, shape and severity of meniscus injury, which has significant guiding value in determine the clinical treatment options.

Objective To observe the effect of electroacupuncture at Baihui (GV20) and Shenting (GV24) on learning-memory function and ultrastructure in hippocampal CA1 region of rats after cerebral ischmeia-reperfusion. Methods A total of 25 male Sprague-Dawley rats were randomly divided into sham group (n=6) and operation group (n=19). The operation group was occluded the left middle cerebral arter-ies with modified Longa's methods for 90 minutes and reperfused, and twelve qualified rats of them were randomly divided into model group (n=6) and electroacupuncture group (n=6), the later accepted electroacupuncture at Baihui and Shenting for seven days. They were as-sessed with Longa's scores, and tested with Barnes maze. Their cerebral infarct volume was tested with magnetic resonance imaging T2-weighted image. The ultrastructure of synapse in hippocampal CA1 region was observed with transmission electron microscope. Results Compared with the model group, the Longa's score improved (P<0.05), the infarct volume decreased (P<0.01), the average escape latency decreased (P<0.01) and the times entering the wrong hole decreased (P<0.001) in the electroacupuncture group. Under the transmission elec-tron microscope, the number of synapse decreased in the model group, with the structure damage and vesicles sparse;compared with the model group, the number of synapse increased in the electroacupuncture group, with clear and complete structure and rich vesicles. Conclu-sion Electroacupuncture at Baihui and Shenting can improve the learning-memory function in rats after cerebral ischmeia-reperfusion, which may relate to improvement of synaptic plasticity and ameliorating ultrastructure in hippocampal CA1 region.

Objective To study the correlation between the polymorphisms of NR3C1 gene and aggressive behavior in Yunnan Han population.Methods Five SNPs of the NR3C1 gene (rs6190,rs6191,rs6198,rs41423247 and rs56149945) were genotyped in 194 unrelated prisoners who committed violent-crimes and 301 healthy controls using improved Multiplex-ligase-detection reaction(iMLDR) method,and the data were statistically analyzed with the SPSS19.0soflware and PHASE2.1platform.Results Single locus analysis showed that the allelic distribution of rs6191and rs41423247did not show significant differencesbetween the control groupand the aggressive-behavior group as well as the robbery sub-group and intentional injury sub-group.However,significant difference was foundin the rs41423247 genotype distribution betweencontrol groupand robbery sub-group (p=0.048).In addition,there were no significant differences for the four haplotypes between the control group,the attack group,the robbery subgroup and the intentional injury subgroup.Conclusion These findings indicate that rs41423247 polymorphism of the NR3C1gene might play a role in susceptibility to aggressive behavior and rs6191 polymorphismmay not be correlated withaggressive behavior.

Objective To investigate the short-term efficacy and security of combined treatment of iodine-125 seeds and transeathether arterial chemoembolization in liver neoplasms. Methods Transcathether arterial chemoembolization underwent in experimental group (28 cases) with liver neoplasm. The treatment plan was formulated with treatment planning system and a median of 25 seeds per patient (range, 15--40 seeds) were implanted under CT or B ultrasound guidance in 2 weeks after the procedure. Transcathether arterial ehemoembolizafion underwent after the implanted regularity. Blood routine and liver function were detected before and after the procedure. X ray check and abdomen CT scan were performed each 2 months. Control group (32 cases) were treated with transcathether arterial chemoembolization alone. Analysis of variance and Chi-square test were used for statistics. Results All seeds were released to the target places successfully and no seed was found to be lost or migrated in experimental group. Transient elevation of the serum ALT and AST but recovered in 2 week. WBC, Hb, IgA and IgG were showed no significant changes. The severe complication was not found in those eases. The responsive rate of tumor was 75.0% (21/28), 37.5% (12/32) in experimental group and control group, respectively(X2 = 8.485,P = 0.004). The survival rate of 6 months was 92.9% (26/28), 75.0% (24/32) in experimental group and control group, respectively(X2=2.263,P=0.132). The surviral rate of 12 months was 72.0% (18/25), 43.3% (13/30) in experimental group and control group, respectively (X2 = 4.556, P=0.033). Conclusion It is simple, feasible, safe and short-termly effective for liver neoplasms in treatment combined iodine-125 seeds implantation with transcathether arterial chemoembolization.

