Objective To study the influence of intravenous injection of dezocine and fentanyl under general anesthesia on analepsia and analgesia after children appendectomy.Methods 86 children needed peritoneoscope appendectomy were enrolled in the study.All patients were randomized into two groups.The patients in group A were given intravenous injection of dezocine at half an hour before the end of the operation.The patients in group B were given intravenous injection of fentanyl before the end of the operation.The operation length,the postoperative catheter removal time and the heart rate,respiratory rate,mean arterial pressure,blood pressure saturability at the time of stopping using narcotic (T1),the time of catheter removal (T2),10min after catheter removal (T3) of the two groups were observed and recorded.At half an hour after catheter removal,Riker sedation-agitation scale and FLACC of two groups were estimated.Results The heart rate[(112.47±8.06)beats/min,(111.29±9.03)beats/min] and mean arterial pressure[(69.49±7.33)mmHg,(68.72±6.93)mmHg] of group A at T2,T3 were significantly lower than those of group B[heart rate: (126.98±7.24)beats/min,(131.54±8.62)beats/min and mean arterial pressure: (76.81±5.18)mmHg,(72.05±3.76)mmHg, heart rate tT2=3.294,tT3=5.018;mean arterial pressure tT2=4.029,tT3=2.287,all P<0.05].Besides,the FLACC score[(3.62±0.73)points] and Riker score[(2.93±0.75)points] of group A at half an hour after catheter removal were significantly lower than those of group B[(5.06±0.92)points,(3.96±1.17)points,tFLACC=6.390,tRiker=2.119,all P<0.05].Conclusion Intravenous injection of properdezocine at half an hour before the end of the operation has good postoperative analgesia effect for children appendectomy and children wake up in a more stable state,and the occurrence rate of postoperative analgesia and adverse reaction is low, so it has less influence on cardiovascular reaction and is applicable for clinical application.

22 cases of chronic gastritis,8 cases of gastric cancer,and 3 cases of other gastric diseases.10 hea'thy adults were also studied to serve as the control.According to the diagnostic criteria of Chinese traditional medicine ,58 cases with epigastric pains out of the 80 were categorized into the group of spleno-gastric asthenia (SGA) of 35 cases and the group of hepatogastric incoordinatin (HGI) of 23 cases.The basal pressure and rhythmic contractions of the stomach in response to a definite volume loading were recorded.It was found that 60% of SGA cases and 56.8% of PU cases showed only a few low-amplitude or no contractions in response to all varieties of volume loading,which were significantly higher than that in HGI cases (26%) and the control (10%).In the interval between the contractions and relaxations of the proximal stomach the intra-gasrric pressure was significantly lower in SGA and PU cases than in HGI cases and the control.These findings indicate that the tension and contractility of the mesculature of the proximal stomach are markedly reduced in patients with PU and SGA.

Objective To investigate the mechanism of high phosphate - stimulated calcification in vascular smooth muscle cell. Methods The cell culture of bovine aortic smooth muscle was performed. Osteocalcin (OC) mRNA expression and calcium deposition in different phosphate concentrations [normal phosphate (Pi 1. 5mmol/L), high phosphate (2.0mmol/L)] were determined by radioimmunoassay, RT-PCR, and 0-cresolphthalein complexone method respectively. Results After 72 hours,high phosphate treatment of smooth muscle cells(SMC) enhanced the expression of osteocalcin protein [Pi 2. 0 mmol/L versus Pi 1. 5 mmol/L: (15. 03?2. 60) pg/u-g protein versus (2. 98 ?0.84) pg/ug protein, P

OBJECTIVE: To explore the biological characteristics of tendon and performance of repair materials for tendon defects. METHODS: CNKI and Medline database were retrieved by computer for papers published between 1985 and 2009. Literature concerning biological characteristics and performance of repair materials for tendon defects were selected. Meta analysis, irrespective papers or repetitiveness studies were excluded. Totally 29 papers were included after screening and evaluating including 16 in Chinese and 13 in English. The biological characteristics of tendon and performance of repair materials were summarized.RESULTS: Nutrition for tendon mainly supplied by blood, and the tendon fatigue would be occurred with repeatability loads. Compared to other tissue-engineered materials, tendon possesses self-repair ability. The biological characteristics of tendon was closely associated with collagen fiber, which exhibited similar stress-strain curves, namely, characteristics of viscoelasticity body--hysteresis, creep and stress relaxation. Currently, main repair materials for tendon defects comprised autogenous tendons, allogeneic tendon, artificial tendon and tissue engineered tendon.CONCLUSION: Many materials can be used in tendon repair with distinct advantage and disadvantage; however, there is not an ideal repair method. With the development of tissue and gene engineering, the optimal substitute for tendon would be prepared.

