0.05).The ratio of TH~+ cells in the experimental group(7.38?0.84)% was higher than that in the control group(0.53?0.17)%,and there was significant statistical difference between the two group(P

AIM:To study the anti-tumor effect and mechanism of fusion vaccine on DCs and C6 glioma cells.METHODS: PEG was used to fuse DCs with C6 glioma cells.Immunofluorescence with GFAP-FITC was used to identify the DC/C6 fusion cells.Rat brain glioma models were made by stereotactic technique.After 5 days of inoculation of C6,107 fusion cells were injected through tail vein in group A.The same number of DCs and the same volume of PBS were used in group B and group C.The survival time of rats in these three groups was analyzed by Log-rank survival analysis.Tumor samples were checked by HE staining and immunohistochemical staining with CD8Mcab.RESULTS: Positive result of GFAP-FITC immunofluorescence was observed in DC/C6 fusion cells.The Log-rank survival analysis showed that statistically significant difference in group A was observed compared to that in group B and group C(P

Objective: To compare nephrotoxicity and ototoxicity of gentamycin administered in single dose or multiple dose daily in guinea pigs. Methods: Thirty two male guinea pigs were divided into physiological saline control, single dose group daily (gentamycin, 120 mg/kg, 1/d) and multiple dose group daily (gentamycin, 60 mg/kg, 2/d). The physiopathology of renal and cochlea in guinea pigs were examined using auditory brainstem response (ABR), SC sound irritation and electron microscope. The gentamycin concentrations in serum and in perilymph were monitored by fluorescene polarization immunoassay (FPIA). Results: (1) Compared with control group, both gentamycin single and mulitiple daily doses injuried kidney and cochlea to some extent.The injury of multiple dose groups were worse than that the single dose groups ( P

Objective: To evaluate the effect of new media among women with gestational diabetes mellitus (GDM) during postpartum period. Methods: The gestational diabetes maternal in the obstetrics department of Peking University First Hospital from May to June, 2017 was selected. We used excel generated random number table to divide the groups, there were 50 cases in the control group and 46 cases in the study group. The control group used routine postpartum routine health education, including informing 2 groups of maternal weight management diet and exercise plans, giving leaflets, and conducting telephone to supervise the quality control; based on these situations, the study group added group WeChat push reminders and Q&A, and "317 Protector" to push the heath education materials after discharge. Then we did comparison of oral glucose tolerance test (OGTT), lipid test results and body mass index (BMI) of the two groups on the 42nd day postpartum. Results: The recovery and control of the study group women were better than those of the control group. In the study group, BMI, fasting blood glucose, triglyceride, total cholesterol, high-density lipoprotein, and low-density lipoprotein were (22.13±2.91) kg/m², (5.06±0.39) mmol/L, (0.89±0.27) mmol/L, (5.61±0.68) mmol/L, (1.52±0.27)mmol/L, (3.16±0.71) mmol/L after 42 days of birth. While in the control group, these were (23.66±4.21)kg/m², (5.30±0.47) mmol/L, (1.03±0.36) mmol/L, (5.64±0.66) mmol/L, (1.63±0.30) mmol/L, (3.23±0.62) mmol/L. The difference between the two groups was statistically significant (t value was -3.604-2.059, P<0.05 or 0.01). Conclusion New media for postpartum follow-up of gestational diabetes can effectively help improve BMI, blood lipids and other indicators, and can also help blood sugar control effect.