OBJECTIVETo evaluate the impact of transanal endoscopic microsurgery (TEM) on postoperative anal function and quality of life in patients with benign rectal tumor and early rectal cancer.

METHODSClinical data of 50 patients with rectal adenoma and early rectal cancer undergoing transanal endoscopic microsurgery in our hospital from October 2008 to June 2013 were retrospectively analyzed. Anorectal manometry, endorectal ultrasonography (ERUS), the fecal incontinence severity index (FISI), and the physical and mental health status scores (SF-36) were used to evaluate preoperative and postoperative anorectal function and quality of life.

RESULTSAnorectal manometry indicated anal resting pressure (ARP), maximum squeeze pressure (MSP), rectal volume at sensory threshold(RVST), maximum tolerable volume(MTV) decreased significantly at the first month after surgery (P<0.05). MSP returned to preoperative level at the 3rd month (P>0.05). ARP and MTV returned to normal values at the 6th month (P>0.05). RVST returned to normal values at the 9th month (P>0.05). Recto-anal inhibitory reflex(RAIR) was absent in 1 (2%) patient preoperatively and in 30(60%), 18(36%), 7(14%), 2(4%) at the 1st, 3rd, 6th, 9th months after surgery respectively. ERUS showed similar width and thickness of internal sphincter at 1st and 6th month after surgery compared with preoperative measures (P>0.05). Six months after surgery, the mean FISI score decreased(preoperative vs postoperative:8.5 vs 5.8, P<0.05), suggesting an improvement in fecal continence. However, the overall quality of life did not danger significantly after surgery(P>0.05).

CONCLUSIONSTEM has little impact on anorectal anatomic structure. Anal function may be compromised in the short-term, however the vast majority of patients recover completely after 6-9 months. TEM is a safe, effective and minimally invasive surgery.

Adult ; Aged ; Anal Canal ; physiopathology ; surgery ; Endoscopy ; Female ; Humans ; Male ; Microsurgery ; Middle Aged ; Postoperative Period ; Quality of Life ; Rectal Neoplasms ; surgery ; Retrospective Studies

OBJECTIVETo elucidate the mechanism of curcumin in radiotherapy sensitization for colorectal cancer cells.

METHODSColorectal cancer HT-29 cells were cultured and treated with radiation and curcumin. MTT method was used to detect the cell growth inhibition. Then the high-throughput microarray was used to detect the differences in gene expression levels for each test group to identify differentially expressed genes, and each differential gene was validated by Western blotting.

RESULTSCell growth inhibition rates at 48-hour and 72-hour in curcumin combined with radiotherapy group were significantly higher than those in simple radiotherapy group (P<0.05). Expression of 95 genes associated with gene-injury repair was detected by microarray. Compared to simple radiotherapy group, LIG4 and PNKP expression was down-regulated, and XRCC5 and CCNH expression was up-regulated in the curcumin combined with radiotherapy group (all P<0.05). Western blotting revealed LIG4 and PNKP protein expression decreased, and XRCC5 and CCNH protein expression increased in the curcumin combined with radiotherapy group as compared to the simple radiotherapy group (all P<0.05).

CONCLUSIONRadiation sensitization effect of curcumin on colorectal cancer cells HT-29 may be associated with the regulation of genes of CCNH, LIG4, XRCC5, PNKP.

Blotting, Western ; Cell Line, Tumor ; Curcumin ; Down-Regulation ; Gene Expression ; Gene Expression Regulation, Neoplastic ; Humans ; Rectal Neoplasms