AIM To observe the effect of phosphonoformic acid (PFA) in different concentration on vascular calcification induced by elevated phosphate. METHODS The cell culture of bovine aortic smooth muscle were performed. Calcium deposition in different phosphate and PFA concentrations were determined by O cresolphthalein complexone method, and osteocalcin expression by radioimmunity and RT PCR. RESULTS Compared to Pi 1 5 mmol?L -1 group,bovine smooth muscle cells (BSMC) cultured in medium containing Pi 2 0 mmol?L -1 phosphate level increased calcium deposition 〔On day 6, (77 187?11 692) mg?g -1 Pro versus (25 768?1 750) mg?g -1 Pro, P

Objective To investigate the correlation of social capital and professional burnout in nursing staff. Methods Totally 1329nursing staff were recruited from 6 hospitals in China. They were investigated with the social capital scale and nursing personnel professional burnout scale. Results The mean score of social capital was 13.37±1.30;the mean score of 3 Scales of professional burnout was 24.39± 9.98, 6.24 ± 4.78, 23.37 ± 10.12. The 4 Scales scores of social capital were negative correlated with emotional exhaustion scale, the lack of human individual scale(r=-0.564 to-0.211,P<0.05). Besides with trust and security dimension, low sense of achievement were positively correlated with the total score of social capital (r=0.281-0.281, P<0.05). Conclusion Social capital of nursing staff have important effects on nurse's professional burnout. Nursing managers should take effective measures to reduce nurse's professional burnout.

Objective To investigate the effect of hyperphosphatemia on type Ⅱa sodium dependent phosphate cotransporter (NaPi-2)mRNA expression in the kidneys of 5/6 nephrectomized (STNx)rats and evaluate the interference of Renagel. Methods Forty-two male Sprague-Dawley rats underwent 5/6 nephrectomy or sham operation. The animals were then divided into seven groups: (1)STNx+high-Pi diet(HP) group (1.2%P); (2)STNx+low-Pi diet(LP) group(0.2%P); (3)STNx+ normal-Pi diet (NP) group; (4)STNx+HP+Renagel-treated (Ren)group; (5)sham+HP group; (6) sham+LP group; (7)sham+NP group. Serum ionized calcium (iCa) , phosphorus, and iPTH levels were detected at day 2,7,14.The kidneys were removed after the rats were sacrificed on day 14 and serum 1,25-(OH)2D3 and FEp were measured. RT-PCR was applied to examine NaPi-2 mRNA. Results Serum P and iPTH levels in STNx+HP group were significantly higher than those in STNx+ LP and 3 sham groups (P0.05)among these five groups mentioned above. Serum P and iPTH levels in Renagel-treated group were much lower than those in STNx+HP group (P

MicroRNAs (miRNAs) play important roles in eukaryotes,plants and some viruses.It is increasingly clear that miRNAs-encoded by viruses can affect the viral life cycle and host physiology.Viral miRNAs could repress the innate and adaptive host immunity,modulate cellular signaling pathways,and regulate the expression of cellular and viral genes.These functions facilitate viral acute and persistent infections,and have profound effects on the host cell survival and disease progression.Here,we discuss the miRNAs encoded by herpesviruses,and their regulatory roles involved in virus-host interactions.

Objective To evaluate the accuracy of vasopressin (VP) secretion in the late phase of septic shock for predicting patient outcomes and further investigate its relationship with the prognosis of septic shock.Methods Fifty-five patients presented at late phase of septic shock,who were admitted to the intensive care unit of our hospital,were enrolled.Their VP secretion was measured.The method for measurement was as follows:3％ sodium chloride solution 600 ml was infused over 2 h,serum concentrations of VP and sodium were measured before and after infusion,the difference in VP before and after infusion (△VP) and in Na before and after infusion (△Na) was calculated,and △VP/△Na was used to reflect VP secretion.The patients were divided into either abnormal secretion of VP group (△ VP/△ Na ≤ 0.5 ng/mmol) or normal secretion of VP group (△VP/△Na＞0.5 ng/mmol) according to △VP/△Na ratio.Immediately before testing VP secretion,venous blood samples were collected for determination of serum lactic acid and C-reactive protein concentrations.The consumption of vasoactive drugs at the moment of enrollment and 28-day fatality rate were recorded.Results There were 30 cases in abnormal group (54％) and 25 cases in normal group (46％).Compared with normal group,the serum lactic acid,C-reactive protein concentrations and consumption of dopamine or norepinephrine were significantly increased,and the 28-day fatality rate was increased (67％ vs 40％) in abnormal group.ROC curve analysis showed that when △VP/△Na 0.5 ng/mmol was used as the criteria for determining prognosis,the sensitivity was 66.7％,specificity was 64.0％,and the area under the ROC curve was 0.828.Conclusion VP secretion in the late phase of septic shock may affect patient prognosis.

Objective To improve the diagnosis and treatment of infective endocarditis(IE)by exploring its causes,pathogenic microorganism and clinicsI characteristics.Methods The clinical data of 120 IE patients treated in Peking Union Medical College Hospital from October 1997 to September 2007 were analyzed retrospectively.Results Of the 120 consecutive cascs diagnosed as IE according to the Duke's new criteria,79 were male and 41 female with a average age of(43.2±16.7)years old.Twelve cases were prosthetic valve endocarditis(PVE)and 108 cases native valve endocarditis(NVE)and there were no previously known heart diseases in 29 of the cases.Seventy-nine of the 108(73.1%)NVE patients had basic cardiac abnormalifies before IE diagnosis,such as congenital cardiovascular disease(30 cases),idiopathic mitral valve prolapse(23 cases)and rheumatic heart disease(11 cases).Fever(100.0%),anemia(54.2%)and embolism(48.3%)were the most common clinical manifestations in the IE development.Of the 83 patients who had a positive blood culture result,Streptococcus vividaus(51.8%)was the most common isolated microorganism.Conclusions Congenital cardiovascular diseases and idiopathic mitral valve prolapse are the two most commonly heart diseases in IE.Blood culture and echocardiogram should always be done to exclude IE,especially presenting with fever of unknown reasons.